Biodegradation of a Biocide (Cu-N-Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community
The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HD...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2010-12, Vol.76 (24), p.8076-8083 |
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| creator | Jakobs-Schönwandt, Désirée Mathies, Helena Abraham, Wolf-Rainer Pritzkow, Wolfgang Stephan, Ina Noll, Matthias |
| description | The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled (¹³C) and nonlabeled (¹²C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the δ¹³C content of respired CO₂, as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the ¹³CO₂ concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C₁₉:₀d8,9, C₁₈:₀, C₁₈:₁ω7, C₁₈:₂ω6,9, C₁₇:₁d7,8, C₁₆:₀, and C₁₆:₁ω7 were highly enriched in their δ¹³C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely. |
| doi_str_mv | 10.1128/AEM.01092-10 |
| format | Article |
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One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled (¹³C) and nonlabeled (¹²C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the δ¹³C content of respired CO₂, as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the ¹³CO₂ concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C₁₉:₀d8,9, C₁₈:₀, C₁₈:₁ω7, C₁₈:₂ω6,9, C₁₇:₁d7,8, C₁₆:₀, and C₁₆:₁ω7 were highly enriched in their δ¹³C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.01092-10</identifier><identifier>PMID: 20952650</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - metabolism ; Bacteriology ; Biodegradation ; Biological and medical sciences ; Biopesticides ; Biotransformation ; Carbon Isotopes - metabolism ; Chelation therapy ; Chromatography ; Chromatography, High Pressure Liquid ; Cluster Analysis ; Comamonas ; Copper - metabolism ; Cyclohexanes - metabolism ; Disinfectants - metabolism ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Imides - metabolism ; Isotopes ; Mass spectrometry ; Microbiology ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Soil Microbiology ; Staining and Labeling - methods ; Studies ; Wood preservatives</subject><ispartof>Applied and Environmental Microbiology, 2010-12, Vol.76 (24), p.8076-8083</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Dec 2010</rights><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-1cf51dae25cb0ebb4585c9bd7b5398bc19491821a27d15396f22137819f0c6843</citedby><cites>FETCH-LOGICAL-c598t-1cf51dae25cb0ebb4585c9bd7b5398bc19491821a27d15396f22137819f0c6843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008219/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008219/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23635094$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20952650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jakobs-Schönwandt, Désirée</creatorcontrib><creatorcontrib>Mathies, Helena</creatorcontrib><creatorcontrib>Abraham, Wolf-Rainer</creatorcontrib><creatorcontrib>Pritzkow, Wolfgang</creatorcontrib><creatorcontrib>Stephan, Ina</creatorcontrib><creatorcontrib>Noll, Matthias</creatorcontrib><title>Biodegradation of a Biocide (Cu-N-Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled (¹³C) and nonlabeled (¹²C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the δ¹³C content of respired CO₂, as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the ¹³CO₂ concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C₁₉:₀d8,9, C₁₈:₀, C₁₈:₁ω7, C₁₈:₂ω6,9, C₁₇:₁d7,8, C₁₆:₀, and C₁₆:₁ω7 were highly enriched in their δ¹³C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely.</description><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Bacteriology</subject><subject>Biodegradation</subject><subject>Biological and medical sciences</subject><subject>Biopesticides</subject><subject>Biotransformation</subject><subject>Carbon Isotopes - metabolism</subject><subject>Chelation therapy</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cluster Analysis</subject><subject>Comamonas</subject><subject>Copper - metabolism</subject><subject>Cyclohexanes - metabolism</subject><subject>Disinfectants - metabolism</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imides - metabolism</subject><subject>Isotopes</subject><subject>Mass spectrometry</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Soil Microbiology</subject><subject>Staining and Labeling - methods</subject><subject>Studies</subject><subject>Wood preservatives</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1v1DAQxSMEotvCjTNESKggkTJ2Yse-ILVp-ZDKh1QqjpbjTHZdJfFiJ0uXO_83bndpgQsnW8-_eZrxmyR5ROCAECpeHZ58OAACkmYE7iSzeBMZy3N-N5kBSJlRWsBOshvCBQAUwMX9ZIeCZJQzmCU_j6xrcO51o0frhtS1qU6jZmyD6fNqyj5m1dp0boGX666x-gcOdurTY-suI_EirVy_dAMO46byq3NN-tljQL-KhitM63WUj7G1AzbpmbNdeqTNiN7q7qq4nwY7rh8k91rdBXy4PfeS8zcnX6p32emnt--rw9PMMCnGjJiWkUYjZaYGrOuCCWZk3ZQ1y6WoDZGFJIISTcuGRIm3lJK8FES2YLgo8r3k9cZ3OdU9Nib27XWnlt722q-V01b9_TLYhZq7lcoBoq-MBvtbA---TRhG1dtgsOv0gG4KSrKCxyig_C8pGC85F4JF8uk_5IWb_BD_QQkak6LsGnq5gYx3IXhsb5omoK72QMU9UNd7EJWIP_5z0Bv4d_AReLYFdDC6a70ejA23XM5zBrK4bW5h54vv1qPSoVcae1VyRQsloOQRerKBWu2UnvtodH5GgeRAJCljAvkviE_O-w</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Jakobs-Schönwandt, Désirée</creator><creator>Mathies, Helena</creator><creator>Abraham, Wolf-Rainer</creator><creator>Pritzkow, Wolfgang</creator><creator>Stephan, Ina</creator><creator>Noll, Matthias</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7QH</scope><scope>7UA</scope><scope>5PM</scope></search><sort><creationdate>20101201</creationdate><title>Biodegradation of a Biocide (Cu-N-Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community</title><author>Jakobs-Schönwandt, Désirée ; Mathies, Helena ; Abraham, Wolf-Rainer ; Pritzkow, Wolfgang ; Stephan, Ina ; Noll, Matthias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-1cf51dae25cb0ebb4585c9bd7b5398bc19491821a27d15396f22137819f0c6843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Bacteriology</topic><topic>Biodegradation</topic><topic>Biological and medical sciences</topic><topic>Biopesticides</topic><topic>Biotransformation</topic><topic>Carbon Isotopes - metabolism</topic><topic>Chelation therapy</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cluster Analysis</topic><topic>Comamonas</topic><topic>Copper - metabolism</topic><topic>Cyclohexanes - metabolism</topic><topic>Disinfectants - metabolism</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imides - metabolism</topic><topic>Isotopes</topic><topic>Mass spectrometry</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Soil Microbiology</topic><topic>Staining and Labeling - methods</topic><topic>Studies</topic><topic>Wood preservatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jakobs-Schönwandt, Désirée</creatorcontrib><creatorcontrib>Mathies, Helena</creatorcontrib><creatorcontrib>Abraham, Wolf-Rainer</creatorcontrib><creatorcontrib>Pritzkow, Wolfgang</creatorcontrib><creatorcontrib>Stephan, Ina</creatorcontrib><creatorcontrib>Noll, Matthias</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jakobs-Schönwandt, Désirée</au><au>Mathies, Helena</au><au>Abraham, Wolf-Rainer</au><au>Pritzkow, Wolfgang</au><au>Stephan, Ina</au><au>Noll, Matthias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biodegradation of a Biocide (Cu-N-Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>76</volume><issue>24</issue><spage>8076</spage><epage>8083</epage><pages>8076-8083</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled (¹³C) and nonlabeled (¹²C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the δ¹³C content of respired CO₂, as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the ¹³CO₂ concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C₁₉:₀d8,9, C₁₈:₀, C₁₈:₁ω7, C₁₈:₂ω6,9, C₁₇:₁d7,8, C₁₆:₀, and C₁₆:₁ω7 were highly enriched in their δ¹³C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20952650</pmid><doi>10.1128/AEM.01092-10</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 2010-12, Vol.76 (24), p.8076-8083 |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteria Bacteria - classification Bacteria - genetics Bacteria - metabolism Bacteriology Biodegradation Biological and medical sciences Biopesticides Biotransformation Carbon Isotopes - metabolism Chelation therapy Chromatography Chromatography, High Pressure Liquid Cluster Analysis Comamonas Copper - metabolism Cyclohexanes - metabolism Disinfectants - metabolism DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Imides - metabolism Isotopes Mass spectrometry Microbiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Soil Microbiology Staining and Labeling - methods Studies Wood preservatives |
| title | Biodegradation of a Biocide (Cu-N-Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community |
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