Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta
George M. O'Brien Kidney and Urological Disease Center, Renal Division, Departments of Medicine, Cell Biology, and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110 To delineate the roles that oxygen and fibroblast growth factors (FGFs) play in the process of angio...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 2003-02, Vol.284 (2), p.C371-C377 |
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| container_title | American Journal of Physiology: Cell Physiology |
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| creator | Akimoto, Tetsu Hammerman, Marc R |
| description | George M. O'Brien Kidney and Urological Disease Center,
Renal Division, Departments of Medicine, Cell Biology, and Physiology,
Washington University School of Medicine, St. Louis, Missouri 63110
To delineate the roles that oxygen
and fibroblast growth factors (FGFs) play in the process of
angiogenesis from the embryonic aorta, we cultured mouse embryonic
aorta explants (thoracic level to lateral vessels supplying the
mesonephros and metanephros) in a three-dimensional type I collagen gel
matrix. During 8 days of culture under 5% O 2 , but not room
air, the addition of FGF2 to explants stimulated the formation of
Gs-IB 4- positive, CD31-positive, and Flk-1-positive
microvessels in a concentration-dependent manner. FGF2-stimulated
microvessel formation was inhibited by sequestration of FGF2 via
addition of soluble FGF receptor (FGFR) chimera protein or anti-FGF2
antibodies. FGFR1 and FGFR2 were present on explants. Levels of FGFR1,
but not FGFR2, were increased in embryonic aorta cultured under 5%
O 2 relative to room air. Our data suggest that low oxygen
upregulates FGFR1 expression in embryonic aorta in vitro and renders it
more responsive to FGF2.
angiogenesis; embryogenesis; endothelial cell; organ culture |
| doi_str_mv | 10.1152/ajpcell.00193.2002 |
| format | Article |
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Renal Division, Departments of Medicine, Cell Biology, and Physiology,
Washington University School of Medicine, St. Louis, Missouri 63110
To delineate the roles that oxygen
and fibroblast growth factors (FGFs) play in the process of
angiogenesis from the embryonic aorta, we cultured mouse embryonic
aorta explants (thoracic level to lateral vessels supplying the
mesonephros and metanephros) in a three-dimensional type I collagen gel
matrix. During 8 days of culture under 5% O 2 , but not room
air, the addition of FGF2 to explants stimulated the formation of
Gs-IB 4- positive, CD31-positive, and Flk-1-positive
microvessels in a concentration-dependent manner. FGF2-stimulated
microvessel formation was inhibited by sequestration of FGF2 via
addition of soluble FGF receptor (FGFR) chimera protein or anti-FGF2
antibodies. FGFR1 and FGFR2 were present on explants. Levels of FGFR1,
but not FGFR2, were increased in embryonic aorta cultured under 5%
O 2 relative to room air. Our data suggest that low oxygen
upregulates FGFR1 expression in embryonic aorta in vitro and renders it
more responsive to FGF2.
angiogenesis; embryogenesis; endothelial cell; organ culture</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.00193.2002</identifier><identifier>PMID: 12388106</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Aorta - drug effects ; Aorta - embryology ; Aorta - metabolism ; Capillaries - drug effects ; Capillaries - embryology ; Capillaries - metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelial Growth Factors - metabolism ; Endothelial Growth Factors - pharmacology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Fetus ; Fibroblast Growth Factor 2 - antagonists & inhibitors ; Fibroblast Growth Factor 2 - metabolism ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Developmental - physiology ; Intercellular Signaling Peptides and Proteins - metabolism ; Intercellular Signaling Peptides and Proteins - pharmacology ; Lymphokines - metabolism ; Lymphokines - pharmacology ; Mice ; Mice, Inbred C57BL ; Microcirculation - drug effects ; Microcirculation - embryology ; Microcirculation - metabolism ; Neovascularization, Physiologic - drug effects ; Neovascularization, Physiologic - physiology ; Oxygen - pharmacology ; Receptor Protein-Tyrosine Kinases - drug effects ; Receptor Protein-Tyrosine Kinases - metabolism ; Receptor Protein-Tyrosine Kinases - pharmacology ; Receptor, Fibroblast Growth Factor, Type 1 ; Receptor, Fibroblast Growth Factor, Type 2 ; Receptors, Fibroblast Growth Factor - drug effects ; Receptors, Fibroblast Growth Factor - metabolism ; Recombinant Fusion Proteins ; Up-Regulation - drug effects ; Up-Regulation - physiology ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>American Journal of Physiology: Cell Physiology, 2003-02, Vol.284 (2), p.C371-C377</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-e55c954d24d9a444cfc5dcd3ea0f2bcce0e9d7a3faba827ad0bcf79a9960025b3</citedby><cites>FETCH-LOGICAL-c453t-e55c954d24d9a444cfc5dcd3ea0f2bcce0e9d7a3faba827ad0bcf79a9960025b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3026,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12388106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akimoto, Tetsu</creatorcontrib><creatorcontrib>Hammerman, Marc R</creatorcontrib><title>Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol Cell Physiol</addtitle><description>George M. O'Brien Kidney and Urological Disease Center,
Renal Division, Departments of Medicine, Cell Biology, and Physiology,
Washington University School of Medicine, St. Louis, Missouri 63110
To delineate the roles that oxygen
and fibroblast growth factors (FGFs) play in the process of
angiogenesis from the embryonic aorta, we cultured mouse embryonic
aorta explants (thoracic level to lateral vessels supplying the
mesonephros and metanephros) in a three-dimensional type I collagen gel
matrix. During 8 days of culture under 5% O 2 , but not room
air, the addition of FGF2 to explants stimulated the formation of
Gs-IB 4- positive, CD31-positive, and Flk-1-positive
microvessels in a concentration-dependent manner. FGF2-stimulated
microvessel formation was inhibited by sequestration of FGF2 via
addition of soluble FGF receptor (FGFR) chimera protein or anti-FGF2
antibodies. FGFR1 and FGFR2 were present on explants. Levels of FGFR1,
but not FGFR2, were increased in embryonic aorta cultured under 5%
O 2 relative to room air. Our data suggest that low oxygen
upregulates FGFR1 expression in embryonic aorta in vitro and renders it
more responsive to FGF2.
angiogenesis; embryogenesis; endothelial cell; organ culture</description><subject>Animals</subject><subject>Aorta - drug effects</subject><subject>Aorta - embryology</subject><subject>Aorta - metabolism</subject><subject>Capillaries - drug effects</subject><subject>Capillaries - embryology</subject><subject>Capillaries - metabolism</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelial Growth Factors - metabolism</subject><subject>Endothelial Growth Factors - pharmacology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fetus</subject><subject>Fibroblast Growth Factor 2 - antagonists & inhibitors</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - pharmacology</subject><subject>Lymphokines - metabolism</subject><subject>Lymphokines - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microcirculation - drug effects</subject><subject>Microcirculation - embryology</subject><subject>Microcirculation - metabolism</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Oxygen - pharmacology</subject><subject>Receptor Protein-Tyrosine Kinases - drug effects</subject><subject>Receptor Protein-Tyrosine Kinases - metabolism</subject><subject>Receptor Protein-Tyrosine Kinases - pharmacology</subject><subject>Receptor, Fibroblast Growth Factor, Type 1</subject><subject>Receptor, Fibroblast Growth Factor, Type 2</subject><subject>Receptors, Fibroblast Growth Factor - drug effects</subject><subject>Receptors, Fibroblast Growth Factor - metabolism</subject><subject>Recombinant Fusion Proteins</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - physiology</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtOwzAUhi0EgnJ5AQbkiS3FtyT1iCoKSEgsZbYc57g1Supgu0DfHpe2YmI6w3_Rfz6ErikZU1qyO_0-GOi6MSFU8jEjhB2hURZYQcuKH6MR4RUvKir4GTqP8Z0QIlglT9EZZXwyoaQaofnMNcE3nY4JL4L_SktstUk-YIaH4HufIOLemeA_IUbosPWh18n5FbZZxr1fR8DQN2HjV85g7UPSl-jE6i7C1f5eoLfZw3z6VLy8Pj5P718KI0qeCihLI0vRMtFKLYQw1pStaTloYlljDBCQba251Y2esFq3pDG2llrKKv9aNvwC3e5689KPNcSkehe3SPQK8i5VM1lJwUg2sp0x_xFjAKuG4HodNooStWWp9izVL0u1ZZlDN_v2ddND-xfZw8sGuTMs3WL55QKoYbmJznd-sVGzddfN4TsdmtlEKKamvKZqaG3OFv9nD2P-MvwHwUSZFw</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Akimoto, Tetsu</creator><creator>Hammerman, Marc R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta</title><author>Akimoto, Tetsu ; Hammerman, Marc R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-e55c954d24d9a444cfc5dcd3ea0f2bcce0e9d7a3faba827ad0bcf79a9960025b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Aorta - drug effects</topic><topic>Aorta - embryology</topic><topic>Aorta - metabolism</topic><topic>Capillaries - drug effects</topic><topic>Capillaries - embryology</topic><topic>Capillaries - metabolism</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelial Growth Factors - metabolism</topic><topic>Endothelial Growth Factors - pharmacology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fetus</topic><topic>Fibroblast Growth Factor 2 - antagonists & inhibitors</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Intercellular Signaling Peptides and Proteins - pharmacology</topic><topic>Lymphokines - metabolism</topic><topic>Lymphokines - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microcirculation - drug effects</topic><topic>Microcirculation - embryology</topic><topic>Microcirculation - metabolism</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Oxygen - pharmacology</topic><topic>Receptor Protein-Tyrosine Kinases - drug effects</topic><topic>Receptor Protein-Tyrosine Kinases - metabolism</topic><topic>Receptor Protein-Tyrosine Kinases - pharmacology</topic><topic>Receptor, Fibroblast Growth Factor, Type 1</topic><topic>Receptor, Fibroblast Growth Factor, Type 2</topic><topic>Receptors, Fibroblast Growth Factor - drug effects</topic><topic>Receptors, Fibroblast Growth Factor - metabolism</topic><topic>Recombinant Fusion Proteins</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - physiology</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akimoto, Tetsu</creatorcontrib><creatorcontrib>Hammerman, Marc R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akimoto, Tetsu</au><au>Hammerman, Marc R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol Cell Physiol</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>284</volume><issue>2</issue><spage>C371</spage><epage>C377</epage><pages>C371-C377</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>George M. O'Brien Kidney and Urological Disease Center,
Renal Division, Departments of Medicine, Cell Biology, and Physiology,
Washington University School of Medicine, St. Louis, Missouri 63110
To delineate the roles that oxygen
and fibroblast growth factors (FGFs) play in the process of
angiogenesis from the embryonic aorta, we cultured mouse embryonic
aorta explants (thoracic level to lateral vessels supplying the
mesonephros and metanephros) in a three-dimensional type I collagen gel
matrix. During 8 days of culture under 5% O 2 , but not room
air, the addition of FGF2 to explants stimulated the formation of
Gs-IB 4- positive, CD31-positive, and Flk-1-positive
microvessels in a concentration-dependent manner. FGF2-stimulated
microvessel formation was inhibited by sequestration of FGF2 via
addition of soluble FGF receptor (FGFR) chimera protein or anti-FGF2
antibodies. FGFR1 and FGFR2 were present on explants. Levels of FGFR1,
but not FGFR2, were increased in embryonic aorta cultured under 5%
O 2 relative to room air. Our data suggest that low oxygen
upregulates FGFR1 expression in embryonic aorta in vitro and renders it
more responsive to FGF2.
angiogenesis; embryogenesis; endothelial cell; organ culture</abstract><cop>United States</cop><pmid>12388106</pmid><doi>10.1152/ajpcell.00193.2002</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 2003-02, Vol.284 (2), p.C371-C377 |
| issn | 0363-6143 1522-1563 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpcell_284_2_C371 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Aorta - drug effects Aorta - embryology Aorta - metabolism Capillaries - drug effects Capillaries - embryology Capillaries - metabolism Cells, Cultured Dose-Response Relationship, Drug Endothelial Growth Factors - metabolism Endothelial Growth Factors - pharmacology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fetus Fibroblast Growth Factor 2 - antagonists & inhibitors Fibroblast Growth Factor 2 - metabolism Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Developmental - physiology Intercellular Signaling Peptides and Proteins - metabolism Intercellular Signaling Peptides and Proteins - pharmacology Lymphokines - metabolism Lymphokines - pharmacology Mice Mice, Inbred C57BL Microcirculation - drug effects Microcirculation - embryology Microcirculation - metabolism Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - physiology Oxygen - pharmacology Receptor Protein-Tyrosine Kinases - drug effects Receptor Protein-Tyrosine Kinases - metabolism Receptor Protein-Tyrosine Kinases - pharmacology Receptor, Fibroblast Growth Factor, Type 1 Receptor, Fibroblast Growth Factor, Type 2 Receptors, Fibroblast Growth Factor - drug effects Receptors, Fibroblast Growth Factor - metabolism Recombinant Fusion Proteins Up-Regulation - drug effects Up-Regulation - physiology Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta |
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