The immunoprotective Anaplasma marginale major surface protein 2 is encoded by a polymorphic multigene family
An Anaplasma marginale Florida msp-2 gene was cloned and expressed in Escherichia coli. Pulsed-field gel electrophoresis and Southern blot analysis revealed the presence of multiple msp-2 gene copies that were widely distributed throughout the chromosomes of all three strains examined. Genomic polym...
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| Veröffentlicht in: | Infection and Immunity 1994-09, Vol.62 (9), p.3808-3816 |
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| creator | Palmer, G.H. (Washington State University, Pullman, WA.) Eid, G Barbet, A.F McGuire, T.C McElwain, T.F |
| description | An Anaplasma marginale Florida msp-2 gene was cloned and expressed in Escherichia coli. Pulsed-field gel electrophoresis and Southern blot analysis revealed the presence of multiple msp-2 gene copies that were widely distributed throughout the chromosomes of all three strains examined. Genomic polymorphism among copies was greatest in the 5' end of msp-2 but also occurred in 3' regions. The presence of gene-copy-specific epitopes was indicated by the reactivity of the cloned msp-2 copy with some, but not all, monoclonal antibodies that bound native MSP-2. Multiple antigenically distinct MSP-2 molecules were expressed within strains and were coexpressed by individual A. marginale organisms. These results suggest that expression of polymorphic msp-2 gene copies is responsible for the significant percentages of A. marginale organisms within strains that do not react with individual anti-MSP-2 monoclonal antibodies. Sequence analysis revealed highly significant MSP-2 homology with two rickettsial surface proteins, A. marginale MSP-4 and Cowdria ruminantium MAP-1. Immunization with MSP-4 has been shown to induce protective immunity in a manner similar to that of immunization with MSP-2. These findings support the hypothesis that A. marginale surface proteins are targets of protective immune responses but are antigenically polymorphic |
| doi_str_mv | 10.1128/IAI.62.9.3808-3816.1994 |
| format | Article |
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(Washington State University, Pullman, WA.) ; Eid, G ; Barbet, A.F ; McGuire, T.C ; McElwain, T.F</creator><creatorcontrib>Palmer, G.H. (Washington State University, Pullman, WA.) ; Eid, G ; Barbet, A.F ; McGuire, T.C ; McElwain, T.F</creatorcontrib><description>An Anaplasma marginale Florida msp-2 gene was cloned and expressed in Escherichia coli. Pulsed-field gel electrophoresis and Southern blot analysis revealed the presence of multiple msp-2 gene copies that were widely distributed throughout the chromosomes of all three strains examined. Genomic polymorphism among copies was greatest in the 5' end of msp-2 but also occurred in 3' regions. The presence of gene-copy-specific epitopes was indicated by the reactivity of the cloned msp-2 copy with some, but not all, monoclonal antibodies that bound native MSP-2. Multiple antigenically distinct MSP-2 molecules were expressed within strains and were coexpressed by individual A. marginale organisms. These results suggest that expression of polymorphic msp-2 gene copies is responsible for the significant percentages of A. marginale organisms within strains that do not react with individual anti-MSP-2 monoclonal antibodies. Sequence analysis revealed highly significant MSP-2 homology with two rickettsial surface proteins, A. marginale MSP-4 and Cowdria ruminantium MAP-1. Immunization with MSP-4 has been shown to induce protective immunity in a manner similar to that of immunization with MSP-2. These findings support the hypothesis that A. marginale surface proteins are targets of protective immune responses but are antigenically polymorphic</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.62.9.3808-3816.1994</identifier><identifier>PMID: 8063397</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Amino Acid Sequence ; ANAPLASMA ; Anaplasma - genetics ; Anaplasma - immunology ; Anaplasma marginale ; Antibodies, Monoclonal - immunology ; ANTIGENE ; ANTIGENOS ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; CLONACION ; CLONAGE ; Cloning, Molecular ; DIFERENCIAS BIOLOGICAS ; DIFFERENCE BIOLOGIQUE ; ESCHERICHIA COLI ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Bacterial ; Genetics ; GLICOPROTEINAS ; GLYCOPROTEINE ; Microbiology ; Molecular Sequence Data ; Multigene Family ; POLIMORFISMO GENETICO ; POLYMORPHISME GENETIQUE ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE</subject><ispartof>Infection and Immunity, 1994-09, Vol.62 (9), p.3808-3816</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-dfcd5d973dd5ba536226fffefaf59cc3b35f7e4ba3d8934d17381ad87cc44243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC303035/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC303035/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4261563$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8063397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palmer, G.H. (Washington State University, Pullman, WA.)</creatorcontrib><creatorcontrib>Eid, G</creatorcontrib><creatorcontrib>Barbet, A.F</creatorcontrib><creatorcontrib>McGuire, T.C</creatorcontrib><creatorcontrib>McElwain, T.F</creatorcontrib><title>The immunoprotective Anaplasma marginale major surface protein 2 is encoded by a polymorphic multigene family</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>An Anaplasma marginale Florida msp-2 gene was cloned and expressed in Escherichia coli. Pulsed-field gel electrophoresis and Southern blot analysis revealed the presence of multiple msp-2 gene copies that were widely distributed throughout the chromosomes of all three strains examined. Genomic polymorphism among copies was greatest in the 5' end of msp-2 but also occurred in 3' regions. The presence of gene-copy-specific epitopes was indicated by the reactivity of the cloned msp-2 copy with some, but not all, monoclonal antibodies that bound native MSP-2. Multiple antigenically distinct MSP-2 molecules were expressed within strains and were coexpressed by individual A. marginale organisms. These results suggest that expression of polymorphic msp-2 gene copies is responsible for the significant percentages of A. marginale organisms within strains that do not react with individual anti-MSP-2 monoclonal antibodies. Sequence analysis revealed highly significant MSP-2 homology with two rickettsial surface proteins, A. marginale MSP-4 and Cowdria ruminantium MAP-1. Immunization with MSP-4 has been shown to induce protective immunity in a manner similar to that of immunization with MSP-2. These findings support the hypothesis that A. marginale surface proteins are targets of protective immune responses but are antigenically polymorphic</description><subject>Amino Acid Sequence</subject><subject>ANAPLASMA</subject><subject>Anaplasma - genetics</subject><subject>Anaplasma - immunology</subject><subject>Anaplasma marginale</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>Cloning, Molecular</subject><subject>DIFERENCIAS BIOLOGICAS</subject><subject>DIFFERENCE BIOLOGIQUE</subject><subject>ESCHERICHIA COLI</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>POLIMORFISMO GENETICO</subject><subject>POLYMORPHISME GENETIQUE</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUurEzEYhoMox1r9A4IYQdxNnVxnsnBRDl4KB1xY1-FrLm0OM5MxmTnSf29qS_GsJIskfM_73V6E3pJ6RQhtP27Wm5WkK7Vibd1WrCVyRZTiT9CC1KqthKD0KVrUNVGVErJ5jl7kfF--nPP2Bt20tWRMNQvUbw8Oh76fhzimODkzhQeH1wOMHeQecA9pHwboXHndx4TznDwYh__CYcAUh4zdYKJ1Fu-OGPAYu2Mf03gIBvdzN4W9Gxz20Ifu-BI989Bl9-pyL9H2y-ft7bfq7vvXze36rjKCyKmy3lhhVcOsFTsQTFIqvffOgxfKGLZjwjeO74DZVjFuSVMWALZtjOGccrZEn85px3nXO2vcMCXo9JhCGeeoIwT9ODKEg97HB83qckTRf7joU_w1uzzpPmTjug4GF-esGyklJ_L_IJGqKdunBWzOoEkx5-T8tRlS65OhOpSmJNVKnwzVJ0P1ydCifPPvLFfdxcESf3-JQzbQ-QSDCfmKcSqJKOQSvTtjh7A__A7J6WLv46KFeX1mPEQN-1TS_PyhBGdtw9kfBy_A5g</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Palmer, G.H. (Washington State University, Pullman, WA.)</creator><creator>Eid, G</creator><creator>Barbet, A.F</creator><creator>McGuire, T.C</creator><creator>McElwain, T.F</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19940901</creationdate><title>The immunoprotective Anaplasma marginale major surface protein 2 is encoded by a polymorphic multigene family</title><author>Palmer, G.H. (Washington State University, Pullman, WA.) ; Eid, G ; Barbet, A.F ; McGuire, T.C ; McElwain, T.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-dfcd5d973dd5ba536226fffefaf59cc3b35f7e4ba3d8934d17381ad87cc44243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>ANAPLASMA</topic><topic>Anaplasma - genetics</topic><topic>Anaplasma - immunology</topic><topic>Anaplasma marginale</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>Cloning, Molecular</topic><topic>DIFERENCIAS BIOLOGICAS</topic><topic>DIFFERENCE BIOLOGIQUE</topic><topic>ESCHERICHIA COLI</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>POLIMORFISMO GENETICO</topic><topic>POLYMORPHISME GENETIQUE</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palmer, G.H. (Washington State University, Pullman, WA.)</creatorcontrib><creatorcontrib>Eid, G</creatorcontrib><creatorcontrib>Barbet, A.F</creatorcontrib><creatorcontrib>McGuire, T.C</creatorcontrib><creatorcontrib>McElwain, T.F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palmer, G.H. (Washington State University, Pullman, WA.)</au><au>Eid, G</au><au>Barbet, A.F</au><au>McGuire, T.C</au><au>McElwain, T.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immunoprotective Anaplasma marginale major surface protein 2 is encoded by a polymorphic multigene family</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>62</volume><issue>9</issue><spage>3808</spage><epage>3816</epage><pages>3808-3816</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>An Anaplasma marginale Florida msp-2 gene was cloned and expressed in Escherichia coli. Pulsed-field gel electrophoresis and Southern blot analysis revealed the presence of multiple msp-2 gene copies that were widely distributed throughout the chromosomes of all three strains examined. Genomic polymorphism among copies was greatest in the 5' end of msp-2 but also occurred in 3' regions. The presence of gene-copy-specific epitopes was indicated by the reactivity of the cloned msp-2 copy with some, but not all, monoclonal antibodies that bound native MSP-2. Multiple antigenically distinct MSP-2 molecules were expressed within strains and were coexpressed by individual A. marginale organisms. These results suggest that expression of polymorphic msp-2 gene copies is responsible for the significant percentages of A. marginale organisms within strains that do not react with individual anti-MSP-2 monoclonal antibodies. Sequence analysis revealed highly significant MSP-2 homology with two rickettsial surface proteins, A. marginale MSP-4 and Cowdria ruminantium MAP-1. Immunization with MSP-4 has been shown to induce protective immunity in a manner similar to that of immunization with MSP-2. These findings support the hypothesis that A. marginale surface proteins are targets of protective immune responses but are antigenically polymorphic</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8063397</pmid><doi>10.1128/IAI.62.9.3808-3816.1994</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Amino Acid Sequence ANAPLASMA Anaplasma - genetics Anaplasma - immunology Anaplasma marginale Antibodies, Monoclonal - immunology ANTIGENE ANTIGENOS Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - immunology Bacteriology Base Sequence Biological and medical sciences CLONACION CLONAGE Cloning, Molecular DIFERENCIAS BIOLOGICAS DIFFERENCE BIOLOGIQUE ESCHERICHIA COLI EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENES Genes, Bacterial Genetics GLICOPROTEINAS GLYCOPROTEINE Microbiology Molecular Sequence Data Multigene Family POLIMORFISMO GENETICO POLYMORPHISME GENETIQUE SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE |
| title | The immunoprotective Anaplasma marginale major surface protein 2 is encoded by a polymorphic multigene family |
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