In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics
Aim To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta‐percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material. Methodology A collagen/alginate composite scaffold wa...
Gespeichert in:
| Veröffentlicht in: | International endodontic journal 2017-01, Vol.50 (1), p.48-57 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 57 |
|---|---|
| container_issue | 1 |
| container_start_page | 48 |
| container_title | International endodontic journal |
| container_volume | 50 |
| creator | Devillard, R. Rémy, M. Kalisky, J. Bourget, J.‐M. Kérourédan, O. Siadous, R. Bareille, R. Amédée‐Vilamitjana, J. Chassande, O. Fricain, J.‐C. |
| description | Aim
To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta‐percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
Methodology
A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta‐percha point‐like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t‐tests and the Mann–Whitney U test were performed.
Results
The fabrication method developed enabled the shape of a gutta‐percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P |
| doi_str_mv | 10.1111/iej.12591 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_inserm_02870711v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1920588790</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3901-623279ebf0ec7557cf90c9209512a72bee3d6d9d6863a57e7478751f793b37473</originalsourceid><addsrcrecordid>eNp1kU1rGzEQhkVoSFy3h_yBstBLC9lYH9ZKOoaQ1g6GXNJLL0LWjhyZXcmV1i7-91WyaQ6BzGU-eHiZmRehC4KvSImZh-0VoVyREzQhrOH1U_0BTTCZs5pKyc_Rx5y3GGOOGTlD57RpOBaUTdDvZagOfkixMjlDzj2EoYquMpWNXWc2EGam2_hgBiiTfhezL1W2xrnYtZWLqUpQKEhm8AeoILSxjWHwNn9Cp850GT6_5Cn69eP24WZRr-5_Lm-uV7VlCpO6oYwKBWuHwQrOhXUKW0Wx4oQaQdcArG1a1TayYYYLEHMhBSdOKLZmpWFTdDnqPppO75LvTTrqaLxeXK-0DxlSrzGVAgtCDqTg30Z8l-KfPeRB9z5bKMcGiPusiSzfmXPJm4J-fYNu4z6FcowmZUMupVC4UN9HyqaYcwL3ugTB-skfXfzRz_4U9suL4n7dQ_tK_jekALMR-Os7OL6vpJe3d6PkP9frl_8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1920588790</pqid></control><display><type>article</type><title>In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Devillard, R. ; Rémy, M. ; Kalisky, J. ; Bourget, J.‐M. ; Kérourédan, O. ; Siadous, R. ; Bareille, R. ; Amédée‐Vilamitjana, J. ; Chassande, O. ; Fricain, J.‐C.</creator><creatorcontrib>Devillard, R. ; Rémy, M. ; Kalisky, J. ; Bourget, J.‐M. ; Kérourédan, O. ; Siadous, R. ; Bareille, R. ; Amédée‐Vilamitjana, J. ; Chassande, O. ; Fricain, J.‐C.</creatorcontrib><description>Aim
To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta‐percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
Methodology
A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta‐percha point‐like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t‐tests and the Mann–Whitney U test were performed.
Results
The fabrication method developed enabled the shape of a gutta‐percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).
Conclusions
The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.12591</identifier><identifier>PMID: 26650723</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>adult stem cells ; Alginates - pharmacology ; Alginic acid ; Cell culture ; cell differentiation ; Cell Differentiation - drug effects ; Collagen ; Collagen - pharmacology ; Compaction ; Contraction ; Dentistry ; Endodontics ; Extracellular matrix ; Gelation ; Glucuronic Acid - pharmacology ; Gutta-Percha ; Hexuronic Acids - pharmacology ; Humans ; hydrogel ; Life Sciences ; Mechanical properties ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - cytology ; Regeneration ; regenerative medicine ; Root canals ; Sodium alginate ; Stem cells ; Tissue culture ; tissue engineering ; Tissue Scaffolds - chemistry</subject><ispartof>International endodontic journal, 2017-01, Vol.50 (1), p.48-57</ispartof><rights>2015 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3901-623279ebf0ec7557cf90c9209512a72bee3d6d9d6863a57e7478751f793b37473</citedby><cites>FETCH-LOGICAL-c3901-623279ebf0ec7557cf90c9209512a72bee3d6d9d6863a57e7478751f793b37473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.12591$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.12591$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26650723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-02870711$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Devillard, R.</creatorcontrib><creatorcontrib>Rémy, M.</creatorcontrib><creatorcontrib>Kalisky, J.</creatorcontrib><creatorcontrib>Bourget, J.‐M.</creatorcontrib><creatorcontrib>Kérourédan, O.</creatorcontrib><creatorcontrib>Siadous, R.</creatorcontrib><creatorcontrib>Bareille, R.</creatorcontrib><creatorcontrib>Amédée‐Vilamitjana, J.</creatorcontrib><creatorcontrib>Chassande, O.</creatorcontrib><creatorcontrib>Fricain, J.‐C.</creatorcontrib><title>In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta‐percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
Methodology
A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta‐percha point‐like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t‐tests and the Mann–Whitney U test were performed.
Results
The fabrication method developed enabled the shape of a gutta‐percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).
Conclusions
The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.</description><subject>adult stem cells</subject><subject>Alginates - pharmacology</subject><subject>Alginic acid</subject><subject>Cell culture</subject><subject>cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Collagen</subject><subject>Collagen - pharmacology</subject><subject>Compaction</subject><subject>Contraction</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Extracellular matrix</subject><subject>Gelation</subject><subject>Glucuronic Acid - pharmacology</subject><subject>Gutta-Percha</subject><subject>Hexuronic Acids - pharmacology</subject><subject>Humans</subject><subject>hydrogel</subject><subject>Life Sciences</subject><subject>Mechanical properties</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchyme</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Regeneration</subject><subject>regenerative medicine</subject><subject>Root canals</subject><subject>Sodium alginate</subject><subject>Stem cells</subject><subject>Tissue culture</subject><subject>tissue engineering</subject><subject>Tissue Scaffolds - chemistry</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1rGzEQhkVoSFy3h_yBstBLC9lYH9ZKOoaQ1g6GXNJLL0LWjhyZXcmV1i7-91WyaQ6BzGU-eHiZmRehC4KvSImZh-0VoVyREzQhrOH1U_0BTTCZs5pKyc_Rx5y3GGOOGTlD57RpOBaUTdDvZagOfkixMjlDzj2EoYquMpWNXWc2EGam2_hgBiiTfhezL1W2xrnYtZWLqUpQKEhm8AeoILSxjWHwNn9Cp850GT6_5Cn69eP24WZRr-5_Lm-uV7VlCpO6oYwKBWuHwQrOhXUKW0Wx4oQaQdcArG1a1TayYYYLEHMhBSdOKLZmpWFTdDnqPppO75LvTTrqaLxeXK-0DxlSrzGVAgtCDqTg30Z8l-KfPeRB9z5bKMcGiPusiSzfmXPJm4J-fYNu4z6FcowmZUMupVC4UN9HyqaYcwL3ugTB-skfXfzRz_4U9suL4n7dQ_tK_jekALMR-Os7OL6vpJe3d6PkP9frl_8</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Devillard, R.</creator><creator>Rémy, M.</creator><creator>Kalisky, J.</creator><creator>Bourget, J.‐M.</creator><creator>Kérourédan, O.</creator><creator>Siadous, R.</creator><creator>Bareille, R.</creator><creator>Amédée‐Vilamitjana, J.</creator><creator>Chassande, O.</creator><creator>Fricain, J.‐C.</creator><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>201701</creationdate><title>In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics</title><author>Devillard, R. ; Rémy, M. ; Kalisky, J. ; Bourget, J.‐M. ; Kérourédan, O. ; Siadous, R. ; Bareille, R. ; Amédée‐Vilamitjana, J. ; Chassande, O. ; Fricain, J.‐C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3901-623279ebf0ec7557cf90c9209512a72bee3d6d9d6863a57e7478751f793b37473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>adult stem cells</topic><topic>Alginates - pharmacology</topic><topic>Alginic acid</topic><topic>Cell culture</topic><topic>cell differentiation</topic><topic>Cell Differentiation - drug effects</topic><topic>Collagen</topic><topic>Collagen - pharmacology</topic><topic>Compaction</topic><topic>Contraction</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Extracellular matrix</topic><topic>Gelation</topic><topic>Glucuronic Acid - pharmacology</topic><topic>Gutta-Percha</topic><topic>Hexuronic Acids - pharmacology</topic><topic>Humans</topic><topic>hydrogel</topic><topic>Life Sciences</topic><topic>Mechanical properties</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchyme</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Regeneration</topic><topic>regenerative medicine</topic><topic>Root canals</topic><topic>Sodium alginate</topic><topic>Stem cells</topic><topic>Tissue culture</topic><topic>tissue engineering</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Devillard, R.</creatorcontrib><creatorcontrib>Rémy, M.</creatorcontrib><creatorcontrib>Kalisky, J.</creatorcontrib><creatorcontrib>Bourget, J.‐M.</creatorcontrib><creatorcontrib>Kérourédan, O.</creatorcontrib><creatorcontrib>Siadous, R.</creatorcontrib><creatorcontrib>Bareille, R.</creatorcontrib><creatorcontrib>Amédée‐Vilamitjana, J.</creatorcontrib><creatorcontrib>Chassande, O.</creatorcontrib><creatorcontrib>Fricain, J.‐C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Devillard, R.</au><au>Rémy, M.</au><au>Kalisky, J.</au><au>Bourget, J.‐M.</au><au>Kérourédan, O.</au><au>Siadous, R.</au><au>Bareille, R.</au><au>Amédée‐Vilamitjana, J.</au><au>Chassande, O.</au><au>Fricain, J.‐C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2017-01</date><risdate>2017</risdate><volume>50</volume><issue>1</issue><spage>48</spage><epage>57</epage><pages>48-57</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta‐percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
Methodology
A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta‐percha point‐like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t‐tests and the Mann–Whitney U test were performed.
Results
The fabrication method developed enabled the shape of a gutta‐percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).
Conclusions
The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>26650723</pmid><doi>10.1111/iej.12591</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0143-2885 |
| ispartof | International endodontic journal, 2017-01, Vol.50 (1), p.48-57 |
| issn | 0143-2885 1365-2591 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_inserm_02870711v1 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | adult stem cells Alginates - pharmacology Alginic acid Cell culture cell differentiation Cell Differentiation - drug effects Collagen Collagen - pharmacology Compaction Contraction Dentistry Endodontics Extracellular matrix Gelation Glucuronic Acid - pharmacology Gutta-Percha Hexuronic Acids - pharmacology Humans hydrogel Life Sciences Mechanical properties Mesenchymal Stromal Cells - cytology Mesenchyme Osteoblastogenesis Osteoblasts Osteoblasts - cytology Regeneration regenerative medicine Root canals Sodium alginate Stem cells Tissue culture tissue engineering Tissue Scaffolds - chemistry |
| title | In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T00%3A18%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20assessment%20of%20a%20collagen/alginate%20composite%20scaffold%20for%20regenerative%20endodontics&rft.jtitle=International%20endodontic%20journal&rft.au=Devillard,%20R.&rft.date=2017-01&rft.volume=50&rft.issue=1&rft.spage=48&rft.epage=57&rft.pages=48-57&rft.issn=0143-2885&rft.eissn=1365-2591&rft_id=info:doi/10.1111/iej.12591&rft_dat=%3Cproquest_hal_p%3E1920588790%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1920588790&rft_id=info:pmid/26650723&rfr_iscdi=true |