Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids
Stearoyl-CoA desaturase (SCD) is a key regulatory enzyme in the synthesis of unsaturated fatty acids. Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We add...
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| Veröffentlicht in: | American journal of physiology: endocrinology and metabolism 1996-07, Vol.271 (1), p.E44-E49 |
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| container_issue | 1 |
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| container_title | American journal of physiology: endocrinology and metabolism |
| container_volume | 271 |
| creator | Jones, B.H Maher, M.A Banz, W.J Zemel, M.B Whelan, J Smith, P.J Moustaid, N |
| description | Stearoyl-CoA desaturase (SCD) is a key regulatory enzyme in the synthesis of unsaturated fatty acids. Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We addressed these questions by feeding control (12% corn oil) and high-PUFA (48% corn oil) diets to lean and obese Zucker rats and analyzing SCD mRNA levels in adipose tissue and liver. We report that SCD mRNA content was dramatically elevated in adipose tissue of obese vs. lean rats on both diets and was significantly decreased by PUFA in both genotypes. Interestingly, we demonstrate that SCD expression was directly downregulated in a dose-dependent manner by PUFA in 3T3-L1 adipocytes. We conclude that 1) obese Zucker rats overexpress the SCD gene in both liver and adipose tissue and 2) PUFA directly suppress SCD expression in adipocytes. Further studies will elucidate the mechanisms responsible for obesity- and PUFA-mediated regulation of SCD in adipose cells. |
| doi_str_mv | 10.1152/ajpendo.1996.271.1.e44 |
| format | Article |
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Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We addressed these questions by feeding control (12% corn oil) and high-PUFA (48% corn oil) diets to lean and obese Zucker rats and analyzing SCD mRNA levels in adipose tissue and liver. We report that SCD mRNA content was dramatically elevated in adipose tissue of obese vs. lean rats on both diets and was significantly decreased by PUFA in both genotypes. Interestingly, we demonstrate that SCD expression was directly downregulated in a dose-dependent manner by PUFA in 3T3-L1 adipocytes. We conclude that 1) obese Zucker rats overexpress the SCD gene in both liver and adipose tissue and 2) PUFA directly suppress SCD expression in adipocytes. Further studies will elucidate the mechanisms responsible for obesity- and PUFA-mediated regulation of SCD in adipose cells.</description><identifier>ISSN: 0002-9513</identifier><identifier>ISSN: 0193-1849</identifier><identifier>EISSN: 2163-5773</identifier><identifier>EISSN: 1522-1555</identifier><identifier>DOI: 10.1152/ajpendo.1996.271.1.e44</identifier><identifier>PMID: 8760080</identifier><language>eng</language><publisher>United States</publisher><subject>Adipocytes - metabolism ; Adipose Tissue - metabolism ; Adipose Tissue - pathology ; Animals ; Cell Line ; diet-related diseases ; Fatty Acids - chemistry ; Fatty Acids - metabolism ; Fatty Acids, Unsaturated - pharmacology ; human nutrition ; Liver - metabolism ; Male ; Obesity - metabolism ; Obesity - pathology ; Rats ; Rats, Zucker ; Reference Values ; RNA, Messenger - metabolism ; Stearoyl-CoA Desaturase - genetics</subject><ispartof>American journal of physiology: endocrinology and metabolism, 1996-07, Vol.271 (1), p.E44-E49</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-8d0367e23bcf4235d53a6b7facf8ffc96f0cd7fd2d449a23df3831d9f76abb073</citedby><cites>FETCH-LOGICAL-c367t-8d0367e23bcf4235d53a6b7facf8ffc96f0cd7fd2d449a23df3831d9f76abb073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8760080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, B.H</creatorcontrib><creatorcontrib>Maher, M.A</creatorcontrib><creatorcontrib>Banz, W.J</creatorcontrib><creatorcontrib>Zemel, M.B</creatorcontrib><creatorcontrib>Whelan, J</creatorcontrib><creatorcontrib>Smith, P.J</creatorcontrib><creatorcontrib>Moustaid, N</creatorcontrib><title>Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids</title><title>American journal of physiology: endocrinology and metabolism</title><addtitle>Am J Physiol</addtitle><description>Stearoyl-CoA desaturase (SCD) is a key regulatory enzyme in the synthesis of unsaturated fatty acids. Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We addressed these questions by feeding control (12% corn oil) and high-PUFA (48% corn oil) diets to lean and obese Zucker rats and analyzing SCD mRNA levels in adipose tissue and liver. We report that SCD mRNA content was dramatically elevated in adipose tissue of obese vs. lean rats on both diets and was significantly decreased by PUFA in both genotypes. Interestingly, we demonstrate that SCD expression was directly downregulated in a dose-dependent manner by PUFA in 3T3-L1 adipocytes. We conclude that 1) obese Zucker rats overexpress the SCD gene in both liver and adipose tissue and 2) PUFA directly suppress SCD expression in adipocytes. Further studies will elucidate the mechanisms responsible for obesity- and PUFA-mediated regulation of SCD in adipose cells.</description><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - metabolism</subject><subject>Adipose Tissue - pathology</subject><subject>Animals</subject><subject>Cell Line</subject><subject>diet-related diseases</subject><subject>Fatty Acids - chemistry</subject><subject>Fatty Acids - metabolism</subject><subject>Fatty Acids, Unsaturated - pharmacology</subject><subject>human nutrition</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Obesity - metabolism</subject><subject>Obesity - pathology</subject><subject>Rats</subject><subject>Rats, Zucker</subject><subject>Reference Values</subject><subject>RNA, Messenger - metabolism</subject><subject>Stearoyl-CoA Desaturase - genetics</subject><issn>0002-9513</issn><issn>0193-1849</issn><issn>2163-5773</issn><issn>1522-1555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkVtL5DAUx8OiuKO7H0G3Tz5ta25t2sdh0HVBVtD1OaS5zEQ6Tc1pkX57M3QQnw45_8uBXxC6IrggpKQ36nWwvQkFaZqqoIIUpLCcf0MrSiqWl0KwE7TCGNO8KQn7js4BXtOTlIyfobNaVBjXeIWGtfFDAJuNHmCyGYxWxTB3-SasM2NBjVNUSd4__VtnHjLf62jTwmTtnIXWgh_nTPUmeb8IQ-jmqV_CY1o5NR5s2hv4gU6d6sD-PM4L9HJ3-39znz88_vm7WT_kmlVizGuD07SUtdpxykpTMlW1wintaud0UzmsjXCGGs4bRZlxrGbENE5Uqm2xYBfoeukdYnibLIxy70HbrlO9DRNIUVNSN-JgrBajjgEgWieH6PcqzpJgeUAtj6jlAbVMqCWRt5yn4OXxwtTurfmMHdkm_fei7_x29-6jlcNuBh-6sJ0_O7_W_VrsTgWpttGDfHmmmLD0Z7RmjLMPXSWYUw</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Jones, B.H</creator><creator>Maher, M.A</creator><creator>Banz, W.J</creator><creator>Zemel, M.B</creator><creator>Whelan, J</creator><creator>Smith, P.J</creator><creator>Moustaid, N</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960701</creationdate><title>Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids</title><author>Jones, B.H ; Maher, M.A ; Banz, W.J ; Zemel, M.B ; Whelan, J ; Smith, P.J ; Moustaid, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-8d0367e23bcf4235d53a6b7facf8ffc96f0cd7fd2d449a23df3831d9f76abb073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adipocytes - metabolism</topic><topic>Adipose Tissue - metabolism</topic><topic>Adipose Tissue - pathology</topic><topic>Animals</topic><topic>Cell Line</topic><topic>diet-related diseases</topic><topic>Fatty Acids - chemistry</topic><topic>Fatty Acids - metabolism</topic><topic>Fatty Acids, Unsaturated - pharmacology</topic><topic>human nutrition</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Obesity - metabolism</topic><topic>Obesity - pathology</topic><topic>Rats</topic><topic>Rats, Zucker</topic><topic>Reference Values</topic><topic>RNA, Messenger - metabolism</topic><topic>Stearoyl-CoA Desaturase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, B.H</creatorcontrib><creatorcontrib>Maher, M.A</creatorcontrib><creatorcontrib>Banz, W.J</creatorcontrib><creatorcontrib>Zemel, M.B</creatorcontrib><creatorcontrib>Whelan, J</creatorcontrib><creatorcontrib>Smith, P.J</creatorcontrib><creatorcontrib>Moustaid, N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, B.H</au><au>Maher, M.A</au><au>Banz, W.J</au><au>Zemel, M.B</au><au>Whelan, J</au><au>Smith, P.J</au><au>Moustaid, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids</atitle><jtitle>American journal of physiology: endocrinology and metabolism</jtitle><addtitle>Am J Physiol</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>271</volume><issue>1</issue><spage>E44</spage><epage>E49</epage><pages>E44-E49</pages><issn>0002-9513</issn><issn>0193-1849</issn><eissn>2163-5773</eissn><eissn>1522-1555</eissn><abstract>Stearoyl-CoA desaturase (SCD) is a key regulatory enzyme in the synthesis of unsaturated fatty acids. Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We addressed these questions by feeding control (12% corn oil) and high-PUFA (48% corn oil) diets to lean and obese Zucker rats and analyzing SCD mRNA levels in adipose tissue and liver. We report that SCD mRNA content was dramatically elevated in adipose tissue of obese vs. lean rats on both diets and was significantly decreased by PUFA in both genotypes. Interestingly, we demonstrate that SCD expression was directly downregulated in a dose-dependent manner by PUFA in 3T3-L1 adipocytes. We conclude that 1) obese Zucker rats overexpress the SCD gene in both liver and adipose tissue and 2) PUFA directly suppress SCD expression in adipocytes. Further studies will elucidate the mechanisms responsible for obesity- and PUFA-mediated regulation of SCD in adipose cells.</abstract><cop>United States</cop><pmid>8760080</pmid><doi>10.1152/ajpendo.1996.271.1.e44</doi></addata></record> |
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| ispartof | American journal of physiology: endocrinology and metabolism, 1996-07, Vol.271 (1), p.E44-E49 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adipocytes - metabolism Adipose Tissue - metabolism Adipose Tissue - pathology Animals Cell Line diet-related diseases Fatty Acids - chemistry Fatty Acids - metabolism Fatty Acids, Unsaturated - pharmacology human nutrition Liver - metabolism Male Obesity - metabolism Obesity - pathology Rats Rats, Zucker Reference Values RNA, Messenger - metabolism Stearoyl-CoA Desaturase - genetics |
| title | Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids |
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