Dengue-3 Outbreak in Paraguay: Investigations Using Capillary Blood Samples on Filter Paper

During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the tec...

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Veröffentlicht in:The American journal of tropical medicine and hygiene 2008-11, Vol.79 (5), p.685-687
Hauptverfasser: Matheus, Severine, Meynard, Jean-Baptiste, Lavergne, Anne, Girod, Romain, Moua, David, Labeau, Bhety, Dussart, Philippe, Lacoste, Vincent, Deparis, Xavier
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container_title The American journal of tropical medicine and hygiene
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creator Matheus, Severine
Meynard, Jean-Baptiste
Lavergne, Anne
Girod, Romain
Moua, David
Labeau, Bhety
Dussart, Philippe
Lacoste, Vincent
Deparis, Xavier
description During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the techniques used. Molecular analyses detected the dengue-3 serotype in 22 patients and additionally the dengue-2 serotype in two patients. Therefore two different serotypes were co-circulating during this outbreak. Overall, this study validates the use of dried-blood samples for field screening investigations. Indeed, all types of laboratory studies of dengue were possible with samples consisting of a few drops of dried blood from finger pricks.
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subjects Antigens, Viral
Antigens, Viral - blood
Dengue
Dengue - blood
Dengue - epidemiology
Dengue Virus
Dengue Virus - classification
Dengue Virus - genetics
Dengue Virus - immunology
Dengue Virus - isolation & purification
Dengue virus type 2
Disease Outbreaks
Enzyme-Linked Immunosorbent Assay
Humans
Life Sciences
Microbiology and Parasitology
Paraguay
Paraguay - epidemiology
Reverse Transcriptase Polymerase Chain Reaction
Virology
title Dengue-3 Outbreak in Paraguay: Investigations Using Capillary Blood Samples on Filter Paper
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