Effects of extracellular pH, PCO2, and HCO3- on intracellular pH in isolated rat taste buds
V. Lyall, G. M. Feldman, G. L. Heck and J. A. DeSimone Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0551, USA. We studied the effects of changing external pH (pHo), external bicarbonate concentration ([HCO3-]o), and PCO2 on taste receptor ce...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 1997-09, Vol.273 (3), p.C1008-C1019 |
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| creator | Lyall, V Feldman, G. M Heck, G. L DeSimone, J. A |
| description | V. Lyall, G. M. Feldman, G. L. Heck and J. A. DeSimone
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0551, USA.
We studied the effects of changing external pH (pHo), external bicarbonate
concentration ([HCO3-]o), and PCO2 on taste receptor cell (TRC)
intracellular pH (pHi) in taste bud fragments (TBFs) isolated from rat
circumvallate and fungiform papillae with the pH-sensitive fluoroprobe
2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) using
microfluorometric and imaging techniques. In
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered solutions, TRC
pHi responded rapidly and monotonically to changes in pHo between 6.5 and
8.0. The relationship between pHi and pHo was steep, with slopes varying
between 0.8 and 1.2. Similarly, varying pHo by changing PCO2 at constant
[HCO3-]o or changing [HCO3-]o at constant PCO2 led to rapid, monotonic
changes in pHi. The relationship between pHi and pHo was once again steep,
with slopes varying between 0.8 and 1.2. However, simultaneous changes in
PCO2 and [HCO3-]o at constant pHo did not cause any significant changes in
steady-state pHi. In imaging studies, single, isolated TRCs responded to
changes in pHo, with parallel changes in pHi in the soma and apical
process. In addition, changes in pHo induced parallel changes in pHi
throughout TBFs. These data suggest that the steady-state TRC pHi is a
function of pHo. Changes in TRC pHi may be involved in acid sensing, and
salivary [HCO3-] may play a role in the maintainance of steady-state TRC
pHi and in the neutralization of acid-induced changes in pHi. |
| doi_str_mv | 10.1152/ajpcell.1997.273.3.c1008 |
| format | Article |
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Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0551, USA.
We studied the effects of changing external pH (pHo), external bicarbonate
concentration ([HCO3-]o), and PCO2 on taste receptor cell (TRC)
intracellular pH (pHi) in taste bud fragments (TBFs) isolated from rat
circumvallate and fungiform papillae with the pH-sensitive fluoroprobe
2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) using
microfluorometric and imaging techniques. In
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered solutions, TRC
pHi responded rapidly and monotonically to changes in pHo between 6.5 and
8.0. The relationship between pHi and pHo was steep, with slopes varying
between 0.8 and 1.2. Similarly, varying pHo by changing PCO2 at constant
[HCO3-]o or changing [HCO3-]o at constant PCO2 led to rapid, monotonic
changes in pHi. The relationship between pHi and pHo was once again steep,
with slopes varying between 0.8 and 1.2. However, simultaneous changes in
PCO2 and [HCO3-]o at constant pHo did not cause any significant changes in
steady-state pHi. In imaging studies, single, isolated TRCs responded to
changes in pHo, with parallel changes in pHi in the soma and apical
process. In addition, changes in pHo induced parallel changes in pHi
throughout TBFs. These data suggest that the steady-state TRC pHi is a
function of pHo. Changes in TRC pHi may be involved in acid sensing, and
salivary [HCO3-] may play a role in the maintainance of steady-state TRC
pHi and in the neutralization of acid-induced changes in pHi.</description><identifier>ISSN: 0363-6143</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.1997.273.3.c1008</identifier><identifier>PMID: 9316422</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Bicarbonates - pharmacology ; Carbon Dioxide - pharmacology ; Cell Survival ; Extracellular Space ; Female ; Fluoresceins ; Fluorescent Dyes ; Hydrogen-Ion Concentration ; In Vitro Techniques ; Kinetics ; Microscopy, Fluorescence - methods ; Partial Pressure ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Fluorescence - methods ; Taste Buds - cytology ; Taste Buds - drug effects ; Taste Buds - physiology</subject><ispartof>American Journal of Physiology: Cell Physiology, 1997-09, Vol.273 (3), p.C1008-C1019</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-668c61137c42b425077f866c3d2825fd25f83d2da2c73e87d27a376e927779723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9316422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lyall, V</creatorcontrib><creatorcontrib>Feldman, G. M</creatorcontrib><creatorcontrib>Heck, G. L</creatorcontrib><creatorcontrib>DeSimone, J. A</creatorcontrib><title>Effects of extracellular pH, PCO2, and HCO3- on intracellular pH in isolated rat taste buds</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol</addtitle><description>V. Lyall, G. M. Feldman, G. L. Heck and J. A. DeSimone
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0551, USA.
We studied the effects of changing external pH (pHo), external bicarbonate
concentration ([HCO3-]o), and PCO2 on taste receptor cell (TRC)
intracellular pH (pHi) in taste bud fragments (TBFs) isolated from rat
circumvallate and fungiform papillae with the pH-sensitive fluoroprobe
2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) using
microfluorometric and imaging techniques. In
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered solutions, TRC
pHi responded rapidly and monotonically to changes in pHo between 6.5 and
8.0. The relationship between pHi and pHo was steep, with slopes varying
between 0.8 and 1.2. Similarly, varying pHo by changing PCO2 at constant
[HCO3-]o or changing [HCO3-]o at constant PCO2 led to rapid, monotonic
changes in pHi. The relationship between pHi and pHo was once again steep,
with slopes varying between 0.8 and 1.2. However, simultaneous changes in
PCO2 and [HCO3-]o at constant pHo did not cause any significant changes in
steady-state pHi. In imaging studies, single, isolated TRCs responded to
changes in pHo, with parallel changes in pHi in the soma and apical
process. In addition, changes in pHo induced parallel changes in pHi
throughout TBFs. These data suggest that the steady-state TRC pHi is a
function of pHo. Changes in TRC pHi may be involved in acid sensing, and
salivary [HCO3-] may play a role in the maintainance of steady-state TRC
pHi and in the neutralization of acid-induced changes in pHi.</description><subject>Animals</subject><subject>Bicarbonates - pharmacology</subject><subject>Carbon Dioxide - pharmacology</subject><subject>Cell Survival</subject><subject>Extracellular Space</subject><subject>Female</subject><subject>Fluoresceins</subject><subject>Fluorescent Dyes</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Partial Pressure</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Taste Buds - cytology</subject><subject>Taste Buds - drug effects</subject><subject>Taste Buds - physiology</subject><issn>0363-6143</issn><issn>0002-9513</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkF9LwzAUxYMoc04_gpAnn9aaP23SPkqZThjMB33yIWRJunV0TU1adN_elJWhD-GGnHPPzf0BADGKMU7Jo9y3ytR1jPOcx4TTmMYKI5RdgGmQSYRTRi_BFFFGI4YTeg1uvN8jhBLC8gmY5BSzhJAp-FyUpVGdh7aE5qdzcojta-lgu5zDt2JN5lA2Gi6LNY2gbWDV_DeFB1h5W8vOaOhkBzvpOwM3vfa34KqUtTd3Y52Bj-fFe7GMVuuX1-JpFSnCki5iLFMMY8pVQjYJSRHnZcaYoppkJC11OFm4a0kUpybjmnBJOTM54ZznnNAZeDjlts5-9cZ34lD54YuyMbb3godt84QNxuxkVM5670wpWlcdpDsKjMTAVYxcxcBVBK6CimLgGlrvxxn95mD0uXEEGfT4pO-q7e67cka0u6OvbG23x3Pq38BfUGWD4w</recordid><startdate>199709</startdate><enddate>199709</enddate><creator>Lyall, V</creator><creator>Feldman, G. M</creator><creator>Heck, G. L</creator><creator>DeSimone, J. A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199709</creationdate><title>Effects of extracellular pH, PCO2, and HCO3- on intracellular pH in isolated rat taste buds</title><author>Lyall, V ; Feldman, G. M ; Heck, G. L ; DeSimone, J. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-668c61137c42b425077f866c3d2825fd25f83d2da2c73e87d27a376e927779723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Bicarbonates - pharmacology</topic><topic>Carbon Dioxide - pharmacology</topic><topic>Cell Survival</topic><topic>Extracellular Space</topic><topic>Female</topic><topic>Fluoresceins</topic><topic>Fluorescent Dyes</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Partial Pressure</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Taste Buds - cytology</topic><topic>Taste Buds - drug effects</topic><topic>Taste Buds - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lyall, V</creatorcontrib><creatorcontrib>Feldman, G. M</creatorcontrib><creatorcontrib>Heck, G. L</creatorcontrib><creatorcontrib>DeSimone, J. A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lyall, V</au><au>Feldman, G. M</au><au>Heck, G. L</au><au>DeSimone, J. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of extracellular pH, PCO2, and HCO3- on intracellular pH in isolated rat taste buds</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1997-09</date><risdate>1997</risdate><volume>273</volume><issue>3</issue><spage>C1008</spage><epage>C1019</epage><pages>C1008-C1019</pages><issn>0363-6143</issn><issn>0002-9513</issn><eissn>1522-1563</eissn><abstract>V. Lyall, G. M. Feldman, G. L. Heck and J. A. DeSimone
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0551, USA.
We studied the effects of changing external pH (pHo), external bicarbonate
concentration ([HCO3-]o), and PCO2 on taste receptor cell (TRC)
intracellular pH (pHi) in taste bud fragments (TBFs) isolated from rat
circumvallate and fungiform papillae with the pH-sensitive fluoroprobe
2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) using
microfluorometric and imaging techniques. In
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered solutions, TRC
pHi responded rapidly and monotonically to changes in pHo between 6.5 and
8.0. The relationship between pHi and pHo was steep, with slopes varying
between 0.8 and 1.2. Similarly, varying pHo by changing PCO2 at constant
[HCO3-]o or changing [HCO3-]o at constant PCO2 led to rapid, monotonic
changes in pHi. The relationship between pHi and pHo was once again steep,
with slopes varying between 0.8 and 1.2. However, simultaneous changes in
PCO2 and [HCO3-]o at constant pHo did not cause any significant changes in
steady-state pHi. In imaging studies, single, isolated TRCs responded to
changes in pHo, with parallel changes in pHi in the soma and apical
process. In addition, changes in pHo induced parallel changes in pHi
throughout TBFs. These data suggest that the steady-state TRC pHi is a
function of pHo. Changes in TRC pHi may be involved in acid sensing, and
salivary [HCO3-] may play a role in the maintainance of steady-state TRC
pHi and in the neutralization of acid-induced changes in pHi.</abstract><cop>United States</cop><pmid>9316422</pmid><doi>10.1152/ajpcell.1997.273.3.c1008</doi></addata></record> |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bicarbonates - pharmacology Carbon Dioxide - pharmacology Cell Survival Extracellular Space Female Fluoresceins Fluorescent Dyes Hydrogen-Ion Concentration In Vitro Techniques Kinetics Microscopy, Fluorescence - methods Partial Pressure Rats Rats, Sprague-Dawley Spectrometry, Fluorescence - methods Taste Buds - cytology Taste Buds - drug effects Taste Buds - physiology |
| title | Effects of extracellular pH, PCO2, and HCO3- on intracellular pH in isolated rat taste buds |
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