Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures

We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. al...

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Veröffentlicht in:	Journal of Clinical Microbiology 2005-06, Vol.43 (6), p.2909-2912
Hauptverfasser:	Wilson, D. A, Joyce, M. J, Hall, L. S, Reller, L. B, Roberts, G. D, Hall, G. S, Alexander, B. D, Procop, G. W
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial diseases Bacterial sepsis Biological and medical sciences Blood - microbiology Candida albicans Candida albicans - classification Candida albicans - genetics Candida albicans - isolation & purification Candidiasis - microbiology Culture Media Fundamental and applied biological sciences. Psychology Fungemia - microbiology Human bacterial diseases Humans In Situ Hybridization, Fluorescence - methods Infectious diseases Medical sciences Microbiology Miscellaneous Mycology Nucleic Acid Probes - genetics Peptide Nucleic Acids - genetics Predictive Value of Tests Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.43.6.2909-2912.2005