Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration
Peripheral nerve regeneration is regulated through the coordinated spatio‐temporal activation of multiple cellular pathways. In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 day...
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| Veröffentlicht in: | Journal of cellular physiology 2018-08, Vol.233 (8), p.6207-6223 |
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| creator | Vergara, Daniele Romano, Alessandro Stanca, Eleonora La Pesa, Velia Aloisi, Laura De Domenico, Stefania Franck, Julien Cicalini, Ilaria Giudetti, Anna Storelli, Elisa Pieragostino, Damiana Fournier, Isabelle Sannino, Alessandro Salzet, Michel Cerri, Federica Quattrini, Angelo Maffia, Michele |
| description | Peripheral nerve regeneration is regulated through the coordinated spatio‐temporal activation of multiple cellular pathways. In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography mass‐spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. These include proteins involved in cytoskeleton signaling and remodeling, acute phase response, and cellular metabolism. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand. In particular, we identified a group of proteins involved in lipid uptake and lipid storage metabolism. Immunofluorescent staining for acyl‐CoA diacylglycerol acyltransferase 1 (DGAT1) and DAGT2 expression provided evidence for the expression and localization of these two isoforms in Schwann cells at the injury site in the sciatic nerve. This further supports a specific local regulation of lipid metabolism in peripheral nerve after damage.
In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography‐mass spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand. |
| doi_str_mv | 10.1002/jcp.26478 |
| format | Article |
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In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography‐mass spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.26478</identifier><identifier>PMID: 29327509</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Acyltransferase ; Bioinformatics ; Biological activity ; Cytoskeleton ; DGAT ; Diglycerides ; Injuries ; Isoforms ; LC‐MS/MS ; Life Sciences ; Lipid metabolism ; Lipids ; Liquid chromatography ; Localization ; Metabolism ; peripheral nerve injury ; Peripheral nerves ; Proteins ; Proteomics ; Regeneration ; Schwann cells ; Sciatic nerve ; Spectrometry</subject><ispartof>Journal of cellular physiology, 2018-08, Vol.233 (8), p.6207-6223</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3908-9f2f16bc23e966d29ae984ab0b4762395fc618b9e6f4c44f4a87a9cb6fee3fd23</citedby><cites>FETCH-LOGICAL-c3908-9f2f16bc23e966d29ae984ab0b4762395fc618b9e6f4c44f4a87a9cb6fee3fd23</cites><orcidid>0000-0002-2396-7674 ; 0000-0003-1096-5044</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.26478$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.26478$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29327509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-02940755$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Vergara, Daniele</creatorcontrib><creatorcontrib>Romano, Alessandro</creatorcontrib><creatorcontrib>Stanca, Eleonora</creatorcontrib><creatorcontrib>La Pesa, Velia</creatorcontrib><creatorcontrib>Aloisi, Laura</creatorcontrib><creatorcontrib>De Domenico, Stefania</creatorcontrib><creatorcontrib>Franck, Julien</creatorcontrib><creatorcontrib>Cicalini, Ilaria</creatorcontrib><creatorcontrib>Giudetti, Anna</creatorcontrib><creatorcontrib>Storelli, Elisa</creatorcontrib><creatorcontrib>Pieragostino, Damiana</creatorcontrib><creatorcontrib>Fournier, Isabelle</creatorcontrib><creatorcontrib>Sannino, Alessandro</creatorcontrib><creatorcontrib>Salzet, Michel</creatorcontrib><creatorcontrib>Cerri, Federica</creatorcontrib><creatorcontrib>Quattrini, Angelo</creatorcontrib><creatorcontrib>Maffia, Michele</creatorcontrib><title>Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Peripheral nerve regeneration is regulated through the coordinated spatio‐temporal activation of multiple cellular pathways. In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography mass‐spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. These include proteins involved in cytoskeleton signaling and remodeling, acute phase response, and cellular metabolism. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand. In particular, we identified a group of proteins involved in lipid uptake and lipid storage metabolism. Immunofluorescent staining for acyl‐CoA diacylglycerol acyltransferase 1 (DGAT1) and DAGT2 expression provided evidence for the expression and localization of these two isoforms in Schwann cells at the injury site in the sciatic nerve. This further supports a specific local regulation of lipid metabolism in peripheral nerve after damage.
In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography‐mass spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand.</description><subject>Acyltransferase</subject><subject>Bioinformatics</subject><subject>Biological activity</subject><subject>Cytoskeleton</subject><subject>DGAT</subject><subject>Diglycerides</subject><subject>Injuries</subject><subject>Isoforms</subject><subject>LC‐MS/MS</subject><subject>Life Sciences</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Localization</subject><subject>Metabolism</subject><subject>peripheral nerve injury</subject><subject>Peripheral nerves</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Regeneration</subject><subject>Schwann cells</subject><subject>Sciatic nerve</subject><subject>Spectrometry</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kcFu1DAURS0EosPAgh9AkdgUibS24zjxshpBCxqJLmBtOc5zx0MSBzuZod_AT_e1KV0gsbKle3Ts9y4hbxk9Y5Ty870dz7gUVf2MrBhVVS5kyZ-TFWYsV6VgJ-RVSntKqVJF8ZKccFXwqqRqRf5cxzBB6L3N4PcYISUfhmyMwfkOsuAyP-znCG0WzZSNEP24g2i6bIB4gJRFOIDpMG586MKNtw_RdAzxZ8rM0N6bLEoRNSkF682E8NFPu8WAghvAm5nw2dfkhTNdgjeP55r8-Pzp--Yq3367_LK52Oa2ULTOleOOycbyApSULVcGVC1MQxtRSV6o0lnJ6kaBdMIK4YSpK6NsIx1A4VperMnHxbsznR6j70281cF4fXWx1X5IEHtNuRK0KssDQ_x0wXGWXzOkSfc-Weg6M0CYk2aqVpLKGle7Ju__QfdhjgMOozkaZVlVrETqw0LZGFKK4J4-wai-L1RjofqhUGTfPRrnpof2ifzbIALnC3DEwm7_b9JfN9eL8g4qca1L</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Vergara, Daniele</creator><creator>Romano, Alessandro</creator><creator>Stanca, Eleonora</creator><creator>La Pesa, Velia</creator><creator>Aloisi, Laura</creator><creator>De Domenico, Stefania</creator><creator>Franck, Julien</creator><creator>Cicalini, Ilaria</creator><creator>Giudetti, Anna</creator><creator>Storelli, Elisa</creator><creator>Pieragostino, Damiana</creator><creator>Fournier, Isabelle</creator><creator>Sannino, Alessandro</creator><creator>Salzet, Michel</creator><creator>Cerri, Federica</creator><creator>Quattrini, Angelo</creator><creator>Maffia, Michele</creator><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-2396-7674</orcidid><orcidid>https://orcid.org/0000-0003-1096-5044</orcidid></search><sort><creationdate>201808</creationdate><title>Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration</title><author>Vergara, Daniele ; 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In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography mass‐spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. These include proteins involved in cytoskeleton signaling and remodeling, acute phase response, and cellular metabolism. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand. In particular, we identified a group of proteins involved in lipid uptake and lipid storage metabolism. Immunofluorescent staining for acyl‐CoA diacylglycerol acyltransferase 1 (DGAT1) and DAGT2 expression provided evidence for the expression and localization of these two isoforms in Schwann cells at the injury site in the sciatic nerve. This further supports a specific local regulation of lipid metabolism in peripheral nerve after damage.
In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography‐mass spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29327509</pmid><doi>10.1002/jcp.26478</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-2396-7674</orcidid><orcidid>https://orcid.org/0000-0003-1096-5044</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acyltransferase Bioinformatics Biological activity Cytoskeleton DGAT Diglycerides Injuries Isoforms LC‐MS/MS Life Sciences Lipid metabolism Lipids Liquid chromatography Localization Metabolism peripheral nerve injury Peripheral nerves Proteins Proteomics Regeneration Schwann cells Sciatic nerve Spectrometry |
| title | Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration |
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