Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test
Background: Rapid urease test (CLO‐test) is an inexpensive and quick method for diagnosis of Helicobacter pylori infection with controversial results in children. We evaluated the performance of CLO‐test in relation to endoscopic and histological findings in children with H. pylori infection. Mater...
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| Veröffentlicht in: | Helicobacter (Cambridge, Mass.) Mass.), 2010-06, Vol.15 (3), p.227-232 |
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| creator | Roma-Giannikou, Eleftheria Roubani, Alkaterini Sgouras, Dionyssios N. Panayiotou, Joanna Van-Vliet, Catherine Polyzos, Alexandros Roka, Kleoniki Daikos, George |
| description | Background: Rapid urease test (CLO‐test) is an inexpensive and quick method for diagnosis of Helicobacter pylori infection with controversial results in children. We evaluated the performance of CLO‐test in relation to endoscopic and histological findings in children with H. pylori infection.
Materials and methods: We studied the medical records of children with H. pylori infection who were diagnosed between 1989 and 2009. Noninfected children were used as controls. H. pylori infection was defined by positive culture or by two other positive tests (histology and CLO‐test, or urea breath test when a single test was positive). All children had histology together with CLO‐test. Tissue culture was performed whenever possible.
Results: Five hundred thirty infected children (10.4 ± 3.0 years) and 1060 controls (7.3 ± 4.4 years) were studied. Sensitivity of CLO‐test was 83.4% (95% CI, 79.9–86.3%), of culture 84.6% (95% CI, 78.7–89.1%), of histology 93.2% (95% CI, 90.7–95.1%), and specificity 99% (95% CI, 98.2–99.4%), 100%, and 100% respectively. CLO‐test positivity was correlated with higher bacterial density (p |
| doi_str_mv | 10.1111/j.1523-5378.2010.00756.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_pasteur_00734681v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>746302414</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4756-d3beb55836ee4ee3dc353cda4fac47ee837a1b20465f7c9f191365057894f7ef3</originalsourceid><addsrcrecordid>eNqNkV1v0zAUhiMEYmPwF5DvuEqxc-w4QdxMXVkmOpDGBpeW45xQlzTO7JS1_35uO8ol-MbW8XO-3jdJCKMTFs_75YSJDFIBsphkNEYplSKfbJ4lp8eP5_FNC0g5FOVJ8iqEJaVUAC9fJicZFUJCLk6TYdY3Lhg3WENuMYyBtM6TcYHkwuqfvQs2ENeSCjtrXK3NiJ4M2855S676Fs1oXU9sT6YL2zUe-w_ku-5so_fxmHijB9uQO4864L7B6-RFq7uAb57us-Tu0-x2WqXzr5dX0_N5anhcJW2gxlqIAnJEjgiNAQGm0bzVEUAsQGpWZ5TnopWmbFnJ4j5UyKLkrcQWzpL0UHehOzV4u9J-q5y2qjqfq0GHEddeRdmA5wX7zSL_7sAP3t2v46BqZYPBrtM9unVQkudAM874v0kAiPpyGsniQBrvQvDYHgdhVO2MVEu180vt_FI7I9XeSLWJqW-fmqzrFTbHxD_OReDjAXiwHW7_u7CqZvP4-CuPjUpsjuna_1K5BCnUjy-XqiquL77Jz9fqBh4BKU268A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733305540</pqid></control><display><type>article</type><title>Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Roma-Giannikou, Eleftheria ; Roubani, Alkaterini ; Sgouras, Dionyssios N. ; Panayiotou, Joanna ; Van-Vliet, Catherine ; Polyzos, Alexandros ; Roka, Kleoniki ; Daikos, George</creator><creatorcontrib>Roma-Giannikou, Eleftheria ; Roubani, Alkaterini ; Sgouras, Dionyssios N. ; Panayiotou, Joanna ; Van-Vliet, Catherine ; Polyzos, Alexandros ; Roka, Kleoniki ; Daikos, George</creatorcontrib><description>Background: Rapid urease test (CLO‐test) is an inexpensive and quick method for diagnosis of Helicobacter pylori infection with controversial results in children. We evaluated the performance of CLO‐test in relation to endoscopic and histological findings in children with H. pylori infection.
Materials and methods: We studied the medical records of children with H. pylori infection who were diagnosed between 1989 and 2009. Noninfected children were used as controls. H. pylori infection was defined by positive culture or by two other positive tests (histology and CLO‐test, or urea breath test when a single test was positive). All children had histology together with CLO‐test. Tissue culture was performed whenever possible.
Results: Five hundred thirty infected children (10.4 ± 3.0 years) and 1060 controls (7.3 ± 4.4 years) were studied. Sensitivity of CLO‐test was 83.4% (95% CI, 79.9–86.3%), of culture 84.6% (95% CI, 78.7–89.1%), of histology 93.2% (95% CI, 90.7–95.1%), and specificity 99% (95% CI, 98.2–99.4%), 100%, and 100% respectively. CLO‐test positivity was correlated with higher bacterial density (p < .001), activity (p < .001) and severity of gastritis (p < .01), older age (p < .01), and the presence of antral nodularity (p < .001). When CLO‐test was positive, the concordance with histology and culture was high (95.5 and 89.2% respectively), whereas low concordance was observed when CLO‐test was negative (17.05 and 45.83% respectively).
Conclusions: CLO‐test had lower sensitivity and comparable specificity with histology. Both tests should be performed concurrently to accurately diagnose H. pylori infection in children.</description><identifier>ISSN: 1083-4389</identifier><identifier>EISSN: 1523-5378</identifier><identifier>DOI: 10.1111/j.1523-5378.2010.00756.x</identifier><identifier>PMID: 20557365</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Bacterial density ; Breath Tests ; Child ; Child, Preschool ; Children ; Endoscopy, Gastrointestinal ; Female ; Gastritis ; Geriatrics ; H. pylori ; Helicobacter Infections ; Helicobacter Infections - diagnosis ; Helicobacter Infections - microbiology ; Helicobacter Infections - pathology ; Helicobacter pylori ; Helicobacter pylori - enzymology ; Helicobacter pylori - isolation & purification ; Helicobacter pylori - metabolism ; Histocytochemistry ; Humans ; Infection ; Life Sciences ; Male ; medical records ; Microbiology and Parasitology ; nodularity ; rapid urease test ; Sensitivity and Specificity ; Tissue culture ; Urea ; Urease ; Urease - analysis ; validation</subject><ispartof>Helicobacter (Cambridge, Mass.), 2010-06, Vol.15 (3), p.227-232</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4756-d3beb55836ee4ee3dc353cda4fac47ee837a1b20465f7c9f191365057894f7ef3</citedby><cites>FETCH-LOGICAL-c4756-d3beb55836ee4ee3dc353cda4fac47ee837a1b20465f7c9f191365057894f7ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1523-5378.2010.00756.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1523-5378.2010.00756.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20557365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://riip.hal.science/pasteur-00734681$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Roma-Giannikou, Eleftheria</creatorcontrib><creatorcontrib>Roubani, Alkaterini</creatorcontrib><creatorcontrib>Sgouras, Dionyssios N.</creatorcontrib><creatorcontrib>Panayiotou, Joanna</creatorcontrib><creatorcontrib>Van-Vliet, Catherine</creatorcontrib><creatorcontrib>Polyzos, Alexandros</creatorcontrib><creatorcontrib>Roka, Kleoniki</creatorcontrib><creatorcontrib>Daikos, George</creatorcontrib><title>Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test</title><title>Helicobacter (Cambridge, Mass.)</title><addtitle>Helicobacter</addtitle><description>Background: Rapid urease test (CLO‐test) is an inexpensive and quick method for diagnosis of Helicobacter pylori infection with controversial results in children. We evaluated the performance of CLO‐test in relation to endoscopic and histological findings in children with H. pylori infection.
Materials and methods: We studied the medical records of children with H. pylori infection who were diagnosed between 1989 and 2009. Noninfected children were used as controls. H. pylori infection was defined by positive culture or by two other positive tests (histology and CLO‐test, or urea breath test when a single test was positive). All children had histology together with CLO‐test. Tissue culture was performed whenever possible.
Results: Five hundred thirty infected children (10.4 ± 3.0 years) and 1060 controls (7.3 ± 4.4 years) were studied. Sensitivity of CLO‐test was 83.4% (95% CI, 79.9–86.3%), of culture 84.6% (95% CI, 78.7–89.1%), of histology 93.2% (95% CI, 90.7–95.1%), and specificity 99% (95% CI, 98.2–99.4%), 100%, and 100% respectively. CLO‐test positivity was correlated with higher bacterial density (p < .001), activity (p < .001) and severity of gastritis (p < .01), older age (p < .01), and the presence of antral nodularity (p < .001). When CLO‐test was positive, the concordance with histology and culture was high (95.5 and 89.2% respectively), whereas low concordance was observed when CLO‐test was negative (17.05 and 45.83% respectively).
Conclusions: CLO‐test had lower sensitivity and comparable specificity with histology. Both tests should be performed concurrently to accurately diagnose H. pylori infection in children.</description><subject>Adolescent</subject><subject>Bacterial density</subject><subject>Breath Tests</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Endoscopy, Gastrointestinal</subject><subject>Female</subject><subject>Gastritis</subject><subject>Geriatrics</subject><subject>H. pylori</subject><subject>Helicobacter Infections</subject><subject>Helicobacter Infections - diagnosis</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter Infections - pathology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - enzymology</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Helicobacter pylori - metabolism</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Infection</subject><subject>Life Sciences</subject><subject>Male</subject><subject>medical records</subject><subject>Microbiology and Parasitology</subject><subject>nodularity</subject><subject>rapid urease test</subject><subject>Sensitivity and Specificity</subject><subject>Tissue culture</subject><subject>Urea</subject><subject>Urease</subject><subject>Urease - analysis</subject><subject>validation</subject><issn>1083-4389</issn><issn>1523-5378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1v0zAUhiMEYmPwF5DvuEqxc-w4QdxMXVkmOpDGBpeW45xQlzTO7JS1_35uO8ol-MbW8XO-3jdJCKMTFs_75YSJDFIBsphkNEYplSKfbJ4lp8eP5_FNC0g5FOVJ8iqEJaVUAC9fJicZFUJCLk6TYdY3Lhg3WENuMYyBtM6TcYHkwuqfvQs2ENeSCjtrXK3NiJ4M2855S676Fs1oXU9sT6YL2zUe-w_ku-5so_fxmHijB9uQO4864L7B6-RFq7uAb57us-Tu0-x2WqXzr5dX0_N5anhcJW2gxlqIAnJEjgiNAQGm0bzVEUAsQGpWZ5TnopWmbFnJ4j5UyKLkrcQWzpL0UHehOzV4u9J-q5y2qjqfq0GHEddeRdmA5wX7zSL_7sAP3t2v46BqZYPBrtM9unVQkudAM874v0kAiPpyGsniQBrvQvDYHgdhVO2MVEu180vt_FI7I9XeSLWJqW-fmqzrFTbHxD_OReDjAXiwHW7_u7CqZvP4-CuPjUpsjuna_1K5BCnUjy-XqiquL77Jz9fqBh4BKU268A</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>Roma-Giannikou, Eleftheria</creator><creator>Roubani, Alkaterini</creator><creator>Sgouras, Dionyssios N.</creator><creator>Panayiotou, Joanna</creator><creator>Van-Vliet, Catherine</creator><creator>Polyzos, Alexandros</creator><creator>Roka, Kleoniki</creator><creator>Daikos, George</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>1XC</scope></search><sort><creationdate>201006</creationdate><title>Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test</title><author>Roma-Giannikou, Eleftheria ; Roubani, Alkaterini ; Sgouras, Dionyssios N. ; Panayiotou, Joanna ; Van-Vliet, Catherine ; Polyzos, Alexandros ; Roka, Kleoniki ; Daikos, George</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4756-d3beb55836ee4ee3dc353cda4fac47ee837a1b20465f7c9f191365057894f7ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Bacterial density</topic><topic>Breath Tests</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Endoscopy, Gastrointestinal</topic><topic>Female</topic><topic>Gastritis</topic><topic>Geriatrics</topic><topic>H. pylori</topic><topic>Helicobacter Infections</topic><topic>Helicobacter Infections - diagnosis</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter Infections - pathology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - enzymology</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Helicobacter pylori - metabolism</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Infection</topic><topic>Life Sciences</topic><topic>Male</topic><topic>medical records</topic><topic>Microbiology and Parasitology</topic><topic>nodularity</topic><topic>rapid urease test</topic><topic>Sensitivity and Specificity</topic><topic>Tissue culture</topic><topic>Urea</topic><topic>Urease</topic><topic>Urease - analysis</topic><topic>validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roma-Giannikou, Eleftheria</creatorcontrib><creatorcontrib>Roubani, Alkaterini</creatorcontrib><creatorcontrib>Sgouras, Dionyssios N.</creatorcontrib><creatorcontrib>Panayiotou, Joanna</creatorcontrib><creatorcontrib>Van-Vliet, Catherine</creatorcontrib><creatorcontrib>Polyzos, Alexandros</creatorcontrib><creatorcontrib>Roka, Kleoniki</creatorcontrib><creatorcontrib>Daikos, George</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Helicobacter (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roma-Giannikou, Eleftheria</au><au>Roubani, Alkaterini</au><au>Sgouras, Dionyssios N.</au><au>Panayiotou, Joanna</au><au>Van-Vliet, Catherine</au><au>Polyzos, Alexandros</au><au>Roka, Kleoniki</au><au>Daikos, George</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test</atitle><jtitle>Helicobacter (Cambridge, Mass.)</jtitle><addtitle>Helicobacter</addtitle><date>2010-06</date><risdate>2010</risdate><volume>15</volume><issue>3</issue><spage>227</spage><epage>232</epage><pages>227-232</pages><issn>1083-4389</issn><eissn>1523-5378</eissn><abstract>Background: Rapid urease test (CLO‐test) is an inexpensive and quick method for diagnosis of Helicobacter pylori infection with controversial results in children. We evaluated the performance of CLO‐test in relation to endoscopic and histological findings in children with H. pylori infection.
Materials and methods: We studied the medical records of children with H. pylori infection who were diagnosed between 1989 and 2009. Noninfected children were used as controls. H. pylori infection was defined by positive culture or by two other positive tests (histology and CLO‐test, or urea breath test when a single test was positive). All children had histology together with CLO‐test. Tissue culture was performed whenever possible.
Results: Five hundred thirty infected children (10.4 ± 3.0 years) and 1060 controls (7.3 ± 4.4 years) were studied. Sensitivity of CLO‐test was 83.4% (95% CI, 79.9–86.3%), of culture 84.6% (95% CI, 78.7–89.1%), of histology 93.2% (95% CI, 90.7–95.1%), and specificity 99% (95% CI, 98.2–99.4%), 100%, and 100% respectively. CLO‐test positivity was correlated with higher bacterial density (p < .001), activity (p < .001) and severity of gastritis (p < .01), older age (p < .01), and the presence of antral nodularity (p < .001). When CLO‐test was positive, the concordance with histology and culture was high (95.5 and 89.2% respectively), whereas low concordance was observed when CLO‐test was negative (17.05 and 45.83% respectively).
Conclusions: CLO‐test had lower sensitivity and comparable specificity with histology. Both tests should be performed concurrently to accurately diagnose H. pylori infection in children.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20557365</pmid><doi>10.1111/j.1523-5378.2010.00756.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Helicobacter (Cambridge, Mass.), 2010-06, Vol.15 (3), p.227-232 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescent Bacterial density Breath Tests Child Child, Preschool Children Endoscopy, Gastrointestinal Female Gastritis Geriatrics H. pylori Helicobacter Infections Helicobacter Infections - diagnosis Helicobacter Infections - microbiology Helicobacter Infections - pathology Helicobacter pylori Helicobacter pylori - enzymology Helicobacter pylori - isolation & purification Helicobacter pylori - metabolism Histocytochemistry Humans Infection Life Sciences Male medical records Microbiology and Parasitology nodularity rapid urease test Sensitivity and Specificity Tissue culture Urea Urease Urease - analysis validation |
| title | Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test |
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