Detection of Cariogenic Streptococcus mutans in Extirpated Heart Valve and Atheromatous Plaque Specimens
The involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases....
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| Veröffentlicht in: | Journal of Clinical Microbiology 2006-09, Vol.44 (9), p.3313-3317 |
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| creator | Nakano, Kazuhiko Inaba, Hiroaki Nomura, Ryota Nemoto, Hirotoshi Takeda, Munehiro Yoshioka, Hideo Matsue, Hajime Takahashi, Toshiki Taniguchi, Kazuhiro Amano, Atsuo Ooshima, Takashi |
| description | The involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. However, no information is available regarding the involvement of cariogenic pathogens such as Streptococcus mutans. The presence of oral streptococcal species and periodontitis-related bacteria in 35 heart valve and 27 atheromatous plaque clinical specimens, as well as 32 dental plaque specimens from the same subjects, was analyzed using a PCR method. Furthermore, broad-range PCR with DNA sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans was frequently detected in the heart valve (69%) and atheromatous plaque (74%) specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. The bacterial composition in cardiovascular tissues was found to be markedly distinct from that in dental plaque, with only a limited number of species, including S. mutans, in the cardiovascular regions shown to have possibly originated from the oral cavity. Semiquantitative assay results revealed that S. mutans was detected in significant quantities in the heart valve (40%) and atheromatous plaque (48%) specimens, whereas the quantities of all other tested bacterial species, including several related to periodontitis, were negligible in the cardiovascular samples. These results indicate that S. mutans is a possible causative agent of cardiovascular disease. |
| doi_str_mv | 10.1128/JCM.00377-06 |
| format | Article |
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However, no information is available regarding the involvement of cariogenic pathogens such as Streptococcus mutans. The presence of oral streptococcal species and periodontitis-related bacteria in 35 heart valve and 27 atheromatous plaque clinical specimens, as well as 32 dental plaque specimens from the same subjects, was analyzed using a PCR method. Furthermore, broad-range PCR with DNA sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans was frequently detected in the heart valve (69%) and atheromatous plaque (74%) specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. The bacterial composition in cardiovascular tissues was found to be markedly distinct from that in dental plaque, with only a limited number of species, including S. mutans, in the cardiovascular regions shown to have possibly originated from the oral cavity. Semiquantitative assay results revealed that S. mutans was detected in significant quantities in the heart valve (40%) and atheromatous plaque (48%) specimens, whereas the quantities of all other tested bacterial species, including several related to periodontitis, were negligible in the cardiovascular samples. These results indicate that S. mutans is a possible causative agent of cardiovascular disease.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JCM.00377-06</identifier><identifier>PMID: 16954266</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Aged ; Aged, 80 and over ; Atherosclerosis - microbiology ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteriology ; Biological and medical sciences ; Dental Caries - microbiology ; Dental Plaque - microbiology ; DNA, Bacterial - analysis ; DNA, Bacterial - isolation & purification ; Female ; Fundamental and applied biological sciences. Psychology ; Heart Valve Diseases - microbiology ; Heart Valves - microbiology ; Humans ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Periodontitis - microbiology ; Polymerase Chain Reaction - methods ; Sequence Analysis, DNA ; Streptococcal Infections - microbiology ; Streptococcus ; Streptococcus mutans ; Streptococcus mutans - classification ; Streptococcus mutans - genetics ; Streptococcus mutans - isolation & purification</subject><ispartof>Journal of Clinical Microbiology, 2006-09, Vol.44 (9), p.3313-3317</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-4baffab7b322d4d323dca337bdd629f7baa7d958bcd596dc5e8d738af0c74a513</citedby><cites>FETCH-LOGICAL-c536t-4baffab7b322d4d323dca337bdd629f7baa7d958bcd596dc5e8d738af0c74a513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594668/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594668/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18099065$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16954266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakano, Kazuhiko</creatorcontrib><creatorcontrib>Inaba, Hiroaki</creatorcontrib><creatorcontrib>Nomura, Ryota</creatorcontrib><creatorcontrib>Nemoto, Hirotoshi</creatorcontrib><creatorcontrib>Takeda, Munehiro</creatorcontrib><creatorcontrib>Yoshioka, Hideo</creatorcontrib><creatorcontrib>Matsue, Hajime</creatorcontrib><creatorcontrib>Takahashi, Toshiki</creatorcontrib><creatorcontrib>Taniguchi, Kazuhiro</creatorcontrib><creatorcontrib>Amano, Atsuo</creatorcontrib><creatorcontrib>Ooshima, Takashi</creatorcontrib><title>Detection of Cariogenic Streptococcus mutans in Extirpated Heart Valve and Atheromatous Plaque Specimens</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. However, no information is available regarding the involvement of cariogenic pathogens such as Streptococcus mutans. The presence of oral streptococcal species and periodontitis-related bacteria in 35 heart valve and 27 atheromatous plaque clinical specimens, as well as 32 dental plaque specimens from the same subjects, was analyzed using a PCR method. Furthermore, broad-range PCR with DNA sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans was frequently detected in the heart valve (69%) and atheromatous plaque (74%) specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. The bacterial composition in cardiovascular tissues was found to be markedly distinct from that in dental plaque, with only a limited number of species, including S. mutans, in the cardiovascular regions shown to have possibly originated from the oral cavity. Semiquantitative assay results revealed that S. mutans was detected in significant quantities in the heart valve (40%) and atheromatous plaque (48%) specimens, whereas the quantities of all other tested bacterial species, including several related to periodontitis, were negligible in the cardiovascular samples. These results indicate that S. mutans is a possible causative agent of cardiovascular disease.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Atherosclerosis - microbiology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Dental Caries - microbiology</subject><subject>Dental Plaque - microbiology</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart Valve Diseases - microbiology</subject><subject>Heart Valves - microbiology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Periodontitis - microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sequence Analysis, DNA</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcus</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - classification</subject><subject>Streptococcus mutans - genetics</subject><subject>Streptococcus mutans - isolation & purification</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0b9v1DAcBfAIgehR2JjBDDCRYse_l0rVtVBQEUhHEZv1je3cuUriYPta-O9JuROFicmDP37y06uqpwQfEdKoNx-WH48wplLWWNyrFgRrVQuBv92vFhhrXhNC5UH1KOcrjAljnD-sDojQnDVCLKrNqS_elhBHFDu0hBTi2o_BolVJfirRRmu3GQ3bAmNGYURnP0pIExTv0LmHVNBX6K89gtGhk7LxKQ5Q4vzicw_ftx6tJm_D4Mf8uHrQQZ_9k_15WF2-PfuyPK8vPr17vzy5qC2notSsha6DVra0aRxztKHOAqWydU40upMtgHSaq9Y6roWz3CsnqYIOW8mAE3pYHe9yp207eGf9WBL0ZkphgPTTRAjm35sxbMw6XhvCNRNCzQGv9gEpzg1yMUPI1vc9jH4uZoRSlEhF_wuJpkQLpmf4egdtijkn3_35DcHmdkMzb2h-b2iwmPmzvxvc4f1oM3i5B5At9F2C0YZ85xTWGgs-uxc7twnrzU1I3kAezJUdDGNGG0rJbYnnO9NBNLBOc87lqsGEYkIwUZrSXzcGu9Q</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Nakano, Kazuhiko</creator><creator>Inaba, Hiroaki</creator><creator>Nomura, Ryota</creator><creator>Nemoto, Hirotoshi</creator><creator>Takeda, Munehiro</creator><creator>Yoshioka, Hideo</creator><creator>Matsue, Hajime</creator><creator>Takahashi, Toshiki</creator><creator>Taniguchi, Kazuhiro</creator><creator>Amano, Atsuo</creator><creator>Ooshima, Takashi</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060901</creationdate><title>Detection of Cariogenic Streptococcus mutans in Extirpated Heart Valve and Atheromatous Plaque Specimens</title><author>Nakano, Kazuhiko ; Inaba, Hiroaki ; Nomura, Ryota ; Nemoto, Hirotoshi ; Takeda, Munehiro ; Yoshioka, Hideo ; Matsue, Hajime ; Takahashi, Toshiki ; Taniguchi, Kazuhiro ; Amano, Atsuo ; Ooshima, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-4baffab7b322d4d323dca337bdd629f7baa7d958bcd596dc5e8d738af0c74a513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Atherosclerosis - microbiology</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Dental Caries - microbiology</topic><topic>Dental Plaque - microbiology</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart Valve Diseases - microbiology</topic><topic>Heart Valves - microbiology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Periodontitis - microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sequence Analysis, DNA</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - classification</topic><topic>Streptococcus mutans - genetics</topic><topic>Streptococcus mutans - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakano, Kazuhiko</creatorcontrib><creatorcontrib>Inaba, Hiroaki</creatorcontrib><creatorcontrib>Nomura, Ryota</creatorcontrib><creatorcontrib>Nemoto, Hirotoshi</creatorcontrib><creatorcontrib>Takeda, Munehiro</creatorcontrib><creatorcontrib>Yoshioka, Hideo</creatorcontrib><creatorcontrib>Matsue, Hajime</creatorcontrib><creatorcontrib>Takahashi, Toshiki</creatorcontrib><creatorcontrib>Taniguchi, Kazuhiro</creatorcontrib><creatorcontrib>Amano, Atsuo</creatorcontrib><creatorcontrib>Ooshima, Takashi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakano, Kazuhiko</au><au>Inaba, Hiroaki</au><au>Nomura, Ryota</au><au>Nemoto, Hirotoshi</au><au>Takeda, Munehiro</au><au>Yoshioka, Hideo</au><au>Matsue, Hajime</au><au>Takahashi, Toshiki</au><au>Taniguchi, Kazuhiro</au><au>Amano, Atsuo</au><au>Ooshima, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Cariogenic Streptococcus mutans in Extirpated Heart Valve and Atheromatous Plaque Specimens</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>44</volume><issue>9</issue><spage>3313</spage><epage>3317</epage><pages>3313-3317</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>The involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. However, no information is available regarding the involvement of cariogenic pathogens such as Streptococcus mutans. The presence of oral streptococcal species and periodontitis-related bacteria in 35 heart valve and 27 atheromatous plaque clinical specimens, as well as 32 dental plaque specimens from the same subjects, was analyzed using a PCR method. Furthermore, broad-range PCR with DNA sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans was frequently detected in the heart valve (69%) and atheromatous plaque (74%) specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. The bacterial composition in cardiovascular tissues was found to be markedly distinct from that in dental plaque, with only a limited number of species, including S. mutans, in the cardiovascular regions shown to have possibly originated from the oral cavity. Semiquantitative assay results revealed that S. mutans was detected in significant quantities in the heart valve (40%) and atheromatous plaque (48%) specimens, whereas the quantities of all other tested bacterial species, including several related to periodontitis, were negligible in the cardiovascular samples. These results indicate that S. mutans is a possible causative agent of cardiovascular disease.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16954266</pmid><doi>10.1128/JCM.00377-06</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Aged, 80 and over Atherosclerosis - microbiology Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteriology Biological and medical sciences Dental Caries - microbiology Dental Plaque - microbiology DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Female Fundamental and applied biological sciences. Psychology Heart Valve Diseases - microbiology Heart Valves - microbiology Humans Infectious diseases Male Medical sciences Microbiology Middle Aged Miscellaneous Periodontitis - microbiology Polymerase Chain Reaction - methods Sequence Analysis, DNA Streptococcal Infections - microbiology Streptococcus Streptococcus mutans Streptococcus mutans - classification Streptococcus mutans - genetics Streptococcus mutans - isolation & purification |
| title | Detection of Cariogenic Streptococcus mutans in Extirpated Heart Valve and Atheromatous Plaque Specimens |
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