Production of glutamine and utilization of glutamate by rat subcutaneous adipose tissue in vivo
T. J. Kowalski and M. Watford Department of Nutritional Sciences, Cook College, Rutgers University, New Brunswick, New Jersey 08903. Information about adipose tissue amino acid metabolism is limited, with most data derived from studies in vitro. The purpose of this study was to further characterize...
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| Veröffentlicht in: | American journal of physiology: endocrinology and metabolism 1994-01, Vol.266 (1), p.E151-E154 |
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| container_title | American journal of physiology: endocrinology and metabolism |
| container_volume | 266 |
| creator | Kowalski, T. J Watford, M |
| description | T. J. Kowalski and M. Watford
Department of Nutritional Sciences, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Information about adipose tissue amino acid metabolism is limited, with
most data derived from studies in vitro. The purpose of this study was to
further characterize the role of adipose tissue in glutamine metabolism in
the rat in vivo. The extracellular concentrations of glutamine, glutamate,
alanine, and ammonia were measured in the rat inguinal fat pad using a
microdialysis sampling technique. A calibration method was used to
accurately assess the extracellular levels of metabolites, and a comparison
of these concentrations with those in arterial blood allowed determination
of the net flux of each compound. The adipose tissue-arterial blood
concentration differences were 122 +/- 19, 54 +/- 37, -61 +/- 21, and -28
+/- 13 microM for glutamine, alanine, glutamate, and ammonia, respectively,
indicating a production of glutamine and an uptake of glutamate by
subcutaneous adipose tissue. The magnitude of glutamine production suggests
that adipose tissue may play a significant role in whole body glutamine
homeostasis. |
| doi_str_mv | 10.1152/ajpendo.1994.266.1.e151 |
| format | Article |
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Department of Nutritional Sciences, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Information about adipose tissue amino acid metabolism is limited, with
most data derived from studies in vitro. The purpose of this study was to
further characterize the role of adipose tissue in glutamine metabolism in
the rat in vivo. The extracellular concentrations of glutamine, glutamate,
alanine, and ammonia were measured in the rat inguinal fat pad using a
microdialysis sampling technique. A calibration method was used to
accurately assess the extracellular levels of metabolites, and a comparison
of these concentrations with those in arterial blood allowed determination
of the net flux of each compound. The adipose tissue-arterial blood
concentration differences were 122 +/- 19, 54 +/- 37, -61 +/- 21, and -28
+/- 13 microM for glutamine, alanine, glutamate, and ammonia, respectively,
indicating a production of glutamine and an uptake of glutamate by
subcutaneous adipose tissue. The magnitude of glutamine production suggests
that adipose tissue may play a significant role in whole body glutamine
homeostasis.</description><identifier>ISSN: 0193-1849</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1555</identifier><identifier>DOI: 10.1152/ajpendo.1994.266.1.e151</identifier><identifier>PMID: 7905708</identifier><language>eng</language><publisher>United States</publisher><subject>Adipose Tissue - metabolism ; Animals ; Calibration ; Glutamates - metabolism ; Glutamic Acid ; Glutamine - biosynthesis ; In Vitro Techniques ; Male ; Microdialysis ; Rats ; Rats, Sprague-Dawley ; Regression Analysis ; Skin</subject><ispartof>American journal of physiology: endocrinology and metabolism, 1994-01, Vol.266 (1), p.E151-E154</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-5d11e5857a9f49401ebcd05aecefeccffc389d0ffc955160dc25ccfee85267373</citedby><cites>FETCH-LOGICAL-c378t-5d11e5857a9f49401ebcd05aecefeccffc389d0ffc955160dc25ccfee85267373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7905708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kowalski, T. J</creatorcontrib><creatorcontrib>Watford, M</creatorcontrib><title>Production of glutamine and utilization of glutamate by rat subcutaneous adipose tissue in vivo</title><title>American journal of physiology: endocrinology and metabolism</title><addtitle>Am J Physiol</addtitle><description>T. J. Kowalski and M. Watford
Department of Nutritional Sciences, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Information about adipose tissue amino acid metabolism is limited, with
most data derived from studies in vitro. The purpose of this study was to
further characterize the role of adipose tissue in glutamine metabolism in
the rat in vivo. The extracellular concentrations of glutamine, glutamate,
alanine, and ammonia were measured in the rat inguinal fat pad using a
microdialysis sampling technique. A calibration method was used to
accurately assess the extracellular levels of metabolites, and a comparison
of these concentrations with those in arterial blood allowed determination
of the net flux of each compound. The adipose tissue-arterial blood
concentration differences were 122 +/- 19, 54 +/- 37, -61 +/- 21, and -28
+/- 13 microM for glutamine, alanine, glutamate, and ammonia, respectively,
indicating a production of glutamine and an uptake of glutamate by
subcutaneous adipose tissue. The magnitude of glutamine production suggests
that adipose tissue may play a significant role in whole body glutamine
homeostasis.</description><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Calibration</subject><subject>Glutamates - metabolism</subject><subject>Glutamic Acid</subject><subject>Glutamine - biosynthesis</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Microdialysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regression Analysis</subject><subject>Skin</subject><issn>0193-1849</issn><issn>0002-9513</issn><issn>1522-1555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1OwzAQhC0EKqXwCAifuCXYSRzHR1SVH6kSHOBsOfamdZXGIU6KytPjqlEFp5F2ZmdXH0J3lMSUsuRBbVpojIupEFmc5HlMY6CMnqFpcJOIMsbO0ZRQkUa0yMQluvJ-QwjhLEsmaMIFYZwUUyTfO2cG3VvXYFfhVT30amsbwKoxeOhtbX_Uf1P1gMs97lSP_VDqMGrADR4rY1vnAffW-wGwbfDO7tw1uqhU7eFm1Bn6fFp8zF-i5dvz6_xxGemUF33EDKXACsaVqDKREQqlNoQp0FCB1lWl00IYElQwRnNidMLCGKBgSc5Tns7Q_bG37dzXAL6XW-s11PXxO8nzNCsIS0OQH4O6c953UMm2s1vV7SUl8oBWjmjlAa0MaCWVi4A2bN6OJ4ZyC-a0N7IMfnT013a1_rYdyHa999bVbrU_lf7p-wW0PYp9</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Kowalski, T. J</creator><creator>Watford, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940101</creationdate><title>Production of glutamine and utilization of glutamate by rat subcutaneous adipose tissue in vivo</title><author>Kowalski, T. J ; Watford, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-5d11e5857a9f49401ebcd05aecefeccffc389d0ffc955160dc25ccfee85267373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>Calibration</topic><topic>Glutamates - metabolism</topic><topic>Glutamic Acid</topic><topic>Glutamine - biosynthesis</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Microdialysis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regression Analysis</topic><topic>Skin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kowalski, T. J</creatorcontrib><creatorcontrib>Watford, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kowalski, T. J</au><au>Watford, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of glutamine and utilization of glutamate by rat subcutaneous adipose tissue in vivo</atitle><jtitle>American journal of physiology: endocrinology and metabolism</jtitle><addtitle>Am J Physiol</addtitle><date>1994-01-01</date><risdate>1994</risdate><volume>266</volume><issue>1</issue><spage>E151</spage><epage>E154</epage><pages>E151-E154</pages><issn>0193-1849</issn><issn>0002-9513</issn><eissn>1522-1555</eissn><abstract>T. J. Kowalski and M. Watford
Department of Nutritional Sciences, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Information about adipose tissue amino acid metabolism is limited, with
most data derived from studies in vitro. The purpose of this study was to
further characterize the role of adipose tissue in glutamine metabolism in
the rat in vivo. The extracellular concentrations of glutamine, glutamate,
alanine, and ammonia were measured in the rat inguinal fat pad using a
microdialysis sampling technique. A calibration method was used to
accurately assess the extracellular levels of metabolites, and a comparison
of these concentrations with those in arterial blood allowed determination
of the net flux of each compound. The adipose tissue-arterial blood
concentration differences were 122 +/- 19, 54 +/- 37, -61 +/- 21, and -28
+/- 13 microM for glutamine, alanine, glutamate, and ammonia, respectively,
indicating a production of glutamine and an uptake of glutamate by
subcutaneous adipose tissue. The magnitude of glutamine production suggests
that adipose tissue may play a significant role in whole body glutamine
homeostasis.</abstract><cop>United States</cop><pmid>7905708</pmid><doi>10.1152/ajpendo.1994.266.1.e151</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0193-1849 |
| ispartof | American journal of physiology: endocrinology and metabolism, 1994-01, Vol.266 (1), p.E151-E154 |
| issn | 0193-1849 0002-9513 1522-1555 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpendo_266_1_E151 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adipose Tissue - metabolism Animals Calibration Glutamates - metabolism Glutamic Acid Glutamine - biosynthesis In Vitro Techniques Male Microdialysis Rats Rats, Sprague-Dawley Regression Analysis Skin |
| title | Production of glutamine and utilization of glutamate by rat subcutaneous adipose tissue in vivo |
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