Characterization of a Single-chain Antibody to the -Chain of the T Cell Receptor

In this report the V and V genes of the anti-T cell receptor (TCR) antibody KJ16, which recognizes the TCR Vβ8.1 and Vβ8.2 regions in mice, were cloned and expressed as a single-chain antibody (scFv) in Escherichia coli . A 29-kDa protein was obtained after renaturation from inclusion bodies. The...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of biological chemistry 1995-10, Vol.270 (43), p.25819
Hauptverfasser: Bryan K. Cho, Beth A. Schodin, David M. Kranz
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!