A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines
► MNP accumulation study in bronchial cells and pulmonary fibroblasts using TEM. ► MNPs are widely and rapidly internalised by both cell types. ► MNPs accumulate chiefly as aggregates in cytosolic vesicles. ► Carbon black and titanium dioxide MNPs have similar accumulation patterns. ► Intracellular...
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| Veröffentlicht in: | Toxicology in vitro 2012-02, Vol.26 (1), p.57-66 |
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| creator | Belade, Esther Armand, Lucie Martinon, Laurent Kheuang, Laurence Fleury-Feith, Jocelyne Baeza-Squiban, Armelle Lanone, Sophie Billon-Galland, Marie-Annick Pairon, Jean-Claude Boczkowski, Jorge |
| description | ► MNP accumulation study in bronchial cells and pulmonary fibroblasts using TEM. ► MNPs are widely and rapidly internalised by both cell types. ► MNPs accumulate chiefly as aggregates in cytosolic vesicles. ► Carbon black and titanium dioxide MNPs have similar accumulation patterns. ► Intracellular MNP accumulation is dissociated from cytotoxicity.
Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO2), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO2 MNPs had similar accumulation patterns. However, TiO2 aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types. |
| doi_str_mv | 10.1016/j.tiv.2011.10.010 |
| format | Article |
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Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO2), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO2 MNPs had similar accumulation patterns. However, TiO2 aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2011.10.010</identifier><identifier>PMID: 22036670</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Biochemistry, Molecular Biology ; Carbon ; Cell Line ; Coloring Agents ; Coloring Agents - metabolism ; Epithelial Cells ; Epithelial Cells - metabolism ; Fibroblasts ; Fibroblasts - metabolism ; Humans ; Life Sciences ; Lung ; Lung - cytology ; Microscopy, Electron, Transmission ; Nanomaterials ; Nanoparticles ; Nanoparticles - ultrastructure ; Nanotechnologies ; Particle Size ; Soot ; Soot - metabolism ; Titanium ; Titanium - metabolism ; Toxicity</subject><ispartof>Toxicology in vitro, 2012-02, Vol.26 (1), p.57-66</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-90acd7bdf78160ed615a6c9385eb8826032c473e8f5e5b51c02331fc415f9ebe3</citedby><cites>FETCH-LOGICAL-c464t-90acd7bdf78160ed615a6c9385eb8826032c473e8f5e5b51c02331fc415f9ebe3</cites><orcidid>0000-0002-4323-6406 ; 0000-0001-6619-5785 ; 0000-0003-2509-8799 ; 0000-0003-2403-8823 ; 0000-0003-4113-3523</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tiv.2011.10.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22036670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-00673352$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Belade, Esther</creatorcontrib><creatorcontrib>Armand, Lucie</creatorcontrib><creatorcontrib>Martinon, Laurent</creatorcontrib><creatorcontrib>Kheuang, Laurence</creatorcontrib><creatorcontrib>Fleury-Feith, Jocelyne</creatorcontrib><creatorcontrib>Baeza-Squiban, Armelle</creatorcontrib><creatorcontrib>Lanone, Sophie</creatorcontrib><creatorcontrib>Billon-Galland, Marie-Annick</creatorcontrib><creatorcontrib>Pairon, Jean-Claude</creatorcontrib><creatorcontrib>Boczkowski, Jorge</creatorcontrib><title>A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>► MNP accumulation study in bronchial cells and pulmonary fibroblasts using TEM. ► MNPs are widely and rapidly internalised by both cell types. ► MNPs accumulate chiefly as aggregates in cytosolic vesicles. ► Carbon black and titanium dioxide MNPs have similar accumulation patterns. ► Intracellular MNP accumulation is dissociated from cytotoxicity.
Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO2), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO2 MNPs had similar accumulation patterns. However, TiO2 aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types.</description><subject>Biochemistry, Molecular Biology</subject><subject>Carbon</subject><subject>Cell Line</subject><subject>Coloring Agents</subject><subject>Coloring Agents - metabolism</subject><subject>Epithelial Cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Fibroblasts</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Lung</subject><subject>Lung - cytology</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanoparticles - ultrastructure</subject><subject>Nanotechnologies</subject><subject>Particle Size</subject><subject>Soot</subject><subject>Soot - metabolism</subject><subject>Titanium</subject><subject>Titanium - metabolism</subject><subject>Toxicity</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP0zAQhS0EYruFH8AF-caFlHHcJI44VSvYRarEBc6W40you44dbKfa_hz-KQ5d9gin8Vjfexq9R8gbBhsGrP5w3CRz2pTAWN43wOAZWTHRtAVnTfOcrECIpig551fkOsYjAFSihJfkqiyB13UDK_JrR7UfJxVUtkKagnJxNDEa7yha1Cnkx2h08FH76Uxjmvsz9QNNJiln5pH2xj-YHqlyPdUqdJnvrNL31Cnns3Ey2mKk85TUPVLj6GEelaN2dj8oTiYd0Bpl_8gH0wWfxTFRjdZSaxzGV-TFoGzE149zTb5__vTt5q7Yf739crPbF3pbb1PRgtJ90_VDI1gN2NesUrVuuaiwE6KsgZd623AUQ4VVVzENORg26C2rhhY75Gvy_uJ7UFZOwYwqnKVXRt7t9tK4iGGUAHXDeVWeWMbfXfAp-J8zxiRzbMvVyqGfo2xLEMBEu_0_ySrggmfjNWEXcok7Bhye7mAgl8blUeaa5NL48pUbz5q3j-5zN2L_pPhbcQY-XgDM4Z0MBhm1QaexNyH3K3tv_mH_G-bQv0Y</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Belade, Esther</creator><creator>Armand, Lucie</creator><creator>Martinon, Laurent</creator><creator>Kheuang, Laurence</creator><creator>Fleury-Feith, Jocelyne</creator><creator>Baeza-Squiban, Armelle</creator><creator>Lanone, Sophie</creator><creator>Billon-Galland, Marie-Annick</creator><creator>Pairon, Jean-Claude</creator><creator>Boczkowski, Jorge</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-4323-6406</orcidid><orcidid>https://orcid.org/0000-0001-6619-5785</orcidid><orcidid>https://orcid.org/0000-0003-2509-8799</orcidid><orcidid>https://orcid.org/0000-0003-2403-8823</orcidid><orcidid>https://orcid.org/0000-0003-4113-3523</orcidid></search><sort><creationdate>201202</creationdate><title>A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines</title><author>Belade, Esther ; Armand, Lucie ; Martinon, Laurent ; Kheuang, Laurence ; Fleury-Feith, Jocelyne ; Baeza-Squiban, Armelle ; Lanone, Sophie ; Billon-Galland, Marie-Annick ; Pairon, Jean-Claude ; Boczkowski, Jorge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-90acd7bdf78160ed615a6c9385eb8826032c473e8f5e5b51c02331fc415f9ebe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biochemistry, Molecular Biology</topic><topic>Carbon</topic><topic>Cell Line</topic><topic>Coloring Agents</topic><topic>Coloring Agents - metabolism</topic><topic>Epithelial Cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Fibroblasts</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Lung</topic><topic>Lung - cytology</topic><topic>Microscopy, Electron, Transmission</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanoparticles - ultrastructure</topic><topic>Nanotechnologies</topic><topic>Particle Size</topic><topic>Soot</topic><topic>Soot - metabolism</topic><topic>Titanium</topic><topic>Titanium - metabolism</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belade, Esther</creatorcontrib><creatorcontrib>Armand, Lucie</creatorcontrib><creatorcontrib>Martinon, Laurent</creatorcontrib><creatorcontrib>Kheuang, Laurence</creatorcontrib><creatorcontrib>Fleury-Feith, Jocelyne</creatorcontrib><creatorcontrib>Baeza-Squiban, Armelle</creatorcontrib><creatorcontrib>Lanone, Sophie</creatorcontrib><creatorcontrib>Billon-Galland, Marie-Annick</creatorcontrib><creatorcontrib>Pairon, Jean-Claude</creatorcontrib><creatorcontrib>Boczkowski, Jorge</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belade, Esther</au><au>Armand, Lucie</au><au>Martinon, Laurent</au><au>Kheuang, Laurence</au><au>Fleury-Feith, Jocelyne</au><au>Baeza-Squiban, Armelle</au><au>Lanone, Sophie</au><au>Billon-Galland, Marie-Annick</au><au>Pairon, Jean-Claude</au><au>Boczkowski, Jorge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2012-02</date><risdate>2012</risdate><volume>26</volume><issue>1</issue><spage>57</spage><epage>66</epage><pages>57-66</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>► MNP accumulation study in bronchial cells and pulmonary fibroblasts using TEM. ► MNPs are widely and rapidly internalised by both cell types. ► MNPs accumulate chiefly as aggregates in cytosolic vesicles. ► Carbon black and titanium dioxide MNPs have similar accumulation patterns. ► Intracellular MNP accumulation is dissociated from cytotoxicity.
Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO2), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO2 MNPs had similar accumulation patterns. However, TiO2 aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22036670</pmid><doi>10.1016/j.tiv.2011.10.010</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4323-6406</orcidid><orcidid>https://orcid.org/0000-0001-6619-5785</orcidid><orcidid>https://orcid.org/0000-0003-2509-8799</orcidid><orcidid>https://orcid.org/0000-0003-2403-8823</orcidid><orcidid>https://orcid.org/0000-0003-4113-3523</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry, Molecular Biology Carbon Cell Line Coloring Agents Coloring Agents - metabolism Epithelial Cells Epithelial Cells - metabolism Fibroblasts Fibroblasts - metabolism Humans Life Sciences Lung Lung - cytology Microscopy, Electron, Transmission Nanomaterials Nanoparticles Nanoparticles - ultrastructure Nanotechnologies Particle Size Soot Soot - metabolism Titanium Titanium - metabolism Toxicity |
| title | A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines |
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