Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours
In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them...
Gespeichert in:
| Veröffentlicht in: | Acta biochimica Polonica 2011-01, Vol.58 (4), p.529-534 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 534 |
|---|---|
| container_issue | 4 |
| container_start_page | 529 |
| container_title | Acta biochimica Polonica |
| container_volume | 58 |
| creator | Piccinni, Eugenia Chelstowska, Anna Hanus, Jakub Widlak, Piotr Loreti, Simona Tata, Ada Maria Augusti-Tocco, Gabriella Bianchi, Michele Maria Negri, Rodolfo |
| description | In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them we found n-Myc transcription factor. The interaction between Gas41 and n-Myc was validated by pull-down experiments. We showed that Gas41 is able to bind both n-Myc and c-Myc proteins, and that the levels of expression of Gas41 and Myc proteins were similar to each other in such brain tumors as neuroblastomas and glioblastomas. Finally, in order to identify which region of Gas41 is involved in the interaction with Myc proteins, we analysed the ability of Gas41 to substitute for its orthologue Yaf9 in yeast; we showed that the N-terminal portions of the two proteins, containing the YEATS domains, are interchangeable, while the C-terminal portions are species-specific. In fact we found that Gas41 C-terminal portion is required for Myc protein interaction in human. |
| doi_str_mv | 10.18388/abp.2011_2220 |
| format | Article |
| fullrecord | <record><control><sourceid>hal_cross</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_pasteur_00978665v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_HAL_pasteur_00978665v1</sourcerecordid><originalsourceid>FETCH-LOGICAL-c340t-c2a542941e34a45b3c7760100fb2d5ad9d0247e19d451e7783ccd70826f18d343</originalsourceid><addsrcrecordid>eNo9kE1LAzEQQIMotlavHiV_YOtMkt1kj6VqK1S8KPQWsklWV7ofJLvC_nsXqz0NA-8NwyPkFmGJiit1b4puyQBRM8bgjMxRcpFgKvbnZA4AmKRM7mfkKsYvAMYxF5dkNqGZQlBzsn-ogrc9rZreB2P7qm1oW9KNiQKpaRx9GS31jW2dd7QYqam7Q1VW0_LhGx8njzY-fLdDpHGMva9pP9TtEOI1uSjNIfqbv7kg70-Pb-ttsnvdPK9Xu8RyAX1imUkFywV6LoxIC26lzAAByoK51LjcARPSY-5Eil5Kxa11EhTLSlSOC74gyfHupznoLlS1CaNuTaW3q53uzPTSEDRALlWWpd848csjb0MbY_DlSULQv0n1lFSfkk7C3VHohqL27oT_N-Q_SWhxPw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Piccinni, Eugenia ; Chelstowska, Anna ; Hanus, Jakub ; Widlak, Piotr ; Loreti, Simona ; Tata, Ada Maria ; Augusti-Tocco, Gabriella ; Bianchi, Michele Maria ; Negri, Rodolfo</creator><creatorcontrib>Piccinni, Eugenia ; Chelstowska, Anna ; Hanus, Jakub ; Widlak, Piotr ; Loreti, Simona ; Tata, Ada Maria ; Augusti-Tocco, Gabriella ; Bianchi, Michele Maria ; Negri, Rodolfo</creatorcontrib><description>In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them we found n-Myc transcription factor. The interaction between Gas41 and n-Myc was validated by pull-down experiments. We showed that Gas41 is able to bind both n-Myc and c-Myc proteins, and that the levels of expression of Gas41 and Myc proteins were similar to each other in such brain tumors as neuroblastomas and glioblastomas. Finally, in order to identify which region of Gas41 is involved in the interaction with Myc proteins, we analysed the ability of Gas41 to substitute for its orthologue Yaf9 in yeast; we showed that the N-terminal portions of the two proteins, containing the YEATS domains, are interchangeable, while the C-terminal portions are species-specific. In fact we found that Gas41 C-terminal portion is required for Myc protein interaction in human.</description><identifier>ISSN: 0001-527X</identifier><identifier>EISSN: 1734-154X</identifier><identifier>DOI: 10.18388/abp.2011_2220</identifier><identifier>PMID: 22068108</identifier><language>eng</language><publisher>Poland: Acta Biochimica Polonica</publisher><subject>Brain ; Brain - cytology ; Brain - metabolism ; Brain Neoplasms ; Brain Neoplasms - genetics ; Brain Neoplasms - metabolism ; Brain Neoplasms - pathology ; Cancer ; Cell Line, Tumor ; Cloning, Molecular ; Fetus ; Fetus - cytology ; Fetus - metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Gene Library ; Genes, Neoplasm ; Genetic Vectors ; Genetic Vectors - genetics ; Genetic Vectors - metabolism ; Glioblastoma ; Glioblastoma - genetics ; Glioblastoma - metabolism ; Glioblastoma - pathology ; Histone Acetyltransferases ; Histone Acetyltransferases - genetics ; Histone Acetyltransferases - metabolism ; Humans ; Life Sciences ; Neuroblastoma ; Neuroblastoma - genetics ; Neuroblastoma - metabolism ; Neuroblastoma - pathology ; Plasmids ; Plasmids - genetics ; Plasmids - metabolism ; Protein Interaction Domains and Motifs ; Protein Interaction Mapping ; Proto-Oncogene Proteins c-myc ; Proto-Oncogene Proteins c-myc - genetics ; Proto-Oncogene Proteins c-myc - metabolism ; Recombinant Fusion Proteins ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Silver Staining ; Species Specificity ; Transcription Factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Two-Hybrid System Techniques</subject><ispartof>Acta biochimica Polonica, 2011-01, Vol.58 (4), p.529-534</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-c2a542941e34a45b3c7760100fb2d5ad9d0247e19d451e7783ccd70826f18d343</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,860,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22068108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://riip.hal.science/pasteur-00978665$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Piccinni, Eugenia</creatorcontrib><creatorcontrib>Chelstowska, Anna</creatorcontrib><creatorcontrib>Hanus, Jakub</creatorcontrib><creatorcontrib>Widlak, Piotr</creatorcontrib><creatorcontrib>Loreti, Simona</creatorcontrib><creatorcontrib>Tata, Ada Maria</creatorcontrib><creatorcontrib>Augusti-Tocco, Gabriella</creatorcontrib><creatorcontrib>Bianchi, Michele Maria</creatorcontrib><creatorcontrib>Negri, Rodolfo</creatorcontrib><title>Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours</title><title>Acta biochimica Polonica</title><addtitle>Acta Biochim Pol</addtitle><description>In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them we found n-Myc transcription factor. The interaction between Gas41 and n-Myc was validated by pull-down experiments. We showed that Gas41 is able to bind both n-Myc and c-Myc proteins, and that the levels of expression of Gas41 and Myc proteins were similar to each other in such brain tumors as neuroblastomas and glioblastomas. Finally, in order to identify which region of Gas41 is involved in the interaction with Myc proteins, we analysed the ability of Gas41 to substitute for its orthologue Yaf9 in yeast; we showed that the N-terminal portions of the two proteins, containing the YEATS domains, are interchangeable, while the C-terminal portions are species-specific. In fact we found that Gas41 C-terminal portion is required for Myc protein interaction in human.</description><subject>Brain</subject><subject>Brain - cytology</subject><subject>Brain - metabolism</subject><subject>Brain Neoplasms</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - metabolism</subject><subject>Brain Neoplasms - pathology</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cloning, Molecular</subject><subject>Fetus</subject><subject>Fetus - cytology</subject><subject>Fetus - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Library</subject><subject>Genes, Neoplasm</subject><subject>Genetic Vectors</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>Glioblastoma</subject><subject>Glioblastoma - genetics</subject><subject>Glioblastoma - metabolism</subject><subject>Glioblastoma - pathology</subject><subject>Histone Acetyltransferases</subject><subject>Histone Acetyltransferases - genetics</subject><subject>Histone Acetyltransferases - metabolism</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Neuroblastoma</subject><subject>Neuroblastoma - genetics</subject><subject>Neuroblastoma - metabolism</subject><subject>Neuroblastoma - pathology</subject><subject>Plasmids</subject><subject>Plasmids - genetics</subject><subject>Plasmids - metabolism</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Protein Interaction Mapping</subject><subject>Proto-Oncogene Proteins c-myc</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>Recombinant Fusion Proteins</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Silver Staining</subject><subject>Species Specificity</subject><subject>Transcription Factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Two-Hybrid System Techniques</subject><issn>0001-527X</issn><issn>1734-154X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQQIMotlavHiV_YOtMkt1kj6VqK1S8KPQWsklWV7ofJLvC_nsXqz0NA-8NwyPkFmGJiit1b4puyQBRM8bgjMxRcpFgKvbnZA4AmKRM7mfkKsYvAMYxF5dkNqGZQlBzsn-ogrc9rZreB2P7qm1oW9KNiQKpaRx9GS31jW2dd7QYqam7Q1VW0_LhGx8njzY-fLdDpHGMva9pP9TtEOI1uSjNIfqbv7kg70-Pb-ttsnvdPK9Xu8RyAX1imUkFywV6LoxIC26lzAAByoK51LjcARPSY-5Eil5Kxa11EhTLSlSOC74gyfHupznoLlS1CaNuTaW3q53uzPTSEDRALlWWpd848csjb0MbY_DlSULQv0n1lFSfkk7C3VHohqL27oT_N-Q_SWhxPw</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Piccinni, Eugenia</creator><creator>Chelstowska, Anna</creator><creator>Hanus, Jakub</creator><creator>Widlak, Piotr</creator><creator>Loreti, Simona</creator><creator>Tata, Ada Maria</creator><creator>Augusti-Tocco, Gabriella</creator><creator>Bianchi, Michele Maria</creator><creator>Negri, Rodolfo</creator><general>Acta Biochimica Polonica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20110101</creationdate><title>Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours</title><author>Piccinni, Eugenia ; Chelstowska, Anna ; Hanus, Jakub ; Widlak, Piotr ; Loreti, Simona ; Tata, Ada Maria ; Augusti-Tocco, Gabriella ; Bianchi, Michele Maria ; Negri, Rodolfo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-c2a542941e34a45b3c7760100fb2d5ad9d0247e19d451e7783ccd70826f18d343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Brain</topic><topic>Brain - cytology</topic><topic>Brain - metabolism</topic><topic>Brain Neoplasms</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - metabolism</topic><topic>Brain Neoplasms - pathology</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cloning, Molecular</topic><topic>Fetus</topic><topic>Fetus - cytology</topic><topic>Fetus - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Library</topic><topic>Genes, Neoplasm</topic><topic>Genetic Vectors</topic><topic>Genetic Vectors - genetics</topic><topic>Genetic Vectors - metabolism</topic><topic>Glioblastoma</topic><topic>Glioblastoma - genetics</topic><topic>Glioblastoma - metabolism</topic><topic>Glioblastoma - pathology</topic><topic>Histone Acetyltransferases</topic><topic>Histone Acetyltransferases - genetics</topic><topic>Histone Acetyltransferases - metabolism</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Neuroblastoma</topic><topic>Neuroblastoma - genetics</topic><topic>Neuroblastoma - metabolism</topic><topic>Neuroblastoma - pathology</topic><topic>Plasmids</topic><topic>Plasmids - genetics</topic><topic>Plasmids - metabolism</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Protein Interaction Mapping</topic><topic>Proto-Oncogene Proteins c-myc</topic><topic>Proto-Oncogene Proteins c-myc - genetics</topic><topic>Proto-Oncogene Proteins c-myc - metabolism</topic><topic>Recombinant Fusion Proteins</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Silver Staining</topic><topic>Species Specificity</topic><topic>Transcription Factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Piccinni, Eugenia</creatorcontrib><creatorcontrib>Chelstowska, Anna</creatorcontrib><creatorcontrib>Hanus, Jakub</creatorcontrib><creatorcontrib>Widlak, Piotr</creatorcontrib><creatorcontrib>Loreti, Simona</creatorcontrib><creatorcontrib>Tata, Ada Maria</creatorcontrib><creatorcontrib>Augusti-Tocco, Gabriella</creatorcontrib><creatorcontrib>Bianchi, Michele Maria</creatorcontrib><creatorcontrib>Negri, Rodolfo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Acta biochimica Polonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Piccinni, Eugenia</au><au>Chelstowska, Anna</au><au>Hanus, Jakub</au><au>Widlak, Piotr</au><au>Loreti, Simona</au><au>Tata, Ada Maria</au><au>Augusti-Tocco, Gabriella</au><au>Bianchi, Michele Maria</au><au>Negri, Rodolfo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours</atitle><jtitle>Acta biochimica Polonica</jtitle><addtitle>Acta Biochim Pol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>58</volume><issue>4</issue><spage>529</spage><epage>534</epage><pages>529-534</pages><issn>0001-527X</issn><eissn>1734-154X</eissn><abstract>In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them we found n-Myc transcription factor. The interaction between Gas41 and n-Myc was validated by pull-down experiments. We showed that Gas41 is able to bind both n-Myc and c-Myc proteins, and that the levels of expression of Gas41 and Myc proteins were similar to each other in such brain tumors as neuroblastomas and glioblastomas. Finally, in order to identify which region of Gas41 is involved in the interaction with Myc proteins, we analysed the ability of Gas41 to substitute for its orthologue Yaf9 in yeast; we showed that the N-terminal portions of the two proteins, containing the YEATS domains, are interchangeable, while the C-terminal portions are species-specific. In fact we found that Gas41 C-terminal portion is required for Myc protein interaction in human.</abstract><cop>Poland</cop><pub>Acta Biochimica Polonica</pub><pmid>22068108</pmid><doi>10.18388/abp.2011_2220</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0001-527X |
| ispartof | Acta biochimica Polonica, 2011-01, Vol.58 (4), p.529-534 |
| issn | 0001-527X 1734-154X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_pasteur_00978665v1 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Brain Brain - cytology Brain - metabolism Brain Neoplasms Brain Neoplasms - genetics Brain Neoplasms - metabolism Brain Neoplasms - pathology Cancer Cell Line, Tumor Cloning, Molecular Fetus Fetus - cytology Fetus - metabolism Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Library Genes, Neoplasm Genetic Vectors Genetic Vectors - genetics Genetic Vectors - metabolism Glioblastoma Glioblastoma - genetics Glioblastoma - metabolism Glioblastoma - pathology Histone Acetyltransferases Histone Acetyltransferases - genetics Histone Acetyltransferases - metabolism Humans Life Sciences Neuroblastoma Neuroblastoma - genetics Neuroblastoma - metabolism Neuroblastoma - pathology Plasmids Plasmids - genetics Plasmids - metabolism Protein Interaction Domains and Motifs Protein Interaction Mapping Proto-Oncogene Proteins c-myc Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism Recombinant Fusion Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Silver Staining Species Specificity Transcription Factors Transcription Factors - genetics Transcription Factors - metabolism Two-Hybrid System Techniques |
| title | Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T04%3A40%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-hal_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20interaction%20of%20Gas41%20and%20Myc%20encoded%20by%20amplified%20genes%20in%20nervous%20system%20tumours&rft.jtitle=Acta%20biochimica%20Polonica&rft.au=Piccinni,%20Eugenia&rft.date=2011-01-01&rft.volume=58&rft.issue=4&rft.spage=529&rft.epage=534&rft.pages=529-534&rft.issn=0001-527X&rft.eissn=1734-154X&rft_id=info:doi/10.18388/abp.2011_2220&rft_dat=%3Chal_cross%3Eoai_HAL_pasteur_00978665v1%3C/hal_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/22068108&rfr_iscdi=true |