Direct Interaction in T-cells between θPKC and the Tyrosine Kinase p59fyn

The protein kinase C (PKC) family has been clearly implicated in T-cell activation as have several nonreceptor protein-tyrosine kinases associated with the T-cell receptor, including p59fyn. This report demonstrates that θPKC and p59fyn specifically interact in vitro , in the yeast two-hybrid syste...

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Veröffentlicht in:The Journal of biological chemistry 1999-07, Vol.274 (27), p.19003
Hauptverfasser: Dorit Ron, Eugene W. Napolitano, Anna Voronova, Nicki J. Vasquez, Doug N. Roberts, Brenda L. Calio, Roger H. Caothien, Sherrie M. Pettiford, Sarah Wellik, Janis B. Mandac, Lawrence M. Kauvar
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container_end_page
container_issue 27
container_start_page 19003
container_title The Journal of biological chemistry
container_volume 274
creator Dorit Ron
Eugene W. Napolitano
Anna Voronova
Nicki J. Vasquez
Doug N. Roberts
Brenda L. Calio
Roger H. Caothien
Sherrie M. Pettiford
Sarah Wellik
Janis B. Mandac
Lawrence M. Kauvar
description The protein kinase C (PKC) family has been clearly implicated in T-cell activation as have several nonreceptor protein-tyrosine kinases associated with the T-cell receptor, including p59fyn. This report demonstrates that θPKC and p59fyn specifically interact in vitro , in the yeast two-hybrid system, and in T-cells. Further indications of direct interaction are that p59fyn potentiates θPKC catalytic activity and that θPKC is a substrate for tyrosine phosphorylation by p59fyn. This interaction may account for the localization of θPKC following T-cell activation, pharmacological disruption of which results in specific cell-signaling defects. The demonstration of a physical interaction between a PKC and a protein-tyrosine kinase expands the class of PKC-anchoring proteins (receptors for activated C kinases (RACKs)) and demonstrates a direct connection between these two major T-cell-signaling pathways.
doi_str_mv 10.1074/jbc.274.27.19003
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title Direct Interaction in T-cells between θPKC and the Tyrosine Kinase p59fyn
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