Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol
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| Veröffentlicht in: | Applied and Environmental Microbiology 2014-02, Vol.80 (4), p.1515-1527 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Dror, Adi Shemesh, Einav Dayan, Natali Fishman, Ayelet |
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| doi_str_mv | 10.1128/AEM.03371-13 |
| format | Article |
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</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.03371-13</identifier><identifier>PMID: 24362426</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Amino Acid Substitution ; Enzyme Inhibitors - metabolism ; Enzyme Stability ; Enzymes ; Enzymology and Protein Engineering ; Geobacillus ; Geobacillus stearothermophilus - enzymology ; Geobacillus stearothermophilus - genetics ; Gram-positive bacteria ; Hydrogen bonds ; Lipase - chemistry ; Lipase - genetics ; Lipase - metabolism ; Methanol ; Methanol - metabolism ; Models, Molecular ; Molecular Dynamics Simulation ; Mutagenesis ; Mutant Proteins - chemistry ; Mutant Proteins - genetics ; Mutant Proteins - metabolism ; Protein Engineering - methods ; Proteins ; Solvents</subject><ispartof>Applied and Environmental Microbiology, 2014-02, Vol.80 (4), p.1515-1527</ispartof><rights>Copyright American Society for Microbiology Feb 2014</rights><rights>Copyright © 2014, American Society for Microbiology. All Rights Reserved. 2014 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-ac8f573e9c474764287f4c9a9ac9c8df834714a9f9671b016eea4fee748b0ecf3</citedby><cites>FETCH-LOGICAL-c514t-ac8f573e9c474764287f4c9a9ac9c8df834714a9f9671b016eea4fee748b0ecf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911059/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911059/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24362426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dror, Adi</creatorcontrib><creatorcontrib>Shemesh, Einav</creatorcontrib><creatorcontrib>Dayan, Natali</creatorcontrib><creatorcontrib>Fishman, Ayelet</creatorcontrib><title>Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Classifications
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</description><subject>Amino Acid Substitution</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Enzymology and Protein Engineering</subject><subject>Geobacillus</subject><subject>Geobacillus stearothermophilus - enzymology</subject><subject>Geobacillus stearothermophilus - genetics</subject><subject>Gram-positive bacteria</subject><subject>Hydrogen bonds</subject><subject>Lipase - chemistry</subject><subject>Lipase - genetics</subject><subject>Lipase - metabolism</subject><subject>Methanol</subject><subject>Methanol - metabolism</subject><subject>Models, Molecular</subject><subject>Molecular Dynamics Simulation</subject><subject>Mutagenesis</subject><subject>Mutant Proteins - chemistry</subject><subject>Mutant Proteins - genetics</subject><subject>Mutant Proteins - metabolism</subject><subject>Protein Engineering - methods</subject><subject>Proteins</subject><subject>Solvents</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUFv1DAQhS0EotvCjTMy4tIDKXbsOPEFqVotC9KuQFDOluOMN64Se2snoP0l_F28bKmA08jPn9680UPoBSVXlJbN2-vV9oowVtOCskdoQYlsioox8RgtCJGyKEtOztB5SreEEE5E8xSdlZyJkpdigX5-jmEC5_HK75wHiM7vcHvAX7Tvwoi386R34CG5hLOCv05xNtMcoVjProMOL4NP4NOccLB4DaHVxg1DfqYJdLbuIY5h37ujtHF7nQDfCGxDzAt77Q0cPXXrBjcdcI6xhSnLYXiGnlg9JHh-Py_Qt_erm-WHYvNp_XF5vSlMRflUaNPYqmYgDa95LXjZ1JYbqaU20jSdbRivKdfSSlHTllABoLkFqHnTEjCWXaB3J9_93I7QGfBT1IPaRzfqeFBBO_Xvj3e92oXviklKSSWzweW9QQx3M6RJjS4ZGAbtIcxJ0YrkNlhd0oy-_g-9DXP0-TxFuRSCN7IimXpzokwMKUWwD2EoUcfGVW5c_W5cUZbxl38f8AD_qTgDr05A73b9DxdB6TQqDaNqiOI5H63YL3GytSc</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Dror, Adi</creator><creator>Shemesh, Einav</creator><creator>Dayan, Natali</creator><creator>Fishman, Ayelet</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20140201</creationdate><title>Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol</title><author>Dror, Adi ; 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</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>24362426</pmid><doi>10.1128/AEM.03371-13</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2014-02, Vol.80 (4), p.1515-1527 |
| issn | 0099-2240 1098-5336 1098-6596 |
| language | eng |
| recordid | cdi_highwire_asm_aem_80_4_1515 |
| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Amino Acid Substitution Enzyme Inhibitors - metabolism Enzyme Stability Enzymes Enzymology and Protein Engineering Geobacillus Geobacillus stearothermophilus - enzymology Geobacillus stearothermophilus - genetics Gram-positive bacteria Hydrogen bonds Lipase - chemistry Lipase - genetics Lipase - metabolism Methanol Methanol - metabolism Models, Molecular Molecular Dynamics Simulation Mutagenesis Mutant Proteins - chemistry Mutant Proteins - genetics Mutant Proteins - metabolism Protein Engineering - methods Proteins Solvents |
| title | Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol |
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