MALDI-TOF MS-based drug susceptibility testing of pathogens: The example of Candida albicans and fluconazole

MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The me...

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Veröffentlicht in:	Proteomics (Weinheim) 2009-10, Vol.9 (20), p.4627-4631
Hauptverfasser:	Marinach, Carine, Alanio, Alexandre, Palous, Martine, Kwasek, Stéphanie, Fekkar, Arnaud, Brossas, Jean-Yves, Brun, Sophie, Snounou, Georges, Hennequin, Christophe, Sanglard, Dominique, Datry, Annick, Golmard, Jean-Louis, Mazier, Dominique
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Sprache:	eng
Schlagworte:	Algorithm Antifungal Agents - pharmacology Candida albicans Candida albicans - drug effects Candida albicans - growth & development Fluconazole - pharmacology Life Sciences MALDI-TOF MS Microbial Sensitivity Tests - methods Microbiology Proteome profiling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Target for drugs
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creator	Marinach, Carine Alanio, Alexandre Palous, Martine Kwasek, Stéphanie Fekkar, Arnaud Brossas, Jean-Yves Brun, Sophie Snounou, Georges Hennequin, Christophe Sanglard, Dominique Datry, Annick Golmard, Jean-Louis Mazier, Dominique
description	MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute's reference method. This proof-of-concept demonstration highlights the potential for this approach to test other pathogens.
doi_str_mv	10.1002/pmic.200900152
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We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute's reference method. 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subjects	Algorithm Antifungal Agents - pharmacology Candida albicans Candida albicans - drug effects Candida albicans - growth & development Fluconazole - pharmacology Life Sciences MALDI-TOF MS Microbial Sensitivity Tests - methods Microbiology Proteome profiling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Target for drugs
title	MALDI-TOF MS-based drug susceptibility testing of pathogens: The example of Candida albicans and fluconazole
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