Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA

A quantitative molecular technique was developed for rapid analysis of microbial community diversity in various environments. The technique employed PCR in which one of the two primers used was fluorescently labeled at the 5' end and was used to amplify a selected region of bacterial genes enco...

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Veröffentlicht in:	Applied and Environmental Microbiology 1997-11, Vol.63 (11), p.4516-4522
Hauptverfasser:	Liu, W.T, Marsh, T.L, Cheng, H, Forney, L.J
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN analysis Animal, plant and microbial ecology ARN RIBOSOMAL ARN RIBOSOMIAL BACTERIA BACTERIAL COMMUNITIES Biodiversity Biological and medical sciences DNA DNA, Bacterial DNA, Bacterial - analysis DNA, Ribosomal DNA, Ribosomal - analysis FLORA MICROBIANA FLORE MICROBIENNE Fluorescence Fundamental and applied biological sciences. Psychology General aspects. Techniques Genes Genetic Variation genetics groundwater contamination GROUNDWATER POLLUTION INTESTIN intestinal microorganisms INTESTINES INTESTINOS Methods and techniques (sampling, tagging, trapping, modelling...) MICROBIAL FLORA Microbiology PCR POLLUTED WATER POLLUTION DE L'EAU POLLUTION DE L'EAU SOUTERRAINE POLUCION DE AGUAS SUBTERRANEAS POLUCION DEL AGUA POLYMERASE CHAIN REACTION Polymorphism, Restriction Fragment Length RETICULITERMES FLAVIPES Ribonucleic acid ribosomal DNA RIBOSOMAL RNA RIBOSOMAS RIBOSOME RIBOSOMES RNA RNA, Ribosomal, 16S RNA, Ribosomal, 16S - genetics WATER POLLUTION
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