Rapid Detection and Molecular Serotyping of Adenovirus by Use of PCR Followed by Electrospray Ionization Mass Spectrometry

We have developed a PCR/electrospray ionization mass spectrometry (PCR/ESI-MS) assay for the rapid detection, identification, and serotyping of human adenoviruses. The assay employs a high-performance mass spectrometer to "weigh" the amplicons obtained from PCR using primers designed to am...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-02, Vol.46 (2), p.644-651
Hauptverfasser:	Blyn, Lawrence B, Hall, Thomas A, Libby, Brian, Ranken, Raymond, Sampath, Rangarajan, Rudnick, Karl, Moradi, Emily, Desai, Anjali, Metzgar, David, Russell, Kevin L, Freed, Nikki E, Balansay, Melinda, Broderick, Michael P, Osuna, Miguel A, Hofstadler, Steven A, Ecker, David J
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - classification Adenoviridae - genetics Adenoviridae - isolation & purification Adenoviridae Infections - diagnosis Adenoviridae Infections - virology Adenovirus Automatic Data Processing Biological and medical sciences Chlamydiales DNA Primers - genetics Environmental Microbiology Fundamental and applied biological sciences. Psychology Humans Microbiology Miscellaneous Polymerase Chain Reaction - methods Sensitivity and Specificity Serotyping - methods Spectrometry, Mass, Electrospray Ionization - methods Virology
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