Two electrophysiologically distinct types of cultured pacemaker cells from rabbit sinoatrial node
R. D. Nathan Previous investigations employing multicellular nodal preparations (i.e., mixtures of dominant and subsidiary pacemaker cells) have suggested that the fast transient inward sodium current (iNa) either is not present in dominant pacemaker cells or is present but inactivated at the depola...
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| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 1986-02, Vol.250 (2), p.H325-H329 |
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| container_issue | 2 |
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| container_title | American journal of physiology. Heart and circulatory physiology |
| container_volume | 250 |
| creator | Nathan, R. D |
| description | R. D. Nathan
Previous investigations employing multicellular nodal preparations (i.e.,
mixtures of dominant and subsidiary pacemaker cells) have suggested that
the fast transient inward sodium current (iNa) either is not present in
dominant pacemaker cells or is present but inactivated at the depolarized
take-off potentials that these cells exhibit. In the present study, this
question was resolved by voltage clamp analysis of single pacemaker cells
isolated from the sinoatrial node and maintained in vitro for 1-3 days. Two
types of cells, each with a different morphology, exhibited two modes of
electrophysiological behavior. Type I cells (presumably dominant
pacemakers) displayed only a tetrodotoxin (TTX)-resistant (but
cadmium-sensitive) slow inward current, whereas type II cells (presumably
subsidiary pacemakers) exhibited two components of inward current, a
TTX-sensitive, fast transient inward current and a TTX-resistant (but
cadmium-sensitive) slow inward current. Three other voltage-gated currents,
1) a slowly developing inward current activated by hyperpolarization (if,
ih, delta ip), 2) a transient outward current activated by strong
depolarization (ito, iA), and 3) a delayed outward current, were recorded
in both types of pacemaker cells. |
| doi_str_mv | 10.1152/ajpheart.1986.250.2.h325 |
| format | Article |
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Previous investigations employing multicellular nodal preparations (i.e.,
mixtures of dominant and subsidiary pacemaker cells) have suggested that
the fast transient inward sodium current (iNa) either is not present in
dominant pacemaker cells or is present but inactivated at the depolarized
take-off potentials that these cells exhibit. In the present study, this
question was resolved by voltage clamp analysis of single pacemaker cells
isolated from the sinoatrial node and maintained in vitro for 1-3 days. Two
types of cells, each with a different morphology, exhibited two modes of
electrophysiological behavior. Type I cells (presumably dominant
pacemakers) displayed only a tetrodotoxin (TTX)-resistant (but
cadmium-sensitive) slow inward current, whereas type II cells (presumably
subsidiary pacemakers) exhibited two components of inward current, a
TTX-sensitive, fast transient inward current and a TTX-resistant (but
cadmium-sensitive) slow inward current. Three other voltage-gated currents,
1) a slowly developing inward current activated by hyperpolarization (if,
ih, delta ip), 2) a transient outward current activated by strong
depolarization (ito, iA), and 3) a delayed outward current, were recorded
in both types of pacemaker cells.</description><identifier>ISSN: 0363-6135</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1539</identifier><identifier>DOI: 10.1152/ajpheart.1986.250.2.h325</identifier><identifier>PMID: 3946631</identifier><language>eng</language><publisher>United States</publisher><subject>Action Potentials - drug effects ; Animals ; Cadmium - pharmacology ; Cadmium Chloride ; Electric Conductivity ; Electrophysiology ; Male ; Rabbits ; Sinoatrial Node - cytology ; Sinoatrial Node - physiology ; Tetrodotoxin - pharmacology</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 1986-02, Vol.250 (2), p.H325-H329</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-d230652b627c0e13df2a5d1c2b05894faed2a56b759ddcb7254394a9174d31813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3946631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nathan, R. D</creatorcontrib><title>Two electrophysiologically distinct types of cultured pacemaker cells from rabbit sinoatrial node</title><title>American journal of physiology. Heart and circulatory physiology</title><addtitle>Am J Physiol</addtitle><description>R. D. Nathan
Previous investigations employing multicellular nodal preparations (i.e.,
mixtures of dominant and subsidiary pacemaker cells) have suggested that
the fast transient inward sodium current (iNa) either is not present in
dominant pacemaker cells or is present but inactivated at the depolarized
take-off potentials that these cells exhibit. In the present study, this
question was resolved by voltage clamp analysis of single pacemaker cells
isolated from the sinoatrial node and maintained in vitro for 1-3 days. Two
types of cells, each with a different morphology, exhibited two modes of
electrophysiological behavior. Type I cells (presumably dominant
pacemakers) displayed only a tetrodotoxin (TTX)-resistant (but
cadmium-sensitive) slow inward current, whereas type II cells (presumably
subsidiary pacemakers) exhibited two components of inward current, a
TTX-sensitive, fast transient inward current and a TTX-resistant (but
cadmium-sensitive) slow inward current. Three other voltage-gated currents,
1) a slowly developing inward current activated by hyperpolarization (if,
ih, delta ip), 2) a transient outward current activated by strong
depolarization (ito, iA), and 3) a delayed outward current, were recorded
in both types of pacemaker cells.</description><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Cadmium - pharmacology</subject><subject>Cadmium Chloride</subject><subject>Electric Conductivity</subject><subject>Electrophysiology</subject><subject>Male</subject><subject>Rabbits</subject><subject>Sinoatrial Node - cytology</subject><subject>Sinoatrial Node - physiology</subject><subject>Tetrodotoxin - pharmacology</subject><issn>0363-6135</issn><issn>0002-9513</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0N1KwzAcBfAgypzTRxDyAq35aNL2UoY6QfBmXoc0SdfMdClJxujb27E5vQr8D-dAfgBAjHKMGXmS26EzMqQc1xXPCUM5yTtK2BWYTzHJMKP1NZgjymnGMWW34C7GLUKIlZzOwIzWBecUz4FcHzw0zqgU_NCN0XrnN1ZJ50aobUx2pxJM42Ai9C1Ue5f2wWg4SGV6-W0CVMa5CNvgexhk09gEo915mYKVDu68NvfgppUumofzuwBfry_r5Sr7-Hx7Xz5_ZIrwImWaUMQZaTgpFTKY6pZIprEiDWJVXbTS6OnAm5LVWqumJKyYPiFrXBaa4grTBahOuyr4GINpxRBsL8MoMBJHNPGLJo5oYkITRKwmtKn6eKoO-6Y3-lI8K015fso7u-kONhhxoRr_Vv8N_gCzS332</recordid><startdate>198602</startdate><enddate>198602</enddate><creator>Nathan, R. D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198602</creationdate><title>Two electrophysiologically distinct types of cultured pacemaker cells from rabbit sinoatrial node</title><author>Nathan, R. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-d230652b627c0e13df2a5d1c2b05894faed2a56b759ddcb7254394a9174d31813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Cadmium - pharmacology</topic><topic>Cadmium Chloride</topic><topic>Electric Conductivity</topic><topic>Electrophysiology</topic><topic>Male</topic><topic>Rabbits</topic><topic>Sinoatrial Node - cytology</topic><topic>Sinoatrial Node - physiology</topic><topic>Tetrodotoxin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nathan, R. D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nathan, R. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two electrophysiologically distinct types of cultured pacemaker cells from rabbit sinoatrial node</atitle><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1986-02</date><risdate>1986</risdate><volume>250</volume><issue>2</issue><spage>H325</spage><epage>H329</epage><pages>H325-H329</pages><issn>0363-6135</issn><issn>0002-9513</issn><eissn>1522-1539</eissn><abstract>R. D. Nathan
Previous investigations employing multicellular nodal preparations (i.e.,
mixtures of dominant and subsidiary pacemaker cells) have suggested that
the fast transient inward sodium current (iNa) either is not present in
dominant pacemaker cells or is present but inactivated at the depolarized
take-off potentials that these cells exhibit. In the present study, this
question was resolved by voltage clamp analysis of single pacemaker cells
isolated from the sinoatrial node and maintained in vitro for 1-3 days. Two
types of cells, each with a different morphology, exhibited two modes of
electrophysiological behavior. Type I cells (presumably dominant
pacemakers) displayed only a tetrodotoxin (TTX)-resistant (but
cadmium-sensitive) slow inward current, whereas type II cells (presumably
subsidiary pacemakers) exhibited two components of inward current, a
TTX-sensitive, fast transient inward current and a TTX-resistant (but
cadmium-sensitive) slow inward current. Three other voltage-gated currents,
1) a slowly developing inward current activated by hyperpolarization (if,
ih, delta ip), 2) a transient outward current activated by strong
depolarization (ito, iA), and 3) a delayed outward current, were recorded
in both types of pacemaker cells.</abstract><cop>United States</cop><pmid>3946631</pmid><doi>10.1152/ajpheart.1986.250.2.h325</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6135 |
| ispartof | American journal of physiology. Heart and circulatory physiology, 1986-02, Vol.250 (2), p.H325-H329 |
| issn | 0363-6135 0002-9513 1522-1539 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpheart_250_2_H325 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Action Potentials - drug effects Animals Cadmium - pharmacology Cadmium Chloride Electric Conductivity Electrophysiology Male Rabbits Sinoatrial Node - cytology Sinoatrial Node - physiology Tetrodotoxin - pharmacology |
| title | Two electrophysiologically distinct types of cultured pacemaker cells from rabbit sinoatrial node |
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