Fluorescence time-lapse imaging of single cells targeted with a focused scanning charged-particle microbeam
Charged particle microbeams provide unique features to study targeted and non-targeted radiation response and have recently emerged as a powerful tool to investigate radiation-induced DNA damage and repair. We have developed a charged particle microbeam delivering protons and alpha particles in the...
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| Veröffentlicht in: | Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2014-04, Vol.325, p.27-34 |
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| container_title | Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms |
| container_volume | 325 |
| creator | Bourret, Stéphane Vianna, François Devès, Guillaume Atallah, Vincent Moretto, Philippe Seznec, Hervé Barberet, Philippe |
| description | Charged particle microbeams provide unique features to study targeted and non-targeted radiation response and have recently emerged as a powerful tool to investigate radiation-induced DNA damage and repair. We have developed a charged particle microbeam delivering protons and alpha particles in the MeV energy range equipped with online time-lapse imaging capabilities. The beam is focused to a sub-micrometer beam spot under vacuum by means of a triplet of magnetic quadrupoles and extracted in air through a 200nm Si3N4 window. The end-station is equipped with an automated fluorescence microscope used for single cell targeting and online time-lapse imaging. Cells are kept in their medium during the irradiation procedure and the sample temperature is regulated to 37°C. An overall targeting accuracy of 2.0±0.7μm has been measured by tracking the re-localization of the XRCC1 protein. First measurements of this re-localization shows the ability of our system to follow online the radiation-induced re-localization of proteins in the first minutes after irradiation. |
| doi_str_mv | 10.1016/j.nimb.2014.02.004 |
| format | Article |
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First measurements of this re-localization shows the ability of our system to follow online the radiation-induced re-localization of proteins in the first minutes after irradiation.</description><subject>Instrumentation and Detectors</subject><subject>Microbeam</subject><subject>Physics</subject><subject>Radiation biology</subject><subject>Single cell</subject><subject>Targeted irradiation</subject><subject>Time-lapse microscopy</subject><issn>0168-583X</issn><issn>1872-9584</issn><issn>1872-9584</issn><issn>0168-583X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEUhYMoWB9_wFX2MmMek-kE3JRirVBwo-AuZJKbNnVeJNOK_94MFZfezeHC-S73HITuKMkpoeXDPu98W-eM0CInLCekOEMzWs1ZJkVVnKNZMlWZqPjHJbqKcU_SCC5m6HPVHPoA0UBnAI--hazRQwTsW7313Rb3DsekDWADTRPxqMMWRrD4y487rLHrzSGmNRrddRNgdpPDZoMOozeJa70JfQ26vUEXTjcRbn_1Gr2vnt6W62zz-vyyXGwyw3k5Zq7m4OYAjHPHpHPWUUuspBWpXCltUfG6nNeMG0GFtdxUppRSWk1kzS1jkl-j-9PdnW7UEFKS8K167dV6sVG-YwNXhEhRFkIcaXKzkzt9GWMA94dQoqZy1V5N5aqpXEVYYosEPZ4gSDmOHoKKxk8VWh_AjMr2_j_8Bww2hGs</recordid><startdate>20140415</startdate><enddate>20140415</enddate><creator>Bourret, Stéphane</creator><creator>Vianna, François</creator><creator>Devès, Guillaume</creator><creator>Atallah, Vincent</creator><creator>Moretto, Philippe</creator><creator>Seznec, Hervé</creator><creator>Barberet, Philippe</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-1326-8140</orcidid><orcidid>https://orcid.org/0000-0001-6180-8925</orcidid><orcidid>https://orcid.org/0000-0002-3401-8351</orcidid></search><sort><creationdate>20140415</creationdate><title>Fluorescence time-lapse imaging of single cells targeted with a focused scanning charged-particle microbeam</title><author>Bourret, Stéphane ; Vianna, François ; Devès, Guillaume ; Atallah, Vincent ; Moretto, Philippe ; Seznec, Hervé ; Barberet, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-fb3ef7ee233f29ffdf1d0d91808f69d483b67b23c515dd3c8c6999da09b3d2293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Instrumentation and Detectors</topic><topic>Microbeam</topic><topic>Physics</topic><topic>Radiation biology</topic><topic>Single cell</topic><topic>Targeted irradiation</topic><topic>Time-lapse microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bourret, Stéphane</creatorcontrib><creatorcontrib>Vianna, François</creatorcontrib><creatorcontrib>Devès, Guillaume</creatorcontrib><creatorcontrib>Atallah, Vincent</creatorcontrib><creatorcontrib>Moretto, Philippe</creatorcontrib><creatorcontrib>Seznec, Hervé</creatorcontrib><creatorcontrib>Barberet, Philippe</creatorcontrib><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Nuclear instruments & methods in physics research. 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| identifier | ISSN: 0168-583X |
| ispartof | Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 2014-04, Vol.325, p.27-34 |
| issn | 0168-583X 1872-9584 1872-9584 0168-583X |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Instrumentation and Detectors Microbeam Physics Radiation biology Single cell Targeted irradiation Time-lapse microscopy |
| title | Fluorescence time-lapse imaging of single cells targeted with a focused scanning charged-particle microbeam |
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