The combined effect of nisin, moderate heating and high hydrostatic pressure on the inactivation of Bacillus sporothermodurans spores
Aims To investigate the combined effect of hydrostatic pressure (HP), moderate temperature and nisin on the inactivation of Bacillus sporothermodurans spores which are known to be contaminant of dairy products and to be extremely heat‐resistant. Methods and Results A central composite experimental d...
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| Veröffentlicht in: | Journal of applied microbiology 2013-07, Vol.115 (1), p.147-155 |
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| creator | Aouadhi, C. Simonin, H. Mejri, S. Maaroufi, A. |
| description | Aims
To investigate the combined effect of hydrostatic pressure (HP), moderate temperature and nisin on the inactivation of Bacillus sporothermodurans spores which are known to be contaminant of dairy products and to be extremely heat‐resistant.
Methods and Results
A central composite experimental design with three factors, using response surface methodology, was used. By analysing the response surfaces and their corresponding contour plots, an interesting interaction with the three factors was observed. The inactivation observed was shown to be well fitted with values predicted by the quadratic equation, since the adjusted determination coefficient (Radj2) was 0·979. The optimum process parameters for a 5‐log spores ml−1 reduction of B. sporothermodurans spores were obtained, 472 MPa/53°C for 5 min in presence of 121 UI ml−1 of nisin.
Conclusion
Nisin and temperature treatments improve the effectiveness of pressure in the inactivation of highly heat‐resistant spores of B. sporothermodurans.
Significance and Impact of the Study
This study shows the potential of using high HP for a short time (5 min) in combination with moderate temperature and nisin to inactivate B. sporothermodurans spores in milk. Such treatments could be applied by the dairy industry to ensure the commercial sterility of UHT milk. |
| doi_str_mv | 10.1111/jam.12220 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_pasteur_00997849v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1367879563</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4540-245b8b49336153648a7c51fae79c5e85398a3719f46abec2384b584be7dc6ffe3</originalsourceid><addsrcrecordid>eNqNkW1r1TAUx4sobk5f-AUkIAMFuyVpHpqX16FOueKb-Tqk6ema0TbXpJ3cD-D39rheJwiCgZDDyY__efgXxXNGzxie8xs3njHOOX1QHLNKyZIrzR_exaKUVPOj4knON5Syikr1uDjilWKMS3Zc_Ljqgfg4NmGClkDXgZ9J7MgUcpjekDG2kNwMpAc3h-mauKklfbjuSb9vU8wzZj3ZJch5SUDiRGbUC5Pzc7jFP0yg2FvnwzAsmeRdTBGJhLpLctOagfy0eNS5IcOzw3tSfH3_7uristx--fDxYrMtvZCCllzIpm6EqbB9ibPVTnvJOgfaeAm1rEztKs1MJ5RrwPOqFo3EC7r1CkerTopy1e3dYHcpjC7tbXTBXm62dufyDEuylBqja2FuGfKvVn6X4rcF8mzHkD0Mg5sgLtmyyhjDFOfqP1Cla22kqhB9-Rd6E5c04eCWCa5riqoCqdcr5XHPOUF33zCj9pftFm23d7Yj--KguDQjtPfkb58ROD0ALns3dLh7H_IfTgtZU2WQO1-572GA_b8r2k-bz2vpn9mmw60</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1427803994</pqid></control><display><type>article</type><title>The combined effect of nisin, moderate heating and high hydrostatic pressure on the inactivation of Bacillus sporothermodurans spores</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Aouadhi, C. ; Simonin, H. ; Mejri, S. ; Maaroufi, A.</creator><creatorcontrib>Aouadhi, C. ; Simonin, H. ; Mejri, S. ; Maaroufi, A.</creatorcontrib><description>Aims
To investigate the combined effect of hydrostatic pressure (HP), moderate temperature and nisin on the inactivation of Bacillus sporothermodurans spores which are known to be contaminant of dairy products and to be extremely heat‐resistant.
Methods and Results
A central composite experimental design with three factors, using response surface methodology, was used. By analysing the response surfaces and their corresponding contour plots, an interesting interaction with the three factors was observed. The inactivation observed was shown to be well fitted with values predicted by the quadratic equation, since the adjusted determination coefficient (Radj2) was 0·979. The optimum process parameters for a 5‐log spores ml−1 reduction of B. sporothermodurans spores were obtained, 472 MPa/53°C for 5 min in presence of 121 UI ml−1 of nisin.
Conclusion
Nisin and temperature treatments improve the effectiveness of pressure in the inactivation of highly heat‐resistant spores of B. sporothermodurans.
Significance and Impact of the Study
This study shows the potential of using high HP for a short time (5 min) in combination with moderate temperature and nisin to inactivate B. sporothermodurans spores in milk. Such treatments could be applied by the dairy industry to ensure the commercial sterility of UHT milk.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12220</identifier><identifier>PMID: 23611251</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Anti-Bacterial Agents ; Anti-Bacterial Agents - pharmacology ; Bacillus ; Bacillus - drug effects ; Bacillus sporothermodurans ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; high pressure ; Hot Temperature ; Hydrostatic Pressure ; Life Sciences ; Microbial Viability ; Microbial Viability - drug effects ; Microbiology ; Nisin ; Nisin - pharmacology ; response surface methodology ; spore ; Spores, Bacterial ; Spores, Bacterial - drug effects ; temperature</subject><ispartof>Journal of applied microbiology, 2013-07, Vol.115 (1), p.147-155</ispartof><rights>Journal of Applied Microbiology © 2013 The Society for Applied Microbiology</rights><rights>2014 INIST-CNRS</rights><rights>Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.</rights><rights>Copyright © 2013 The Society for Applied Microbiology</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4540-245b8b49336153648a7c51fae79c5e85398a3719f46abec2384b584be7dc6ffe3</citedby><cites>FETCH-LOGICAL-c4540-245b8b49336153648a7c51fae79c5e85398a3719f46abec2384b584be7dc6ffe3</cites><orcidid>0000-0002-0933-2066 ; 0000-0002-5285-9728</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12220$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12220$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27458069$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23611251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://riip.hal.science/pasteur-00997849$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Aouadhi, C.</creatorcontrib><creatorcontrib>Simonin, H.</creatorcontrib><creatorcontrib>Mejri, S.</creatorcontrib><creatorcontrib>Maaroufi, A.</creatorcontrib><title>The combined effect of nisin, moderate heating and high hydrostatic pressure on the inactivation of Bacillus sporothermodurans spores</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To investigate the combined effect of hydrostatic pressure (HP), moderate temperature and nisin on the inactivation of Bacillus sporothermodurans spores which are known to be contaminant of dairy products and to be extremely heat‐resistant.
Methods and Results
A central composite experimental design with three factors, using response surface methodology, was used. By analysing the response surfaces and their corresponding contour plots, an interesting interaction with the three factors was observed. The inactivation observed was shown to be well fitted with values predicted by the quadratic equation, since the adjusted determination coefficient (Radj2) was 0·979. The optimum process parameters for a 5‐log spores ml−1 reduction of B. sporothermodurans spores were obtained, 472 MPa/53°C for 5 min in presence of 121 UI ml−1 of nisin.
Conclusion
Nisin and temperature treatments improve the effectiveness of pressure in the inactivation of highly heat‐resistant spores of B. sporothermodurans.
Significance and Impact of the Study
This study shows the potential of using high HP for a short time (5 min) in combination with moderate temperature and nisin to inactivate B. sporothermodurans spores in milk. Such treatments could be applied by the dairy industry to ensure the commercial sterility of UHT milk.</description><subject>Anti-Bacterial Agents</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacillus</subject><subject>Bacillus - drug effects</subject><subject>Bacillus sporothermodurans</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>high pressure</subject><subject>Hot Temperature</subject><subject>Hydrostatic Pressure</subject><subject>Life Sciences</subject><subject>Microbial Viability</subject><subject>Microbial Viability - drug effects</subject><subject>Microbiology</subject><subject>Nisin</subject><subject>Nisin - pharmacology</subject><subject>response surface methodology</subject><subject>spore</subject><subject>Spores, Bacterial</subject><subject>Spores, Bacterial - drug effects</subject><subject>temperature</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkW1r1TAUx4sobk5f-AUkIAMFuyVpHpqX16FOueKb-Tqk6ema0TbXpJ3cD-D39rheJwiCgZDDyY__efgXxXNGzxie8xs3njHOOX1QHLNKyZIrzR_exaKUVPOj4knON5Syikr1uDjilWKMS3Zc_Ljqgfg4NmGClkDXgZ9J7MgUcpjekDG2kNwMpAc3h-mauKklfbjuSb9vU8wzZj3ZJch5SUDiRGbUC5Pzc7jFP0yg2FvnwzAsmeRdTBGJhLpLctOagfy0eNS5IcOzw3tSfH3_7uristx--fDxYrMtvZCCllzIpm6EqbB9ibPVTnvJOgfaeAm1rEztKs1MJ5RrwPOqFo3EC7r1CkerTopy1e3dYHcpjC7tbXTBXm62dufyDEuylBqja2FuGfKvVn6X4rcF8mzHkD0Mg5sgLtmyyhjDFOfqP1Cla22kqhB9-Rd6E5c04eCWCa5riqoCqdcr5XHPOUF33zCj9pftFm23d7Yj--KguDQjtPfkb58ROD0ALns3dLh7H_IfTgtZU2WQO1-572GA_b8r2k-bz2vpn9mmw60</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Aouadhi, C.</creator><creator>Simonin, H.</creator><creator>Mejri, S.</creator><creator>Maaroufi, A.</creator><general>Blackwell</general><general>Oxford University Press</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-0933-2066</orcidid><orcidid>https://orcid.org/0000-0002-5285-9728</orcidid></search><sort><creationdate>201307</creationdate><title>The combined effect of nisin, moderate heating and high hydrostatic pressure on the inactivation of Bacillus sporothermodurans spores</title><author>Aouadhi, C. ; Simonin, H. ; Mejri, S. ; Maaroufi, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4540-245b8b49336153648a7c51fae79c5e85398a3719f46abec2384b584be7dc6ffe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anti-Bacterial Agents</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacillus</topic><topic>Bacillus - drug effects</topic><topic>Bacillus sporothermodurans</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>high pressure</topic><topic>Hot Temperature</topic><topic>Hydrostatic Pressure</topic><topic>Life Sciences</topic><topic>Microbial Viability</topic><topic>Microbial Viability - drug effects</topic><topic>Microbiology</topic><topic>Nisin</topic><topic>Nisin - pharmacology</topic><topic>response surface methodology</topic><topic>spore</topic><topic>Spores, Bacterial</topic><topic>Spores, Bacterial - drug effects</topic><topic>temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aouadhi, C.</creatorcontrib><creatorcontrib>Simonin, H.</creatorcontrib><creatorcontrib>Mejri, S.</creatorcontrib><creatorcontrib>Maaroufi, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aouadhi, C.</au><au>Simonin, H.</au><au>Mejri, S.</au><au>Maaroufi, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The combined effect of nisin, moderate heating and high hydrostatic pressure on the inactivation of Bacillus sporothermodurans spores</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2013-07</date><risdate>2013</risdate><volume>115</volume><issue>1</issue><spage>147</spage><epage>155</epage><pages>147-155</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
To investigate the combined effect of hydrostatic pressure (HP), moderate temperature and nisin on the inactivation of Bacillus sporothermodurans spores which are known to be contaminant of dairy products and to be extremely heat‐resistant.
Methods and Results
A central composite experimental design with three factors, using response surface methodology, was used. By analysing the response surfaces and their corresponding contour plots, an interesting interaction with the three factors was observed. The inactivation observed was shown to be well fitted with values predicted by the quadratic equation, since the adjusted determination coefficient (Radj2) was 0·979. The optimum process parameters for a 5‐log spores ml−1 reduction of B. sporothermodurans spores were obtained, 472 MPa/53°C for 5 min in presence of 121 UI ml−1 of nisin.
Conclusion
Nisin and temperature treatments improve the effectiveness of pressure in the inactivation of highly heat‐resistant spores of B. sporothermodurans.
Significance and Impact of the Study
This study shows the potential of using high HP for a short time (5 min) in combination with moderate temperature and nisin to inactivate B. sporothermodurans spores in milk. Such treatments could be applied by the dairy industry to ensure the commercial sterility of UHT milk.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>23611251</pmid><doi>10.1111/jam.12220</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0933-2066</orcidid><orcidid>https://orcid.org/0000-0002-5285-9728</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2013-07, Vol.115 (1), p.147-155 |
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| recordid | cdi_hal_primary_oai_HAL_pasteur_00997849v1 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anti-Bacterial Agents Anti-Bacterial Agents - pharmacology Bacillus Bacillus - drug effects Bacillus sporothermodurans Biological and medical sciences Fundamental and applied biological sciences. Psychology high pressure Hot Temperature Hydrostatic Pressure Life Sciences Microbial Viability Microbial Viability - drug effects Microbiology Nisin Nisin - pharmacology response surface methodology spore Spores, Bacterial Spores, Bacterial - drug effects temperature |
| title | The combined effect of nisin, moderate heating and high hydrostatic pressure on the inactivation of Bacillus sporothermodurans spores |
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