Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage
The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na...
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Veröffentlicht in: | Molecular and cellular biochemistry 1997-04, Vol.169 (1-2), p.21-25 |
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creator | Ferri, C Evans, R L Paulais, M Tanimura, A Turner, R J |
description | The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes. |
doi_str_mv | 10.1023/A:1006878324913 |
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It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1023/A:1006878324913</identifier><identifier>PMID: 9089627</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Ammonium Chloride - pharmacology ; Animals ; Calcium - metabolism ; Carbachol - pharmacology ; Carrier Proteins - metabolism ; Cell Size ; Cells ; Cellular Biology ; Chlorides - metabolism ; Kinases ; Life Sciences ; Membrane Proteins - metabolism ; Muscarinic Agonists - pharmacology ; Parotid Gland - cytology ; Parotid Gland - drug effects ; Potassium - metabolism ; Protein Kinase Inhibitors ; Proteins ; Rats ; Rats, Wistar ; Sodium - metabolism ; Sodium-Potassium-Chloride Symporters ; Staurosporine - pharmacology ; Subcellular Processes ; Up-Regulation</subject><ispartof>Molecular and cellular biochemistry, 1997-04, Vol.169 (1-2), p.21-25</ispartof><rights>Kluwer Academic Publishers 1997</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-4895-2110</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9089627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-02448201$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferri, C</creatorcontrib><creatorcontrib>Evans, R L</creatorcontrib><creatorcontrib>Paulais, M</creatorcontrib><creatorcontrib>Tanimura, A</creatorcontrib><creatorcontrib>Turner, R J</creatorcontrib><title>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.</description><subject>Ammonium Chloride - pharmacology</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Carbachol - pharmacology</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Size</subject><subject>Cells</subject><subject>Cellular Biology</subject><subject>Chlorides - metabolism</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Membrane Proteins - metabolism</subject><subject>Muscarinic Agonists - pharmacology</subject><subject>Parotid Gland - cytology</subject><subject>Parotid Gland - drug effects</subject><subject>Potassium - metabolism</subject><subject>Protein Kinase Inhibitors</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium - metabolism</subject><subject>Sodium-Potassium-Chloride Symporters</subject><subject>Staurosporine - pharmacology</subject><subject>Subcellular Processes</subject><subject>Up-Regulation</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0c1u1DAQAGALgcpSOHNCsjggKgiMfxI73FarQhEruMA58tqTxiVrp7ZTad-Ax25WXXHoxWONPs1oZgh5zeATAy4-r78wgEYrLbhsmXhCVqxWolr-7VOyAgFQaabUc_Ii5xuABTN2Rs5a0G3D1Yr8u7zzDoNFWgZT6DxVCa_n0RQfA439kkU6mRSLd_Snef_hovpxfPhmrKiNJZmQp5gKJro70OEwYSoxeEvzkHz4a66R-kxDLNTN0-itKeiO0uf4yL0kz3ozZnx1iufkz9fL35uravvr2_fNelsNHHipdMut5brnwikLwnKuuTC67VmNwjU7a6VEWUvTO1lrBw3WtVXKNLKvVS8acU4-PtQdzNhNye9NOnTR-O5qve18yJj2HXApNQd2xxb-7oFPKd7OmEu399niOJqAcc6d0svWQckFvn0Eb-KcwjJKp-qGaQH8WO3NCc27Pbr__U_nEPd0Hotv</recordid><startdate>199704</startdate><enddate>199704</enddate><creator>Ferri, C</creator><creator>Evans, R L</creator><creator>Paulais, M</creator><creator>Tanimura, A</creator><creator>Turner, R J</creator><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4895-2110</orcidid></search><sort><creationdate>199704</creationdate><title>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</title><author>Ferri, C ; Evans, R L ; Paulais, M ; Tanimura, A ; Turner, R J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h202t-892cc28f23d7c03c22823a89f15e3d6bcc44e454afd458d06e55c77a64f57f363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Ammonium Chloride - 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferri, C</au><au>Evans, R L</au><au>Paulais, M</au><au>Tanimura, A</au><au>Turner, R J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1997-04</date><risdate>1997</risdate><volume>169</volume><issue>1-2</issue><spage>21</spage><epage>25</epage><pages>21-25</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>9089627</pmid><doi>10.1023/A:1006878324913</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-4895-2110</orcidid></addata></record> |
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subjects | Ammonium Chloride - pharmacology Animals Calcium - metabolism Carbachol - pharmacology Carrier Proteins - metabolism Cell Size Cells Cellular Biology Chlorides - metabolism Kinases Life Sciences Membrane Proteins - metabolism Muscarinic Agonists - pharmacology Parotid Gland - cytology Parotid Gland - drug effects Potassium - metabolism Protein Kinase Inhibitors Proteins Rats Rats, Wistar Sodium - metabolism Sodium-Potassium-Chloride Symporters Staurosporine - pharmacology Subcellular Processes Up-Regulation |
title | Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage |
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