Enhancement of Innate and Cell-Mediated Immunity by Antimycobacterial Antibodies
We investigated the ability of human antibodies induced by Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination to protect against mycobacterial infections. Serum samples containing mycobacterium-specific antibodies were obtained from volunteers who had received two intradermal BCG vaccin...
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| creator | de Vallière, S Abate, G Blazevic, A Heuertz, R. M Hoft, D. F |
| description | We investigated the ability of human antibodies induced by Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination to protect against mycobacterial infections. Serum samples containing mycobacterium-specific antibodies were obtained from volunteers who had received two intradermal BCG vaccinations 6 months apart. Significant increases in lipoarabinomannan (LAM)-specific immunoglobulin G (IgG) were detected after both the primary and booster vaccinations. Effects of mycobacterium-specific antibodies on surface binding and internalization of BCG by neutrophils and monocytes/macrophages were studied, using green fluorescent protein (gfp)-expressing BCG. Surface-bound gfp-expressing BCG were distinguished from intracellular BCG by surface labeling with LAM-specific monoclonal antibody. Internalization of BCG by phagocytic cells was shown to be significantly enhanced in postvaccination serum samples. Furthermore, the inhibitory effects of neutrophils and monocytes/macrophages on mycobacterial growth were significantly enhanced by BCG-induced antibodies. The growth-inhibiting effects of postvaccination sera were reversed by preabsorption of IgG with Protein G. Finally, the helper effects of antimycobacterial antibodies for the induction of cell-mediated immune responses were investigated. BCG-induced antibodies significantly enhanced proliferation and gamma interferon production in mycobacterium-specific CD4⁺ and CD8⁺ T cells, as well as the proportion of proliferating and degranulating CD8⁺ T cells. We conclude that mycobacterium-specific antibodies are capable of enhancing both innate and cell-mediated immune responses to mycobacteria. |
| doi_str_mv | 10.1128/IAI.73.10.6711-6720.2005 |
| format | Article |
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M ; Hoft, D. F</creator><creatorcontrib>de Vallière, S ; Abate, G ; Blazevic, A ; Heuertz, R. M ; Hoft, D. F</creatorcontrib><description>We investigated the ability of human antibodies induced by Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination to protect against mycobacterial infections. Serum samples containing mycobacterium-specific antibodies were obtained from volunteers who had received two intradermal BCG vaccinations 6 months apart. Significant increases in lipoarabinomannan (LAM)-specific immunoglobulin G (IgG) were detected after both the primary and booster vaccinations. Effects of mycobacterium-specific antibodies on surface binding and internalization of BCG by neutrophils and monocytes/macrophages were studied, using green fluorescent protein (gfp)-expressing BCG. Surface-bound gfp-expressing BCG were distinguished from intracellular BCG by surface labeling with LAM-specific monoclonal antibody. Internalization of BCG by phagocytic cells was shown to be significantly enhanced in postvaccination serum samples. Furthermore, the inhibitory effects of neutrophils and monocytes/macrophages on mycobacterial growth were significantly enhanced by BCG-induced antibodies. The growth-inhibiting effects of postvaccination sera were reversed by preabsorption of IgG with Protein G. Finally, the helper effects of antimycobacterial antibodies for the induction of cell-mediated immune responses were investigated. BCG-induced antibodies significantly enhanced proliferation and gamma interferon production in mycobacterium-specific CD4⁺ and CD8⁺ T cells, as well as the proportion of proliferating and degranulating CD8⁺ T cells. We conclude that mycobacterium-specific antibodies are capable of enhancing both innate and cell-mediated immune responses to mycobacteria.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.73.10.6711-6720.2005</identifier><identifier>PMID: 16177348</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Antibodies, Bacterial - immunology ; BCG Vaccine - immunology ; Biological and medical sciences ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - immunology ; Fundamental and applied biological sciences. Psychology ; Green Fluorescent Proteins - analysis ; Green Fluorescent Proteins - genetics ; Humans ; Immunity, Cellular ; Immunity, Innate ; Immunization, Passive ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Lipopolysaccharides - immunology ; Macrophages - chemistry ; Macrophages - microbiology ; Microbial Immunity and Vaccines ; Microbiology ; Monocytes - chemistry ; Monocytes - microbiology ; Mycobacterium bovis ; Mycobacterium bovis - genetics ; Mycobacterium bovis - immunology ; Neutrophils - chemistry ; Neutrophils - microbiology ; Phagocytosis - immunology ; Tuberculosis - immunology ; Tuberculosis - prevention & control</subject><ispartof>Infection and Immunity, 2005-10, Vol.73 (10), p.6711-6720</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c651t-7060cc6cb1e4bee671838be29ae050b2d610b807874b0298063b457ec3464d4c3</citedby><cites>FETCH-LOGICAL-c651t-7060cc6cb1e4bee671838be29ae050b2d610b807874b0298063b457ec3464d4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1230956/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1230956/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17131340$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16177348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Vallière, S</creatorcontrib><creatorcontrib>Abate, G</creatorcontrib><creatorcontrib>Blazevic, A</creatorcontrib><creatorcontrib>Heuertz, R. M</creatorcontrib><creatorcontrib>Hoft, D. F</creatorcontrib><title>Enhancement of Innate and Cell-Mediated Immunity by Antimycobacterial Antibodies</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>We investigated the ability of human antibodies induced by Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination to protect against mycobacterial infections. Serum samples containing mycobacterium-specific antibodies were obtained from volunteers who had received two intradermal BCG vaccinations 6 months apart. Significant increases in lipoarabinomannan (LAM)-specific immunoglobulin G (IgG) were detected after both the primary and booster vaccinations. Effects of mycobacterium-specific antibodies on surface binding and internalization of BCG by neutrophils and monocytes/macrophages were studied, using green fluorescent protein (gfp)-expressing BCG. Surface-bound gfp-expressing BCG were distinguished from intracellular BCG by surface labeling with LAM-specific monoclonal antibody. Internalization of BCG by phagocytic cells was shown to be significantly enhanced in postvaccination serum samples. Furthermore, the inhibitory effects of neutrophils and monocytes/macrophages on mycobacterial growth were significantly enhanced by BCG-induced antibodies. The growth-inhibiting effects of postvaccination sera were reversed by preabsorption of IgG with Protein G. Finally, the helper effects of antimycobacterial antibodies for the induction of cell-mediated immune responses were investigated. BCG-induced antibodies significantly enhanced proliferation and gamma interferon production in mycobacterium-specific CD4⁺ and CD8⁺ T cells, as well as the proportion of proliferating and degranulating CD8⁺ T cells. We conclude that mycobacterium-specific antibodies are capable of enhancing both innate and cell-mediated immune responses to mycobacteria.</description><subject>Antibodies, Bacterial - immunology</subject><subject>BCG Vaccine - immunology</subject><subject>Biological and medical sciences</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Green Fluorescent Proteins - analysis</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Immunity, Innate</subject><subject>Immunization, Passive</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Lipopolysaccharides - immunology</subject><subject>Macrophages - chemistry</subject><subject>Macrophages - microbiology</subject><subject>Microbial Immunity and Vaccines</subject><subject>Microbiology</subject><subject>Monocytes - chemistry</subject><subject>Monocytes - microbiology</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - genetics</subject><subject>Mycobacterium bovis - immunology</subject><subject>Neutrophils - chemistry</subject><subject>Neutrophils - microbiology</subject><subject>Phagocytosis - immunology</subject><subject>Tuberculosis - immunology</subject><subject>Tuberculosis - prevention & control</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP0zAQhS0EYsvCX4AICW4pM7FjOxekqlog0iKQYM-W7TitUeIsdgrqv8ehFYUTJ2vsb57nzSOkQFgjVvJNu2nXgq5zyQViyUUF6wqgfkBWCI0s67qqHpIVADZlU3NxRZ6k9C2XjDH5mFwhRyEokyvy-SbsdbBudGEupr5oQ9CzK3Toiq0bhvKj63y-6Ip2HA_Bz8fCHItNmP14tJPRdnbR6-H3jZk679JT8qjXQ3LPzuc1uXt383X7obz99L7dbm5Ly2ucSwEcrOXWoGPGuWxDUmlc1WgHNZiq4whGgpCCGagaCZwaVgtnKeOsY5Zek7cn3fuDGV1n8_xRD-o--lHHo5q0V_--BL9Xu-mHwopC3kkWeH0WiNP3g0uzGn2y2bMObjokxSVHCgL-C6JgyIAtivIE2jilFF3_ZxoEteSmcm5K0KVcclNLbmrJLbc-_9vNpfEcVAZenQGdrB76mEPz6cIJpEjZMuzLE7f3u_1PH53SaVQ-b-Pyb4ZenKBeT0rvYha6-1JBtot518gF_QVs-bWP</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>de Vallière, S</creator><creator>Abate, G</creator><creator>Blazevic, A</creator><creator>Heuertz, R. M</creator><creator>Hoft, D. F</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20051001</creationdate><title>Enhancement of Innate and Cell-Mediated Immunity by Antimycobacterial Antibodies</title><author>de Vallière, S ; Abate, G ; Blazevic, A ; Heuertz, R. M ; Hoft, D. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c651t-7060cc6cb1e4bee671838be29ae050b2d610b807874b0298063b457ec3464d4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antibodies, Bacterial - immunology</topic><topic>BCG Vaccine - immunology</topic><topic>Biological and medical sciences</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Green Fluorescent Proteins - analysis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Immunity, Innate</topic><topic>Immunization, Passive</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - immunology</topic><topic>Lipopolysaccharides - immunology</topic><topic>Macrophages - chemistry</topic><topic>Macrophages - microbiology</topic><topic>Microbial Immunity and Vaccines</topic><topic>Microbiology</topic><topic>Monocytes - chemistry</topic><topic>Monocytes - microbiology</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - genetics</topic><topic>Mycobacterium bovis - immunology</topic><topic>Neutrophils - chemistry</topic><topic>Neutrophils - microbiology</topic><topic>Phagocytosis - immunology</topic><topic>Tuberculosis - immunology</topic><topic>Tuberculosis - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Vallière, S</creatorcontrib><creatorcontrib>Abate, G</creatorcontrib><creatorcontrib>Blazevic, A</creatorcontrib><creatorcontrib>Heuertz, R. 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M</au><au>Hoft, D. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of Innate and Cell-Mediated Immunity by Antimycobacterial Antibodies</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>73</volume><issue>10</issue><spage>6711</spage><epage>6720</epage><pages>6711-6720</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>We investigated the ability of human antibodies induced by Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination to protect against mycobacterial infections. Serum samples containing mycobacterium-specific antibodies were obtained from volunteers who had received two intradermal BCG vaccinations 6 months apart. Significant increases in lipoarabinomannan (LAM)-specific immunoglobulin G (IgG) were detected after both the primary and booster vaccinations. Effects of mycobacterium-specific antibodies on surface binding and internalization of BCG by neutrophils and monocytes/macrophages were studied, using green fluorescent protein (gfp)-expressing BCG. Surface-bound gfp-expressing BCG were distinguished from intracellular BCG by surface labeling with LAM-specific monoclonal antibody. Internalization of BCG by phagocytic cells was shown to be significantly enhanced in postvaccination serum samples. Furthermore, the inhibitory effects of neutrophils and monocytes/macrophages on mycobacterial growth were significantly enhanced by BCG-induced antibodies. The growth-inhibiting effects of postvaccination sera were reversed by preabsorption of IgG with Protein G. Finally, the helper effects of antimycobacterial antibodies for the induction of cell-mediated immune responses were investigated. BCG-induced antibodies significantly enhanced proliferation and gamma interferon production in mycobacterium-specific CD4⁺ and CD8⁺ T cells, as well as the proportion of proliferating and degranulating CD8⁺ T cells. We conclude that mycobacterium-specific antibodies are capable of enhancing both innate and cell-mediated immune responses to mycobacteria.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16177348</pmid><doi>10.1128/IAI.73.10.6711-6720.2005</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies, Bacterial - immunology BCG Vaccine - immunology Biological and medical sciences CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - immunology Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics Humans Immunity, Cellular Immunity, Innate Immunization, Passive Immunoglobulin G - blood Immunoglobulin G - immunology Lipopolysaccharides - immunology Macrophages - chemistry Macrophages - microbiology Microbial Immunity and Vaccines Microbiology Monocytes - chemistry Monocytes - microbiology Mycobacterium bovis Mycobacterium bovis - genetics Mycobacterium bovis - immunology Neutrophils - chemistry Neutrophils - microbiology Phagocytosis - immunology Tuberculosis - immunology Tuberculosis - prevention & control |
| title | Enhancement of Innate and Cell-Mediated Immunity by Antimycobacterial Antibodies |
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