Induction of Ca-independent PLA2 and conservation of plasmalogen polyunsaturated fatty acids in diabetic heart
1 Department of Pathology, St. Louis University Medical School, St. Louis, Missouri 63104; and Departments of 2 Pathology, 3 Pharmacology and Toxicology, and 4 Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 Diabetes-induced changes in phospholip...
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| Veröffentlicht in: | American journal of physiology: endocrinology and metabolism 2000-07, Vol.279 (1), p.E25 |
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| container_title | American journal of physiology: endocrinology and metabolism |
| container_volume | 279 |
| creator | McHowat, Jane Creer, Michael H Hicks, Kristine K Jones, Janet H McCrory, Raetreal Kennedy, Richard H |
| description | 1 Department of Pathology, St. Louis University Medical
School, St. Louis, Missouri 63104; and Departments of
2 Pathology, 3 Pharmacology and Toxicology, and
4 Pharmaceutical Sciences, University of Arkansas for Medical
Sciences, Little Rock, Arkansas 72205
Diabetes-induced changes in phospholipase A 2
(PLA 2 ) activity have been measured in several tissues but
are undefined in diabetic myocardium. We measured ventricular
PLA 2 activity in control, streptozotocin-induced diabetic,
and insulin-treated diabetic rats and characterized myocardial
phospholipids to determine whether diabetes altered myocardial
phospholipid metabolism. Increased membrane-associated
Ca 2+ -independent PLA 2 (iPLA 2 )
activity was observed in diabetes that was selective for
arachidonylated phospholipids. Increased iPLA 2 activity was
accompanied by an increase in choline lysophospholipids. Diabetes was
associated with marked alterations in the phospholipid composition of
the myocardium, characterized by decreases in esterified arachidonic
and docosahexaenoic acids and increases in linoleic acid. The decrease
in polyunsaturated fatty acids was confined to diacylphospholipids,
whereas the relative amount of these fatty acids in plasmalogens was
increased. Diabetes-induced changes in PLA 2 activity,
lysophospholipid production, and alterations in phospholipid
composition were all reversed by insulin treatment of diabetic animals.
Diabetes-induced changes in membrane phospholipid content and
phospholipid hydrolysis may contribute to some of the alterations in
myocardial function that are observed in diabetic patients.
diabetes; myocardium; phospholipase A 2 |
| doi_str_mv | 10.1152/ajpendo.2000.279.1.e25 |
| format | Article |
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School, St. Louis, Missouri 63104; and Departments of
2 Pathology, 3 Pharmacology and Toxicology, and
4 Pharmaceutical Sciences, University of Arkansas for Medical
Sciences, Little Rock, Arkansas 72205
Diabetes-induced changes in phospholipase A 2
(PLA 2 ) activity have been measured in several tissues but
are undefined in diabetic myocardium. We measured ventricular
PLA 2 activity in control, streptozotocin-induced diabetic,
and insulin-treated diabetic rats and characterized myocardial
phospholipids to determine whether diabetes altered myocardial
phospholipid metabolism. Increased membrane-associated
Ca 2+ -independent PLA 2 (iPLA 2 )
activity was observed in diabetes that was selective for
arachidonylated phospholipids. Increased iPLA 2 activity was
accompanied by an increase in choline lysophospholipids. Diabetes was
associated with marked alterations in the phospholipid composition of
the myocardium, characterized by decreases in esterified arachidonic
and docosahexaenoic acids and increases in linoleic acid. The decrease
in polyunsaturated fatty acids was confined to diacylphospholipids,
whereas the relative amount of these fatty acids in plasmalogens was
increased. Diabetes-induced changes in PLA 2 activity,
lysophospholipid production, and alterations in phospholipid
composition were all reversed by insulin treatment of diabetic animals.
Diabetes-induced changes in membrane phospholipid content and
phospholipid hydrolysis may contribute to some of the alterations in
myocardial function that are observed in diabetic patients.
diabetes; myocardium; phospholipase A 2</description><identifier>ISSN: 0193-1849</identifier><identifier>EISSN: 1522-1555</identifier><identifier>DOI: 10.1152/ajpendo.2000.279.1.e25</identifier><identifier>PMID: 10893319</identifier><language>eng</language><ispartof>American journal of physiology: endocrinology and metabolism, 2000-07, Vol.279 (1), p.E25</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>McHowat, Jane</creatorcontrib><creatorcontrib>Creer, Michael H</creatorcontrib><creatorcontrib>Hicks, Kristine K</creatorcontrib><creatorcontrib>Jones, Janet H</creatorcontrib><creatorcontrib>McCrory, Raetreal</creatorcontrib><creatorcontrib>Kennedy, Richard H</creatorcontrib><title>Induction of Ca-independent PLA2 and conservation of plasmalogen polyunsaturated fatty acids in diabetic heart</title><title>American journal of physiology: endocrinology and metabolism</title><description>1 Department of Pathology, St. Louis University Medical
School, St. Louis, Missouri 63104; and Departments of
2 Pathology, 3 Pharmacology and Toxicology, and
4 Pharmaceutical Sciences, University of Arkansas for Medical
Sciences, Little Rock, Arkansas 72205
Diabetes-induced changes in phospholipase A 2
(PLA 2 ) activity have been measured in several tissues but
are undefined in diabetic myocardium. We measured ventricular
PLA 2 activity in control, streptozotocin-induced diabetic,
and insulin-treated diabetic rats and characterized myocardial
phospholipids to determine whether diabetes altered myocardial
phospholipid metabolism. Increased membrane-associated
Ca 2+ -independent PLA 2 (iPLA 2 )
activity was observed in diabetes that was selective for
arachidonylated phospholipids. Increased iPLA 2 activity was
accompanied by an increase in choline lysophospholipids. Diabetes was
associated with marked alterations in the phospholipid composition of
the myocardium, characterized by decreases in esterified arachidonic
and docosahexaenoic acids and increases in linoleic acid. The decrease
in polyunsaturated fatty acids was confined to diacylphospholipids,
whereas the relative amount of these fatty acids in plasmalogens was
increased. Diabetes-induced changes in PLA 2 activity,
lysophospholipid production, and alterations in phospholipid
composition were all reversed by insulin treatment of diabetic animals.
Diabetes-induced changes in membrane phospholipid content and
phospholipid hydrolysis may contribute to some of the alterations in
myocardial function that are observed in diabetic patients.
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School, St. Louis, Missouri 63104; and Departments of
2 Pathology, 3 Pharmacology and Toxicology, and
4 Pharmaceutical Sciences, University of Arkansas for Medical
Sciences, Little Rock, Arkansas 72205
Diabetes-induced changes in phospholipase A 2
(PLA 2 ) activity have been measured in several tissues but
are undefined in diabetic myocardium. We measured ventricular
PLA 2 activity in control, streptozotocin-induced diabetic,
and insulin-treated diabetic rats and characterized myocardial
phospholipids to determine whether diabetes altered myocardial
phospholipid metabolism. Increased membrane-associated
Ca 2+ -independent PLA 2 (iPLA 2 )
activity was observed in diabetes that was selective for
arachidonylated phospholipids. Increased iPLA 2 activity was
accompanied by an increase in choline lysophospholipids. Diabetes was
associated with marked alterations in the phospholipid composition of
the myocardium, characterized by decreases in esterified arachidonic
and docosahexaenoic acids and increases in linoleic acid. The decrease
in polyunsaturated fatty acids was confined to diacylphospholipids,
whereas the relative amount of these fatty acids in plasmalogens was
increased. Diabetes-induced changes in PLA 2 activity,
lysophospholipid production, and alterations in phospholipid
composition were all reversed by insulin treatment of diabetic animals.
Diabetes-induced changes in membrane phospholipid content and
phospholipid hydrolysis may contribute to some of the alterations in
myocardial function that are observed in diabetic patients.
diabetes; myocardium; phospholipase A 2</abstract><pmid>10893319</pmid><doi>10.1152/ajpendo.2000.279.1.e25</doi></addata></record> |
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| identifier | ISSN: 0193-1849 |
| ispartof | American journal of physiology: endocrinology and metabolism, 2000-07, Vol.279 (1), p.E25 |
| issn | 0193-1849 1522-1555 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpendo_279_1_E25 |
| source | American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| title | Induction of Ca-independent PLA2 and conservation of plasmalogen polyunsaturated fatty acids in diabetic heart |
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