Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2
H. F. DeLuca, R. R. Sicinski, Y. Tanaka, P. H. Stern and C. M. Smith Department of Biochemistry, University of Wisconsin-Madison 53706. The biological activity of 1,25-dihydroxyvitamin D2 [1,25(OH)2D2] and 24-epi-1,25-dihydroxyvitamin D2 [24-epi-1,25(OH)2D2] has been determined in vitamin D-deficien...
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| Veröffentlicht in: | American journal of physiology: endocrinology and metabolism 1988-04, Vol.254 (4), p.E402-E406 |
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| container_title | American journal of physiology: endocrinology and metabolism |
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| creator | DeLuca, H.F Sicinski, R.R Tanaka, Y Stern, P.H Smith, C.M |
| description | H. F. DeLuca, R. R. Sicinski, Y. Tanaka, P. H. Stern and C. M. Smith
Department of Biochemistry, University of Wisconsin-Madison 53706.
The biological activity of 1,25-dihydroxyvitamin D2 [1,25(OH)2D2] and
24-epi-1,25-dihydroxyvitamin D2 [24-epi-1,25(OH)2D2] has been determined in
vitamin D-deficient rats. The biological effectiveness of 1,25(OH)2D2 is
equal to that reported previously for 1,25-dihydroxyvitamin D3
[1,25(OH)2D3] (15) in intestinal calcium transport, mineralization of bone,
mobilization of bone calcium, and elevation of plasma inorganic phosphorus
of rachitic rats. However, 24-epi-1,25(OH)2D2 is at best one-half as active
as 1,25(OH)2D2 in stimulating intestinal calcium transport and in the
mineralization of rachitic bone. The 24-epi-1,25(OH)2D2 is one-third as
active as 1,25(OH)2D3 in binding to the chick intestinal receptor for
1,25(OH)2D3. Thus receptor discrimination may account for the twofold
difference in intestinal calcium transport activity. 24-Epi-1,25(OH)2D2
appeared inactive in in vivo mobilization of bone calcium or bone
phosphorus. On the other hand, in fetal rat bone in culture, the epi
compound was only five times less active than 1,25(OH)2D2 in inducing
resorption. Short-term experiments on bone mineral mobilization in vivo
show that the 24-epi-1,25(OH)2D2 does induce bone calcium mobilization but
that its activity in this respect is short lived. It is suggested that
24-epi-1,25(OH)2D2 and, as a result, it shows preferential activity on
intestine whose response to a single dose of 1,25(OH)2D2 remains for
several days, whereas the short-lived bone system does not remain
stimulated during the 24-h period between doses. |
| doi_str_mv | 10.1152/ajpendo.1988.254.4.E402 |
| format | Article |
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Department of Biochemistry, University of Wisconsin-Madison 53706.
The biological activity of 1,25-dihydroxyvitamin D2 [1,25(OH)2D2] and
24-epi-1,25-dihydroxyvitamin D2 [24-epi-1,25(OH)2D2] has been determined in
vitamin D-deficient rats. The biological effectiveness of 1,25(OH)2D2 is
equal to that reported previously for 1,25-dihydroxyvitamin D3
[1,25(OH)2D3] (15) in intestinal calcium transport, mineralization of bone,
mobilization of bone calcium, and elevation of plasma inorganic phosphorus
of rachitic rats. However, 24-epi-1,25(OH)2D2 is at best one-half as active
as 1,25(OH)2D2 in stimulating intestinal calcium transport and in the
mineralization of rachitic bone. The 24-epi-1,25(OH)2D2 is one-third as
active as 1,25(OH)2D3 in binding to the chick intestinal receptor for
1,25(OH)2D3. Thus receptor discrimination may account for the twofold
difference in intestinal calcium transport activity. 24-Epi-1,25(OH)2D2
appeared inactive in in vivo mobilization of bone calcium or bone
phosphorus. On the other hand, in fetal rat bone in culture, the epi
compound was only five times less active than 1,25(OH)2D2 in inducing
resorption. Short-term experiments on bone mineral mobilization in vivo
show that the 24-epi-1,25(OH)2D2 does induce bone calcium mobilization but
that its activity in this respect is short lived. It is suggested that
24-epi-1,25(OH)2D2 and, as a result, it shows preferential activity on
intestine whose response to a single dose of 1,25(OH)2D2 remains for
several days, whereas the short-lived bone system does not remain
stimulated during the 24-h period between doses.</description><identifier>ISSN: 0002-9513</identifier><identifier>ISSN: 0193-1849</identifier><identifier>EISSN: 2163-5773</identifier><identifier>EISSN: 1522-1555</identifier><identifier>DOI: 10.1152/ajpendo.1988.254.4.E402</identifier><identifier>PMID: 2833108</identifier><language>eng</language><publisher>United States</publisher><subject>ABSORCION ; ABSORPTION ; Animals ; BONE DISEASES ; Bone Resorption - drug effects ; CALCIO ; Calcitriol - pharmacology ; CALCIUM ; Calcium - metabolism ; Chickens ; ENFERMEDADES OSEAS ; Ergocalciferols - analogs & derivatives ; Ergocalciferols - metabolism ; Ergocalciferols - pharmacology ; Intestinal Absorption - drug effects ; Intestines - metabolism ; Kinetics ; Male ; NUTRITIVE VALUE ; OSTEOPATHIE ; Rats ; Receptors, Calcitriol ; Receptors, Steroid - metabolism ; Reference Values ; Space life sciences ; VALEUR NUTRITIVE ; VALOR NUTRITIVO ; VITAMIN D ; Vitamin D Deficiency - metabolism ; VITAMINA D ; VITAMINE D</subject><ispartof>American journal of physiology: endocrinology and metabolism, 1988-04, Vol.254 (4), p.E402-E406</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c243t-a0bfdb1e4cc73c2122534e626022d31e840130cfbbcb75fe65cc77f2751bc98d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2833108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DeLuca, H.F</creatorcontrib><creatorcontrib>Sicinski, R.R</creatorcontrib><creatorcontrib>Tanaka, Y</creatorcontrib><creatorcontrib>Stern, P.H</creatorcontrib><creatorcontrib>Smith, C.M</creatorcontrib><title>Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2</title><title>American journal of physiology: endocrinology and metabolism</title><addtitle>Am J Physiol</addtitle><description>H. F. DeLuca, R. R. Sicinski, Y. Tanaka, P. H. Stern and C. M. Smith
Department of Biochemistry, University of Wisconsin-Madison 53706.
The biological activity of 1,25-dihydroxyvitamin D2 [1,25(OH)2D2] and
24-epi-1,25-dihydroxyvitamin D2 [24-epi-1,25(OH)2D2] has been determined in
vitamin D-deficient rats. The biological effectiveness of 1,25(OH)2D2 is
equal to that reported previously for 1,25-dihydroxyvitamin D3
[1,25(OH)2D3] (15) in intestinal calcium transport, mineralization of bone,
mobilization of bone calcium, and elevation of plasma inorganic phosphorus
of rachitic rats. However, 24-epi-1,25(OH)2D2 is at best one-half as active
as 1,25(OH)2D2 in stimulating intestinal calcium transport and in the
mineralization of rachitic bone. The 24-epi-1,25(OH)2D2 is one-third as
active as 1,25(OH)2D3 in binding to the chick intestinal receptor for
1,25(OH)2D3. Thus receptor discrimination may account for the twofold
difference in intestinal calcium transport activity. 24-Epi-1,25(OH)2D2
appeared inactive in in vivo mobilization of bone calcium or bone
phosphorus. On the other hand, in fetal rat bone in culture, the epi
compound was only five times less active than 1,25(OH)2D2 in inducing
resorption. Short-term experiments on bone mineral mobilization in vivo
show that the 24-epi-1,25(OH)2D2 does induce bone calcium mobilization but
that its activity in this respect is short lived. It is suggested that
24-epi-1,25(OH)2D2 and, as a result, it shows preferential activity on
intestine whose response to a single dose of 1,25(OH)2D2 remains for
several days, whereas the short-lived bone system does not remain
stimulated during the 24-h period between doses.</description><subject>ABSORCION</subject><subject>ABSORPTION</subject><subject>Animals</subject><subject>BONE DISEASES</subject><subject>Bone Resorption - drug effects</subject><subject>CALCIO</subject><subject>Calcitriol - pharmacology</subject><subject>CALCIUM</subject><subject>Calcium - metabolism</subject><subject>Chickens</subject><subject>ENFERMEDADES OSEAS</subject><subject>Ergocalciferols - analogs & derivatives</subject><subject>Ergocalciferols - metabolism</subject><subject>Ergocalciferols - pharmacology</subject><subject>Intestinal Absorption - drug effects</subject><subject>Intestines - metabolism</subject><subject>Kinetics</subject><subject>Male</subject><subject>NUTRITIVE VALUE</subject><subject>OSTEOPATHIE</subject><subject>Rats</subject><subject>Receptors, Calcitriol</subject><subject>Receptors, Steroid - metabolism</subject><subject>Reference Values</subject><subject>Space life sciences</subject><subject>VALEUR NUTRITIVE</subject><subject>VALOR NUTRITIVO</subject><subject>VITAMIN D</subject><subject>Vitamin D Deficiency - metabolism</subject><subject>VITAMINA D</subject><subject>VITAMINE D</subject><issn>0002-9513</issn><issn>0193-1849</issn><issn>2163-5773</issn><issn>1522-1555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1P4zAQhi20CMrHH0BC5MRpHTxjO3aPC8uXVIlD4Ww5jtMapXWIW9j8-01phcSBkzWeZ94ZPYRcAMsBJF7Z19Yvq5jDWOscpchFfisY7pERQsGpVIr_IiPGGNKxBH5IjlJ6HUqQXByQA9ScA9MjMr0OsYmz4GyTWbcK72HVZ7HO4DdKWoV5X3XxXz_82kVYZn8xs8sqQ0F9G-hPzAnZr22T_OnuPSYvd7fPNw908nT_ePNnQh0KvqKWlXVVghfOKe4QEIfbfIEFQ6w4eC0YcObqsnSlkrUv5ACqGpWE0o11xY_J5Ta37eLb2qeVWYTkfNPYpY_rZJSGAoDJAVRb0HUxpc7Xpu3Cwna9AWY2Os1Op9noNINOI8xG5zB5vluxLhe--prb-Rv6dNufh9n8I3TetPM-fSrtv0K_5Z1t-dpGY2ddSOZlqjVXfCz4f5dSidQ</recordid><startdate>198804</startdate><enddate>198804</enddate><creator>DeLuca, H.F</creator><creator>Sicinski, R.R</creator><creator>Tanaka, Y</creator><creator>Stern, P.H</creator><creator>Smith, C.M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198804</creationdate><title>Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2</title><author>DeLuca, H.F ; Sicinski, R.R ; Tanaka, Y ; Stern, P.H ; Smith, C.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c243t-a0bfdb1e4cc73c2122534e626022d31e840130cfbbcb75fe65cc77f2751bc98d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>ABSORCION</topic><topic>ABSORPTION</topic><topic>Animals</topic><topic>BONE DISEASES</topic><topic>Bone Resorption - drug effects</topic><topic>CALCIO</topic><topic>Calcitriol - pharmacology</topic><topic>CALCIUM</topic><topic>Calcium - metabolism</topic><topic>Chickens</topic><topic>ENFERMEDADES OSEAS</topic><topic>Ergocalciferols - analogs & derivatives</topic><topic>Ergocalciferols - metabolism</topic><topic>Ergocalciferols - pharmacology</topic><topic>Intestinal Absorption - drug effects</topic><topic>Intestines - metabolism</topic><topic>Kinetics</topic><topic>Male</topic><topic>NUTRITIVE VALUE</topic><topic>OSTEOPATHIE</topic><topic>Rats</topic><topic>Receptors, Calcitriol</topic><topic>Receptors, Steroid - metabolism</topic><topic>Reference Values</topic><topic>Space life sciences</topic><topic>VALEUR NUTRITIVE</topic><topic>VALOR NUTRITIVO</topic><topic>VITAMIN D</topic><topic>Vitamin D Deficiency - metabolism</topic><topic>VITAMINA D</topic><topic>VITAMINE D</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DeLuca, H.F</creatorcontrib><creatorcontrib>Sicinski, R.R</creatorcontrib><creatorcontrib>Tanaka, Y</creatorcontrib><creatorcontrib>Stern, P.H</creatorcontrib><creatorcontrib>Smith, C.M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DeLuca, H.F</au><au>Sicinski, R.R</au><au>Tanaka, Y</au><au>Stern, P.H</au><au>Smith, C.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2</atitle><jtitle>American journal of physiology: endocrinology and metabolism</jtitle><addtitle>Am J Physiol</addtitle><date>1988-04</date><risdate>1988</risdate><volume>254</volume><issue>4</issue><spage>E402</spage><epage>E406</epage><pages>E402-E406</pages><issn>0002-9513</issn><issn>0193-1849</issn><eissn>2163-5773</eissn><eissn>1522-1555</eissn><abstract>H. F. DeLuca, R. R. Sicinski, Y. Tanaka, P. H. Stern and C. M. Smith
Department of Biochemistry, University of Wisconsin-Madison 53706.
The biological activity of 1,25-dihydroxyvitamin D2 [1,25(OH)2D2] and
24-epi-1,25-dihydroxyvitamin D2 [24-epi-1,25(OH)2D2] has been determined in
vitamin D-deficient rats. The biological effectiveness of 1,25(OH)2D2 is
equal to that reported previously for 1,25-dihydroxyvitamin D3
[1,25(OH)2D3] (15) in intestinal calcium transport, mineralization of bone,
mobilization of bone calcium, and elevation of plasma inorganic phosphorus
of rachitic rats. However, 24-epi-1,25(OH)2D2 is at best one-half as active
as 1,25(OH)2D2 in stimulating intestinal calcium transport and in the
mineralization of rachitic bone. The 24-epi-1,25(OH)2D2 is one-third as
active as 1,25(OH)2D3 in binding to the chick intestinal receptor for
1,25(OH)2D3. Thus receptor discrimination may account for the twofold
difference in intestinal calcium transport activity. 24-Epi-1,25(OH)2D2
appeared inactive in in vivo mobilization of bone calcium or bone
phosphorus. On the other hand, in fetal rat bone in culture, the epi
compound was only five times less active than 1,25(OH)2D2 in inducing
resorption. Short-term experiments on bone mineral mobilization in vivo
show that the 24-epi-1,25(OH)2D2 does induce bone calcium mobilization but
that its activity in this respect is short lived. It is suggested that
24-epi-1,25(OH)2D2 and, as a result, it shows preferential activity on
intestine whose response to a single dose of 1,25(OH)2D2 remains for
several days, whereas the short-lived bone system does not remain
stimulated during the 24-h period between doses.</abstract><cop>United States</cop><pmid>2833108</pmid><doi>10.1152/ajpendo.1988.254.4.E402</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9513 |
| ispartof | American journal of physiology: endocrinology and metabolism, 1988-04, Vol.254 (4), p.E402-E406 |
| issn | 0002-9513 0193-1849 2163-5773 1522-1555 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpendo_254_4_E402 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | ABSORCION ABSORPTION Animals BONE DISEASES Bone Resorption - drug effects CALCIO Calcitriol - pharmacology CALCIUM Calcium - metabolism Chickens ENFERMEDADES OSEAS Ergocalciferols - analogs & derivatives Ergocalciferols - metabolism Ergocalciferols - pharmacology Intestinal Absorption - drug effects Intestines - metabolism Kinetics Male NUTRITIVE VALUE OSTEOPATHIE Rats Receptors, Calcitriol Receptors, Steroid - metabolism Reference Values Space life sciences VALEUR NUTRITIVE VALOR NUTRITIVO VITAMIN D Vitamin D Deficiency - metabolism VITAMINA D VITAMINE D |
| title | Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2 |
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