Gene targeting in rats using transcription activator-like effector nucleases
The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important fo...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2014-08, Vol.69 (1), p.102-107 |
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| creator | Ménoret, Séverine Tesson, Laurent Rémy, Séverine Usal, Claire Thépenier, Virginie Thinard, Reynald Ouisse, Laure-Hélène De Cian, Anne Giovannangeli, Carine Concordet, Jean-Paul Anegon, Ignacio |
| description | The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models. |
| doi_str_mv | 10.1016/j.ymeth.2014.02.027 |
| format | Article |
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Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.</description><identifier>ISSN: 1046-2023</identifier><identifier>EISSN: 1095-9130</identifier><identifier>DOI: 10.1016/j.ymeth.2014.02.027</identifier><identifier>PMID: 24583114</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Deoxyribonucleases - chemistry ; Deoxyribonucleases - genetics ; DNA End-Joining Repair ; Embryo Transfer ; Embryo, Mammalian ; Female ; Gene editing ; Gene Knockout Techniques ; Homologous Recombination ; Human health and pathology ; Knockout ; Life Sciences ; Microinjections ; Mutagenesis, Site-Directed - methods ; Non-homologous end joining ; Rat model ; Rats ; Rats, Sprague-Dawley ; TALE nucleases</subject><ispartof>Methods (San Diego, Calif.), 2014-08, Vol.69 (1), p.102-107</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-456b5262a83285334d6640d6a62eccef5c30b81bca09f46051414d70dcc31e643</citedby><cites>FETCH-LOGICAL-c532t-456b5262a83285334d6640d6a62eccef5c30b81bca09f46051414d70dcc31e643</cites><orcidid>0000-0002-1737-2543 ; 0000-0002-5241-880X ; 0000-0001-8924-4316 ; 0000-0001-8700-5645 ; 0000-0002-7715-8864</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1046202314000826$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24583114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-02164327$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ménoret, Séverine</creatorcontrib><creatorcontrib>Tesson, Laurent</creatorcontrib><creatorcontrib>Rémy, Séverine</creatorcontrib><creatorcontrib>Usal, Claire</creatorcontrib><creatorcontrib>Thépenier, Virginie</creatorcontrib><creatorcontrib>Thinard, Reynald</creatorcontrib><creatorcontrib>Ouisse, Laure-Hélène</creatorcontrib><creatorcontrib>De Cian, Anne</creatorcontrib><creatorcontrib>Giovannangeli, Carine</creatorcontrib><creatorcontrib>Concordet, Jean-Paul</creatorcontrib><creatorcontrib>Anegon, Ignacio</creatorcontrib><title>Gene targeting in rats using transcription activator-like effector nucleases</title><title>Methods (San Diego, Calif.)</title><addtitle>Methods</addtitle><description>The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. 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| ispartof | Methods (San Diego, Calif.), 2014-08, Vol.69 (1), p.102-107 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Deoxyribonucleases - chemistry Deoxyribonucleases - genetics DNA End-Joining Repair Embryo Transfer Embryo, Mammalian Female Gene editing Gene Knockout Techniques Homologous Recombination Human health and pathology Knockout Life Sciences Microinjections Mutagenesis, Site-Directed - methods Non-homologous end joining Rat model Rats Rats, Sprague-Dawley TALE nucleases |
| title | Gene targeting in rats using transcription activator-like effector nucleases |
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