α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading
Recent evidence suggests that disease progression in Parkinson’s disease (PD) could occur by the spreading of α-synuclein (α-syn) aggregates between neurons. Here we studied the role of astrocytes in the intercellular transfer and fate of α-syn fibrils, using in vitro and ex vivo models. α-Syn fibri...
Gespeichert in:
| Veröffentlicht in: | Acta neuropathologica 2017-11, Vol.134 (5), p.789-808 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 808 |
|---|---|
| container_issue | 5 |
| container_start_page | 789 |
| container_title | Acta neuropathologica |
| container_volume | 134 |
| creator | Loria, Frida Vargas, Jessica Y. Bousset, Luc Syan, Sylvie Salles, Audrey Melki, Ronald Zurzolo, Chiara |
| description | Recent evidence suggests that disease progression in Parkinson’s disease (PD) could occur by the spreading of α-synuclein (α-syn) aggregates between neurons. Here we studied the role of astrocytes in the intercellular transfer and fate of α-syn fibrils, using in vitro and ex vivo models. α-Syn fibrils can be transferred to neighboring cells; however, the transfer efficiency changes depending on the cell types. We found that α-syn is efficiently transferred from astrocytes to astrocytes and from neurons to astrocytes, but less efficiently from astrocytes to neurons. Interestingly, α-syn puncta are mainly found inside the lysosomal compartments of the recipient cells. However, differently from neurons, astrocytes are able to efficiently degrade fibrillar α-syn, suggesting an active role for these cells in clearing α-syn deposits. Astrocytes co-cultured with organotypic brain slices are able to take up α-syn fibrils from the slices. Altogether our data support a role for astrocytes in trapping and clearing α-syn pathological deposits in PD. |
| doi_str_mv | 10.1007/s00401-017-1746-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_pasteur_01568774v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1951914593</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-2045d3a7f5d7a2894132236943f76a9562a4467a0e8891c950815f2580cae5743</originalsourceid><addsrcrecordid>eNp1kc9u1DAQxi0EokvhAbggS1x6Cfi_k2NVAUVaiQNwtqbJpJsq6wTbodorb8SL9JmYVUpVIXGyrfnN5_nmY-y1FO-kEP59FsIIWQnpK-mNq9QTtpFGq0pYrZ-yjRBUdVqpE_Yi5xt6KW_sc3aiaq9s4_yG_br7XX09xKUdcYi8JIi5x8SvsNwiRh5xSVPMHGLHIZc0tYeCmQ-xG1o43soOyuPKPMKBA0_TiETxDq8TdFCGKfIEZUfS1BGPpTwnhG6I1y_Zsx7GjK_uz1P2_eOHbxeX1fbLp88X59uqNVaXSgljOw2-t50HVTdGkjHtGqN776CxToExzoPAum5k21hRS9srW4sW0HqjT1m16u5gDHMa9pAOYYIhXJ5vw0weyGsQ0rrae_NTEn-28nOafiyYS9gPucVxhIjTkoNslKSF1q4m9O0_6M20pEhuiLKykcY2mii5Um2ack7YP0whRTgGGtZAaQgfjoEGRT1v7pWXqz12Dx1_EyRArQDtk5aJ6dHX_1X9A-5Bq4Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1951914593</pqid></control><display><type>article</type><title>α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Loria, Frida ; Vargas, Jessica Y. ; Bousset, Luc ; Syan, Sylvie ; Salles, Audrey ; Melki, Ronald ; Zurzolo, Chiara</creator><creatorcontrib>Loria, Frida ; Vargas, Jessica Y. ; Bousset, Luc ; Syan, Sylvie ; Salles, Audrey ; Melki, Ronald ; Zurzolo, Chiara</creatorcontrib><description>Recent evidence suggests that disease progression in Parkinson’s disease (PD) could occur by the spreading of α-synuclein (α-syn) aggregates between neurons. Here we studied the role of astrocytes in the intercellular transfer and fate of α-syn fibrils, using in vitro and ex vivo models. α-Syn fibrils can be transferred to neighboring cells; however, the transfer efficiency changes depending on the cell types. We found that α-syn is efficiently transferred from astrocytes to astrocytes and from neurons to astrocytes, but less efficiently from astrocytes to neurons. Interestingly, α-syn puncta are mainly found inside the lysosomal compartments of the recipient cells. However, differently from neurons, astrocytes are able to efficiently degrade fibrillar α-syn, suggesting an active role for these cells in clearing α-syn deposits. Astrocytes co-cultured with organotypic brain slices are able to take up α-syn fibrils from the slices. Altogether our data support a role for astrocytes in trapping and clearing α-syn pathological deposits in PD.</description><identifier>ISSN: 0001-6322</identifier><identifier>EISSN: 1432-0533</identifier><identifier>DOI: 10.1007/s00401-017-1746-2</identifier><identifier>PMID: 28725967</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>alpha-Synuclein - metabolism ; Animals ; Astrocytes ; Astrocytes - metabolism ; Astrocytes - pathology ; Brain slice preparation ; Cells, Cultured ; Cellular Biology ; Coculture Techniques ; Disease Progression ; Fibrils ; Hippocampus - metabolism ; Hippocampus - pathology ; Life Sciences ; Medicine ; Medicine & Public Health ; Mice ; Movement disorders ; Neurobiology ; Neurodegenerative diseases ; Neurons ; Neurons - metabolism ; Neurons - pathology ; Neurons and Cognition ; Neurosciences ; Original Paper ; Parkinson Disease - metabolism ; Parkinson Disease - pathology ; Parkinson's disease ; Pathology ; Spreading ; Synuclein</subject><ispartof>Acta neuropathologica, 2017-11, Vol.134 (5), p.789-808</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Acta Neuropathologica is a copyright of Springer, 2017.</rights><rights>Attribution - NonCommercial</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-2045d3a7f5d7a2894132236943f76a9562a4467a0e8891c950815f2580cae5743</citedby><cites>FETCH-LOGICAL-c453t-2045d3a7f5d7a2894132236943f76a9562a4467a0e8891c950815f2580cae5743</cites><orcidid>0000-0002-0433-4337 ; 0000-0001-6048-6602</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00401-017-1746-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00401-017-1746-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28725967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://pasteur.hal.science/pasteur-01568774$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Loria, Frida</creatorcontrib><creatorcontrib>Vargas, Jessica Y.</creatorcontrib><creatorcontrib>Bousset, Luc</creatorcontrib><creatorcontrib>Syan, Sylvie</creatorcontrib><creatorcontrib>Salles, Audrey</creatorcontrib><creatorcontrib>Melki, Ronald</creatorcontrib><creatorcontrib>Zurzolo, Chiara</creatorcontrib><title>α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading</title><title>Acta neuropathologica</title><addtitle>Acta Neuropathol</addtitle><addtitle>Acta Neuropathol</addtitle><description>Recent evidence suggests that disease progression in Parkinson’s disease (PD) could occur by the spreading of α-synuclein (α-syn) aggregates between neurons. Here we studied the role of astrocytes in the intercellular transfer and fate of α-syn fibrils, using in vitro and ex vivo models. α-Syn fibrils can be transferred to neighboring cells; however, the transfer efficiency changes depending on the cell types. We found that α-syn is efficiently transferred from astrocytes to astrocytes and from neurons to astrocytes, but less efficiently from astrocytes to neurons. Interestingly, α-syn puncta are mainly found inside the lysosomal compartments of the recipient cells. However, differently from neurons, astrocytes are able to efficiently degrade fibrillar α-syn, suggesting an active role for these cells in clearing α-syn deposits. Astrocytes co-cultured with organotypic brain slices are able to take up α-syn fibrils from the slices. Altogether our data support a role for astrocytes in trapping and clearing α-syn pathological deposits in PD.</description><subject>alpha-Synuclein - metabolism</subject><subject>Animals</subject><subject>Astrocytes</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - pathology</subject><subject>Brain slice preparation</subject><subject>Cells, Cultured</subject><subject>Cellular Biology</subject><subject>Coculture Techniques</subject><subject>Disease Progression</subject><subject>Fibrils</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - pathology</subject><subject>Life Sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Movement disorders</subject><subject>Neurobiology</subject><subject>Neurodegenerative diseases</subject><subject>Neurons</subject><subject>Neurons - metabolism</subject><subject>Neurons - pathology</subject><subject>Neurons and Cognition</subject><subject>Neurosciences</subject><subject>Original Paper</subject><subject>Parkinson Disease - metabolism</subject><subject>Parkinson Disease - pathology</subject><subject>Parkinson's disease</subject><subject>Pathology</subject><subject>Spreading</subject><subject>Synuclein</subject><issn>0001-6322</issn><issn>1432-0533</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc9u1DAQxi0EokvhAbggS1x6Cfi_k2NVAUVaiQNwtqbJpJsq6wTbodorb8SL9JmYVUpVIXGyrfnN5_nmY-y1FO-kEP59FsIIWQnpK-mNq9QTtpFGq0pYrZ-yjRBUdVqpE_Yi5xt6KW_sc3aiaq9s4_yG_br7XX09xKUdcYi8JIi5x8SvsNwiRh5xSVPMHGLHIZc0tYeCmQ-xG1o43soOyuPKPMKBA0_TiETxDq8TdFCGKfIEZUfS1BGPpTwnhG6I1y_Zsx7GjK_uz1P2_eOHbxeX1fbLp88X59uqNVaXSgljOw2-t50HVTdGkjHtGqN776CxToExzoPAum5k21hRS9srW4sW0HqjT1m16u5gDHMa9pAOYYIhXJ5vw0weyGsQ0rrae_NTEn-28nOafiyYS9gPucVxhIjTkoNslKSF1q4m9O0_6M20pEhuiLKykcY2mii5Um2ack7YP0whRTgGGtZAaQgfjoEGRT1v7pWXqz12Dx1_EyRArQDtk5aJ6dHX_1X9A-5Bq4Q</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Loria, Frida</creator><creator>Vargas, Jessica Y.</creator><creator>Bousset, Luc</creator><creator>Syan, Sylvie</creator><creator>Salles, Audrey</creator><creator>Melki, Ronald</creator><creator>Zurzolo, Chiara</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-0433-4337</orcidid><orcidid>https://orcid.org/0000-0001-6048-6602</orcidid></search><sort><creationdate>20171101</creationdate><title>α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading</title><author>Loria, Frida ; Vargas, Jessica Y. ; Bousset, Luc ; Syan, Sylvie ; Salles, Audrey ; Melki, Ronald ; Zurzolo, Chiara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-2045d3a7f5d7a2894132236943f76a9562a4467a0e8891c950815f2580cae5743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>alpha-Synuclein - metabolism</topic><topic>Animals</topic><topic>Astrocytes</topic><topic>Astrocytes - metabolism</topic><topic>Astrocytes - pathology</topic><topic>Brain slice preparation</topic><topic>Cells, Cultured</topic><topic>Cellular Biology</topic><topic>Coculture Techniques</topic><topic>Disease Progression</topic><topic>Fibrils</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - pathology</topic><topic>Life Sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Movement disorders</topic><topic>Neurobiology</topic><topic>Neurodegenerative diseases</topic><topic>Neurons</topic><topic>Neurons - metabolism</topic><topic>Neurons - pathology</topic><topic>Neurons and Cognition</topic><topic>Neurosciences</topic><topic>Original Paper</topic><topic>Parkinson Disease - metabolism</topic><topic>Parkinson Disease - pathology</topic><topic>Parkinson's disease</topic><topic>Pathology</topic><topic>Spreading</topic><topic>Synuclein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loria, Frida</creatorcontrib><creatorcontrib>Vargas, Jessica Y.</creatorcontrib><creatorcontrib>Bousset, Luc</creatorcontrib><creatorcontrib>Syan, Sylvie</creatorcontrib><creatorcontrib>Salles, Audrey</creatorcontrib><creatorcontrib>Melki, Ronald</creatorcontrib><creatorcontrib>Zurzolo, Chiara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Acta neuropathologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loria, Frida</au><au>Vargas, Jessica Y.</au><au>Bousset, Luc</au><au>Syan, Sylvie</au><au>Salles, Audrey</au><au>Melki, Ronald</au><au>Zurzolo, Chiara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading</atitle><jtitle>Acta neuropathologica</jtitle><stitle>Acta Neuropathol</stitle><addtitle>Acta Neuropathol</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>134</volume><issue>5</issue><spage>789</spage><epage>808</epage><pages>789-808</pages><issn>0001-6322</issn><eissn>1432-0533</eissn><abstract>Recent evidence suggests that disease progression in Parkinson’s disease (PD) could occur by the spreading of α-synuclein (α-syn) aggregates between neurons. Here we studied the role of astrocytes in the intercellular transfer and fate of α-syn fibrils, using in vitro and ex vivo models. α-Syn fibrils can be transferred to neighboring cells; however, the transfer efficiency changes depending on the cell types. We found that α-syn is efficiently transferred from astrocytes to astrocytes and from neurons to astrocytes, but less efficiently from astrocytes to neurons. Interestingly, α-syn puncta are mainly found inside the lysosomal compartments of the recipient cells. However, differently from neurons, astrocytes are able to efficiently degrade fibrillar α-syn, suggesting an active role for these cells in clearing α-syn deposits. Astrocytes co-cultured with organotypic brain slices are able to take up α-syn fibrils from the slices. Altogether our data support a role for astrocytes in trapping and clearing α-syn pathological deposits in PD.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28725967</pmid><doi>10.1007/s00401-017-1746-2</doi><tpages>20</tpages><orcidid>https://orcid.org/0000-0002-0433-4337</orcidid><orcidid>https://orcid.org/0000-0001-6048-6602</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0001-6322 |
| ispartof | Acta neuropathologica, 2017-11, Vol.134 (5), p.789-808 |
| issn | 0001-6322 1432-0533 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_pasteur_01568774v1 |
| source | MEDLINE; SpringerLink Journals |
| subjects | alpha-Synuclein - metabolism Animals Astrocytes Astrocytes - metabolism Astrocytes - pathology Brain slice preparation Cells, Cultured Cellular Biology Coculture Techniques Disease Progression Fibrils Hippocampus - metabolism Hippocampus - pathology Life Sciences Medicine Medicine & Public Health Mice Movement disorders Neurobiology Neurodegenerative diseases Neurons Neurons - metabolism Neurons - pathology Neurons and Cognition Neurosciences Original Paper Parkinson Disease - metabolism Parkinson Disease - pathology Parkinson's disease Pathology Spreading Synuclein |
| title | α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T05%3A30%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%B1-Synuclein%20transfer%20between%20neurons%20and%20astrocytes%20indicates%20that%20astrocytes%20play%20a%20role%20in%20degradation%20rather%20than%20in%20spreading&rft.jtitle=Acta%20neuropathologica&rft.au=Loria,%20Frida&rft.date=2017-11-01&rft.volume=134&rft.issue=5&rft.spage=789&rft.epage=808&rft.pages=789-808&rft.issn=0001-6322&rft.eissn=1432-0533&rft_id=info:doi/10.1007/s00401-017-1746-2&rft_dat=%3Cproquest_hal_p%3E1951914593%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1951914593&rft_id=info:pmid/28725967&rfr_iscdi=true |