Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor
AdpA is a key regulator of morphological differentiation in STREPTOMYCES: In contrast to Streptomyces griseus, relatively little is known about AdpA protein functions in Streptomyces coelicolor. Here, we report for the first time the translation accumulation profile of the S. coelicolor adpA (adpASc...
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| Veröffentlicht in: | Journal of Bacteriology 2011-11, Vol.193 (22), p.6358-6365 |
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| creator | Wolański, Marcin Donczew, Rafał Kois-Ostrowska, Agnieszka Masiewicz, Paweł Jakimowicz, Dagmara Zakrzewska-Czerwińska, Jolanta |
| description | AdpA is a key regulator of morphological differentiation in STREPTOMYCES: In contrast to Streptomyces griseus, relatively little is known about AdpA protein functions in Streptomyces coelicolor. Here, we report for the first time the translation accumulation profile of the S. coelicolor adpA (adpASc) gene; the level of S. coelicolor AdpA (AdpASc) increased, reaching a maximum in the early stage of aerial mycelium formation (after 36 h), and remained relatively stable for the next several hours (48 to 60 h), and then the signal intensity decreased considerably. AdpASc specifically binds the adpASc promoter region in vitro and in vivo, suggesting that its expression is autoregulated; surprisingly, in contrast to S. griseus, the protein presumably acts as a transcriptional activator. We also demonstrate a direct influence of AdpASc on the expression of several genes whose products play key roles in the differentiation of S. coelicolor: STI, a protease inhibitor; RamR, an atypical response regulator that itself activates expression of the genes for a small modified peptide that is required for aerial growth; and ClpP1, an ATP-dependent protease. The diverse influence of AdpASc protein on the expression of the analyzed genes presumably results mainly from different affinities of AdpASc protein to individual promoters. |
| doi_str_mv | 10.1128/JB.05734-11 |
| format | Article |
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Here, we report for the first time the translation accumulation profile of the S. coelicolor adpA (adpASc) gene; the level of S. coelicolor AdpA (AdpASc) increased, reaching a maximum in the early stage of aerial mycelium formation (after 36 h), and remained relatively stable for the next several hours (48 to 60 h), and then the signal intensity decreased considerably. AdpASc specifically binds the adpASc promoter region in vitro and in vivo, suggesting that its expression is autoregulated; surprisingly, in contrast to S. griseus, the protein presumably acts as a transcriptional activator. We also demonstrate a direct influence of AdpASc on the expression of several genes whose products play key roles in the differentiation of S. coelicolor: STI, a protease inhibitor; RamR, an atypical response regulator that itself activates expression of the genes for a small modified peptide that is required for aerial growth; and ClpP1, an ATP-dependent protease. The diverse influence of AdpASc protein on the expression of the analyzed genes presumably results mainly from different affinities of AdpASc protein to individual promoters.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/JB.05734-11</identifier><identifier>PMID: 21926228</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Bacterial ; Gene Expression Regulation, Developmental ; genes ; Genetics and Molecular Biology ; Gram-positive bacteria ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Morphology ; mycelium ; Peptides ; promoter regions ; Promoter Regions, Genetic ; protein synthesis ; proteinase inhibitors ; proteinases ; Proteins ; Streptomyces coelicolor ; Streptomyces coelicolor - genetics ; Streptomyces coelicolor - growth & development ; Streptomyces coelicolor - metabolism ; Streptomyces griseus ; Trans-Activators - genetics ; Trans-Activators - metabolism ; transcription factors ; translation (genetics)</subject><ispartof>Journal of Bacteriology, 2011-11, Vol.193 (22), p.6358-6365</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Nov 2011</rights><rights>Copyright © 2011, American Society for Microbiology. All Rights Reserved. 2011 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-a62693b37f5624d78c0b53570487e05cc7191e79e6a8f702d07737450ba5973a3</citedby><cites>FETCH-LOGICAL-c488t-a62693b37f5624d78c0b53570487e05cc7191e79e6a8f702d07737450ba5973a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209205/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209205/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24723556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21926228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolański, Marcin</creatorcontrib><creatorcontrib>Donczew, Rafał</creatorcontrib><creatorcontrib>Kois-Ostrowska, Agnieszka</creatorcontrib><creatorcontrib>Masiewicz, Paweł</creatorcontrib><creatorcontrib>Jakimowicz, Dagmara</creatorcontrib><creatorcontrib>Zakrzewska-Czerwińska, Jolanta</creatorcontrib><title>Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>AdpA is a key regulator of morphological differentiation in STREPTOMYCES: In contrast to Streptomyces griseus, relatively little is known about AdpA protein functions in Streptomyces coelicolor. Here, we report for the first time the translation accumulation profile of the S. coelicolor adpA (adpASc) gene; the level of S. coelicolor AdpA (AdpASc) increased, reaching a maximum in the early stage of aerial mycelium formation (after 36 h), and remained relatively stable for the next several hours (48 to 60 h), and then the signal intensity decreased considerably. AdpASc specifically binds the adpASc promoter region in vitro and in vivo, suggesting that its expression is autoregulated; surprisingly, in contrast to S. griseus, the protein presumably acts as a transcriptional activator. We also demonstrate a direct influence of AdpASc on the expression of several genes whose products play key roles in the differentiation of S. coelicolor: STI, a protease inhibitor; RamR, an atypical response regulator that itself activates expression of the genes for a small modified peptide that is required for aerial growth; and ClpP1, an ATP-dependent protease. The diverse influence of AdpASc protein on the expression of the analyzed genes presumably results mainly from different affinities of AdpASc protein to individual promoters.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Expression Regulation, Developmental</subject><subject>genes</subject><subject>Genetics and Molecular Biology</subject><subject>Gram-positive bacteria</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Morphology</subject><subject>mycelium</subject><subject>Peptides</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>protein synthesis</subject><subject>proteinase inhibitors</subject><subject>proteinases</subject><subject>Proteins</subject><subject>Streptomyces coelicolor</subject><subject>Streptomyces coelicolor - genetics</subject><subject>Streptomyces coelicolor - growth & development</subject><subject>Streptomyces coelicolor - metabolism</subject><subject>Streptomyces griseus</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>transcription factors</subject><subject>translation (genetics)</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks1v0zAYhy0EYmVw4g4REuKAMl5_xfEFqftgMFXiMHbiYDmu07py4sxOB_3vcWjZgJP9yo8e_azfi9BLDCcYk_rD1ekJcEFZifEjNMMg65JzCo_RDIDgUmJJj9CzlDYAmDFOnqIjgiWpCKln6PvC3llfhLaYL4d5ce6iNaPfFfO2zZdUXPwcok3JhX5izic4DJ3tR-2LS9vbVLi-uB6jHcbQ7UyeTbDemeBDfI6etNon--JwHqObTxffzj6Xi6-XX87mi9Kwuh5LXZFK0oaKlleELUVtoOGUC2C1sMCNEVhiK6StdN0KIEsQggrGodFcCqrpMfq49w7bprNLk9NF7dUQXafjTgXt1L8vvVurVbhTlIAkwLPg3UEQw-3WplF1Lhnrve5t2CYlAVMQXLBMvvmP3IRt7PPvMkQ5xhWQDL3fQyaGlKJt76NgUFNl6upU_a4sT5l-9Xf6e_ZPRxl4ewB0Mtq3UffGpQeOCUI5rx6yrd1q_SMXqXTq1KZReQEUIaqifJK93kOtDkqvYhbdXBPAfFoOKRmnvwCLv7Fi</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Wolański, Marcin</creator><creator>Donczew, Rafał</creator><creator>Kois-Ostrowska, Agnieszka</creator><creator>Masiewicz, Paweł</creator><creator>Jakimowicz, Dagmara</creator><creator>Zakrzewska-Czerwińska, Jolanta</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111101</creationdate><title>Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor</title><author>Wolański, Marcin ; Donczew, Rafał ; Kois-Ostrowska, Agnieszka ; Masiewicz, Paweł ; Jakimowicz, Dagmara ; Zakrzewska-Czerwińska, Jolanta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-a62693b37f5624d78c0b53570487e05cc7191e79e6a8f702d07737450ba5973a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Expression Regulation, Developmental</topic><topic>genes</topic><topic>Genetics and Molecular Biology</topic><topic>Gram-positive bacteria</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Morphology</topic><topic>mycelium</topic><topic>Peptides</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>protein synthesis</topic><topic>proteinase inhibitors</topic><topic>proteinases</topic><topic>Proteins</topic><topic>Streptomyces coelicolor</topic><topic>Streptomyces coelicolor - genetics</topic><topic>Streptomyces coelicolor - growth & development</topic><topic>Streptomyces coelicolor - metabolism</topic><topic>Streptomyces griseus</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>transcription factors</topic><topic>translation (genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolański, Marcin</creatorcontrib><creatorcontrib>Donczew, Rafał</creatorcontrib><creatorcontrib>Kois-Ostrowska, Agnieszka</creatorcontrib><creatorcontrib>Masiewicz, Paweł</creatorcontrib><creatorcontrib>Jakimowicz, Dagmara</creatorcontrib><creatorcontrib>Zakrzewska-Czerwińska, Jolanta</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolański, Marcin</au><au>Donczew, Rafał</au><au>Kois-Ostrowska, Agnieszka</au><au>Masiewicz, Paweł</au><au>Jakimowicz, Dagmara</au><au>Zakrzewska-Czerwińska, Jolanta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>193</volume><issue>22</issue><spage>6358</spage><epage>6365</epage><pages>6358-6365</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>AdpA is a key regulator of morphological differentiation in STREPTOMYCES: In contrast to Streptomyces griseus, relatively little is known about AdpA protein functions in Streptomyces coelicolor. Here, we report for the first time the translation accumulation profile of the S. coelicolor adpA (adpASc) gene; the level of S. coelicolor AdpA (AdpASc) increased, reaching a maximum in the early stage of aerial mycelium formation (after 36 h), and remained relatively stable for the next several hours (48 to 60 h), and then the signal intensity decreased considerably. AdpASc specifically binds the adpASc promoter region in vitro and in vivo, suggesting that its expression is autoregulated; surprisingly, in contrast to S. griseus, the protein presumably acts as a transcriptional activator. We also demonstrate a direct influence of AdpASc on the expression of several genes whose products play key roles in the differentiation of S. coelicolor: STI, a protease inhibitor; RamR, an atypical response regulator that itself activates expression of the genes for a small modified peptide that is required for aerial growth; and ClpP1, an ATP-dependent protease. The diverse influence of AdpASc protein on the expression of the analyzed genes presumably results mainly from different affinities of AdpASc protein to individual promoters.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>21926228</pmid><doi>10.1128/JB.05734-11</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Gene Expression Regulation, Developmental genes Genetics and Molecular Biology Gram-positive bacteria Microbiology Miscellaneous Molecular Sequence Data Morphology mycelium Peptides promoter regions Promoter Regions, Genetic protein synthesis proteinase inhibitors proteinases Proteins Streptomyces coelicolor Streptomyces coelicolor - genetics Streptomyces coelicolor - growth & development Streptomyces coelicolor - metabolism Streptomyces griseus Trans-Activators - genetics Trans-Activators - metabolism transcription factors translation (genetics) |
| title | Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor |
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