Nuclear receptor ligand-binding domains: reduction of helix H12 dynamics to favour crystallization

Crystallization trials of the human retinoid X receptor alpha ligand-binding domain (RXRalpha LBD) in complex with various ligands have been carried out. Using fluorescence anisotropy, it has been found that when compared with agonists these small-molecule effectors enhance the dynamics of the RXRal...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2008-07, Vol.64 (Pt 7), p.614-6
Hauptverfasser:	Nahoum, Virginie, Lipski, Alexandra, Quillard, Fabien, Guichou, Jean François, Boublik, Yvan, Pérez, Efrèn, Germain, Pierre, de Lera, Angel R., Bourguet, William
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Biochemistry, Molecular Biology Crystallization Fluorescence Polarization Fluorescent Dyes Humans Life Sciences Ligands Predictive Value of Tests Protein Binding Protein Structure, Secondary Protein Structure, Tertiary Receptors, Cytoplasmic and Nuclear Retinoid X Receptor alpha
Online-Zugang:	Volltext
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