Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore
Helsinki Biophysics and Biomembranes Group, Department of Medical Chemistry, Institute of Biomedicine, FIN-00014 University of Helsinki, Helsinki, Finland Triggering of the permeability transition pore (PTP) in isolated mitochondria causes release of matrix Ca 2+ , ions, and metabolites, and it has...
Gespeichert in:
| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 1999-06, Vol.276 (6), p.C1297-C1302 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | C1302 |
|---|---|
| container_issue | 6 |
| container_start_page | C1297 |
| container_title | American Journal of Physiology: Cell Physiology |
| container_volume | 276 |
| creator | Eriksson, Ove Pollesello, Piero Geimonen, Erika |
| description | Helsinki Biophysics and Biomembranes Group, Department of Medical
Chemistry, Institute of Biomedicine, FIN-00014 University of
Helsinki, Helsinki, Finland
Triggering of
the permeability transition pore (PTP) in isolated mitochondria causes
release of matrix Ca 2+ , ions, and
metabolites, and it has been proposed that the PTP mediates
mitochondrial Ca 2+ release in
intact cells. To study the role of the PTP in mitochondrial energy
metabolism, the mitochondrial content of
Ca 2+ ,
Mg 2+ , ATP, and ADP was determined
in hormonally stimulated rat livers perfused with cyclosporin A (CsA).
Stimulation of livers perfused in the absence of CsA with glucagon and
phenylephrine induced an extensive uptake of
Ca 2+ ,
Mg 2+ , and ATP plus ADP by the
mitochondria, followed by a release on omission of hormones. In the
presence of CsA, the PTP was fully inhibited, but neither the
hormone-induced uptake of Ca 2+ ,
ATP, or ADP by mitochondria nor their release after washout of hormones
was significantly changed. We conclude that the regulation of sustained
changes in mitochondrial Ca 2+
content induced by hormonal stimulation is independent of the PTP.
cyclosporin A; permeability transition; mitochondria; calcium; intact rat liver |
| doi_str_mv | 10.1152/ajpcell.1999.276.6.C1297 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_highw</sourceid><recordid>TN_cdi_highwire_physiology_ajpcell_276_6_C1297</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69815153</sourcerecordid><originalsourceid>FETCH-LOGICAL-h229t-823d607b977b3cdab191744ec83cb21d473d7f5b14899f0e8fa94570f4e5d03a3</originalsourceid><addsrcrecordid>eNp1kU1v1DAQhi0EotvCX0A-cUEJ_kjsmBtaUahUCQmVs-XEk42LEwfbAfbf4263EhcuHnnmeebwDkKYkprSlr039-sA3tdUKVUzKWpR7ylT8hnalTGraCv4c7QjXPBK0IZfoMuU7gkhDRPqJbqgZcJaxXboxzc4bN5kFxYcRpxDNh7PLodhCouNrvz2hr3DbsErxHFLYLF3vyBil0rTwgrlWfJJnuABmsH0zrt8xDmaJbnT7jVEeIVejMYneH2uV-j79ae7_Zfq9uvnm_3H22piTOWqY9wKInslZc8Ha3qqqGwaGDo-9IzaRnIrx7anTafUSKAbjWpaScYGWku44Vfo7ePeNYafG6SsZ5ce8jILhC1poTra0pYX8M0Z3PoZrF6jm0086qd4ClA_ApM7TL9dBL1Ox-SCD4ejPh9Bl_y10Kf8i_Dh_8L15v0d_MlP5j-iXu3I_wLV-5Dm</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69815153</pqid></control><display><type>article</type><title>Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore</title><source>MEDLINE</source><source>American Physiological Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Eriksson, Ove ; Pollesello, Piero ; Geimonen, Erika</creator><creatorcontrib>Eriksson, Ove ; Pollesello, Piero ; Geimonen, Erika</creatorcontrib><description>Helsinki Biophysics and Biomembranes Group, Department of Medical
Chemistry, Institute of Biomedicine, FIN-00014 University of
Helsinki, Helsinki, Finland
Triggering of
the permeability transition pore (PTP) in isolated mitochondria causes
release of matrix Ca 2+ , ions, and
metabolites, and it has been proposed that the PTP mediates
mitochondrial Ca 2+ release in
intact cells. To study the role of the PTP in mitochondrial energy
metabolism, the mitochondrial content of
Ca 2+ ,
Mg 2+ , ATP, and ADP was determined
in hormonally stimulated rat livers perfused with cyclosporin A (CsA).
Stimulation of livers perfused in the absence of CsA with glucagon and
phenylephrine induced an extensive uptake of
Ca 2+ ,
Mg 2+ , and ATP plus ADP by the
mitochondria, followed by a release on omission of hormones. In the
presence of CsA, the PTP was fully inhibited, but neither the
hormone-induced uptake of Ca 2+ ,
ATP, or ADP by mitochondria nor their release after washout of hormones
was significantly changed. We conclude that the regulation of sustained
changes in mitochondrial Ca 2+
content induced by hormonal stimulation is independent of the PTP.
cyclosporin A; permeability transition; mitochondria; calcium; intact rat liver</description><identifier>ISSN: 0363-6143</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.1999.276.6.C1297</identifier><identifier>PMID: 10362592</identifier><language>eng</language><publisher>United States</publisher><subject>Adenosine Diphosphate - metabolism ; Adenosine Triphosphate - metabolism ; Animals ; Calcium - metabolism ; Cyclosporine - pharmacology ; Glucagon - pharmacology ; Immunosuppressive Agents - pharmacology ; Magnesium - metabolism ; Male ; Mitochondria, Liver - metabolism ; Perfusion ; Permeability ; Phenylephrine - pharmacology ; Rats ; Rats, Wistar</subject><ispartof>American Journal of Physiology: Cell Physiology, 1999-06, Vol.276 (6), p.C1297-C1302</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10362592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eriksson, Ove</creatorcontrib><creatorcontrib>Pollesello, Piero</creatorcontrib><creatorcontrib>Geimonen, Erika</creatorcontrib><title>Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol</addtitle><description>Helsinki Biophysics and Biomembranes Group, Department of Medical
Chemistry, Institute of Biomedicine, FIN-00014 University of
Helsinki, Helsinki, Finland
Triggering of
the permeability transition pore (PTP) in isolated mitochondria causes
release of matrix Ca 2+ , ions, and
metabolites, and it has been proposed that the PTP mediates
mitochondrial Ca 2+ release in
intact cells. To study the role of the PTP in mitochondrial energy
metabolism, the mitochondrial content of
Ca 2+ ,
Mg 2+ , ATP, and ADP was determined
in hormonally stimulated rat livers perfused with cyclosporin A (CsA).
Stimulation of livers perfused in the absence of CsA with glucagon and
phenylephrine induced an extensive uptake of
Ca 2+ ,
Mg 2+ , and ATP plus ADP by the
mitochondria, followed by a release on omission of hormones. In the
presence of CsA, the PTP was fully inhibited, but neither the
hormone-induced uptake of Ca 2+ ,
ATP, or ADP by mitochondria nor their release after washout of hormones
was significantly changed. We conclude that the regulation of sustained
changes in mitochondrial Ca 2+
content induced by hormonal stimulation is independent of the PTP.
cyclosporin A; permeability transition; mitochondria; calcium; intact rat liver</description><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cyclosporine - pharmacology</subject><subject>Glucagon - pharmacology</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Magnesium - metabolism</subject><subject>Male</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Perfusion</subject><subject>Permeability</subject><subject>Phenylephrine - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0363-6143</issn><issn>0002-9513</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v1DAQhi0EotvCX0A-cUEJ_kjsmBtaUahUCQmVs-XEk42LEwfbAfbf4263EhcuHnnmeebwDkKYkprSlr039-sA3tdUKVUzKWpR7ylT8hnalTGraCv4c7QjXPBK0IZfoMuU7gkhDRPqJbqgZcJaxXboxzc4bN5kFxYcRpxDNh7PLodhCouNrvz2hr3DbsErxHFLYLF3vyBil0rTwgrlWfJJnuABmsH0zrt8xDmaJbnT7jVEeIVejMYneH2uV-j79ae7_Zfq9uvnm_3H22piTOWqY9wKInslZc8Ha3qqqGwaGDo-9IzaRnIrx7anTafUSKAbjWpaScYGWku44Vfo7ePeNYafG6SsZ5ce8jILhC1poTra0pYX8M0Z3PoZrF6jm0086qd4ClA_ApM7TL9dBL1Ox-SCD4ejPh9Bl_y10Kf8i_Dh_8L15v0d_MlP5j-iXu3I_wLV-5Dm</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Eriksson, Ove</creator><creator>Pollesello, Piero</creator><creator>Geimonen, Erika</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore</title><author>Eriksson, Ove ; Pollesello, Piero ; Geimonen, Erika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h229t-823d607b977b3cdab191744ec83cb21d473d7f5b14899f0e8fa94570f4e5d03a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Cyclosporine - pharmacology</topic><topic>Glucagon - pharmacology</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Magnesium - metabolism</topic><topic>Male</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Perfusion</topic><topic>Permeability</topic><topic>Phenylephrine - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eriksson, Ove</creatorcontrib><creatorcontrib>Pollesello, Piero</creatorcontrib><creatorcontrib>Geimonen, Erika</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eriksson, Ove</au><au>Pollesello, Piero</au><au>Geimonen, Erika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>276</volume><issue>6</issue><spage>C1297</spage><epage>C1302</epage><pages>C1297-C1302</pages><issn>0363-6143</issn><issn>0002-9513</issn><eissn>1522-1563</eissn><abstract>Helsinki Biophysics and Biomembranes Group, Department of Medical
Chemistry, Institute of Biomedicine, FIN-00014 University of
Helsinki, Helsinki, Finland
Triggering of
the permeability transition pore (PTP) in isolated mitochondria causes
release of matrix Ca 2+ , ions, and
metabolites, and it has been proposed that the PTP mediates
mitochondrial Ca 2+ release in
intact cells. To study the role of the PTP in mitochondrial energy
metabolism, the mitochondrial content of
Ca 2+ ,
Mg 2+ , ATP, and ADP was determined
in hormonally stimulated rat livers perfused with cyclosporin A (CsA).
Stimulation of livers perfused in the absence of CsA with glucagon and
phenylephrine induced an extensive uptake of
Ca 2+ ,
Mg 2+ , and ATP plus ADP by the
mitochondria, followed by a release on omission of hormones. In the
presence of CsA, the PTP was fully inhibited, but neither the
hormone-induced uptake of Ca 2+ ,
ATP, or ADP by mitochondria nor their release after washout of hormones
was significantly changed. We conclude that the regulation of sustained
changes in mitochondrial Ca 2+
content induced by hormonal stimulation is independent of the PTP.
cyclosporin A; permeability transition; mitochondria; calcium; intact rat liver</abstract><cop>United States</cop><pmid>10362592</pmid><doi>10.1152/ajpcell.1999.276.6.C1297</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 1999-06, Vol.276 (6), p.C1297-C1302 |
| issn | 0363-6143 0002-9513 1522-1563 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpcell_276_6_C1297 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Animals Calcium - metabolism Cyclosporine - pharmacology Glucagon - pharmacology Immunosuppressive Agents - pharmacology Magnesium - metabolism Male Mitochondria, Liver - metabolism Perfusion Permeability Phenylephrine - pharmacology Rats Rats, Wistar |
| title | Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T21%3A17%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_highw&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulation%20of%20total%20mitochondrial%20Ca2+%20in%20perfused%20liver%20is%20independent%20of%20the%20permeability%20transition%20pore&rft.jtitle=American%20Journal%20of%20Physiology:%20Cell%20Physiology&rft.au=Eriksson,%20Ove&rft.date=1999-06-01&rft.volume=276&rft.issue=6&rft.spage=C1297&rft.epage=C1302&rft.pages=C1297-C1302&rft.issn=0363-6143&rft.eissn=1522-1563&rft_id=info:doi/10.1152/ajpcell.1999.276.6.C1297&rft_dat=%3Cproquest_highw%3E69815153%3C/proquest_highw%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69815153&rft_id=info:pmid/10362592&rfr_iscdi=true |