Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures
We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. al...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2005-06, Vol.43 (6), p.2909-2912 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 43 |
| creator | Wilson, D. A Joyce, M. J Hall, L. S Reller, L. B Roberts, G. D Hall, G. S Alexander, B. D Procop, G. W |
| description | We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively. |
| doi_str_mv | 10.1128/JCM.43.6.2909-2912.2005 |
| format | Article |
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A ; Joyce, M. J ; Hall, L. S ; Reller, L. B ; Roberts, G. D ; Hall, G. S ; Alexander, B. D ; Procop, G. W</creator><creatorcontrib>Wilson, D. A ; Joyce, M. J ; Hall, L. S ; Reller, L. B ; Roberts, G. D ; Hall, G. S ; Alexander, B. D ; Procop, G. W</creatorcontrib><description>We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.43.6.2909-2912.2005</identifier><identifier>PMID: 15956416</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacterial diseases ; Bacterial sepsis ; Biological and medical sciences ; Blood - microbiology ; Candida albicans ; Candida albicans - classification ; Candida albicans - genetics ; Candida albicans - isolation & purification ; Candidiasis - microbiology ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Fungemia - microbiology ; Human bacterial diseases ; Humans ; In Situ Hybridization, Fluorescence - methods ; Infectious diseases ; Medical sciences ; Microbiology ; Miscellaneous ; Mycology ; Nucleic Acid Probes - genetics ; Peptide Nucleic Acids - genetics ; Predictive Value of Tests ; Sensitivity and Specificity</subject><ispartof>Journal of Clinical Microbiology, 2005-06, Vol.43 (6), p.2909-2912</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-5fd237a6d219c3c007cac5480a49f8229f4fa62d03911e5f3052ff6800ccc3de3</citedby><cites>FETCH-LOGICAL-c526t-5fd237a6d219c3c007cac5480a49f8229f4fa62d03911e5f3052ff6800ccc3de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151928/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151928/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,3192,3193,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16856617$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15956416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilson, D. A</creatorcontrib><creatorcontrib>Joyce, M. J</creatorcontrib><creatorcontrib>Hall, L. S</creatorcontrib><creatorcontrib>Reller, L. B</creatorcontrib><creatorcontrib>Roberts, G. D</creatorcontrib><creatorcontrib>Hall, G. S</creatorcontrib><creatorcontrib>Alexander, B. D</creatorcontrib><creatorcontrib>Procop, G. W</creatorcontrib><title>Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively.</description><subject>Bacterial diseases</subject><subject>Bacterial sepsis</subject><subject>Biological and medical sciences</subject><subject>Blood - microbiology</subject><subject>Candida albicans</subject><subject>Candida albicans - classification</subject><subject>Candida albicans - genetics</subject><subject>Candida albicans - isolation & purification</subject><subject>Candidiasis - microbiology</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungemia - microbiology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Nucleic Acid Probes - genetics</subject><subject>Peptide Nucleic Acids - genetics</subject><subject>Predictive Value of Tests</subject><subject>Sensitivity and Specificity</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQhyMEokvhFag5wC2L_8ROfEEqUUuLWqhUKsHJmnXsXVfeeGsnReV5eFAc7cLCiZMP8803HvtXFEcEzwmhzduP7eW8YnMxpxLLkkpC5xRj_qiYESybUgj89XExw1jykhBWHxTPUrrFmFQV50-LA8IlFxURs-Ln5egHp00_mIhO7sGPMLjQo2ARoBb6znWAwC-chj6hK7MZXGfQp1F74zQ61q5Dp34M0aTJgc57dO2GEZ09LKLr3I-t7CqGhUE2RNSuIILOs36X8pxvBlLuTMHDYBKyMazRex9Ch9p8tzGrnxdPLPhkXuzOw-Lm9ORLe1ZefP5w3h5flJpTMZTcdpTVIDpKpGYa41qD5lWDoZK2oVTayoKgHWaSEMMtw5xaKxqMtdasM-yweLf1bsbF2nTTRhG82kS3hvigAjj1b6V3K7UM94oQTiRtsuDNThDD3WjSoNYuP4z30JswJiVqyVhN8X9BUnNBsSAZrLegjiGlaOyf2xCspiioHAVVMSXUFAU1RUFNUcidL_9eZt-3-_sMvN4BkDR4G6HXLu050XAhSJ25V1tu5Zar7y4aBWmtbvV6PzYzR1vGQlCwjNlzc00xYZhgTJuasF-4rNT-</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Wilson, D. A</creator><creator>Joyce, M. J</creator><creator>Hall, L. S</creator><creator>Reller, L. B</creator><creator>Roberts, G. D</creator><creator>Hall, G. S</creator><creator>Alexander, B. D</creator><creator>Procop, G. W</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050601</creationdate><title>Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures</title><author>Wilson, D. A ; Joyce, M. J ; Hall, L. S ; Reller, L. B ; Roberts, G. D ; Hall, G. S ; Alexander, B. D ; Procop, G. W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-5fd237a6d219c3c007cac5480a49f8229f4fa62d03911e5f3052ff6800ccc3de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacterial diseases</topic><topic>Bacterial sepsis</topic><topic>Biological and medical sciences</topic><topic>Blood - microbiology</topic><topic>Candida albicans</topic><topic>Candida albicans - classification</topic><topic>Candida albicans - genetics</topic><topic>Candida albicans - isolation & purification</topic><topic>Candidiasis - microbiology</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungemia - microbiology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Nucleic Acid Probes - genetics</topic><topic>Peptide Nucleic Acids - genetics</topic><topic>Predictive Value of Tests</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilson, D. A</creatorcontrib><creatorcontrib>Joyce, M. J</creatorcontrib><creatorcontrib>Hall, L. S</creatorcontrib><creatorcontrib>Reller, L. B</creatorcontrib><creatorcontrib>Roberts, G. D</creatorcontrib><creatorcontrib>Hall, G. S</creatorcontrib><creatorcontrib>Alexander, B. D</creatorcontrib><creatorcontrib>Procop, G. 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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Bacterial diseases Bacterial sepsis Biological and medical sciences Blood - microbiology Candida albicans Candida albicans - classification Candida albicans - genetics Candida albicans - isolation & purification Candidiasis - microbiology Culture Media Fundamental and applied biological sciences. Psychology Fungemia - microbiology Human bacterial diseases Humans In Situ Hybridization, Fluorescence - methods Infectious diseases Medical sciences Microbiology Miscellaneous Mycology Nucleic Acid Probes - genetics Peptide Nucleic Acids - genetics Predictive Value of Tests Sensitivity and Specificity |
| title | Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures |
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