Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences

Methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. They have been shown previously to represent 50–70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The ISME Journal 2010-10, Vol.4 (10), p.1302-1313
Hauptverfasser: Auclair, Julie, Lépine, François, Parent, Serge, Villemur, Richard
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1313
container_issue 10
container_start_page 1302
container_title The ISME Journal
container_volume 4
creator Auclair, Julie
Lépine, François
Parent, Serge
Villemur, Richard
description Methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. They have been shown previously to represent 50–70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the Montreal Biodome. It was therefore surprising to find such a high concentration of Methylophaga spp. in anoxic conditions. In this study, we showed by cultivation-independent and -dependent approaches that one Methylophaga strain present in the anoxic biofilm is involved in the denitrification process. DNA stable-isotope probing (SIP) experiments in which the biofilm was cultured under denitrifying conditions with 13 C-methanol have revealed the enrichment of one particular taxon. By screening a 16S ribosomal RNA gene library derived from a 13 C-DNA fraction of the SIP gradients, 62% of the library was composed of one sequence affiliated with the genus Methylophaga . One strain, named JAM1, representing this Methylophaga species was isolated. It grows aerobically but also under denitrifying conditions by reducing nitrate into nitrite. The nitrate-reducing activity was correlated with the presence and the expression of two highly divergent narG genes ( narG 1 and narG 2). narG 1 showed a high percentage of identity with the corresponding part of narG found in Thiobacillus denitrificans, which suggests a recent acquisition of narG in strain JAM1 by horizontal gene transfer. This study provides the first direct evidence of the adaptation of a Methylophaga species to an oxygen-limited environment.
doi_str_mv 10.1038/ismej.2010.47
format Article
fullrecord <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_pasteur_00819611v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>755163680</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-1d365b5016eeeab2eafd50e25b40dc21b4fe026e00640b7d4aadf2313c93a3eb3</originalsourceid><addsrcrecordid>eNp9kUFv1DAQRiMEoqVw5IoscaCXLGM7sZNjVWiLtIgLnC0nmSReJfZiO1vl3-OyZUEIcZqx_PT5s16WvaawocCr9ybMuNswSOdCPsnOqSxpLrmEp6ddsLPsRQg7gFIKIZ9nZwx4zUvJzrPDBxOCmc2ko_Mr8dgtbTTOEtcTa6LXEYmxJKC-T6snzUo0-YxxXCe3H_WgSUhQIlpnY5rGDiTeOzKaYZxW0pkD-gFtJFb72xTzfUHbYniZPev1FPDV47zIvt18_Hp9l2-_3H66vtrmbSHqmNOOi7IpgQpE1A1D3XclICubArqW0aboEZhAAFFAI7tC665nnPK25ppjwy-y_Jg76kntvZm1X5XTRt1dbdVeh4iLVwAVrQWlB5r4d0d-712qGqKaTWhxmrRFtwQly5IKLipI5OV_SSopk6KSVZ3Qt3-hO7d4m_6tKLC6qkQy-btq610IHvtTXwrqQbX6qVo9qFaFTPybx9SlmbE70b_cJmBzBEK6sgP6P5_9V-IPsjm2LA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1029886103</pqid></control><display><type>article</type><title>Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences</title><source>MEDLINE</source><source>EZB Electronic Journals Library</source><source>Oxford Academic Journals (Open Access)</source><creator>Auclair, Julie ; Lépine, François ; Parent, Serge ; Villemur, Richard</creator><creatorcontrib>Auclair, Julie ; Lépine, François ; Parent, Serge ; Villemur, Richard</creatorcontrib><description>Methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. They have been shown previously to represent 50–70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the Montreal Biodome. It was therefore surprising to find such a high concentration of Methylophaga spp. in anoxic conditions. In this study, we showed by cultivation-independent and -dependent approaches that one Methylophaga strain present in the anoxic biofilm is involved in the denitrification process. DNA stable-isotope probing (SIP) experiments in which the biofilm was cultured under denitrifying conditions with 13 C-methanol have revealed the enrichment of one particular taxon. By screening a 16S ribosomal RNA gene library derived from a 13 C-DNA fraction of the SIP gradients, 62% of the library was composed of one sequence affiliated with the genus Methylophaga . One strain, named JAM1, representing this Methylophaga species was isolated. It grows aerobically but also under denitrifying conditions by reducing nitrate into nitrite. The nitrate-reducing activity was correlated with the presence and the expression of two highly divergent narG genes ( narG 1 and narG 2). narG 1 showed a high percentage of identity with the corresponding part of narG found in Thiobacillus denitrificans, which suggests a recent acquisition of narG in strain JAM1 by horizontal gene transfer. This study provides the first direct evidence of the adaptation of a Methylophaga species to an oxygen-limited environment.</description><identifier>ISSN: 1751-7362</identifier><identifier>EISSN: 1751-7370</identifier><identifier>DOI: 10.1038/ismej.2010.47</identifier><identifier>PMID: 20393572</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/208/325/2482 ; 631/326/41 ; 631/326/47 ; Anoxic conditions ; Aquariums ; Bacterial Proteins ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biofilms ; Biofilms - growth &amp; development ; Biomedical and Life Sciences ; Biotechnology ; Carbon Isotopes ; Carbon Isotopes - metabolism ; Cluster Analysis ; Computer Science ; Cultivation ; Denitrification ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Ecology ; Evolutionary Biology ; Life Sciences ; Marine environment ; Methanol ; Methanol - metabolism ; Methylophaga ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Molecular Sequence Data ; Nitrate Reductase ; Nitrate Reductase - genetics ; Nitrate Reductase - metabolism ; Nitrates ; Nitrates - metabolism ; Nitrites ; Nitrites - metabolism ; original-article ; Oxidation-Reduction ; Oxygen ; Oxygen - analysis ; Phylogeny ; Piscirickettsiaceae ; Piscirickettsiaceae - classification ; Piscirickettsiaceae - genetics ; Piscirickettsiaceae - isolation &amp; purification ; Piscirickettsiaceae - metabolism ; Reactors ; RNA, Ribosomal, 16S ; RNA, Ribosomal, 16S - genetics ; Seawater ; Seawater - chemistry ; Seawater - microbiology ; Sequence Analysis, DNA ; Staining and Labeling ; Staining and Labeling - methods ; Taxa ; Thiobacillus denitrificans</subject><ispartof>The ISME Journal, 2010-10, Vol.4 (10), p.1302-1313</ispartof><rights>International Society for Microbial Ecology 2010</rights><rights>Copyright Nature Publishing Group Oct 2010</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-1d365b5016eeeab2eafd50e25b40dc21b4fe026e00640b7d4aadf2313c93a3eb3</citedby><cites>FETCH-LOGICAL-c469t-1d365b5016eeeab2eafd50e25b40dc21b4fe026e00640b7d4aadf2313c93a3eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20393572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://riip.hal.science/pasteur-00819611$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Auclair, Julie</creatorcontrib><creatorcontrib>Lépine, François</creatorcontrib><creatorcontrib>Parent, Serge</creatorcontrib><creatorcontrib>Villemur, Richard</creatorcontrib><title>Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences</title><title>The ISME Journal</title><addtitle>ISME J</addtitle><addtitle>ISME J</addtitle><description>Methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. They have been shown previously to represent 50–70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the Montreal Biodome. It was therefore surprising to find such a high concentration of Methylophaga spp. in anoxic conditions. In this study, we showed by cultivation-independent and -dependent approaches that one Methylophaga strain present in the anoxic biofilm is involved in the denitrification process. DNA stable-isotope probing (SIP) experiments in which the biofilm was cultured under denitrifying conditions with 13 C-methanol have revealed the enrichment of one particular taxon. By screening a 16S ribosomal RNA gene library derived from a 13 C-DNA fraction of the SIP gradients, 62% of the library was composed of one sequence affiliated with the genus Methylophaga . One strain, named JAM1, representing this Methylophaga species was isolated. It grows aerobically but also under denitrifying conditions by reducing nitrate into nitrite. The nitrate-reducing activity was correlated with the presence and the expression of two highly divergent narG genes ( narG 1 and narG 2). narG 1 showed a high percentage of identity with the corresponding part of narG found in Thiobacillus denitrificans, which suggests a recent acquisition of narG in strain JAM1 by horizontal gene transfer. This study provides the first direct evidence of the adaptation of a Methylophaga species to an oxygen-limited environment.</description><subject>631/208/325/2482</subject><subject>631/326/41</subject><subject>631/326/47</subject><subject>Anoxic conditions</subject><subject>Aquariums</subject><subject>Bacterial Proteins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Biofilms - growth &amp; development</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbon Isotopes</subject><subject>Carbon Isotopes - metabolism</subject><subject>Cluster Analysis</subject><subject>Computer Science</subject><subject>Cultivation</subject><subject>Denitrification</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Life Sciences</subject><subject>Marine environment</subject><subject>Methanol</subject><subject>Methanol - metabolism</subject><subject>Methylophaga</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrate Reductase</subject><subject>Nitrate Reductase - genetics</subject><subject>Nitrate Reductase - metabolism</subject><subject>Nitrates</subject><subject>Nitrates - metabolism</subject><subject>Nitrites</subject><subject>Nitrites - metabolism</subject><subject>original-article</subject><subject>Oxidation-Reduction</subject><subject>Oxygen</subject><subject>Oxygen - analysis</subject><subject>Phylogeny</subject><subject>Piscirickettsiaceae</subject><subject>Piscirickettsiaceae - classification</subject><subject>Piscirickettsiaceae - genetics</subject><subject>Piscirickettsiaceae - isolation &amp; purification</subject><subject>Piscirickettsiaceae - metabolism</subject><subject>Reactors</subject><subject>RNA, Ribosomal, 16S</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Seawater</subject><subject>Seawater - chemistry</subject><subject>Seawater - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Staining and Labeling</subject><subject>Staining and Labeling - methods</subject><subject>Taxa</subject><subject>Thiobacillus denitrificans</subject><issn>1751-7362</issn><issn>1751-7370</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUFv1DAQRiMEoqVw5IoscaCXLGM7sZNjVWiLtIgLnC0nmSReJfZiO1vl3-OyZUEIcZqx_PT5s16WvaawocCr9ybMuNswSOdCPsnOqSxpLrmEp6ddsLPsRQg7gFIKIZ9nZwx4zUvJzrPDBxOCmc2ko_Mr8dgtbTTOEtcTa6LXEYmxJKC-T6snzUo0-YxxXCe3H_WgSUhQIlpnY5rGDiTeOzKaYZxW0pkD-gFtJFb72xTzfUHbYniZPev1FPDV47zIvt18_Hp9l2-_3H66vtrmbSHqmNOOi7IpgQpE1A1D3XclICubArqW0aboEZhAAFFAI7tC665nnPK25ppjwy-y_Jg76kntvZm1X5XTRt1dbdVeh4iLVwAVrQWlB5r4d0d-712qGqKaTWhxmrRFtwQly5IKLipI5OV_SSopk6KSVZ3Qt3-hO7d4m_6tKLC6qkQy-btq610IHvtTXwrqQbX6qVo9qFaFTPybx9SlmbE70b_cJmBzBEK6sgP6P5_9V-IPsjm2LA</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Auclair, Julie</creator><creator>Lépine, François</creator><creator>Parent, Serge</creator><creator>Villemur, Richard</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>SOI</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20101001</creationdate><title>Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences</title><author>Auclair, Julie ; Lépine, François ; Parent, Serge ; Villemur, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-1d365b5016eeeab2eafd50e25b40dc21b4fe026e00640b7d4aadf2313c93a3eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>631/208/325/2482</topic><topic>631/326/41</topic><topic>631/326/47</topic><topic>Anoxic conditions</topic><topic>Aquariums</topic><topic>Bacterial Proteins</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms</topic><topic>Biofilms - growth &amp; development</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Carbon Isotopes</topic><topic>Carbon Isotopes - metabolism</topic><topic>Cluster Analysis</topic><topic>Computer Science</topic><topic>Cultivation</topic><topic>Denitrification</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Life Sciences</topic><topic>Marine environment</topic><topic>Methanol</topic><topic>Methanol - metabolism</topic><topic>Methylophaga</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nitrate Reductase</topic><topic>Nitrate Reductase - genetics</topic><topic>Nitrate Reductase - metabolism</topic><topic>Nitrates</topic><topic>Nitrates - metabolism</topic><topic>Nitrites</topic><topic>Nitrites - metabolism</topic><topic>original-article</topic><topic>Oxidation-Reduction</topic><topic>Oxygen</topic><topic>Oxygen - analysis</topic><topic>Phylogeny</topic><topic>Piscirickettsiaceae</topic><topic>Piscirickettsiaceae - classification</topic><topic>Piscirickettsiaceae - genetics</topic><topic>Piscirickettsiaceae - isolation &amp; purification</topic><topic>Piscirickettsiaceae - metabolism</topic><topic>Reactors</topic><topic>RNA, Ribosomal, 16S</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Seawater</topic><topic>Seawater - chemistry</topic><topic>Seawater - microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Staining and Labeling</topic><topic>Staining and Labeling - methods</topic><topic>Taxa</topic><topic>Thiobacillus denitrificans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Auclair, Julie</creatorcontrib><creatorcontrib>Lépine, François</creatorcontrib><creatorcontrib>Parent, Serge</creatorcontrib><creatorcontrib>Villemur, Richard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The ISME Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Auclair, Julie</au><au>Lépine, François</au><au>Parent, Serge</au><au>Villemur, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences</atitle><jtitle>The ISME Journal</jtitle><stitle>ISME J</stitle><addtitle>ISME J</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>4</volume><issue>10</issue><spage>1302</spage><epage>1313</epage><pages>1302-1313</pages><issn>1751-7362</issn><eissn>1751-7370</eissn><abstract>Methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. They have been shown previously to represent 50–70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the Montreal Biodome. It was therefore surprising to find such a high concentration of Methylophaga spp. in anoxic conditions. In this study, we showed by cultivation-independent and -dependent approaches that one Methylophaga strain present in the anoxic biofilm is involved in the denitrification process. DNA stable-isotope probing (SIP) experiments in which the biofilm was cultured under denitrifying conditions with 13 C-methanol have revealed the enrichment of one particular taxon. By screening a 16S ribosomal RNA gene library derived from a 13 C-DNA fraction of the SIP gradients, 62% of the library was composed of one sequence affiliated with the genus Methylophaga . One strain, named JAM1, representing this Methylophaga species was isolated. It grows aerobically but also under denitrifying conditions by reducing nitrate into nitrite. The nitrate-reducing activity was correlated with the presence and the expression of two highly divergent narG genes ( narG 1 and narG 2). narG 1 showed a high percentage of identity with the corresponding part of narG found in Thiobacillus denitrificans, which suggests a recent acquisition of narG in strain JAM1 by horizontal gene transfer. This study provides the first direct evidence of the adaptation of a Methylophaga species to an oxygen-limited environment.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>20393572</pmid><doi>10.1038/ismej.2010.47</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1751-7362
ispartof The ISME Journal, 2010-10, Vol.4 (10), p.1302-1313
issn 1751-7362
1751-7370
language eng
recordid cdi_hal_primary_oai_HAL_pasteur_00819611v1
source MEDLINE; EZB Electronic Journals Library; Oxford Academic Journals (Open Access)
subjects 631/208/325/2482
631/326/41
631/326/47
Anoxic conditions
Aquariums
Bacterial Proteins
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofilms
Biofilms - growth & development
Biomedical and Life Sciences
Biotechnology
Carbon Isotopes
Carbon Isotopes - metabolism
Cluster Analysis
Computer Science
Cultivation
Denitrification
Deoxyribonucleic acid
DNA
DNA, Bacterial
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Ecology
Evolutionary Biology
Life Sciences
Marine environment
Methanol
Methanol - metabolism
Methylophaga
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Molecular Sequence Data
Nitrate Reductase
Nitrate Reductase - genetics
Nitrate Reductase - metabolism
Nitrates
Nitrates - metabolism
Nitrites
Nitrites - metabolism
original-article
Oxidation-Reduction
Oxygen
Oxygen - analysis
Phylogeny
Piscirickettsiaceae
Piscirickettsiaceae - classification
Piscirickettsiaceae - genetics
Piscirickettsiaceae - isolation & purification
Piscirickettsiaceae - metabolism
Reactors
RNA, Ribosomal, 16S
RNA, Ribosomal, 16S - genetics
Seawater
Seawater - chemistry
Seawater - microbiology
Sequence Analysis, DNA
Staining and Labeling
Staining and Labeling - methods
Taxa
Thiobacillus denitrificans
title Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T19%3A33%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dissimilatory%20reduction%20of%20nitrate%20in%20seawater%20by%20a%20Methylophaga%20strain%20containing%20two%20highly%20divergent%20narG%20sequences&rft.jtitle=The%20ISME%20Journal&rft.au=Auclair,%20Julie&rft.date=2010-10-01&rft.volume=4&rft.issue=10&rft.spage=1302&rft.epage=1313&rft.pages=1302-1313&rft.issn=1751-7362&rft.eissn=1751-7370&rft_id=info:doi/10.1038/ismej.2010.47&rft_dat=%3Cproquest_hal_p%3E755163680%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1029886103&rft_id=info:pmid/20393572&rfr_iscdi=true