Altered E-C coupling in triads isolated from malignant hyperthermia-susceptible porcine muscle
R. el-Hayek, M. Yano, B. Antoniu, J. R. Mickelson, C. F. Louis and N. Ikemoto Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA. Triad vesicles were isolated from normal (N) and homozygous malignant hyperthermia-susceptible (MHS) porcine skeletal muscle, and two types of...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 1995-06, Vol.268 (6), p.C1381-C1386 |
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| container_title | American Journal of Physiology: Cell Physiology |
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| creator | el-Hayek, R Yano, M Antoniu, B Mickelson, J. R Louis, C. F Ikemoto, N |
| description | R. el-Hayek, M. Yano, B. Antoniu, J. R. Mickelson, C. F. Louis and N. Ikemoto
Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA.
Triad vesicles were isolated from normal (N) and homozygous malignant
hyperthermia-susceptible (MHS) porcine skeletal muscle, and two types of
sarcoplasmic reticulum Ca2+ release were investigated: 1)
polylysine-induced Ca2+ release (direct stimulation of the junctional foot
protein), and 2) depolarization-induced Ca2+ release (stimulation of the
junctional foot protein via the dihydropyridine receptor). At submaximal
concentrations of polylysine, the rates of induced Ca2+ release from the
MHS triads were greater than from normal triads. The T tubules of polarized
triads were depolarized by the K(+)-to-Na+ ionic replacement protocol.
Higher grades of T-tubule depolarization resulted in higher rates of Ca2+
release from both MHS and normal triads but, when compared at a given grade
of T-tubule depolarization, the release rate was always greater from the
MHS than from normal triads. Thus the activity of the SR Ca2+ release
channel is always higher in MHS than in normal muscle at a given submaximal
dose of release trigger. This difference is observed when the channel is
stimulated directly by polylysine or indirectly via a
depolarization-induced activation of the T-tubule dihydropyridine receptor. |
| doi_str_mv | 10.1152/ajpcell.1995.268.6.c1381 |
| format | Article |
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Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA.
Triad vesicles were isolated from normal (N) and homozygous malignant
hyperthermia-susceptible (MHS) porcine skeletal muscle, and two types of
sarcoplasmic reticulum Ca2+ release were investigated: 1)
polylysine-induced Ca2+ release (direct stimulation of the junctional foot
protein), and 2) depolarization-induced Ca2+ release (stimulation of the
junctional foot protein via the dihydropyridine receptor). At submaximal
concentrations of polylysine, the rates of induced Ca2+ release from the
MHS triads were greater than from normal triads. The T tubules of polarized
triads were depolarized by the K(+)-to-Na+ ionic replacement protocol.
Higher grades of T-tubule depolarization resulted in higher rates of Ca2+
release from both MHS and normal triads but, when compared at a given grade
of T-tubule depolarization, the release rate was always greater from the
MHS than from normal triads. Thus the activity of the SR Ca2+ release
channel is always higher in MHS than in normal muscle at a given submaximal
dose of release trigger. This difference is observed when the channel is
stimulated directly by polylysine or indirectly via a
depolarization-induced activation of the T-tubule dihydropyridine receptor.</description><identifier>ISSN: 0363-6143</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.1995.268.6.c1381</identifier><identifier>PMID: 7611356</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Calcium - metabolism ; Disease Susceptibility ; Kinetics ; Malignant Hyperthermia - physiopathology ; Malignant Hyperthermia - veterinary ; Membrane Potentials ; Microsomes - drug effects ; Microsomes - metabolism ; Muscle Contraction ; Muscle, Skeletal - physiology ; Muscle, Skeletal - physiopathology ; Polylysine - pharmacology ; Reference Values ; Sarcoplasmic Reticulum - drug effects ; Sarcoplasmic Reticulum - metabolism ; Swine ; Swine Diseases ; Time Factors</subject><ispartof>American Journal of Physiology: Cell Physiology, 1995-06, Vol.268 (6), p.C1381-C1386</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-d4907f9bba34e4a436c352014ee4002a90f96f3c35fb76bc62b1b6d43de3b3573</citedby><cites>FETCH-LOGICAL-c349t-d4907f9bba34e4a436c352014ee4002a90f96f3c35fb76bc62b1b6d43de3b3573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7611356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>el-Hayek, R</creatorcontrib><creatorcontrib>Yano, M</creatorcontrib><creatorcontrib>Antoniu, B</creatorcontrib><creatorcontrib>Mickelson, J. R</creatorcontrib><creatorcontrib>Louis, C. F</creatorcontrib><creatorcontrib>Ikemoto, N</creatorcontrib><title>Altered E-C coupling in triads isolated from malignant hyperthermia-susceptible porcine muscle</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol</addtitle><description>R. el-Hayek, M. Yano, B. Antoniu, J. R. Mickelson, C. F. Louis and N. Ikemoto
Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA.
Triad vesicles were isolated from normal (N) and homozygous malignant
hyperthermia-susceptible (MHS) porcine skeletal muscle, and two types of
sarcoplasmic reticulum Ca2+ release were investigated: 1)
polylysine-induced Ca2+ release (direct stimulation of the junctional foot
protein), and 2) depolarization-induced Ca2+ release (stimulation of the
junctional foot protein via the dihydropyridine receptor). At submaximal
concentrations of polylysine, the rates of induced Ca2+ release from the
MHS triads were greater than from normal triads. The T tubules of polarized
triads were depolarized by the K(+)-to-Na+ ionic replacement protocol.
Higher grades of T-tubule depolarization resulted in higher rates of Ca2+
release from both MHS and normal triads but, when compared at a given grade
of T-tubule depolarization, the release rate was always greater from the
MHS than from normal triads. Thus the activity of the SR Ca2+ release
channel is always higher in MHS than in normal muscle at a given submaximal
dose of release trigger. This difference is observed when the channel is
stimulated directly by polylysine or indirectly via a
depolarization-induced activation of the T-tubule dihydropyridine receptor.</description><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Disease Susceptibility</subject><subject>Kinetics</subject><subject>Malignant Hyperthermia - physiopathology</subject><subject>Malignant Hyperthermia - veterinary</subject><subject>Membrane Potentials</subject><subject>Microsomes - drug effects</subject><subject>Microsomes - metabolism</subject><subject>Muscle Contraction</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscle, Skeletal - physiopathology</subject><subject>Polylysine - pharmacology</subject><subject>Reference Values</subject><subject>Sarcoplasmic Reticulum - drug effects</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Swine</subject><subject>Swine Diseases</subject><subject>Time Factors</subject><issn>0363-6143</issn><issn>0002-9513</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkF9LwzAUxYMoc04_gpAn31qTJk3XxzHmHxB80VdDkt6uGWlTkxbZt7dzY_h04Zx7zuX-EMKUpJTm2aPa9QacS2lZ5mkmlqlIDWVLeoHmk50lNBfsEs0JEywRlLNrdBPjjhDCM1HO0KwQlLJczNHXyg0QoMKbZI2NH3tnuy22HR6CVVXENnqnhsmvg29xq5zddqobcLPvIQwNhNaqJI7RQD9Y7QD3PhjbAW4nzcEtuqqVi3B3mgv0-bT5WL8kb-_Pr-vVW2IYL4ek4iUp6lJrxThwxZkwLM8I5QCckEyVpC5FzSax1oXQRmSaalFxVgHTLC_YAj0ce_vgv0eIg2xtPBBSHfgxyqLgf89Pi8vjogk-xgC17INtVdhLSuQBrTyhlQe0ckIrhVwf0E7R-9ONUbdQnYMnlpOfHv3GbpsfG0D2zT5a7_x2f279X_gLtAGJBA</recordid><startdate>19950601</startdate><enddate>19950601</enddate><creator>el-Hayek, R</creator><creator>Yano, M</creator><creator>Antoniu, B</creator><creator>Mickelson, J. R</creator><creator>Louis, C. F</creator><creator>Ikemoto, N</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950601</creationdate><title>Altered E-C coupling in triads isolated from malignant hyperthermia-susceptible porcine muscle</title><author>el-Hayek, R ; Yano, M ; Antoniu, B ; Mickelson, J. R ; Louis, C. F ; Ikemoto, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-d4907f9bba34e4a436c352014ee4002a90f96f3c35fb76bc62b1b6d43de3b3573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Disease Susceptibility</topic><topic>Kinetics</topic><topic>Malignant Hyperthermia - physiopathology</topic><topic>Malignant Hyperthermia - veterinary</topic><topic>Membrane Potentials</topic><topic>Microsomes - drug effects</topic><topic>Microsomes - metabolism</topic><topic>Muscle Contraction</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscle, Skeletal - physiopathology</topic><topic>Polylysine - pharmacology</topic><topic>Reference Values</topic><topic>Sarcoplasmic Reticulum - drug effects</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Swine</topic><topic>Swine Diseases</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>el-Hayek, R</creatorcontrib><creatorcontrib>Yano, M</creatorcontrib><creatorcontrib>Antoniu, B</creatorcontrib><creatorcontrib>Mickelson, J. R</creatorcontrib><creatorcontrib>Louis, C. F</creatorcontrib><creatorcontrib>Ikemoto, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>el-Hayek, R</au><au>Yano, M</au><au>Antoniu, B</au><au>Mickelson, J. R</au><au>Louis, C. F</au><au>Ikemoto, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered E-C coupling in triads isolated from malignant hyperthermia-susceptible porcine muscle</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1995-06-01</date><risdate>1995</risdate><volume>268</volume><issue>6</issue><spage>C1381</spage><epage>C1386</epage><pages>C1381-C1386</pages><issn>0363-6143</issn><issn>0002-9513</issn><eissn>1522-1563</eissn><abstract>R. el-Hayek, M. Yano, B. Antoniu, J. R. Mickelson, C. F. Louis and N. Ikemoto
Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA.
Triad vesicles were isolated from normal (N) and homozygous malignant
hyperthermia-susceptible (MHS) porcine skeletal muscle, and two types of
sarcoplasmic reticulum Ca2+ release were investigated: 1)
polylysine-induced Ca2+ release (direct stimulation of the junctional foot
protein), and 2) depolarization-induced Ca2+ release (stimulation of the
junctional foot protein via the dihydropyridine receptor). At submaximal
concentrations of polylysine, the rates of induced Ca2+ release from the
MHS triads were greater than from normal triads. The T tubules of polarized
triads were depolarized by the K(+)-to-Na+ ionic replacement protocol.
Higher grades of T-tubule depolarization resulted in higher rates of Ca2+
release from both MHS and normal triads but, when compared at a given grade
of T-tubule depolarization, the release rate was always greater from the
MHS than from normal triads. Thus the activity of the SR Ca2+ release
channel is always higher in MHS than in normal muscle at a given submaximal
dose of release trigger. This difference is observed when the channel is
stimulated directly by polylysine or indirectly via a
depolarization-induced activation of the T-tubule dihydropyridine receptor.</abstract><cop>United States</cop><pmid>7611356</pmid><doi>10.1152/ajpcell.1995.268.6.c1381</doi></addata></record> |
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| ispartof | American Journal of Physiology: Cell Physiology, 1995-06, Vol.268 (6), p.C1381-C1386 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Calcium - metabolism Disease Susceptibility Kinetics Malignant Hyperthermia - physiopathology Malignant Hyperthermia - veterinary Membrane Potentials Microsomes - drug effects Microsomes - metabolism Muscle Contraction Muscle, Skeletal - physiology Muscle, Skeletal - physiopathology Polylysine - pharmacology Reference Values Sarcoplasmic Reticulum - drug effects Sarcoplasmic Reticulum - metabolism Swine Swine Diseases Time Factors |
| title | Altered E-C coupling in triads isolated from malignant hyperthermia-susceptible porcine muscle |
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