Use of an Escherichia coli Recombinant Producing Thermostable Polyphosphate Kinase as an ATP Regenerator To Produce Fructose 1,6-Diphosphate

Use of an Escherichia coli Recombinant Producing Thermostable Polyphosphate Kinase as an ATP Regenerator To Produce Fructose 1,6-Diphosphate

Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrate...

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Veröffentlicht in:Applied and Environmental Microbiology 2007-09, Vol.73 (17), p.5676-5678
Hauptverfasser: Iwamoto, Seishi, Motomura, Kei, Shinoda, Yasuharu, Urata, Masaaki, Kato, Junichi, Takiguchi, Noboru, Ohtake, Hisao, Hirota, Ryuichi, Kuroda, Akio
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine triphosphatase
Adenosine Triphosphate - biosynthesis
Biological and medical sciences
Biotechnology - methods
E coli
Enzyme Stability
Enzymes
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fructose - metabolism
Fructosediphosphates - biosynthesis
Fundamental and applied biological sciences. Psychology
Hot Temperature
Kinases
Microbiology
Phosphotransferases (Phosphate Group Acceptor) - genetics
Phosphotransferases (Phosphate Group Acceptor) - metabolism
Physiology and Biotechnology
Polyphosphates - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Thermus
Thermus thermophilus - enzymology
Thermus thermophilus - genetics
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Zusammenfassung:Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.00278-07