Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates
A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two Pantoea spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2010-10, Vol.76 (19), p.6463-6469 |
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| creator | Shan, Huifeng Kurtz, Harry D. Jr Mykytczuk, Nadia Trevors, Jack T Freedman, David L |
| description | A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two Pantoea spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to increasing levels of CF, corn syrup-grown DHM-1 was able to transform over 500 mg/liter CF in the presence of vitamin B₁₂ (approximately 3% of CF on a molar basis) at a rate as high as 22 mg/liter/day in a mineral salts medium. CO, CO₂, and organic acids were the predominant biodegradation products, suggesting that hydrolytic reactions predominate during CF transformation. DHM-1 was capable of growing on corn syrup in the presence of high concentrations of CF (as may be present near contaminant source zones in groundwater), which makes it a promising culture for bioaugmentation. Strains DHM-1B and DHM-1T transform CF at rates similar to that of the DHM-1 enrichment culture. The ability of these strains to grow in the presence of high concentrations of CF appears to be related to alteration of membrane fluidity or homeoviscous and homeophasic adaptation. |
| doi_str_mv | 10.1128/aem.01191-10 |
| format | Article |
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Jr ; Mykytczuk, Nadia ; Trevors, Jack T ; Freedman, David L</creator><creatorcontrib>Shan, Huifeng ; Kurtz, Harry D. Jr ; Mykytczuk, Nadia ; Trevors, Jack T ; Freedman, David L</creatorcontrib><description>A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two Pantoea spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to increasing levels of CF, corn syrup-grown DHM-1 was able to transform over 500 mg/liter CF in the presence of vitamin B₁₂ (approximately 3% of CF on a molar basis) at a rate as high as 22 mg/liter/day in a mineral salts medium. CO, CO₂, and organic acids were the predominant biodegradation products, suggesting that hydrolytic reactions predominate during CF transformation. DHM-1 was capable of growing on corn syrup in the presence of high concentrations of CF (as may be present near contaminant source zones in groundwater), which makes it a promising culture for bioaugmentation. Strains DHM-1B and DHM-1T transform CF at rates similar to that of the DHM-1 enrichment culture. The ability of these strains to grow in the presence of high concentrations of CF appears to be related to alteration of membrane fluidity or homeoviscous and homeophasic adaptation.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/aem.01191-10</identifier><identifier>PMID: 20693443</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Acclimation ; Anaerobiosis ; Bacteria ; Bioconversions. Hemisynthesis ; Biodegradation ; Biological and medical sciences ; Biotechnology ; Biotransformation ; Carbon Dioxide - metabolism ; Carbon Monoxide - metabolism ; Carboxylic Acids - metabolism ; Chlorine - metabolism ; Chloroform - metabolism ; Culture Media - chemistry ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fatty Acids - analysis ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Metabolism ; Methods. Procedures. Technologies ; Microbiology ; Molecular Sequence Data ; Pantoea - classification ; Pantoea - growth & development ; Pantoea - isolation & purification ; Pantoea - metabolism ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Soil Microbiology ; Vitamin B ; Vitamin B 12 - metabolism</subject><ispartof>Applied and Environmental Microbiology, 2010-10, Vol.76 (19), p.6463-6469</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Oct 2010</rights><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c621t-16e7e65afec8413a585f5fc8fc62e964e17c8920f266304dd461ea5467d27b653</citedby><cites>FETCH-LOGICAL-c621t-16e7e65afec8413a585f5fc8fc62e964e17c8920f266304dd461ea5467d27b653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950468/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950468/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23285809$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20693443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shan, Huifeng</creatorcontrib><creatorcontrib>Kurtz, Harry D. Jr</creatorcontrib><creatorcontrib>Mykytczuk, Nadia</creatorcontrib><creatorcontrib>Trevors, Jack T</creatorcontrib><creatorcontrib>Freedman, David L</creatorcontrib><title>Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two Pantoea spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to increasing levels of CF, corn syrup-grown DHM-1 was able to transform over 500 mg/liter CF in the presence of vitamin B₁₂ (approximately 3% of CF on a molar basis) at a rate as high as 22 mg/liter/day in a mineral salts medium. CO, CO₂, and organic acids were the predominant biodegradation products, suggesting that hydrolytic reactions predominate during CF transformation. DHM-1 was capable of growing on corn syrup in the presence of high concentrations of CF (as may be present near contaminant source zones in groundwater), which makes it a promising culture for bioaugmentation. Strains DHM-1B and DHM-1T transform CF at rates similar to that of the DHM-1 enrichment culture. The ability of these strains to grow in the presence of high concentrations of CF appears to be related to alteration of membrane fluidity or homeoviscous and homeophasic adaptation.</description><subject>Acclimation</subject><subject>Anaerobiosis</subject><subject>Bacteria</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biodegradation</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotransformation</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carbon Monoxide - metabolism</subject><subject>Carboxylic Acids - metabolism</subject><subject>Chlorine - metabolism</subject><subject>Chloroform - metabolism</subject><subject>Culture Media - chemistry</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fatty Acids - analysis</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Pantoea - classification</subject><subject>Pantoea - growth & development</subject><subject>Pantoea - isolation & purification</subject><subject>Pantoea - metabolism</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Soil Microbiology</subject><subject>Vitamin B</subject><subject>Vitamin B 12 - metabolism</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1v1DAQxSMEokvhxhksJMSFlPFnnAvSNlpopSIOtGfL69gbV0lc7ISq_z0Ou7TAiZOl9356mpnnoniJ4QRjIj9oO5wAxjUuMTwqVhhqWXJKxeNiBVDXJSEMjopnKV0DAAMhnxZHBERNGaOrYlqP2saw9Qad-jBFPSYX4qAnH0YUHDrzuw41YTR2zOaipkVuuj7EsJBoe4f0iDZj9KYbMoWauZ_maLPaosvbgE61mWz0ukfnKfR6sul58cTpPtkXh_e4uPq0uWzOyouvn8-b9UVpBMFTiYWtrODaWSMZpppL7rgz0mXb1oJZXBlZE3BECAqsbZnAVnMmqpZUW8HpcfFxn3szbwfb7nfo1U30g453Kmiv_nZG36ld-KFIzYEJmQPeHQJi-D7bNKnBJ2P7Xo82zElJzDnLIP4vknIuWSbf_ENehzmO-Q6q4qKCihGRofd7yMSQUrTufmgMaqldrTdf1K_as5LxV38ueg__7jkDbw-ATkb3LvdsfHrgKJFcQv0wXJd7v_XRKp0Glb-YqoTCtRJMLGGv95DTQeldzEFX3whgCjjXQQjQn91Ayo8</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Shan, Huifeng</creator><creator>Kurtz, Harry D. 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Hemisynthesis</topic><topic>Biodegradation</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotransformation</topic><topic>Carbon Dioxide - metabolism</topic><topic>Carbon Monoxide - metabolism</topic><topic>Carboxylic Acids - metabolism</topic><topic>Chlorine - metabolism</topic><topic>Chloroform - metabolism</topic><topic>Culture Media - chemistry</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fatty Acids - analysis</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Pantoea - classification</topic><topic>Pantoea - growth & development</topic><topic>Pantoea - isolation & purification</topic><topic>Pantoea - metabolism</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Soil Microbiology</topic><topic>Vitamin B</topic><topic>Vitamin B 12 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shan, Huifeng</creatorcontrib><creatorcontrib>Kurtz, Harry D. 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Jr</au><au>Mykytczuk, Nadia</au><au>Trevors, Jack T</au><au>Freedman, David L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>76</volume><issue>19</issue><spage>6463</spage><epage>6469</epage><pages>6463-6469</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two Pantoea spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to increasing levels of CF, corn syrup-grown DHM-1 was able to transform over 500 mg/liter CF in the presence of vitamin B₁₂ (approximately 3% of CF on a molar basis) at a rate as high as 22 mg/liter/day in a mineral salts medium. CO, CO₂, and organic acids were the predominant biodegradation products, suggesting that hydrolytic reactions predominate during CF transformation. DHM-1 was capable of growing on corn syrup in the presence of high concentrations of CF (as may be present near contaminant source zones in groundwater), which makes it a promising culture for bioaugmentation. Strains DHM-1B and DHM-1T transform CF at rates similar to that of the DHM-1 enrichment culture. The ability of these strains to grow in the presence of high concentrations of CF appears to be related to alteration of membrane fluidity or homeoviscous and homeophasic adaptation.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20693443</pmid><doi>10.1128/aem.01191-10</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acclimation Anaerobiosis Bacteria Bioconversions. Hemisynthesis Biodegradation Biological and medical sciences Biotechnology Biotransformation Carbon Dioxide - metabolism Carbon Monoxide - metabolism Carboxylic Acids - metabolism Chlorine - metabolism Chloroform - metabolism Culture Media - chemistry DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fatty Acids - analysis Fermentation Fundamental and applied biological sciences. Psychology Metabolism Methods. Procedures. Technologies Microbiology Molecular Sequence Data Pantoea - classification Pantoea - growth & development Pantoea - isolation & purification Pantoea - metabolism RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Soil Microbiology Vitamin B Vitamin B 12 - metabolism |
| title | Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates |
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