Role of the Middle Residue in the Triple Tryptophan Electron Transfer Chain of DNA Photolyase:  Ultrafast Spectroscopy of a Trp→Phe Mutant

Photoreduction of the semi-reduced flavin adenine dinucleotide cofactor FADH• in DNA photolyase from Escherichia coli into FADH- involves three tryptophan (W) residues that form a closely spaced electron-transfer chain FADH•−W382−W359−W306. To investigate this process, we have constructed a mutant p...

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Veröffentlicht in:	The journal of physical chemistry. B 2006-08, Vol.110 (32), p.15654-15658
Hauptverfasser:	Lukacs, Andras, Eker, André P. M, Byrdin, Martin, Villette, Sandrine, Pan, Jie, Brettel, Klaus, Vos, Marten H
Format:	Artikel
Sprache:	eng
Schlagworte:	Deoxyribodipyrimidine Photo-Lyase - chemistry Deoxyribodipyrimidine Photo-Lyase - isolation & purification Electron Transport Escherichia coli - chemistry Flavin-Adenine Dinucleotide - chemistry Free Radicals - chemistry Mutation Phenylalanine - chemistry Sensitivity and Specificity Spectrophotometry, Ultraviolet - methods Tryptophan - chemistry
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