Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage

The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular and cellular biochemistry 1997-04, Vol.169 (1-2), p.21-25
Hauptverfasser: Ferri, C, Evans, R L, Paulais, M, Tanimura, A, Turner, R J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 25
container_issue 1-2
container_start_page 21
container_title Molecular and cellular biochemistry
container_volume 169
creator Ferri, C
Evans, R L
Paulais, M
Tanimura, A
Turner, R J
description The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.
doi_str_mv 10.1023/A:1006878324913
format Article
fullrecord <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_inserm_02448201v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78913074</sourcerecordid><originalsourceid>FETCH-LOGICAL-h202t-892cc28f23d7c03c22823a89f15e3d6bcc44e454afd458d06e55c77a64f57f363</originalsourceid><addsrcrecordid>eNpd0c1u1DAQAGALgcpSOHNCsjggKgiMfxI73FarQhEruMA58tqTxiVrp7ZTad-Ax25WXXHoxWONPs1oZgh5zeATAy4-r78wgEYrLbhsmXhCVqxWolr-7VOyAgFQaabUc_Ii5xuABTN2Rs5a0G3D1Yr8u7zzDoNFWgZT6DxVCa_n0RQfA439kkU6mRSLd_Snef_hovpxfPhmrKiNJZmQp5gKJro70OEwYSoxeEvzkHz4a66R-kxDLNTN0-itKeiO0uf4yL0kz3ozZnx1iufkz9fL35uravvr2_fNelsNHHipdMut5brnwikLwnKuuTC67VmNwjU7a6VEWUvTO1lrBw3WtVXKNLKvVS8acU4-PtQdzNhNye9NOnTR-O5qve18yJj2HXApNQd2xxb-7oFPKd7OmEu399niOJqAcc6d0svWQckFvn0Eb-KcwjJKp-qGaQH8WO3NCc27Pbr__U_nEPd0Hotv</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>756183021</pqid></control><display><type>article</type><title>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Ferri, C ; Evans, R L ; Paulais, M ; Tanimura, A ; Turner, R J</creator><creatorcontrib>Ferri, C ; Evans, R L ; Paulais, M ; Tanimura, A ; Turner, R J</creatorcontrib><description>The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1023/A:1006878324913</identifier><identifier>PMID: 9089627</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Ammonium Chloride - pharmacology ; Animals ; Calcium - metabolism ; Carbachol - pharmacology ; Carrier Proteins - metabolism ; Cell Size ; Cells ; Cellular Biology ; Chlorides - metabolism ; Kinases ; Life Sciences ; Membrane Proteins - metabolism ; Muscarinic Agonists - pharmacology ; Parotid Gland - cytology ; Parotid Gland - drug effects ; Potassium - metabolism ; Protein Kinase Inhibitors ; Proteins ; Rats ; Rats, Wistar ; Sodium - metabolism ; Sodium-Potassium-Chloride Symporters ; Staurosporine - pharmacology ; Subcellular Processes ; Up-Regulation</subject><ispartof>Molecular and cellular biochemistry, 1997-04, Vol.169 (1-2), p.21-25</ispartof><rights>Kluwer Academic Publishers 1997</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-4895-2110</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9089627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-02448201$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferri, C</creatorcontrib><creatorcontrib>Evans, R L</creatorcontrib><creatorcontrib>Paulais, M</creatorcontrib><creatorcontrib>Tanimura, A</creatorcontrib><creatorcontrib>Turner, R J</creatorcontrib><title>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.</description><subject>Ammonium Chloride - pharmacology</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Carbachol - pharmacology</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Size</subject><subject>Cells</subject><subject>Cellular Biology</subject><subject>Chlorides - metabolism</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Membrane Proteins - metabolism</subject><subject>Muscarinic Agonists - pharmacology</subject><subject>Parotid Gland - cytology</subject><subject>Parotid Gland - drug effects</subject><subject>Potassium - metabolism</subject><subject>Protein Kinase Inhibitors</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium - metabolism</subject><subject>Sodium-Potassium-Chloride Symporters</subject><subject>Staurosporine - pharmacology</subject><subject>Subcellular Processes</subject><subject>Up-Regulation</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0c1u1DAQAGALgcpSOHNCsjggKgiMfxI73FarQhEruMA58tqTxiVrp7ZTad-Ax25WXXHoxWONPs1oZgh5zeATAy4-r78wgEYrLbhsmXhCVqxWolr-7VOyAgFQaabUc_Ii5xuABTN2Rs5a0G3D1Yr8u7zzDoNFWgZT6DxVCa_n0RQfA439kkU6mRSLd_Snef_hovpxfPhmrKiNJZmQp5gKJro70OEwYSoxeEvzkHz4a66R-kxDLNTN0-itKeiO0uf4yL0kz3ozZnx1iufkz9fL35uravvr2_fNelsNHHipdMut5brnwikLwnKuuTC67VmNwjU7a6VEWUvTO1lrBw3WtVXKNLKvVS8acU4-PtQdzNhNye9NOnTR-O5qve18yJj2HXApNQd2xxb-7oFPKd7OmEu399niOJqAcc6d0svWQckFvn0Eb-KcwjJKp-qGaQH8WO3NCc27Pbr__U_nEPd0Hotv</recordid><startdate>199704</startdate><enddate>199704</enddate><creator>Ferri, C</creator><creator>Evans, R L</creator><creator>Paulais, M</creator><creator>Tanimura, A</creator><creator>Turner, R J</creator><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4895-2110</orcidid></search><sort><creationdate>199704</creationdate><title>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</title><author>Ferri, C ; Evans, R L ; Paulais, M ; Tanimura, A ; Turner, R J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h202t-892cc28f23d7c03c22823a89f15e3d6bcc44e454afd458d06e55c77a64f57f363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Ammonium Chloride - pharmacology</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Carbachol - pharmacology</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Size</topic><topic>Cells</topic><topic>Cellular Biology</topic><topic>Chlorides - metabolism</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Membrane Proteins - metabolism</topic><topic>Muscarinic Agonists - pharmacology</topic><topic>Parotid Gland - cytology</topic><topic>Parotid Gland - drug effects</topic><topic>Potassium - metabolism</topic><topic>Protein Kinase Inhibitors</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sodium - metabolism</topic><topic>Sodium-Potassium-Chloride Symporters</topic><topic>Staurosporine - pharmacology</topic><topic>Subcellular Processes</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferri, C</creatorcontrib><creatorcontrib>Evans, R L</creatorcontrib><creatorcontrib>Paulais, M</creatorcontrib><creatorcontrib>Tanimura, A</creatorcontrib><creatorcontrib>Turner, R J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferri, C</au><au>Evans, R L</au><au>Paulais, M</au><au>Tanimura, A</au><au>Turner, R J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1997-04</date><risdate>1997</risdate><volume>169</volume><issue>1-2</issue><spage>21</spage><epage>25</epage><pages>21-25</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>The application of Ca2+ mobilizing secretagogues to rat parotid acini results in a significant decrease in cell volume (15-30%) due to isotonic salt loss. It is often assumed that the effects of such an isotonic volume decrease can be mimicked by anisotonic cell shrinkage. We demonstrate that the Na(+)-K(+)-2Cl- cotransporter in these cells is up-regulated by Ca2+ mobilizing secretagogues as well as by cell shrinkage in hypertonic media. However, we find that although the protein kinase inhibitors staurosporine (0.3 microM) and K252a (0.6 microM) significantly blunt the latter up-regulation, they are without effect on the former. These observations suggest that hypertonic and isotonic shrinkage do not result in the activation of the same intracellular signalling pathways, and indicate that anisotonic volume perturbations may not provide good experimental models of physiologic isotonic volume changes.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>9089627</pmid><doi>10.1023/A:1006878324913</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-4895-2110</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0300-8177
ispartof Molecular and cellular biochemistry, 1997-04, Vol.169 (1-2), p.21-25
issn 0300-8177
1573-4919
language eng
recordid cdi_hal_primary_oai_HAL_inserm_02448201v1
source MEDLINE; Springer Nature - Complete Springer Journals
subjects Ammonium Chloride - pharmacology
Animals
Calcium - metabolism
Carbachol - pharmacology
Carrier Proteins - metabolism
Cell Size
Cells
Cellular Biology
Chlorides - metabolism
Kinases
Life Sciences
Membrane Proteins - metabolism
Muscarinic Agonists - pharmacology
Parotid Gland - cytology
Parotid Gland - drug effects
Potassium - metabolism
Protein Kinase Inhibitors
Proteins
Rats
Rats, Wistar
Sodium - metabolism
Sodium-Potassium-Chloride Symporters
Staurosporine - pharmacology
Subcellular Processes
Up-Regulation
title Evidence that up-regulation of the parotid Na(+)-K(+)-2Cl- cotransporter by hypertonic shrinkage is not duplicated by isotonic shrinkage
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T03%3A23%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evidence%20that%20up-regulation%20of%20the%20parotid%20Na(+)-K(+)-2Cl-%20cotransporter%20by%20hypertonic%20shrinkage%20is%20not%20duplicated%20by%20isotonic%20shrinkage&rft.jtitle=Molecular%20and%20cellular%20biochemistry&rft.au=Ferri,%20C&rft.date=1997-04&rft.volume=169&rft.issue=1-2&rft.spage=21&rft.epage=25&rft.pages=21-25&rft.issn=0300-8177&rft.eissn=1573-4919&rft_id=info:doi/10.1023/A:1006878324913&rft_dat=%3Cproquest_hal_p%3E78913074%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=756183021&rft_id=info:pmid/9089627&rfr_iscdi=true