Regulation of glycogen accumulation in L6 myotubes cultured under optimized differentiation conditions
Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark The differentiation of the L6 myogenic cell line was enhanced by the addition of dexamethasone, retinoic acid, insulin-like growth factor I (IGF-I), and creatine. Spontaneous...
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Veröffentlicht in: | American journal of physiology: endocrinology and metabolism 1998-12, Vol.275 (6), p.E925-E933 |
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creator | Elsner, Peter Quistorff, Bjorn Hermann, Thomas S Dich, John Grunnet, Niels |
description | Department of Medical Biochemistry and Genetics, The Panum
Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
The differentiation of the L6 myogenic cell line
was enhanced by the addition of dexamethasone, retinoic acid,
insulin-like growth factor I (IGF-I), and creatine. Spontaneous
contractions appeared from day 10 or
11 and persisted to
day 14 or
15 . Glucose transport was increased by
insulin (100 nM) and IGF-I (5 nM) by ~60%. The highest level of
glycogen was measured in myotubes differentiated under the influence of
a combination of 5 nM dexamethasone, 100 nM retinoic acid, 5 nM IGF-I,
and 10 mM creatine with glucose as substrate. The glycogen accumulation
rate was constant from 0 to 2 h of incubation and decreased gradually
to zero at 4 h. From 0 to 0.5 h of the glycogen accumulation, the
glycogen synthase a
(GS a ) activity was 30-35% of
the total activity, with a subsequent gradual decline to 2.5% after 6 h. The glycogen phosphorylase a
(GPh a ) activity was constant at
~80% from 0 to 0.5 h, increasing to ~100% after 6 h. The activity
ratio of GS a to
GPh a decreased about sixfold without
significant change in the rate of glycogen accumulation. This indicates
that factors other than phosphorylation/dephosphorylation play a
decisive role in the regulation of glycogen metabolism in L6 myotubes.
Intracellular glucose (glucose i )
and glucose 6-phosphate (G-6- P ) may
be such factors. The observed values of these parameters may in fact
explain an activation of GS a
(G-6- P ) and an inhibition of
GPh a
(glucose i ).
muscle; cell culture; creatine kinase; glycogen metabolism; cell
differentiation |
doi_str_mv | 10.1152/ajpendo.1998.275.6.E925 |
format | Article |
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Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
The differentiation of the L6 myogenic cell line
was enhanced by the addition of dexamethasone, retinoic acid,
insulin-like growth factor I (IGF-I), and creatine. Spontaneous
contractions appeared from day 10 or
11 and persisted to
day 14 or
15 . Glucose transport was increased by
insulin (100 nM) and IGF-I (5 nM) by ~60%. The highest level of
glycogen was measured in myotubes differentiated under the influence of
a combination of 5 nM dexamethasone, 100 nM retinoic acid, 5 nM IGF-I,
and 10 mM creatine with glucose as substrate. The glycogen accumulation
rate was constant from 0 to 2 h of incubation and decreased gradually
to zero at 4 h. From 0 to 0.5 h of the glycogen accumulation, the
glycogen synthase a
(GS a ) activity was 30-35% of
the total activity, with a subsequent gradual decline to 2.5% after 6 h. The glycogen phosphorylase a
(GPh a ) activity was constant at
~80% from 0 to 0.5 h, increasing to ~100% after 6 h. The activity
ratio of GS a to
GPh a decreased about sixfold without
significant change in the rate of glycogen accumulation. This indicates
that factors other than phosphorylation/dephosphorylation play a
decisive role in the regulation of glycogen metabolism in L6 myotubes.
Intracellular glucose (glucose i )
and glucose 6-phosphate (G-6- P ) may
be such factors. The observed values of these parameters may in fact
explain an activation of GS a
(G-6- P ) and an inhibition of
GPh a
(glucose i ).
muscle; cell culture; creatine kinase; glycogen metabolism; cell
differentiation</description><identifier>ISSN: 0193-1849</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1555</identifier><identifier>DOI: 10.1152/ajpendo.1998.275.6.E925</identifier><identifier>PMID: 9843733</identifier><language>eng</language><publisher>United States</publisher><subject>Biological Transport - physiology ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Line ; Creatine - pharmacology ; Glucose - metabolism ; Glucose-6-Phosphate - metabolism ; Glycogen - metabolism ; Glycogen Synthase - metabolism ; Muscle Contraction - physiology ; Muscles - cytology ; Muscles - drug effects ; Muscles - metabolism ; Phosphorylases - metabolism</subject><ispartof>American journal of physiology: endocrinology and metabolism, 1998-12, Vol.275 (6), p.E925-E933</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-8a11a381abe3537652e21b9220ba5bce8662dd6fb91a3ca3cd3afe05b4cb083c3</citedby><cites>FETCH-LOGICAL-c450t-8a11a381abe3537652e21b9220ba5bce8662dd6fb91a3ca3cd3afe05b4cb083c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3037,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9843733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elsner, Peter</creatorcontrib><creatorcontrib>Quistorff, Bjorn</creatorcontrib><creatorcontrib>Hermann, Thomas S</creatorcontrib><creatorcontrib>Dich, John</creatorcontrib><creatorcontrib>Grunnet, Niels</creatorcontrib><title>Regulation of glycogen accumulation in L6 myotubes cultured under optimized differentiation conditions</title><title>American journal of physiology: endocrinology and metabolism</title><addtitle>Am J Physiol</addtitle><description>Department of Medical Biochemistry and Genetics, The Panum
Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
The differentiation of the L6 myogenic cell line
was enhanced by the addition of dexamethasone, retinoic acid,
insulin-like growth factor I (IGF-I), and creatine. Spontaneous
contractions appeared from day 10 or
11 and persisted to
day 14 or
15 . Glucose transport was increased by
insulin (100 nM) and IGF-I (5 nM) by ~60%. The highest level of
glycogen was measured in myotubes differentiated under the influence of
a combination of 5 nM dexamethasone, 100 nM retinoic acid, 5 nM IGF-I,
and 10 mM creatine with glucose as substrate. The glycogen accumulation
rate was constant from 0 to 2 h of incubation and decreased gradually
to zero at 4 h. From 0 to 0.5 h of the glycogen accumulation, the
glycogen synthase a
(GS a ) activity was 30-35% of
the total activity, with a subsequent gradual decline to 2.5% after 6 h. The glycogen phosphorylase a
(GPh a ) activity was constant at
~80% from 0 to 0.5 h, increasing to ~100% after 6 h. The activity
ratio of GS a to
GPh a decreased about sixfold without
significant change in the rate of glycogen accumulation. This indicates
that factors other than phosphorylation/dephosphorylation play a
decisive role in the regulation of glycogen metabolism in L6 myotubes.
Intracellular glucose (glucose i )
and glucose 6-phosphate (G-6- P ) may
be such factors. The observed values of these parameters may in fact
explain an activation of GS a
(G-6- P ) and an inhibition of
GPh a
(glucose i ).
muscle; cell culture; creatine kinase; glycogen metabolism; cell
differentiation</description><subject>Biological Transport - physiology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Creatine - pharmacology</subject><subject>Glucose - metabolism</subject><subject>Glucose-6-Phosphate - metabolism</subject><subject>Glycogen - metabolism</subject><subject>Glycogen Synthase - metabolism</subject><subject>Muscle Contraction - physiology</subject><subject>Muscles - cytology</subject><subject>Muscles - drug effects</subject><subject>Muscles - metabolism</subject><subject>Phosphorylases - metabolism</subject><issn>0193-1849</issn><issn>0002-9513</issn><issn>1522-1555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF2L1DAUhoMo6-zqTxB75V1rPpq2uZRlV4UBQdbrkCannSxpU5MG7f56M8yM640QSML7cQ4PQu8Jrgjh9KN6XGA2viJCdBVtedVUd4LyF2iXVVoSzvlLtMNEsJJ0tXiNrmN8xBi3vKZX6Ep0NWsZ26HhO4zJqdX6ufBDMbpN-xHmQmmdpotg52LfFNPm19RDLHRyawpgijQbCIVfVjvZp_w3dhggwLzaU0772djjK75BrwblIrw93zfox_3dw-2Xcv_t89fbT_tS1xyvZacIUawjqgfGWdtwCpT0glLcK95r6JqGGtMMvcg2nY9hagDM-1r3uGOa3aAPp94l-J8J4ionGzU4p2bwKcoWE8JEw7OxPRl18DEGGOQS7KTCJgmWR8LyTFgeCctMWDbySDgn351HpH4C8zd3Rpp1cdIPdjz8sgHkctii9c6Pm7xPzj3A7_XS_twrFzPkbPn_7GWhf3b5A4AYofY</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Elsner, Peter</creator><creator>Quistorff, Bjorn</creator><creator>Hermann, Thomas S</creator><creator>Dich, John</creator><creator>Grunnet, Niels</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Regulation of glycogen accumulation in L6 myotubes cultured under optimized differentiation conditions</title><author>Elsner, Peter ; Quistorff, Bjorn ; Hermann, Thomas S ; Dich, John ; Grunnet, Niels</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-8a11a381abe3537652e21b9220ba5bce8662dd6fb91a3ca3cd3afe05b4cb083c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological Transport - physiology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Creatine - pharmacology</topic><topic>Glucose - metabolism</topic><topic>Glucose-6-Phosphate - metabolism</topic><topic>Glycogen - metabolism</topic><topic>Glycogen Synthase - metabolism</topic><topic>Muscle Contraction - physiology</topic><topic>Muscles - cytology</topic><topic>Muscles - drug effects</topic><topic>Muscles - metabolism</topic><topic>Phosphorylases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elsner, Peter</creatorcontrib><creatorcontrib>Quistorff, Bjorn</creatorcontrib><creatorcontrib>Hermann, Thomas S</creatorcontrib><creatorcontrib>Dich, John</creatorcontrib><creatorcontrib>Grunnet, Niels</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elsner, Peter</au><au>Quistorff, Bjorn</au><au>Hermann, Thomas S</au><au>Dich, John</au><au>Grunnet, Niels</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of glycogen accumulation in L6 myotubes cultured under optimized differentiation conditions</atitle><jtitle>American journal of physiology: endocrinology and metabolism</jtitle><addtitle>Am J Physiol</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>275</volume><issue>6</issue><spage>E925</spage><epage>E933</epage><pages>E925-E933</pages><issn>0193-1849</issn><issn>0002-9513</issn><eissn>1522-1555</eissn><abstract>Department of Medical Biochemistry and Genetics, The Panum
Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
The differentiation of the L6 myogenic cell line
was enhanced by the addition of dexamethasone, retinoic acid,
insulin-like growth factor I (IGF-I), and creatine. Spontaneous
contractions appeared from day 10 or
11 and persisted to
day 14 or
15 . Glucose transport was increased by
insulin (100 nM) and IGF-I (5 nM) by ~60%. The highest level of
glycogen was measured in myotubes differentiated under the influence of
a combination of 5 nM dexamethasone, 100 nM retinoic acid, 5 nM IGF-I,
and 10 mM creatine with glucose as substrate. The glycogen accumulation
rate was constant from 0 to 2 h of incubation and decreased gradually
to zero at 4 h. From 0 to 0.5 h of the glycogen accumulation, the
glycogen synthase a
(GS a ) activity was 30-35% of
the total activity, with a subsequent gradual decline to 2.5% after 6 h. The glycogen phosphorylase a
(GPh a ) activity was constant at
~80% from 0 to 0.5 h, increasing to ~100% after 6 h. The activity
ratio of GS a to
GPh a decreased about sixfold without
significant change in the rate of glycogen accumulation. This indicates
that factors other than phosphorylation/dephosphorylation play a
decisive role in the regulation of glycogen metabolism in L6 myotubes.
Intracellular glucose (glucose i )
and glucose 6-phosphate (G-6- P ) may
be such factors. The observed values of these parameters may in fact
explain an activation of GS a
(G-6- P ) and an inhibition of
GPh a
(glucose i ).
muscle; cell culture; creatine kinase; glycogen metabolism; cell
differentiation</abstract><cop>United States</cop><pmid>9843733</pmid><doi>10.1152/ajpendo.1998.275.6.E925</doi></addata></record> |
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source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
subjects | Biological Transport - physiology Cell Differentiation - drug effects Cell Differentiation - physiology Cell Line Creatine - pharmacology Glucose - metabolism Glucose-6-Phosphate - metabolism Glycogen - metabolism Glycogen Synthase - metabolism Muscle Contraction - physiology Muscles - cytology Muscles - drug effects Muscles - metabolism Phosphorylases - metabolism |
title | Regulation of glycogen accumulation in L6 myotubes cultured under optimized differentiation conditions |
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