Two means of transcriptional reactivation within heterochromatin
Summary DNA methylation levels and specific histone modifications of chromatin in interphase nuclei are taken as an indicator of transcriptional activity or silencing. Arabidopsis mutants impaired in maintenance of transcriptional gene silencing (TGS) alleviate TGS with or without affecting DNA meth...
Gespeichert in:
| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2003-02, Vol.33 (4), p.743-749 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 749 |
|---|---|
| container_issue | 4 |
| container_start_page | 743 |
| container_title | The Plant journal : for cell and molecular biology |
| container_volume | 33 |
| creator | Probst, Aline V. Fransz, Paul F. Paszkowski, Jerzy Scheid, Ortrun Mittelsten |
| description | Summary
DNA methylation levels and specific histone modifications of chromatin in interphase nuclei are taken as an indicator of transcriptional activity or silencing. Arabidopsis mutants impaired in maintenance of transcriptional gene silencing (TGS) alleviate TGS with or without affecting DNA methylation. Mutant ddm1, representing the first type, lacks a chromatin remodeling factor that regulates histone and DNA methylation. Mutant mom1, representing the second type, is affected in a different but still unknown silencing mechanism. Both classes of mutation have been studied mainly for their effects on specific loci. Here, we describe the cytological analysis of chromatin in ddm1 and mom1 mutants. The ddm1 mutation causes a striking decondensation of centromeric heterochromatin, a re‐distribution of the remaining methylation of DNA, and a drastic change in the pattern of histone modification. A complex transgenic locus, which underwent stable inactivation and became heterochromatin‐like, follows similar structural alterations. In contrast, nuclear organization in mom1 appears unaltered, demonstrating an involvement of MOM1 in transcriptional regulation within a heterochromatic environment. |
| doi_str_mv | 10.1046/j.1365-313X.2003.01667.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_inserm_01629519v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18708263</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5297-bec1c1c2b7c184b1167eb168965186bd7c348216cea75c9ea661e79e71d566253</originalsourceid><addsrcrecordid>eNqNkU2P0zAQhi0EYsvCX0C5wIkEj52M4wMSqxWwoEpwKBI3y3GnqqskLna63f33OLTaPYJ88Iz9zIfel7ECeAW8xve7CiQ2pQT5qxKcy4oDoqrunrDFw8dTtuAaealqEBfsRUo7zkFJrJ-zCxDIdW60YB9Xx1AMZMdUhE0xxRy46PeTD6Pti0jWTf7Wzmlx9NPWj8WWJorBbWMY8vv4kj3b2D7Rq_N9yX5-_rS6vimX3798vb5alq4RWpUdOchHdMpBW3cAqKgDbDU20GK3Vk7WrQB0ZFXjNFlEIKVJwbpBFI28ZO9Ofbe2N_voBxvvTbDe3FwtjR8TxcFkFYRuQN9Cxt-e8H0Mvw-UJjP45Kjv7UjhkIySXOXp_wahVbwVKDPYnkAXQ0qRNg9bADezK2ZnZvHNLL6ZXTF_XTF3ufT1ecahG2j9WHi2IQNvzoBNzvabbIPz6ZGrG6VB6cx9OHFH39P9fy9gVj--zZH8A-EDp7A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18708263</pqid></control><display><type>article</type><title>Two means of transcriptional reactivation within heterochromatin</title><source>Wiley Free Content</source><source>MEDLINE</source><source>IngentaConnect Free/Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Probst, Aline V. ; Fransz, Paul F. ; Paszkowski, Jerzy ; Scheid, Ortrun Mittelsten</creator><creatorcontrib>Probst, Aline V. ; Fransz, Paul F. ; Paszkowski, Jerzy ; Scheid, Ortrun Mittelsten</creatorcontrib><description>Summary
DNA methylation levels and specific histone modifications of chromatin in interphase nuclei are taken as an indicator of transcriptional activity or silencing. Arabidopsis mutants impaired in maintenance of transcriptional gene silencing (TGS) alleviate TGS with or without affecting DNA methylation. Mutant ddm1, representing the first type, lacks a chromatin remodeling factor that regulates histone and DNA methylation. Mutant mom1, representing the second type, is affected in a different but still unknown silencing mechanism. Both classes of mutation have been studied mainly for their effects on specific loci. Here, we describe the cytological analysis of chromatin in ddm1 and mom1 mutants. The ddm1 mutation causes a striking decondensation of centromeric heterochromatin, a re‐distribution of the remaining methylation of DNA, and a drastic change in the pattern of histone modification. A complex transgenic locus, which underwent stable inactivation and became heterochromatin‐like, follows similar structural alterations. In contrast, nuclear organization in mom1 appears unaltered, demonstrating an involvement of MOM1 in transcriptional regulation within a heterochromatic environment.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1046/j.1365-313X.2003.01667.x</identifier><identifier>PMID: 12609046</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins ; Biological and medical sciences ; Cell Nucleus - genetics ; Cell Nucleus - physiology ; ddm1 ; DNA Methylation ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; gene silencing ; Gene Silencing - physiology ; heterochromatin ; Heterochromatin - genetics ; Heterochromatin - metabolism ; histone modifications ; Histones - metabolism ; In Situ Hybridization, Fluorescence ; Life Sciences ; Molecular and cellular biology ; Molecular genetics ; mom1 ; Mutation ; nuclear organization ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription. Transcription factor. Splicing. Rna processing ; Transcriptional Activation - genetics ; Transcriptional Activation - physiology</subject><ispartof>The Plant journal : for cell and molecular biology, 2003-02, Vol.33 (4), p.743-749</ispartof><rights>2003 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5297-bec1c1c2b7c184b1167eb168965186bd7c348216cea75c9ea661e79e71d566253</citedby><cites>FETCH-LOGICAL-c5297-bec1c1c2b7c184b1167eb168965186bd7c348216cea75c9ea661e79e71d566253</cites><orcidid>0000-0001-9534-8058</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-313X.2003.01667.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-313X.2003.01667.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,1427,27903,27904,45553,45554,46387,46811</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14579179$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12609046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-01629519$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Probst, Aline V.</creatorcontrib><creatorcontrib>Fransz, Paul F.</creatorcontrib><creatorcontrib>Paszkowski, Jerzy</creatorcontrib><creatorcontrib>Scheid, Ortrun Mittelsten</creatorcontrib><title>Two means of transcriptional reactivation within heterochromatin</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
DNA methylation levels and specific histone modifications of chromatin in interphase nuclei are taken as an indicator of transcriptional activity or silencing. Arabidopsis mutants impaired in maintenance of transcriptional gene silencing (TGS) alleviate TGS with or without affecting DNA methylation. Mutant ddm1, representing the first type, lacks a chromatin remodeling factor that regulates histone and DNA methylation. Mutant mom1, representing the second type, is affected in a different but still unknown silencing mechanism. Both classes of mutation have been studied mainly for their effects on specific loci. Here, we describe the cytological analysis of chromatin in ddm1 and mom1 mutants. The ddm1 mutation causes a striking decondensation of centromeric heterochromatin, a re‐distribution of the remaining methylation of DNA, and a drastic change in the pattern of histone modification. A complex transgenic locus, which underwent stable inactivation and became heterochromatin‐like, follows similar structural alterations. In contrast, nuclear organization in mom1 appears unaltered, demonstrating an involvement of MOM1 in transcriptional regulation within a heterochromatic environment.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - physiology</subject><subject>ddm1</subject><subject>DNA Methylation</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene silencing</subject><subject>Gene Silencing - physiology</subject><subject>heterochromatin</subject><subject>Heterochromatin - genetics</subject><subject>Heterochromatin - metabolism</subject><subject>histone modifications</subject><subject>Histones - metabolism</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Life Sciences</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>mom1</subject><subject>Mutation</subject><subject>nuclear organization</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Transcriptional Activation - genetics</subject><subject>Transcriptional Activation - physiology</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P0zAQhi0EYsvCX0C5wIkEj52M4wMSqxWwoEpwKBI3y3GnqqskLna63f33OLTaPYJ88Iz9zIfel7ECeAW8xve7CiQ2pQT5qxKcy4oDoqrunrDFw8dTtuAaealqEBfsRUo7zkFJrJ-zCxDIdW60YB9Xx1AMZMdUhE0xxRy46PeTD6Pti0jWTf7Wzmlx9NPWj8WWJorBbWMY8vv4kj3b2D7Rq_N9yX5-_rS6vimX3798vb5alq4RWpUdOchHdMpBW3cAqKgDbDU20GK3Vk7WrQB0ZFXjNFlEIKVJwbpBFI28ZO9Ofbe2N_voBxvvTbDe3FwtjR8TxcFkFYRuQN9Cxt-e8H0Mvw-UJjP45Kjv7UjhkIySXOXp_wahVbwVKDPYnkAXQ0qRNg9bADezK2ZnZvHNLL6ZXTF_XTF3ufT1ecahG2j9WHi2IQNvzoBNzvabbIPz6ZGrG6VB6cx9OHFH39P9fy9gVj--zZH8A-EDp7A</recordid><startdate>200302</startdate><enddate>200302</enddate><creator>Probst, Aline V.</creator><creator>Fransz, Paul F.</creator><creator>Paszkowski, Jerzy</creator><creator>Scheid, Ortrun Mittelsten</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-9534-8058</orcidid></search><sort><creationdate>200302</creationdate><title>Two means of transcriptional reactivation within heterochromatin</title><author>Probst, Aline V. ; Fransz, Paul F. ; Paszkowski, Jerzy ; Scheid, Ortrun Mittelsten</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5297-bec1c1c2b7c184b1167eb168965186bd7c348216cea75c9ea661e79e71d566253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - physiology</topic><topic>ddm1</topic><topic>DNA Methylation</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene silencing</topic><topic>Gene Silencing - physiology</topic><topic>heterochromatin</topic><topic>Heterochromatin - genetics</topic><topic>Heterochromatin - metabolism</topic><topic>histone modifications</topic><topic>Histones - metabolism</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Life Sciences</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>mom1</topic><topic>Mutation</topic><topic>nuclear organization</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transcriptional Activation - genetics</topic><topic>Transcriptional Activation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Probst, Aline V.</creatorcontrib><creatorcontrib>Fransz, Paul F.</creatorcontrib><creatorcontrib>Paszkowski, Jerzy</creatorcontrib><creatorcontrib>Scheid, Ortrun Mittelsten</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Probst, Aline V.</au><au>Fransz, Paul F.</au><au>Paszkowski, Jerzy</au><au>Scheid, Ortrun Mittelsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two means of transcriptional reactivation within heterochromatin</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2003-02</date><risdate>2003</risdate><volume>33</volume><issue>4</issue><spage>743</spage><epage>749</epage><pages>743-749</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
DNA methylation levels and specific histone modifications of chromatin in interphase nuclei are taken as an indicator of transcriptional activity or silencing. Arabidopsis mutants impaired in maintenance of transcriptional gene silencing (TGS) alleviate TGS with or without affecting DNA methylation. Mutant ddm1, representing the first type, lacks a chromatin remodeling factor that regulates histone and DNA methylation. Mutant mom1, representing the second type, is affected in a different but still unknown silencing mechanism. Both classes of mutation have been studied mainly for their effects on specific loci. Here, we describe the cytological analysis of chromatin in ddm1 and mom1 mutants. The ddm1 mutation causes a striking decondensation of centromeric heterochromatin, a re‐distribution of the remaining methylation of DNA, and a drastic change in the pattern of histone modification. A complex transgenic locus, which underwent stable inactivation and became heterochromatin‐like, follows similar structural alterations. In contrast, nuclear organization in mom1 appears unaltered, demonstrating an involvement of MOM1 in transcriptional regulation within a heterochromatic environment.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>12609046</pmid><doi>10.1046/j.1365-313X.2003.01667.x</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9534-8058</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-7412 |
| ispartof | The Plant journal : for cell and molecular biology, 2003-02, Vol.33 (4), p.743-749 |
| issn | 0960-7412 1365-313X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_inserm_01629519v1 |
| source | Wiley Free Content; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins Biological and medical sciences Cell Nucleus - genetics Cell Nucleus - physiology ddm1 DNA Methylation DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology gene silencing Gene Silencing - physiology heterochromatin Heterochromatin - genetics Heterochromatin - metabolism histone modifications Histones - metabolism In Situ Hybridization, Fluorescence Life Sciences Molecular and cellular biology Molecular genetics mom1 Mutation nuclear organization Transcription Factors - genetics Transcription Factors - metabolism Transcription. Transcription factor. Splicing. Rna processing Transcriptional Activation - genetics Transcriptional Activation - physiology |
| title | Two means of transcriptional reactivation within heterochromatin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T07%3A02%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Two%20means%20of%20transcriptional%20reactivation%20within%20heterochromatin&rft.jtitle=The%20Plant%20journal%20:%20for%20cell%20and%20molecular%20biology&rft.au=Probst,%20Aline%20V.&rft.date=2003-02&rft.volume=33&rft.issue=4&rft.spage=743&rft.epage=749&rft.pages=743-749&rft.issn=0960-7412&rft.eissn=1365-313X&rft_id=info:doi/10.1046/j.1365-313X.2003.01667.x&rft_dat=%3Cproquest_hal_p%3E18708263%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18708263&rft_id=info:pmid/12609046&rfr_iscdi=true |