Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes
This study was conducted to systematically assess the reproductive performance of transgenic TLR4 ewes. In the TLR4 transgenic founders (F 0) and their positive offspring (F 1), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed....
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| Veröffentlicht in: | Frontiers of Agricultural Science and Engineering 2014-12, Vol.1 (4), p.314-320 |
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| container_title | Frontiers of Agricultural Science and Engineering |
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| creator | FANG, Yi FU, Xiangwei LI, Junjie DU, Ming JIA, Baoyu ZHANG, Jinlong ZHANG, Xiaosheng ZHU, Shien |
| description | This study was conducted to systematically assess the reproductive performance of transgenic TLR4 ewes. In the TLR4 transgenic founders (F 0) and their positive offspring (F 1), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep. |
| doi_str_mv | 10.15302/J-FASE-2014038 |
| format | Article |
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In the TLR4 transgenic founders (F 0) and their positive offspring (F 1), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep.</description><identifier>ISSN: 2095-7505</identifier><identifier>EISSN: 2095-977X</identifier><identifier>DOI: 10.15302/J-FASE-2014038</identifier><language>eng</language><publisher>Higher Education Press</publisher><subject>DNA methylation ; oocyte ; reproductive trait ; safety assessment ; TLR4 transgenic ewe ; TLR4 transgenic ewe|safety assessment|reproductive trait|oocyte|DNA methylation</subject><ispartof>Frontiers of Agricultural Science and Engineering, 2014-12, Vol.1 (4), p.314-320</ispartof><rights>Copyright reserved, 2014, Higher Education Press and Springer-Verlag Berlin Heidelberg</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-13ce3e6a84df598b9c4874388d196887193da8b564f9eacb5383eff7813241533</citedby><cites>FETCH-LOGICAL-c441t-13ce3e6a84df598b9c4874388d196887193da8b564f9eacb5383eff7813241533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>FANG, Yi</creatorcontrib><creatorcontrib>FU, Xiangwei</creatorcontrib><creatorcontrib>LI, Junjie</creatorcontrib><creatorcontrib>DU, Ming</creatorcontrib><creatorcontrib>JIA, Baoyu</creatorcontrib><creatorcontrib>ZHANG, Jinlong</creatorcontrib><creatorcontrib>ZHANG, Xiaosheng</creatorcontrib><creatorcontrib>ZHU, Shien</creatorcontrib><title>Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes</title><title>Frontiers of Agricultural Science and Engineering</title><addtitle>Front. Agr. Sci. Eng</addtitle><description>This study was conducted to systematically assess the reproductive performance of transgenic TLR4 ewes. In the TLR4 transgenic founders (F 0) and their positive offspring (F 1), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep.</description><subject>DNA methylation</subject><subject>oocyte</subject><subject>reproductive trait</subject><subject>safety assessment</subject><subject>TLR4 transgenic ewe</subject><subject>TLR4 transgenic ewe|safety assessment|reproductive trait|oocyte|DNA methylation</subject><issn>2095-7505</issn><issn>2095-977X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp1kE1PAjEQhjdGEwl69to_sNJu2217JAgKIZr4kXhrut0p1MCWtFXDv3cR4s3LzGRm3ufwFMUNwbeEU1yNFuVs_DItK0wYpvKsGFRY8VIJ8X5-mgXH_LK4TukDY0xEX7AcFHbqHNicUHAorwG9Lp8ZytF0aQUdoNChCLsY2k-b_RccLr5_Nl2L7h7HaAt5vd-Y7Pu_nckZYncAhWD3GRLyHYJvSFfFhTObBNenPizeZtPXyUO5fLqfT8bL0jJGckmoBQq1kax1XMlGWSYFo1K2RNVSCqJoa2TDa-YUGNtwKik4JyShFest0GExP3LbYD70LvqtiXsdjNe_ixBX2sTs7QZ0w3BTy4ZS3HBmCUhVcSKMkFhwaLjqWaMjy8aQUgT3xyNY_yrXC31Qrk_K-0R9TKz9ag0R2l2ElLSLocseYvo3-AMqT4S2</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>FANG, Yi</creator><creator>FU, Xiangwei</creator><creator>LI, Junjie</creator><creator>DU, Ming</creator><creator>JIA, Baoyu</creator><creator>ZHANG, Jinlong</creator><creator>ZHANG, Xiaosheng</creator><creator>ZHU, Shien</creator><general>Higher Education Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>20141201</creationdate><title>Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes</title><author>FANG, Yi ; FU, Xiangwei ; LI, Junjie ; DU, Ming ; JIA, Baoyu ; ZHANG, Jinlong ; ZHANG, Xiaosheng ; ZHU, Shien</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-13ce3e6a84df598b9c4874388d196887193da8b564f9eacb5383eff7813241533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>DNA methylation</topic><topic>oocyte</topic><topic>reproductive trait</topic><topic>safety assessment</topic><topic>TLR4 transgenic ewe</topic><topic>TLR4 transgenic ewe|safety assessment|reproductive trait|oocyte|DNA methylation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FANG, Yi</creatorcontrib><creatorcontrib>FU, Xiangwei</creatorcontrib><creatorcontrib>LI, Junjie</creatorcontrib><creatorcontrib>DU, Ming</creatorcontrib><creatorcontrib>JIA, Baoyu</creatorcontrib><creatorcontrib>ZHANG, Jinlong</creatorcontrib><creatorcontrib>ZHANG, Xiaosheng</creatorcontrib><creatorcontrib>ZHU, Shien</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers of Agricultural Science and Engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FANG, Yi</au><au>FU, Xiangwei</au><au>LI, Junjie</au><au>DU, Ming</au><au>JIA, Baoyu</au><au>ZHANG, Jinlong</au><au>ZHANG, Xiaosheng</au><au>ZHU, Shien</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes</atitle><jtitle>Frontiers of Agricultural Science and Engineering</jtitle><stitle>Front. Agr. Sci. Eng</stitle><date>2014-12-01</date><risdate>2014</risdate><volume>1</volume><issue>4</issue><spage>314</spage><epage>320</epage><pages>314-320</pages><issn>2095-7505</issn><eissn>2095-977X</eissn><abstract>This study was conducted to systematically assess the reproductive performance of transgenic TLR4 ewes. In the TLR4 transgenic founders (F 0) and their positive offspring (F 1), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep.</abstract><pub>Higher Education Press</pub><doi>10.15302/J-FASE-2014038</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | DNA methylation oocyte reproductive trait safety assessment TLR4 transgenic ewe TLR4 transgenic ewe|safety assessment|reproductive trait|oocyte|DNA methylation |
| title | Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes |
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