The role of hepatitis C virus genotypes and core mutations in hepatocellular carcinoma in Cameroon
Background Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV‐related factors on HCC development still remains unknown in the Central Africa. In this study, we investigated the role of HCV genotypes and...
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| Veröffentlicht in: | Journal of viral hepatitis 2020-09, Vol.27 (9), p.880-885 |
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| container_title | Journal of viral hepatitis |
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| creator | Amougou‐Atsama, Marie Jean Adrien Atangana, Paul Noah Noah, Dominique Fewou Moundipa, Paul Pineau, Pascal Njouom, Richard |
| description | Background
Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV‐related factors on HCC development still remains unknown in the Central Africa. In this study, we investigated the role of HCV genotypes and core mutations in HCC development in Cameroonian patients.
Methods
A case‐control study was conducted using patients with HCV‐related HCC and matched controls individuals with chronic HCV infection but without HCC. HCV genotypes and mutations were determined using a hemi‐nested amplification and sequencing analysis focus on the core and NS5B HCV regions.
Results
We identify HCV genotype 1, 2 and 4 in both groups. Interestingly, genotype 4 was significantly more prevalent in HCC patients (53.3%). Overall, distribution of genotypes was very different between cases and controls (P = 4.2 E‐7). The risk factors analysis showed that infection with HCV‐4 is strongly associated with HCC development with odd ratio, 95% confidence interval and p‐values of 7.4 (95% CI: 2.08‐26.6; P = .001). Furthermore, the risk of developing HCC increased even more significantly in case of infection with HCV subtype 4f with the odd ratio of 20.8 (95% CI, 4.1‐66.8; P |
| doi_str_mv | 10.1111/jvh.13303 |
| format | Article |
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Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV‐related factors on HCC development still remains unknown in the Central Africa. In this study, we investigated the role of HCV genotypes and core mutations in HCC development in Cameroonian patients.
Methods
A case‐control study was conducted using patients with HCV‐related HCC and matched controls individuals with chronic HCV infection but without HCC. HCV genotypes and mutations were determined using a hemi‐nested amplification and sequencing analysis focus on the core and NS5B HCV regions.
Results
We identify HCV genotype 1, 2 and 4 in both groups. Interestingly, genotype 4 was significantly more prevalent in HCC patients (53.3%). Overall, distribution of genotypes was very different between cases and controls (P = 4.2 E‐7). The risk factors analysis showed that infection with HCV‐4 is strongly associated with HCC development with odd ratio, 95% confidence interval and p‐values of 7.4 (95% CI: 2.08‐26.6; P = .001). Furthermore, the risk of developing HCC increased even more significantly in case of infection with HCV subtype 4f with the odd ratio of 20.8 (95% CI, 4.1‐66.8; P < .001). Mutations K10R, T72E, K74R and G77A were significantly more frequent in patients with HCC. Remarkably, HCV‐4f isolates from HCC patients carried significantly more mutations when compared to controls with HCV‐4f or others genotypes (P = .0001).
Conclusions
Our results indicate that patients infected with HCV‐4f or with selected variants affecting HCV core gene are at increased risk to develop HCC.</description><identifier>ISSN: 1352-0504</identifier><identifier>EISSN: 1365-2893</identifier><identifier>DOI: 10.1111/jvh.13303</identifier><identifier>PMID: 32301239</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Cameroon ; Chronic infection ; core ; Genotype ; Genotypes ; Hepatitis ; Hepatitis C ; Hepatocellular carcinoma ; Infections ; Life Sciences ; Liver cancer ; Mutation ; mutations ; Risk factors ; Sequence analysis</subject><ispartof>Journal of viral hepatitis, 2020-09, Vol.27 (9), p.880-885</ispartof><rights>2020 John Wiley & Sons Ltd</rights><rights>2020 John Wiley & Sons Ltd.</rights><rights>Copyright © 2020 John Wiley & Sons Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3913-9b923e0e1b0d685348ab9498d194d4602183ef5ffa9059d79bfc5fa34cc2f7393</citedby><cites>FETCH-LOGICAL-c3913-9b923e0e1b0d685348ab9498d194d4602183ef5ffa9059d79bfc5fa34cc2f7393</cites><orcidid>0000-0002-9407-1592 ; 0000-0003-3112-6370</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjvh.13303$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjvh.13303$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32301239$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://pasteur.hal.science/pasteur-03244796$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Amougou‐Atsama, Marie</creatorcontrib><creatorcontrib>Jean Adrien Atangana, Paul</creatorcontrib><creatorcontrib>Noah Noah, Dominique</creatorcontrib><creatorcontrib>Fewou Moundipa, Paul</creatorcontrib><creatorcontrib>Pineau, Pascal</creatorcontrib><creatorcontrib>Njouom, Richard</creatorcontrib><title>The role of hepatitis C virus genotypes and core mutations in hepatocellular carcinoma in Cameroon</title><title>Journal of viral hepatitis</title><addtitle>J Viral Hepat</addtitle><description>Background
Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV‐related factors on HCC development still remains unknown in the Central Africa. In this study, we investigated the role of HCV genotypes and core mutations in HCC development in Cameroonian patients.
Methods
A case‐control study was conducted using patients with HCV‐related HCC and matched controls individuals with chronic HCV infection but without HCC. HCV genotypes and mutations were determined using a hemi‐nested amplification and sequencing analysis focus on the core and NS5B HCV regions.
Results
We identify HCV genotype 1, 2 and 4 in both groups. Interestingly, genotype 4 was significantly more prevalent in HCC patients (53.3%). Overall, distribution of genotypes was very different between cases and controls (P = 4.2 E‐7). The risk factors analysis showed that infection with HCV‐4 is strongly associated with HCC development with odd ratio, 95% confidence interval and p‐values of 7.4 (95% CI: 2.08‐26.6; P = .001). Furthermore, the risk of developing HCC increased even more significantly in case of infection with HCV subtype 4f with the odd ratio of 20.8 (95% CI, 4.1‐66.8; P < .001). Mutations K10R, T72E, K74R and G77A were significantly more frequent in patients with HCC. Remarkably, HCV‐4f isolates from HCC patients carried significantly more mutations when compared to controls with HCV‐4f or others genotypes (P = .0001).
Conclusions
Our results indicate that patients infected with HCV‐4f or with selected variants affecting HCV core gene are at increased risk to develop HCC.</description><subject>Cameroon</subject><subject>Chronic infection</subject><subject>core</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Hepatitis</subject><subject>Hepatitis C</subject><subject>Hepatocellular carcinoma</subject><subject>Infections</subject><subject>Life Sciences</subject><subject>Liver cancer</subject><subject>Mutation</subject><subject>mutations</subject><subject>Risk factors</subject><subject>Sequence analysis</subject><issn>1352-0504</issn><issn>1365-2893</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kE1v3CAQQFHVqvloD_kDEVJPPTgBBrzmGK3abKqVekl7RRgPXa9s44C91f77snGSW7mAxJun0SPkirMbns_t_rC74QAM3pFzDqUqRKXh_emtRMEUk2fkIqU9YxyE4h_JGQhgXIA-J_XjDmkMHdLg6Q5HO7VTm-iaHto4J_oHhzAdR0zUDg11ISLt5ylDYUi0HZaJ4LDr5s5G6mx07RB6e_pb2x5jCMMn8sHbLuHnl_uS_Pr-7XG9KbY_7x_Wd9vCgeZQ6FoLQIa8Zk1ZKZCVrbXUVcO1bGTJBK8AvfLeaqZ0s9K1d8pbkM4JvwINl6RYvDvbmTG2vY1HE2xrNndbM9o04RwNAyHlSpcHnvkvCz_G8DRjmsw-zHHIKxohc6Gyklxl6utCuRhSiujf1JyZU32T65vn-pm9fjHOdY_NG_maOwO3C_C37fD4f5P58XuzKP8BhTqN1A</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Amougou‐Atsama, Marie</creator><creator>Jean Adrien Atangana, Paul</creator><creator>Noah Noah, Dominique</creator><creator>Fewou Moundipa, Paul</creator><creator>Pineau, Pascal</creator><creator>Njouom, Richard</creator><general>Wiley Subscription Services, Inc</general><general>Wiley-Blackwell</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9407-1592</orcidid><orcidid>https://orcid.org/0000-0003-3112-6370</orcidid></search><sort><creationdate>202009</creationdate><title>The role of hepatitis C virus genotypes and core mutations in hepatocellular carcinoma in Cameroon</title><author>Amougou‐Atsama, Marie ; Jean Adrien Atangana, Paul ; Noah Noah, Dominique ; Fewou Moundipa, Paul ; Pineau, Pascal ; Njouom, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3913-9b923e0e1b0d685348ab9498d194d4602183ef5ffa9059d79bfc5fa34cc2f7393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cameroon</topic><topic>Chronic infection</topic><topic>core</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Hepatitis</topic><topic>Hepatitis C</topic><topic>Hepatocellular carcinoma</topic><topic>Infections</topic><topic>Life Sciences</topic><topic>Liver cancer</topic><topic>Mutation</topic><topic>mutations</topic><topic>Risk factors</topic><topic>Sequence analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amougou‐Atsama, Marie</creatorcontrib><creatorcontrib>Jean Adrien Atangana, Paul</creatorcontrib><creatorcontrib>Noah Noah, Dominique</creatorcontrib><creatorcontrib>Fewou Moundipa, Paul</creatorcontrib><creatorcontrib>Pineau, Pascal</creatorcontrib><creatorcontrib>Njouom, Richard</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of viral hepatitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amougou‐Atsama, Marie</au><au>Jean Adrien Atangana, Paul</au><au>Noah Noah, Dominique</au><au>Fewou Moundipa, Paul</au><au>Pineau, Pascal</au><au>Njouom, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of hepatitis C virus genotypes and core mutations in hepatocellular carcinoma in Cameroon</atitle><jtitle>Journal of viral hepatitis</jtitle><addtitle>J Viral Hepat</addtitle><date>2020-09</date><risdate>2020</risdate><volume>27</volume><issue>9</issue><spage>880</spage><epage>885</epage><pages>880-885</pages><issn>1352-0504</issn><eissn>1365-2893</eissn><abstract>Background
Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV‐related factors on HCC development still remains unknown in the Central Africa. In this study, we investigated the role of HCV genotypes and core mutations in HCC development in Cameroonian patients.
Methods
A case‐control study was conducted using patients with HCV‐related HCC and matched controls individuals with chronic HCV infection but without HCC. HCV genotypes and mutations were determined using a hemi‐nested amplification and sequencing analysis focus on the core and NS5B HCV regions.
Results
We identify HCV genotype 1, 2 and 4 in both groups. Interestingly, genotype 4 was significantly more prevalent in HCC patients (53.3%). Overall, distribution of genotypes was very different between cases and controls (P = 4.2 E‐7). The risk factors analysis showed that infection with HCV‐4 is strongly associated with HCC development with odd ratio, 95% confidence interval and p‐values of 7.4 (95% CI: 2.08‐26.6; P = .001). Furthermore, the risk of developing HCC increased even more significantly in case of infection with HCV subtype 4f with the odd ratio of 20.8 (95% CI, 4.1‐66.8; P < .001). Mutations K10R, T72E, K74R and G77A were significantly more frequent in patients with HCC. Remarkably, HCV‐4f isolates from HCC patients carried significantly more mutations when compared to controls with HCV‐4f or others genotypes (P = .0001).
Conclusions
Our results indicate that patients infected with HCV‐4f or with selected variants affecting HCV core gene are at increased risk to develop HCC.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32301239</pmid><doi>10.1111/jvh.13303</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-9407-1592</orcidid><orcidid>https://orcid.org/0000-0003-3112-6370</orcidid></addata></record> |
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| source | Wiley Online Library All Journals |
| subjects | Cameroon Chronic infection core Genotype Genotypes Hepatitis Hepatitis C Hepatocellular carcinoma Infections Life Sciences Liver cancer Mutation mutations Risk factors Sequence analysis |
| title | The role of hepatitis C virus genotypes and core mutations in hepatocellular carcinoma in Cameroon |
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