Extracellular Na+ inhibits Na+/H+ exchange: cell shrinkage reduces the inhibition
Department of Biology, Syracuse University, Syracuse, New York 13244-1220 Submitted 30 December 2003 ; accepted in final form 18 March 2004 Na + /H + exchangers (NHE) are ubiquitous transporters participating in regulation of cell volume and pH. Cell shrinkage, acidification, and growth factors acti...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 2004-08, Vol.287 (2), p.C336-C344 |
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| container_title | American Journal of Physiology: Cell Physiology |
| container_volume | 287 |
| creator | Dunham, Philip B Kelley, Scott J Logue, Paul J |
| description | Department of Biology, Syracuse University, Syracuse, New York 13244-1220
Submitted 30 December 2003
; accepted in final form 18 March 2004
Na + /H + exchangers (NHE) are ubiquitous transporters participating in regulation of cell volume and pH. Cell shrinkage, acidification, and growth factors activate NHE by increasing its sensitivity to intracellular H + concentration. In this study, the kinetics were studied in dog red blood cells of Na + influx through NHE as a function of external Na + concentration ([Na + ] o ). In cells in isotonic media, [Na + ] o inhibited Na + influx >40 mM. Osmotic shrinkage activated NHE by reducing this inhibition. In cells in isotonic media + 120 mM sucrose, there was no inhibition, and influx was a hyperbolic function of [Na + ] o . The kinetics of Na + -inhibited Na + influx were analyzed at various extents of osmotic shrinkage. The curves for inhibited Na + fluxes were sigmoid, indicating more than one Na + inhibitory site associated with each transporter. Shrinkage significantly increased the Na + concentration at half-maximal velocity of Na + -inhibited Na + influx, the mechanism by which shrinkage activates NHE.
erythrocytes; cell volume regulation; amiloride; kinetics of sodium ion influx
Address for reprint requests and other correspondence: P. B. Dunham, BRL, Syracuse Univ., 130 College Pl., Syracuse, NY 132441220 (E-mail: pbdunham{at}syr.edu ). |
| doi_str_mv | 10.1152/ajpcell.00582.2003 |
| format | Article |
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Submitted 30 December 2003
; accepted in final form 18 March 2004
Na + /H + exchangers (NHE) are ubiquitous transporters participating in regulation of cell volume and pH. Cell shrinkage, acidification, and growth factors activate NHE by increasing its sensitivity to intracellular H + concentration. In this study, the kinetics were studied in dog red blood cells of Na + influx through NHE as a function of external Na + concentration ([Na + ] o ). In cells in isotonic media, [Na + ] o inhibited Na + influx >40 mM. Osmotic shrinkage activated NHE by reducing this inhibition. In cells in isotonic media + 120 mM sucrose, there was no inhibition, and influx was a hyperbolic function of [Na + ] o . The kinetics of Na + -inhibited Na + influx were analyzed at various extents of osmotic shrinkage. The curves for inhibited Na + fluxes were sigmoid, indicating more than one Na + inhibitory site associated with each transporter. Shrinkage significantly increased the Na + concentration at half-maximal velocity of Na + -inhibited Na + influx, the mechanism by which shrinkage activates NHE.
erythrocytes; cell volume regulation; amiloride; kinetics of sodium ion influx
Address for reprint requests and other correspondence: P. B. Dunham, BRL, Syracuse Univ., 130 College Pl., Syracuse, NY 132441220 (E-mail: pbdunham{at}syr.edu ).</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.00582.2003</identifier><identifier>PMID: 15070809</identifier><language>eng</language><publisher>United States</publisher><subject>Acids - pharmacology ; Amiloride - analogs & derivatives ; Animals ; Biological Transport - drug effects ; Biological Transport - physiology ; Diuretics - pharmacology ; Dogs ; Erythrocytes - drug effects ; Erythrocytes - metabolism ; Isotonic Solutions - pharmacology ; Models, Biological ; Osmolar Concentration ; Saline Solution, Hypertonic - pharmacology ; Sodium - pharmacokinetics ; Sodium-Hydrogen Exchangers - metabolism ; Sucrose - pharmacology ; Water-Electrolyte Balance - physiology</subject><ispartof>American Journal of Physiology: Cell Physiology, 2004-08, Vol.287 (2), p.C336-C344</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-e578306c97da1ced9cdba2b2d0977c73aaef57f01f969fc4f8c4891bc438e8d93</citedby><cites>FETCH-LOGICAL-c387t-e578306c97da1ced9cdba2b2d0977c73aaef57f01f969fc4f8c4891bc438e8d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15070809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dunham, Philip B</creatorcontrib><creatorcontrib>Kelley, Scott J</creatorcontrib><creatorcontrib>Logue, Paul J</creatorcontrib><title>Extracellular Na+ inhibits Na+/H+ exchange: cell shrinkage reduces the inhibition</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol Cell Physiol</addtitle><description>Department of Biology, Syracuse University, Syracuse, New York 13244-1220
Submitted 30 December 2003
; accepted in final form 18 March 2004
Na + /H + exchangers (NHE) are ubiquitous transporters participating in regulation of cell volume and pH. Cell shrinkage, acidification, and growth factors activate NHE by increasing its sensitivity to intracellular H + concentration. In this study, the kinetics were studied in dog red blood cells of Na + influx through NHE as a function of external Na + concentration ([Na + ] o ). In cells in isotonic media, [Na + ] o inhibited Na + influx >40 mM. Osmotic shrinkage activated NHE by reducing this inhibition. In cells in isotonic media + 120 mM sucrose, there was no inhibition, and influx was a hyperbolic function of [Na + ] o . The kinetics of Na + -inhibited Na + influx were analyzed at various extents of osmotic shrinkage. The curves for inhibited Na + fluxes were sigmoid, indicating more than one Na + inhibitory site associated with each transporter. Shrinkage significantly increased the Na + concentration at half-maximal velocity of Na + -inhibited Na + influx, the mechanism by which shrinkage activates NHE.
erythrocytes; cell volume regulation; amiloride; kinetics of sodium ion influx
Address for reprint requests and other correspondence: P. B. Dunham, BRL, Syracuse Univ., 130 College Pl., Syracuse, NY 132441220 (E-mail: pbdunham{at}syr.edu ).</description><subject>Acids - pharmacology</subject><subject>Amiloride - analogs & derivatives</subject><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Biological Transport - physiology</subject><subject>Diuretics - pharmacology</subject><subject>Dogs</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - metabolism</subject><subject>Isotonic Solutions - pharmacology</subject><subject>Models, Biological</subject><subject>Osmolar Concentration</subject><subject>Saline Solution, Hypertonic - pharmacology</subject><subject>Sodium - pharmacokinetics</subject><subject>Sodium-Hydrogen Exchangers - metabolism</subject><subject>Sucrose - pharmacology</subject><subject>Water-Electrolyte Balance - physiology</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKw0AUhgdRtF5ewIVk5UZS55LMxZ2UegFRBF0Pk8lJM5omcSbB9u1NbIsrV4fD-b6fw4_QOcFTQlJ6bT5aC1U1xTiVdEoxZntoMhxoTFLO9tEEM85iThJ2hI5D-MAYJ5SrQ3REUiywxGqCXuerzpsxpq-Mj57NVeTq0mWuC-Ny_XAVwcqWpl7ATTRiUSi9qz_NAiIPeW8hRF0JO8k19Sk6KEwV4Gw7T9D73fxt9hA_vdw_zm6fYsuk6GJIhWSYWyVyQyzkyuaZoRnNsRLCCmYMFKkoMCkUV4VNCmkTqUhmEyZB5oqdoMtNbuubrx5Cp5cujB-aGpo-aM654JKlA0g3oPVNCB4K3Xq3NH6tCdZjkXpbpP4tUo9FDtLFNr3PlpD_KdvmBkBtgNItym_nQbflOrimahZrfddX1Rusul0ylUJTPWOM6zYvBjf-39098-ewH3pClbQ</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Dunham, Philip B</creator><creator>Kelley, Scott J</creator><creator>Logue, Paul J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Extracellular Na+ inhibits Na+/H+ exchange: cell shrinkage reduces the inhibition</title><author>Dunham, Philip B ; Kelley, Scott J ; Logue, Paul J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-e578306c97da1ced9cdba2b2d0977c73aaef57f01f969fc4f8c4891bc438e8d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acids - pharmacology</topic><topic>Amiloride - analogs & derivatives</topic><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Biological Transport - physiology</topic><topic>Diuretics - pharmacology</topic><topic>Dogs</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - metabolism</topic><topic>Isotonic Solutions - pharmacology</topic><topic>Models, Biological</topic><topic>Osmolar Concentration</topic><topic>Saline Solution, Hypertonic - pharmacology</topic><topic>Sodium - pharmacokinetics</topic><topic>Sodium-Hydrogen Exchangers - metabolism</topic><topic>Sucrose - pharmacology</topic><topic>Water-Electrolyte Balance - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dunham, Philip B</creatorcontrib><creatorcontrib>Kelley, Scott J</creatorcontrib><creatorcontrib>Logue, Paul J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dunham, Philip B</au><au>Kelley, Scott J</au><au>Logue, Paul J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular Na+ inhibits Na+/H+ exchange: cell shrinkage reduces the inhibition</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol Cell Physiol</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>287</volume><issue>2</issue><spage>C336</spage><epage>C344</epage><pages>C336-C344</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>Department of Biology, Syracuse University, Syracuse, New York 13244-1220
Submitted 30 December 2003
; accepted in final form 18 March 2004
Na + /H + exchangers (NHE) are ubiquitous transporters participating in regulation of cell volume and pH. Cell shrinkage, acidification, and growth factors activate NHE by increasing its sensitivity to intracellular H + concentration. In this study, the kinetics were studied in dog red blood cells of Na + influx through NHE as a function of external Na + concentration ([Na + ] o ). In cells in isotonic media, [Na + ] o inhibited Na + influx >40 mM. Osmotic shrinkage activated NHE by reducing this inhibition. In cells in isotonic media + 120 mM sucrose, there was no inhibition, and influx was a hyperbolic function of [Na + ] o . The kinetics of Na + -inhibited Na + influx were analyzed at various extents of osmotic shrinkage. The curves for inhibited Na + fluxes were sigmoid, indicating more than one Na + inhibitory site associated with each transporter. Shrinkage significantly increased the Na + concentration at half-maximal velocity of Na + -inhibited Na + influx, the mechanism by which shrinkage activates NHE.
erythrocytes; cell volume regulation; amiloride; kinetics of sodium ion influx
Address for reprint requests and other correspondence: P. B. Dunham, BRL, Syracuse Univ., 130 College Pl., Syracuse, NY 132441220 (E-mail: pbdunham{at}syr.edu ).</abstract><cop>United States</cop><pmid>15070809</pmid><doi>10.1152/ajpcell.00582.2003</doi></addata></record> |
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| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 2004-08, Vol.287 (2), p.C336-C344 |
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| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Acids - pharmacology Amiloride - analogs & derivatives Animals Biological Transport - drug effects Biological Transport - physiology Diuretics - pharmacology Dogs Erythrocytes - drug effects Erythrocytes - metabolism Isotonic Solutions - pharmacology Models, Biological Osmolar Concentration Saline Solution, Hypertonic - pharmacology Sodium - pharmacokinetics Sodium-Hydrogen Exchangers - metabolism Sucrose - pharmacology Water-Electrolyte Balance - physiology |
| title | Extracellular Na+ inhibits Na+/H+ exchange: cell shrinkage reduces the inhibition |
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