GNAI3, GNAT2, AMPD2, GSTM are clustered in 120 kb of Chinese hamster chromosome 1q
We studied a polygenic region located on Chromosome (Chr) 1q in Chinese hamster cells that is coamplified along with the AMPD2 gene. Previous sequence analysis identified both members of the GSTM family and the GNAI3 gene within a cloned 120-kb region surrounding the AMPD2 locus. We show here that t...
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| Veröffentlicht in: | Mammalian genome 1996-06, Vol.7 (6), p.429-432 |
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| creator | Baron, B Fernandez, M A Carignon, S Toledo, F Buttin, G Debatisse, M |
| description | We studied a polygenic region located on Chromosome (Chr) 1q in Chinese hamster cells that is coamplified along with the AMPD2 gene. Previous sequence analysis identified both members of the GSTM family and the GNAI3 gene within a cloned 120-kb region surrounding the AMPD2 locus. We show here that the GNAT2 gene, which is inactive in the fibroblastic cells, lies within the 20 kb separating the transcriptionally active GNAI3 and AMPD2 genes. We map most gene ends by sequence comparison with human homologs; one is inferred from the presence of an unmethylated CpG island. This Chinese hamster locus corresponds to a region of conserved linkage between human Chr 1 (locus 1p13) and mouse Chr 3 (position 52.5 cM), where Gnai-3 and Gnat-2 have been mapped. The AMPD2 gene is presently unlocalized in human genome; its proposed position on mouse Chr 3 is at 53.4 cM. Our results, obtained by physical mapping, strongly suggest that the order and possibly the tight linkage of these genes are conserved on all three genomes. |
| doi_str_mv | 10.1007/s003359900127 |
| format | Article |
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Previous sequence analysis identified both members of the GSTM family and the GNAI3 gene within a cloned 120-kb region surrounding the AMPD2 locus. We show here that the GNAT2 gene, which is inactive in the fibroblastic cells, lies within the 20 kb separating the transcriptionally active GNAI3 and AMPD2 genes. We map most gene ends by sequence comparison with human homologs; one is inferred from the presence of an unmethylated CpG island. This Chinese hamster locus corresponds to a region of conserved linkage between human Chr 1 (locus 1p13) and mouse Chr 3 (position 52.5 cM), where Gnai-3 and Gnat-2 have been mapped. The AMPD2 gene is presently unlocalized in human genome; its proposed position on mouse Chr 3 is at 53.4 cM. Our results, obtained by physical mapping, strongly suggest that the order and possibly the tight linkage of these genes are conserved on all three genomes.</description><identifier>ISSN: 0938-8990</identifier><identifier>EISSN: 1432-1777</identifier><identifier>DOI: 10.1007/s003359900127</identifier><identifier>PMID: 8662225</identifier><language>eng</language><publisher>United States: Springer Verlag</publisher><subject>Animals ; Base Sequence ; Blotting, Southern ; Cell Line ; Chromosome Mapping ; Cricetinae ; Cricetulus - genetics ; DNA, Complementary ; Genes - genetics ; Genetic Linkage ; GTP-Binding Proteins - chemistry ; GTP-Binding Proteins - genetics ; Life Sciences ; Protein Structure, Tertiary ; RNA, Messenger - genetics ; Sequence Homology, Nucleic Acid</subject><ispartof>Mammalian genome, 1996-06, Vol.7 (6), p.429-432</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-530585b533ed3b12c3e826759eaf713e5fbe2e04ba53ae01a09c09c3bd5a8b0e3</citedby><cites>FETCH-LOGICAL-c356t-530585b533ed3b12c3e826759eaf713e5fbe2e04ba53ae01a09c09c3bd5a8b0e3</cites><orcidid>0000-0002-7113-3965</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8662225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-03199029$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Baron, B</creatorcontrib><creatorcontrib>Fernandez, M A</creatorcontrib><creatorcontrib>Carignon, S</creatorcontrib><creatorcontrib>Toledo, F</creatorcontrib><creatorcontrib>Buttin, G</creatorcontrib><creatorcontrib>Debatisse, M</creatorcontrib><title>GNAI3, GNAT2, AMPD2, GSTM are clustered in 120 kb of Chinese hamster chromosome 1q</title><title>Mammalian genome</title><addtitle>Mamm Genome</addtitle><description>We studied a polygenic region located on Chromosome (Chr) 1q in Chinese hamster cells that is coamplified along with the AMPD2 gene. Previous sequence analysis identified both members of the GSTM family and the GNAI3 gene within a cloned 120-kb region surrounding the AMPD2 locus. We show here that the GNAT2 gene, which is inactive in the fibroblastic cells, lies within the 20 kb separating the transcriptionally active GNAI3 and AMPD2 genes. We map most gene ends by sequence comparison with human homologs; one is inferred from the presence of an unmethylated CpG island. This Chinese hamster locus corresponds to a region of conserved linkage between human Chr 1 (locus 1p13) and mouse Chr 3 (position 52.5 cM), where Gnai-3 and Gnat-2 have been mapped. The AMPD2 gene is presently unlocalized in human genome; its proposed position on mouse Chr 3 is at 53.4 cM. Our results, obtained by physical mapping, strongly suggest that the order and possibly the tight linkage of these genes are conserved on all three genomes.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>Cell Line</subject><subject>Chromosome Mapping</subject><subject>Cricetinae</subject><subject>Cricetulus - genetics</subject><subject>DNA, Complementary</subject><subject>Genes - genetics</subject><subject>Genetic Linkage</subject><subject>GTP-Binding Proteins - chemistry</subject><subject>GTP-Binding Proteins - genetics</subject><subject>Life Sciences</subject><subject>Protein Structure, Tertiary</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0938-8990</issn><issn>1432-1777</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1LAzEQBuAgSq0fR49CTp66Osk0m-yxVG0LrYrWc8huZ-nqbleTVvDfm9IieBIC72EehgkvYxcCrgWAvgkAiCrLAITUB6wr-igTobU-ZF3I0CQmzo7ZSQhvkehU6A7rmDSVUqouex49DCbY4zHmsscHs6fbGKOX-Yw7T7yoN2FNnha8WnEhgb_nvC35cFmtKBBfumY75sXSt00b2oa4-DxjR6WrA53v85S93t_Nh-Nk-jiaDAfTpECVrhOFoIzKFSItMBeyQDIy1SojV2qBpMqcJEE_dwodgXCQFfFhvlDO5EB4ynq7vUtX2w9fNc5_29ZVdjyY2moVyDcWUMTfy-xLRH614x--_dxQWNumCgXVtVtRuwlWG-wbaeS_UKgUdGYgwmQHC9-G4Kn8vUKA3XZj_3QT_eV-8SZvaPGr92XgD_3KhAE</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Baron, B</creator><creator>Fernandez, M A</creator><creator>Carignon, S</creator><creator>Toledo, F</creator><creator>Buttin, G</creator><creator>Debatisse, M</creator><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-7113-3965</orcidid></search><sort><creationdate>19960601</creationdate><title>GNAI3, GNAT2, AMPD2, GSTM are clustered in 120 kb of Chinese hamster chromosome 1q</title><author>Baron, B ; Fernandez, M A ; Carignon, S ; Toledo, F ; Buttin, G ; Debatisse, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-530585b533ed3b12c3e826759eaf713e5fbe2e04ba53ae01a09c09c3bd5a8b0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>Cell Line</topic><topic>Chromosome Mapping</topic><topic>Cricetinae</topic><topic>Cricetulus - genetics</topic><topic>DNA, Complementary</topic><topic>Genes - genetics</topic><topic>Genetic Linkage</topic><topic>GTP-Binding Proteins - chemistry</topic><topic>GTP-Binding Proteins - genetics</topic><topic>Life Sciences</topic><topic>Protein Structure, Tertiary</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baron, B</creatorcontrib><creatorcontrib>Fernandez, M A</creatorcontrib><creatorcontrib>Carignon, S</creatorcontrib><creatorcontrib>Toledo, F</creatorcontrib><creatorcontrib>Buttin, G</creatorcontrib><creatorcontrib>Debatisse, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Mammalian genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baron, B</au><au>Fernandez, M A</au><au>Carignon, S</au><au>Toledo, F</au><au>Buttin, G</au><au>Debatisse, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GNAI3, GNAT2, AMPD2, GSTM are clustered in 120 kb of Chinese hamster chromosome 1q</atitle><jtitle>Mammalian genome</jtitle><addtitle>Mamm Genome</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>7</volume><issue>6</issue><spage>429</spage><epage>432</epage><pages>429-432</pages><issn>0938-8990</issn><eissn>1432-1777</eissn><abstract>We studied a polygenic region located on Chromosome (Chr) 1q in Chinese hamster cells that is coamplified along with the AMPD2 gene. Previous sequence analysis identified both members of the GSTM family and the GNAI3 gene within a cloned 120-kb region surrounding the AMPD2 locus. We show here that the GNAT2 gene, which is inactive in the fibroblastic cells, lies within the 20 kb separating the transcriptionally active GNAI3 and AMPD2 genes. We map most gene ends by sequence comparison with human homologs; one is inferred from the presence of an unmethylated CpG island. This Chinese hamster locus corresponds to a region of conserved linkage between human Chr 1 (locus 1p13) and mouse Chr 3 (position 52.5 cM), where Gnai-3 and Gnat-2 have been mapped. The AMPD2 gene is presently unlocalized in human genome; its proposed position on mouse Chr 3 is at 53.4 cM. Our results, obtained by physical mapping, strongly suggest that the order and possibly the tight linkage of these genes are conserved on all three genomes.</abstract><cop>United States</cop><pub>Springer Verlag</pub><pmid>8662225</pmid><doi>10.1007/s003359900127</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-7113-3965</orcidid></addata></record> |
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| ispartof | Mammalian genome, 1996-06, Vol.7 (6), p.429-432 |
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| subjects | Animals Base Sequence Blotting, Southern Cell Line Chromosome Mapping Cricetinae Cricetulus - genetics DNA, Complementary Genes - genetics Genetic Linkage GTP-Binding Proteins - chemistry GTP-Binding Proteins - genetics Life Sciences Protein Structure, Tertiary RNA, Messenger - genetics Sequence Homology, Nucleic Acid |
| title | GNAI3, GNAT2, AMPD2, GSTM are clustered in 120 kb of Chinese hamster chromosome 1q |
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