Effect of morphine on mesangial immunoglobulin G aggregate kinetics
P. C. Singhal, C. Q. Pan, N. Gibbons and E. Valderrama Department of Medicine and Pathology, Long Island Jewish Medical Center, New Hyde Park, New York 11042. Because mesangial expansion is considered a precursor of focal glomerulosclerosis, we studied whether morphine can cause mesangial expansion....
Gespeichert in:
| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 1993-11, Vol.265 (5), p.C1211-C1219 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | C1219 |
|---|---|
| container_issue | 5 |
| container_start_page | C1211 |
| container_title | American Journal of Physiology: Cell Physiology |
| container_volume | 265 |
| creator | Singhal, P. C Pan, C. Q Gibbons, N Valderrama, E |
| description | P. C. Singhal, C. Q. Pan, N. Gibbons and E. Valderrama
Department of Medicine and Pathology, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
Because mesangial expansion is considered a precursor of focal
glomerulosclerosis, we studied whether morphine can cause mesangial
expansion. We used radiolabeled human immunoglobulin G aggregates
(125I-ahIgG) to study mesangial kinetics in control and experimental
(morphine-treated) rats. Control and experimental rats were administered
125I-ahIgG by tail vein. Serum levels of 125I-ahIgG and uptake of
125I-ahIgG by liver, spleen, and mesangium were determined at 4, 8, 12, 24,
and 36 h after 125I-ahIgG administration. Mesangial 125I-ahIgG levels were
higher (P < 0.05) at 4 h and at later periods in morphine-treated vs.
control rats. Naloxone, an opioid antagonist, did not attenuate the
morphine-induced mesangial accumulation of 125I-ahIgG. The mean uptake of
IgG aggregates was lower in the liver and spleen of morphine-treated rats
at 36 h (P < 0.05). In both in vivo and in vitro experiments,
ultrastructural studies showed accumulation of IgG-coated gold particles in
vesicles, endosomes, and lysosomes. Morphine may have increased the
accumulation of 125I-ahIgG in the glomeruli either by increasing the
delivery of macromolecules into the mesangium or by altering the exit of
macromolecules from the mesangium. |
| doi_str_mv | 10.1152/ajpcell.1993.265.5.c1211 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_highwire_physiology_ajpcell_265_5_C1211</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76083163</sourcerecordid><originalsourceid>FETCH-LOGICAL-c483t-86e0c28e5b32961a119ee211395eaacce5252b847e3060abff3458378aa53e913</originalsourceid><addsrcrecordid>eNpNkMtOhDAUhhujGcfRRzDpyh3YC-2UpSE6mkziRtdNqQfoCBQpxMzbCxkycXUW_-X8-RDClMSUCvZoDp2Fuo5pmvKYSRGL2FJG6QVaTzKLqJD8Eq0JlzySNOHX6CaEAyEkYTJdoZViXCXbZI2y56IAO2Bf4Mb3XeVawL7FDQTTls7U2DXN2Pqy9vlYuxbvsCnLHkozAP6ezIOz4RZdFaYOcLfcDfp8ef7IXqP9--4te9pHNlF8iJQEYpkCkXOWSmooTQGmzTwVYIy1IJhg-TQLOJHE5EXBE6H4VhkjOKSUb9DDqbfr_c8IYdCNCzMG04Ifg95KojiVfDKqk9H2PoQeCt31rjH9UVOiZ3564adnfnrip4XOZn5T9H75MeYNfJ2DC7BJj0965crq1_Wgu-oYnK99eTy3_i_8A_G6fjg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76083163</pqid></control><display><type>article</type><title>Effect of morphine on mesangial immunoglobulin G aggregate kinetics</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Singhal, P. C ; Pan, C. Q ; Gibbons, N ; Valderrama, E</creator><creatorcontrib>Singhal, P. C ; Pan, C. Q ; Gibbons, N ; Valderrama, E</creatorcontrib><description>P. C. Singhal, C. Q. Pan, N. Gibbons and E. Valderrama
Department of Medicine and Pathology, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
Because mesangial expansion is considered a precursor of focal
glomerulosclerosis, we studied whether morphine can cause mesangial
expansion. We used radiolabeled human immunoglobulin G aggregates
(125I-ahIgG) to study mesangial kinetics in control and experimental
(morphine-treated) rats. Control and experimental rats were administered
125I-ahIgG by tail vein. Serum levels of 125I-ahIgG and uptake of
125I-ahIgG by liver, spleen, and mesangium were determined at 4, 8, 12, 24,
and 36 h after 125I-ahIgG administration. Mesangial 125I-ahIgG levels were
higher (P < 0.05) at 4 h and at later periods in morphine-treated vs.
control rats. Naloxone, an opioid antagonist, did not attenuate the
morphine-induced mesangial accumulation of 125I-ahIgG. The mean uptake of
IgG aggregates was lower in the liver and spleen of morphine-treated rats
at 36 h (P < 0.05). In both in vivo and in vitro experiments,
ultrastructural studies showed accumulation of IgG-coated gold particles in
vesicles, endosomes, and lysosomes. Morphine may have increased the
accumulation of 125I-ahIgG in the glomeruli either by increasing the
delivery of macromolecules into the mesangium or by altering the exit of
macromolecules from the mesangium.</description><identifier>ISSN: 0363-6143</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.1993.265.5.c1211</identifier><identifier>PMID: 8238474</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Biological Transport - drug effects ; Cells, Cultured ; Dogs ; Glomerular Mesangium - drug effects ; Glomerular Mesangium - physiology ; Glomerular Mesangium - ultrastructure ; Humans ; Immunoglobulin G - blood ; Immunoglobulin G - drug effects ; Immunoglobulin G - metabolism ; Iodine Radioisotopes ; Kinetics ; Liver - metabolism ; Lung - metabolism ; Macrophages - drug effects ; Macrophages - physiology ; Macrophages - ultrastructure ; Male ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Morphine - pharmacology ; Naloxone - pharmacology ; Phagocytosis - drug effects ; Rats ; Rats, Sprague-Dawley ; Spleen - metabolism ; Tissue Distribution</subject><ispartof>American Journal of Physiology: Cell Physiology, 1993-11, Vol.265 (5), p.C1211-C1219</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-86e0c28e5b32961a119ee211395eaacce5252b847e3060abff3458378aa53e913</citedby><cites>FETCH-LOGICAL-c483t-86e0c28e5b32961a119ee211395eaacce5252b847e3060abff3458378aa53e913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8238474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singhal, P. C</creatorcontrib><creatorcontrib>Pan, C. Q</creatorcontrib><creatorcontrib>Gibbons, N</creatorcontrib><creatorcontrib>Valderrama, E</creatorcontrib><title>Effect of morphine on mesangial immunoglobulin G aggregate kinetics</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol</addtitle><description>P. C. Singhal, C. Q. Pan, N. Gibbons and E. Valderrama
Department of Medicine and Pathology, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
Because mesangial expansion is considered a precursor of focal
glomerulosclerosis, we studied whether morphine can cause mesangial
expansion. We used radiolabeled human immunoglobulin G aggregates
(125I-ahIgG) to study mesangial kinetics in control and experimental
(morphine-treated) rats. Control and experimental rats were administered
125I-ahIgG by tail vein. Serum levels of 125I-ahIgG and uptake of
125I-ahIgG by liver, spleen, and mesangium were determined at 4, 8, 12, 24,
and 36 h after 125I-ahIgG administration. Mesangial 125I-ahIgG levels were
higher (P < 0.05) at 4 h and at later periods in morphine-treated vs.
control rats. Naloxone, an opioid antagonist, did not attenuate the
morphine-induced mesangial accumulation of 125I-ahIgG. The mean uptake of
IgG aggregates was lower in the liver and spleen of morphine-treated rats
at 36 h (P < 0.05). In both in vivo and in vitro experiments,
ultrastructural studies showed accumulation of IgG-coated gold particles in
vesicles, endosomes, and lysosomes. Morphine may have increased the
accumulation of 125I-ahIgG in the glomeruli either by increasing the
delivery of macromolecules into the mesangium or by altering the exit of
macromolecules from the mesangium.</description><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Cells, Cultured</subject><subject>Dogs</subject><subject>Glomerular Mesangium - drug effects</subject><subject>Glomerular Mesangium - physiology</subject><subject>Glomerular Mesangium - ultrastructure</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - drug effects</subject><subject>Immunoglobulin G - metabolism</subject><subject>Iodine Radioisotopes</subject><subject>Kinetics</subject><subject>Liver - metabolism</subject><subject>Lung - metabolism</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - physiology</subject><subject>Macrophages - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Morphine - pharmacology</subject><subject>Naloxone - pharmacology</subject><subject>Phagocytosis - drug effects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spleen - metabolism</subject><subject>Tissue Distribution</subject><issn>0363-6143</issn><issn>0002-9513</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMtOhDAUhhujGcfRRzDpyh3YC-2UpSE6mkziRtdNqQfoCBQpxMzbCxkycXUW_-X8-RDClMSUCvZoDp2Fuo5pmvKYSRGL2FJG6QVaTzKLqJD8Eq0JlzySNOHX6CaEAyEkYTJdoZViXCXbZI2y56IAO2Bf4Mb3XeVawL7FDQTTls7U2DXN2Pqy9vlYuxbvsCnLHkozAP6ezIOz4RZdFaYOcLfcDfp8ef7IXqP9--4te9pHNlF8iJQEYpkCkXOWSmooTQGmzTwVYIy1IJhg-TQLOJHE5EXBE6H4VhkjOKSUb9DDqbfr_c8IYdCNCzMG04Ifg95KojiVfDKqk9H2PoQeCt31rjH9UVOiZ3564adnfnrip4XOZn5T9H75MeYNfJ2DC7BJj0965crq1_Wgu-oYnK99eTy3_i_8A_G6fjg</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Singhal, P. C</creator><creator>Pan, C. Q</creator><creator>Gibbons, N</creator><creator>Valderrama, E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Effect of morphine on mesangial immunoglobulin G aggregate kinetics</title><author>Singhal, P. C ; Pan, C. Q ; Gibbons, N ; Valderrama, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-86e0c28e5b32961a119ee211395eaacce5252b847e3060abff3458378aa53e913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Cells, Cultured</topic><topic>Dogs</topic><topic>Glomerular Mesangium - drug effects</topic><topic>Glomerular Mesangium - physiology</topic><topic>Glomerular Mesangium - ultrastructure</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - drug effects</topic><topic>Immunoglobulin G - metabolism</topic><topic>Iodine Radioisotopes</topic><topic>Kinetics</topic><topic>Liver - metabolism</topic><topic>Lung - metabolism</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - physiology</topic><topic>Macrophages - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Morphine - pharmacology</topic><topic>Naloxone - pharmacology</topic><topic>Phagocytosis - drug effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spleen - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singhal, P. C</creatorcontrib><creatorcontrib>Pan, C. Q</creatorcontrib><creatorcontrib>Gibbons, N</creatorcontrib><creatorcontrib>Valderrama, E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singhal, P. C</au><au>Pan, C. Q</au><au>Gibbons, N</au><au>Valderrama, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of morphine on mesangial immunoglobulin G aggregate kinetics</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>265</volume><issue>5</issue><spage>C1211</spage><epage>C1219</epage><pages>C1211-C1219</pages><issn>0363-6143</issn><issn>0002-9513</issn><eissn>1522-1563</eissn><abstract>P. C. Singhal, C. Q. Pan, N. Gibbons and E. Valderrama
Department of Medicine and Pathology, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
Because mesangial expansion is considered a precursor of focal
glomerulosclerosis, we studied whether morphine can cause mesangial
expansion. We used radiolabeled human immunoglobulin G aggregates
(125I-ahIgG) to study mesangial kinetics in control and experimental
(morphine-treated) rats. Control and experimental rats were administered
125I-ahIgG by tail vein. Serum levels of 125I-ahIgG and uptake of
125I-ahIgG by liver, spleen, and mesangium were determined at 4, 8, 12, 24,
and 36 h after 125I-ahIgG administration. Mesangial 125I-ahIgG levels were
higher (P < 0.05) at 4 h and at later periods in morphine-treated vs.
control rats. Naloxone, an opioid antagonist, did not attenuate the
morphine-induced mesangial accumulation of 125I-ahIgG. The mean uptake of
IgG aggregates was lower in the liver and spleen of morphine-treated rats
at 36 h (P < 0.05). In both in vivo and in vitro experiments,
ultrastructural studies showed accumulation of IgG-coated gold particles in
vesicles, endosomes, and lysosomes. Morphine may have increased the
accumulation of 125I-ahIgG in the glomeruli either by increasing the
delivery of macromolecules into the mesangium or by altering the exit of
macromolecules from the mesangium.</abstract><cop>United States</cop><pmid>8238474</pmid><doi>10.1152/ajpcell.1993.265.5.c1211</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 1993-11, Vol.265 (5), p.C1211-C1219 |
| issn | 0363-6143 0002-9513 1522-1563 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpcell_265_5_C1211 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological Transport - drug effects Cells, Cultured Dogs Glomerular Mesangium - drug effects Glomerular Mesangium - physiology Glomerular Mesangium - ultrastructure Humans Immunoglobulin G - blood Immunoglobulin G - drug effects Immunoglobulin G - metabolism Iodine Radioisotopes Kinetics Liver - metabolism Lung - metabolism Macrophages - drug effects Macrophages - physiology Macrophages - ultrastructure Male Microscopy, Electron Microscopy, Electron, Scanning Morphine - pharmacology Naloxone - pharmacology Phagocytosis - drug effects Rats Rats, Sprague-Dawley Spleen - metabolism Tissue Distribution |
| title | Effect of morphine on mesangial immunoglobulin G aggregate kinetics |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T22%3A58%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20morphine%20on%20mesangial%20immunoglobulin%20G%20aggregate%20kinetics&rft.jtitle=American%20Journal%20of%20Physiology:%20Cell%20Physiology&rft.au=Singhal,%20P.%20C&rft.date=1993-11-01&rft.volume=265&rft.issue=5&rft.spage=C1211&rft.epage=C1219&rft.pages=C1211-C1219&rft.issn=0363-6143&rft.eissn=1522-1563&rft_id=info:doi/10.1152/ajpcell.1993.265.5.c1211&rft_dat=%3Cproquest_pubme%3E76083163%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76083163&rft_id=info:pmid/8238474&rfr_iscdi=true |