The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability
Scope Vitamin E is present in feed and food mainly as d‐α‐tocopherol (d‐α‐TOL) but also as all‐rac‐α‐tocopheryl acetate (rac‐α‐TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion....
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| Veröffentlicht in: | Molecular nutrition & food research 2013-07, Vol.57 (7), p.1237-1245 |
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| creator | Desmarchelier, Charles Tourniaire, Franck Prévéraud, Damien P. Samson-Kremser, Coralie Crenon, Isabelle Rosilio, Véronique Borel, Patrick |
| description | Scope
Vitamin E is present in feed and food mainly as d‐α‐tocopherol (d‐α‐TOL) but also as all‐rac‐α‐tocopheryl acetate (rac‐α‐TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion.
Methods and results
Using an in vitro digestion model, we showed that TAC was distributed between mixed micelles (36%), liposomes (9%), and nonsolubilized food debris (52%). A significant fraction of TAC was also found in emulsions when fat hydrolysis was not complete. Among the candidate esterases tested, i.e. cholesteryl ester hydrolase, pancreatic lipase, and pancreatic lipase‐related protein 2, only cholesteryl ester hydrolase was able to hydrolyze TAC to all‐rac‐α‐TOL, about five times more efficiently when it was incorporated into mixed micelles or liposomes than into emulsions or in the food matrix. Caco‐2 cells were able to hydrolyze TAC and to uptake TOL when TAC was incorporated into mixed micelles but not into emulsions.
Conclusion
During digestion, most TAC is recovered in matrices where its hydrolysis and its uptake by intestinal cells are markedly less efficient than in mixed micelles. |
| doi_str_mv | 10.1002/mnfr.201200720 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_inserm_01478557v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>MNFR1963</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4123-3bc71d41e627b4e9f467fe1e7873111f9491f7091b8c0b77930bd10c597004893</originalsourceid><addsrcrecordid>eNqFkU9v0zAchiMEYmNw5Yh84UaKHSdxfZyqdZvUDQRDHC3H-Xk1OHFlO13z5fhsOMtWjpz873le23qz7D3BC4Jx8bnrtV8UmBQYswK_yE5JTWheEkpfHudFdZK9CeEXxpQUJX2dnRS0Sg6np9mfuy2g1oToTTNE43ok-xZ5sDKaPaDt2Hpnx2ACchpFp9xuC360SCqIMgIyPYqPCVqDhz6iTqYoBQEpB4eUa_r7Z8gOHfSPOWkROmltOokwMSlh8BPamvtpI71DucG2CA47K5NvYkDWPaDGOLmXxsrGWBPHt9krLW2Ad0_jWfZjfXG3uso3Xy6vV-ebXKXv05w2ipG2JFAXrCmB67JmGgiwJaOEEM1LTjTDnDRLhRvGOMVNS7CqOMO4XHJ6ln2ac7fSip03nfSjcNKIq_ONMH0A3wlMSrasKrYnCV_MuPIuBA_66BAspt7E1Js49paED7OwG5oO2iP-XFQCPj4BMihptZe9MuEfx6opaeLKmXswFsb_XCtubtffCK8nLZ-11Bgcjpr0v0XNKKvEz9tLUdL1d_51xQWlfwE_P8PI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Desmarchelier, Charles ; Tourniaire, Franck ; Prévéraud, Damien P. ; Samson-Kremser, Coralie ; Crenon, Isabelle ; Rosilio, Véronique ; Borel, Patrick</creator><creatorcontrib>Desmarchelier, Charles ; Tourniaire, Franck ; Prévéraud, Damien P. ; Samson-Kremser, Coralie ; Crenon, Isabelle ; Rosilio, Véronique ; Borel, Patrick</creatorcontrib><description>Scope
Vitamin E is present in feed and food mainly as d‐α‐tocopherol (d‐α‐TOL) but also as all‐rac‐α‐tocopheryl acetate (rac‐α‐TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion.
Methods and results
Using an in vitro digestion model, we showed that TAC was distributed between mixed micelles (36%), liposomes (9%), and nonsolubilized food debris (52%). A significant fraction of TAC was also found in emulsions when fat hydrolysis was not complete. Among the candidate esterases tested, i.e. cholesteryl ester hydrolase, pancreatic lipase, and pancreatic lipase‐related protein 2, only cholesteryl ester hydrolase was able to hydrolyze TAC to all‐rac‐α‐TOL, about five times more efficiently when it was incorporated into mixed micelles or liposomes than into emulsions or in the food matrix. Caco‐2 cells were able to hydrolyze TAC and to uptake TOL when TAC was incorporated into mixed micelles but not into emulsions.
Conclusion
During digestion, most TAC is recovered in matrices where its hydrolysis and its uptake by intestinal cells are markedly less efficient than in mixed micelles.</description><identifier>ISSN: 1613-4125</identifier><identifier>EISSN: 1613-4133</identifier><identifier>DOI: 10.1002/mnfr.201200720</identifier><identifier>PMID: 23520193</identifier><language>eng</language><publisher>Weinheim: Blackwell Publishing Ltd</publisher><subject>alpha-Tocopherol - administration & dosage ; alpha-Tocopherol - pharmacokinetics ; Bioaccessibility ; Biological and medical sciences ; Biological Availability ; Caco-2 Cells ; Cells, Cultured ; Cholesteryl ester hydrolase ; Dietary Fats - administration & dosage ; Dietary Fats - analysis ; Dietary Supplements ; Digestion - drug effects ; Edible Grain - chemistry ; Emulsions - metabolism ; Feeding. Feeding behavior ; Food and Nutrition ; Food matrix ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolysis ; Intestinal Absorption ; Intestine, Small - drug effects ; Intestine, Small - metabolism ; Life Sciences ; Lipase - metabolism ; Liposomes - metabolism ; Micelles ; Pancreatic lipase ; Sterol Esterase - metabolism ; Triglycerides - metabolism ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vitamin E</subject><ispartof>Molecular nutrition & food research, 2013-07, Vol.57 (7), p.1237-1245</ispartof><rights>2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2014 INIST-CNRS</rights><rights>2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4123-3bc71d41e627b4e9f467fe1e7873111f9491f7091b8c0b77930bd10c597004893</citedby><cites>FETCH-LOGICAL-c4123-3bc71d41e627b4e9f467fe1e7873111f9491f7091b8c0b77930bd10c597004893</cites><orcidid>0000-0001-9970-7089 ; 0000-0001-9977-3238</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmnfr.201200720$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmnfr.201200720$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27520073$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23520193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-01478557$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Desmarchelier, Charles</creatorcontrib><creatorcontrib>Tourniaire, Franck</creatorcontrib><creatorcontrib>Prévéraud, Damien P.</creatorcontrib><creatorcontrib>Samson-Kremser, Coralie</creatorcontrib><creatorcontrib>Crenon, Isabelle</creatorcontrib><creatorcontrib>Rosilio, Véronique</creatorcontrib><creatorcontrib>Borel, Patrick</creatorcontrib><title>The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability</title><title>Molecular nutrition & food research</title><addtitle>Mol. Nutr. Food Res</addtitle><description>Scope
Vitamin E is present in feed and food mainly as d‐α‐tocopherol (d‐α‐TOL) but also as all‐rac‐α‐tocopheryl acetate (rac‐α‐TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion.
Methods and results
Using an in vitro digestion model, we showed that TAC was distributed between mixed micelles (36%), liposomes (9%), and nonsolubilized food debris (52%). A significant fraction of TAC was also found in emulsions when fat hydrolysis was not complete. Among the candidate esterases tested, i.e. cholesteryl ester hydrolase, pancreatic lipase, and pancreatic lipase‐related protein 2, only cholesteryl ester hydrolase was able to hydrolyze TAC to all‐rac‐α‐TOL, about five times more efficiently when it was incorporated into mixed micelles or liposomes than into emulsions or in the food matrix. Caco‐2 cells were able to hydrolyze TAC and to uptake TOL when TAC was incorporated into mixed micelles but not into emulsions.
Conclusion
During digestion, most TAC is recovered in matrices where its hydrolysis and its uptake by intestinal cells are markedly less efficient than in mixed micelles.</description><subject>alpha-Tocopherol - administration & dosage</subject><subject>alpha-Tocopherol - pharmacokinetics</subject><subject>Bioaccessibility</subject><subject>Biological and medical sciences</subject><subject>Biological Availability</subject><subject>Caco-2 Cells</subject><subject>Cells, Cultured</subject><subject>Cholesteryl ester hydrolase</subject><subject>Dietary Fats - administration & dosage</subject><subject>Dietary Fats - analysis</subject><subject>Dietary Supplements</subject><subject>Digestion - drug effects</subject><subject>Edible Grain - chemistry</subject><subject>Emulsions - metabolism</subject><subject>Feeding. Feeding behavior</subject><subject>Food and Nutrition</subject><subject>Food matrix</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Intestinal Absorption</subject><subject>Intestine, Small - drug effects</subject><subject>Intestine, Small - metabolism</subject><subject>Life Sciences</subject><subject>Lipase - metabolism</subject><subject>Liposomes - metabolism</subject><subject>Micelles</subject><subject>Pancreatic lipase</subject><subject>Sterol Esterase - metabolism</subject><subject>Triglycerides - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vitamin E</subject><issn>1613-4125</issn><issn>1613-4133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v0zAchiMEYmNw5Yh84UaKHSdxfZyqdZvUDQRDHC3H-Xk1OHFlO13z5fhsOMtWjpz873le23qz7D3BC4Jx8bnrtV8UmBQYswK_yE5JTWheEkpfHudFdZK9CeEXxpQUJX2dnRS0Sg6np9mfuy2g1oToTTNE43ok-xZ5sDKaPaDt2Hpnx2ACchpFp9xuC360SCqIMgIyPYqPCVqDhz6iTqYoBQEpB4eUa_r7Z8gOHfSPOWkROmltOokwMSlh8BPamvtpI71DucG2CA47K5NvYkDWPaDGOLmXxsrGWBPHt9krLW2Ad0_jWfZjfXG3uso3Xy6vV-ebXKXv05w2ipG2JFAXrCmB67JmGgiwJaOEEM1LTjTDnDRLhRvGOMVNS7CqOMO4XHJ6ln2ac7fSip03nfSjcNKIq_ONMH0A3wlMSrasKrYnCV_MuPIuBA_66BAspt7E1Js49paED7OwG5oO2iP-XFQCPj4BMihptZe9MuEfx6opaeLKmXswFsb_XCtubtffCK8nLZ-11Bgcjpr0v0XNKKvEz9tLUdL1d_51xQWlfwE_P8PI</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Desmarchelier, Charles</creator><creator>Tourniaire, Franck</creator><creator>Prévéraud, Damien P.</creator><creator>Samson-Kremser, Coralie</creator><creator>Crenon, Isabelle</creator><creator>Rosilio, Véronique</creator><creator>Borel, Patrick</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley-VCH Verlag</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-9970-7089</orcidid><orcidid>https://orcid.org/0000-0001-9977-3238</orcidid></search><sort><creationdate>201307</creationdate><title>The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability</title><author>Desmarchelier, Charles ; Tourniaire, Franck ; Prévéraud, Damien P. ; Samson-Kremser, Coralie ; Crenon, Isabelle ; Rosilio, Véronique ; Borel, Patrick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4123-3bc71d41e627b4e9f467fe1e7873111f9491f7091b8c0b77930bd10c597004893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>alpha-Tocopherol - administration & dosage</topic><topic>alpha-Tocopherol - pharmacokinetics</topic><topic>Bioaccessibility</topic><topic>Biological and medical sciences</topic><topic>Biological Availability</topic><topic>Caco-2 Cells</topic><topic>Cells, Cultured</topic><topic>Cholesteryl ester hydrolase</topic><topic>Dietary Fats - administration & dosage</topic><topic>Dietary Fats - analysis</topic><topic>Dietary Supplements</topic><topic>Digestion - drug effects</topic><topic>Edible Grain - chemistry</topic><topic>Emulsions - metabolism</topic><topic>Feeding. Feeding behavior</topic><topic>Food and Nutrition</topic><topic>Food matrix</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Intestinal Absorption</topic><topic>Intestine, Small - drug effects</topic><topic>Intestine, Small - metabolism</topic><topic>Life Sciences</topic><topic>Lipase - metabolism</topic><topic>Liposomes - metabolism</topic><topic>Micelles</topic><topic>Pancreatic lipase</topic><topic>Sterol Esterase - metabolism</topic><topic>Triglycerides - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vitamin E</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Desmarchelier, Charles</creatorcontrib><creatorcontrib>Tourniaire, Franck</creatorcontrib><creatorcontrib>Prévéraud, Damien P.</creatorcontrib><creatorcontrib>Samson-Kremser, Coralie</creatorcontrib><creatorcontrib>Crenon, Isabelle</creatorcontrib><creatorcontrib>Rosilio, Véronique</creatorcontrib><creatorcontrib>Borel, Patrick</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular nutrition & food research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Desmarchelier, Charles</au><au>Tourniaire, Franck</au><au>Prévéraud, Damien P.</au><au>Samson-Kremser, Coralie</au><au>Crenon, Isabelle</au><au>Rosilio, Véronique</au><au>Borel, Patrick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability</atitle><jtitle>Molecular nutrition & food research</jtitle><addtitle>Mol. Nutr. Food Res</addtitle><date>2013-07</date><risdate>2013</risdate><volume>57</volume><issue>7</issue><spage>1237</spage><epage>1245</epage><pages>1237-1245</pages><issn>1613-4125</issn><eissn>1613-4133</eissn><abstract>Scope
Vitamin E is present in feed and food mainly as d‐α‐tocopherol (d‐α‐TOL) but also as all‐rac‐α‐tocopheryl acetate (rac‐α‐TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion.
Methods and results
Using an in vitro digestion model, we showed that TAC was distributed between mixed micelles (36%), liposomes (9%), and nonsolubilized food debris (52%). A significant fraction of TAC was also found in emulsions when fat hydrolysis was not complete. Among the candidate esterases tested, i.e. cholesteryl ester hydrolase, pancreatic lipase, and pancreatic lipase‐related protein 2, only cholesteryl ester hydrolase was able to hydrolyze TAC to all‐rac‐α‐TOL, about five times more efficiently when it was incorporated into mixed micelles or liposomes than into emulsions or in the food matrix. Caco‐2 cells were able to hydrolyze TAC and to uptake TOL when TAC was incorporated into mixed micelles but not into emulsions.
Conclusion
During digestion, most TAC is recovered in matrices where its hydrolysis and its uptake by intestinal cells are markedly less efficient than in mixed micelles.</abstract><cop>Weinheim</cop><pub>Blackwell Publishing Ltd</pub><pmid>23520193</pmid><doi>10.1002/mnfr.201200720</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9970-7089</orcidid><orcidid>https://orcid.org/0000-0001-9977-3238</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular nutrition & food research, 2013-07, Vol.57 (7), p.1237-1245 |
| issn | 1613-4125 1613-4133 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_inserm_01478557v1 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | alpha-Tocopherol - administration & dosage alpha-Tocopherol - pharmacokinetics Bioaccessibility Biological and medical sciences Biological Availability Caco-2 Cells Cells, Cultured Cholesteryl ester hydrolase Dietary Fats - administration & dosage Dietary Fats - analysis Dietary Supplements Digestion - drug effects Edible Grain - chemistry Emulsions - metabolism Feeding. Feeding behavior Food and Nutrition Food matrix Fundamental and applied biological sciences. Psychology Humans Hydrolysis Intestinal Absorption Intestine, Small - drug effects Intestine, Small - metabolism Life Sciences Lipase - metabolism Liposomes - metabolism Micelles Pancreatic lipase Sterol Esterase - metabolism Triglycerides - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems Vitamin E |
| title | The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability |
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