Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis
Among six tested polyphenolic compounds from Clusiaceae, the two endothelium-dependent vasodilatators isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) showed paradoxical effects on in vitro angiogenesis, IA being pro-angiogenic and DRX anti-angiogenic. Clusiaceae plants display high conte...
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| Veröffentlicht in: | Biochemical pharmacology 2012-02, Vol.83 (4), p.514-523 |
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| creator | Lavaud, Alexis Soleti, Raffaella Hay, Anne-Emmanuelle Richomme, Pascal Guilet, David Andriantsitohaina, Ramaroson |
| description | Among six tested polyphenolic compounds from Clusiaceae, the two endothelium-dependent vasodilatators isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) showed paradoxical effects on
in vitro angiogenesis, IA being pro-angiogenic and DRX anti-angiogenic.
Clusiaceae plants display high contents of xanthones and coumarins, the effects of which on endothelium, more particularly on angiogenesis, have not been assessed yet. We screened the capacity of six molecules from Clusiaceae – belonging to xanthones, coumarins and acid chromanes classes – to induce endothelium-dependent relaxation on mice aortic rings. Endothelial nitric oxide (NO) production was assessed in endothelial cell line using electron paramagnetic resonance technique. Then, the capacity of these molecules to induce capillary-like structures of endothelial cells was assessed. Cellular processes implicated in angiogenesis (adhesion, migration and proliferation) and Western blot analyses were then investigated. Among the tested molecules, isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) induced an endothelium-dependent relaxation of the aorta associated with an increase of NO production in endothelial cells. Using
in vitro and
ex vivo angiogenesis assays, it was shown that IA treatment promoted the formation of capillary-like network. In contrast, DRX prevented the ability of vascular endothelial growth factor (VEGF) to increase the formation of capillary-like network. IA increased endothelial cell proliferation while DRX decreased all cellular processes of angiogenesis. Western blot analysis showed that IA increased VEGF expression whereas DRX decreased ICAM-1 expression. Altogether, these data allowed identifying isolated molecules from Clusiaceae that exhibit a potential activity towards the modulation of endothelium-dependent relaxation involving NO release. Interestingly, they also highlighted paradoxical effects of the two compounds on cellular angiogenic processes, IA being pro-angiogenic and DRX anti-angiogenic. |
| doi_str_mv | 10.1016/j.bcp.2011.12.002 |
| format | Article |
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in vitro angiogenesis, IA being pro-angiogenic and DRX anti-angiogenic.
Clusiaceae plants display high contents of xanthones and coumarins, the effects of which on endothelium, more particularly on angiogenesis, have not been assessed yet. We screened the capacity of six molecules from Clusiaceae – belonging to xanthones, coumarins and acid chromanes classes – to induce endothelium-dependent relaxation on mice aortic rings. Endothelial nitric oxide (NO) production was assessed in endothelial cell line using electron paramagnetic resonance technique. Then, the capacity of these molecules to induce capillary-like structures of endothelial cells was assessed. Cellular processes implicated in angiogenesis (adhesion, migration and proliferation) and Western blot analyses were then investigated. Among the tested molecules, isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) induced an endothelium-dependent relaxation of the aorta associated with an increase of NO production in endothelial cells. Using
in vitro and
ex vivo angiogenesis assays, it was shown that IA treatment promoted the formation of capillary-like network. In contrast, DRX prevented the ability of vascular endothelial growth factor (VEGF) to increase the formation of capillary-like network. IA increased endothelial cell proliferation while DRX decreased all cellular processes of angiogenesis. Western blot analysis showed that IA increased VEGF expression whereas DRX decreased ICAM-1 expression. Altogether, these data allowed identifying isolated molecules from Clusiaceae that exhibit a potential activity towards the modulation of endothelium-dependent relaxation involving NO release. Interestingly, they also highlighted paradoxical effects of the two compounds on cellular angiogenic processes, IA being pro-angiogenic and DRX anti-angiogenic.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/j.bcp.2011.12.002</identifier><identifier>PMID: 22177987</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid ; adhesion ; Angiogenesis ; Animals ; aorta ; Aorta - drug effects ; Biodiversity and Ecology ; Blotting, Western ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Clusiaceae ; Clusiaceae - chemistry ; coumarins ; electron paramagnetic resonance spectroscopy ; endothelial cells ; Endothelium ; Endothelium, Vascular - drug effects ; Environmental Sciences ; Humans ; Life Sciences ; Male ; Mice ; Molecular Structure ; Neovascularization, Physiologic - drug effects ; Nitric oxide ; Nitric Oxide - metabolism ; Pharmaceutical sciences ; Pharmacology ; polyphenols ; Polyphenols - chemistry ; Polyphenols - pharmacology ; Tissue Culture Techniques ; vascular endothelial growth factors ; Western blotting ; Xanthone ; xanthones</subject><ispartof>Biochemical pharmacology, 2012-02, Vol.83 (4), p.514-523</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-2e0f8a89cc3f36daedb75510b440e0a1de33c057b6354cc58aecd4d8467f8b583</citedby><cites>FETCH-LOGICAL-c479t-2e0f8a89cc3f36daedb75510b440e0a1de33c057b6354cc58aecd4d8467f8b583</cites><orcidid>0000-0001-5538-0934 ; 0000-0003-2828-7755 ; 0000-0001-7788-2556</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006295211008963$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22177987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/halsde-00722703$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Lavaud, Alexis</creatorcontrib><creatorcontrib>Soleti, Raffaella</creatorcontrib><creatorcontrib>Hay, Anne-Emmanuelle</creatorcontrib><creatorcontrib>Richomme, Pascal</creatorcontrib><creatorcontrib>Guilet, David</creatorcontrib><creatorcontrib>Andriantsitohaina, Ramaroson</creatorcontrib><title>Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>Among six tested polyphenolic compounds from Clusiaceae, the two endothelium-dependent vasodilatators isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) showed paradoxical effects on
in vitro angiogenesis, IA being pro-angiogenic and DRX anti-angiogenic.
Clusiaceae plants display high contents of xanthones and coumarins, the effects of which on endothelium, more particularly on angiogenesis, have not been assessed yet. We screened the capacity of six molecules from Clusiaceae – belonging to xanthones, coumarins and acid chromanes classes – to induce endothelium-dependent relaxation on mice aortic rings. Endothelial nitric oxide (NO) production was assessed in endothelial cell line using electron paramagnetic resonance technique. Then, the capacity of these molecules to induce capillary-like structures of endothelial cells was assessed. Cellular processes implicated in angiogenesis (adhesion, migration and proliferation) and Western blot analyses were then investigated. Among the tested molecules, isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) induced an endothelium-dependent relaxation of the aorta associated with an increase of NO production in endothelial cells. Using
in vitro and
ex vivo angiogenesis assays, it was shown that IA treatment promoted the formation of capillary-like network. In contrast, DRX prevented the ability of vascular endothelial growth factor (VEGF) to increase the formation of capillary-like network. IA increased endothelial cell proliferation while DRX decreased all cellular processes of angiogenesis. Western blot analysis showed that IA increased VEGF expression whereas DRX decreased ICAM-1 expression. Altogether, these data allowed identifying isolated molecules from Clusiaceae that exhibit a potential activity towards the modulation of endothelium-dependent relaxation involving NO release. Interestingly, they also highlighted paradoxical effects of the two compounds on cellular angiogenic processes, IA being pro-angiogenic and DRX anti-angiogenic.</description><subject>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid</subject><subject>adhesion</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>aorta</subject><subject>Aorta - drug effects</subject><subject>Biodiversity and Ecology</subject><subject>Blotting, Western</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Clusiaceae</subject><subject>Clusiaceae - chemistry</subject><subject>coumarins</subject><subject>electron paramagnetic resonance spectroscopy</subject><subject>endothelial cells</subject><subject>Endothelium</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Environmental Sciences</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Structure</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacology</subject><subject>polyphenols</subject><subject>Polyphenols - chemistry</subject><subject>Polyphenols - pharmacology</subject><subject>Tissue Culture Techniques</subject><subject>vascular endothelial growth factors</subject><subject>Western blotting</subject><subject>Xanthone</subject><subject>xanthones</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi1ERZfCA3CB3HrppmMnjh1xqlYtRdqKStCz5djjrVdJHOxNRd8er1J65OTx6JvP1j-EfKJQUqDN5b7szFQyoLSkrARgb8iKSlGtWdvIt2QFAE2uOTsl71PaH6-yoe_IKWNUiFaKFbm711Hb8Mcb3RfoHJpDKoIrptA_T484ht6bwoRhCvNoU-FiGIpNPyevDWoswljocefDDkdMPn0gJ073CT--nGfk4eb61-Z2vf3x7fvmars2tWgPa4bgpJatMZWrGqvRdoJzCl1dA4KmFqvKABddU_HaGC41GltbWTfCyY7L6oxcLN5H3asp-kHHZxW0V7dXW5V7yaICEIwJqJ5oxs8XfIrh94zpoAafDPa9HjHMSbW04UxwqDNJF9LEkFJE92qnoI6Zq73Kmatj5oqy_AjLM59f7HM3oH2d-BdyBr4sgNNB6V30ST38zAaeF8IYZ0fF14XAHNqTx6iS8TgatD7mjSgb_H8-8Bfi-5r7</recordid><startdate>20120215</startdate><enddate>20120215</enddate><creator>Lavaud, Alexis</creator><creator>Soleti, Raffaella</creator><creator>Hay, Anne-Emmanuelle</creator><creator>Richomme, Pascal</creator><creator>Guilet, David</creator><creator>Andriantsitohaina, Ramaroson</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5538-0934</orcidid><orcidid>https://orcid.org/0000-0003-2828-7755</orcidid><orcidid>https://orcid.org/0000-0001-7788-2556</orcidid></search><sort><creationdate>20120215</creationdate><title>Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis</title><author>Lavaud, Alexis ; Soleti, Raffaella ; Hay, Anne-Emmanuelle ; Richomme, Pascal ; Guilet, David ; Andriantsitohaina, Ramaroson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-2e0f8a89cc3f36daedb75510b440e0a1de33c057b6354cc58aecd4d8467f8b583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid</topic><topic>adhesion</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>aorta</topic><topic>Aorta - drug effects</topic><topic>Biodiversity and Ecology</topic><topic>Blotting, Western</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Clusiaceae</topic><topic>Clusiaceae - chemistry</topic><topic>coumarins</topic><topic>electron paramagnetic resonance spectroscopy</topic><topic>endothelial cells</topic><topic>Endothelium</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Environmental Sciences</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Structure</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Pharmaceutical sciences</topic><topic>Pharmacology</topic><topic>polyphenols</topic><topic>Polyphenols - chemistry</topic><topic>Polyphenols - pharmacology</topic><topic>Tissue Culture Techniques</topic><topic>vascular endothelial growth factors</topic><topic>Western blotting</topic><topic>Xanthone</topic><topic>xanthones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lavaud, Alexis</creatorcontrib><creatorcontrib>Soleti, Raffaella</creatorcontrib><creatorcontrib>Hay, Anne-Emmanuelle</creatorcontrib><creatorcontrib>Richomme, Pascal</creatorcontrib><creatorcontrib>Guilet, David</creatorcontrib><creatorcontrib>Andriantsitohaina, Ramaroson</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lavaud, Alexis</au><au>Soleti, Raffaella</au><au>Hay, Anne-Emmanuelle</au><au>Richomme, Pascal</au><au>Guilet, David</au><au>Andriantsitohaina, Ramaroson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>2012-02-15</date><risdate>2012</risdate><volume>83</volume><issue>4</issue><spage>514</spage><epage>523</epage><pages>514-523</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><abstract>Among six tested polyphenolic compounds from Clusiaceae, the two endothelium-dependent vasodilatators isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) showed paradoxical effects on
in vitro angiogenesis, IA being pro-angiogenic and DRX anti-angiogenic.
Clusiaceae plants display high contents of xanthones and coumarins, the effects of which on endothelium, more particularly on angiogenesis, have not been assessed yet. We screened the capacity of six molecules from Clusiaceae – belonging to xanthones, coumarins and acid chromanes classes – to induce endothelium-dependent relaxation on mice aortic rings. Endothelial nitric oxide (NO) production was assessed in endothelial cell line using electron paramagnetic resonance technique. Then, the capacity of these molecules to induce capillary-like structures of endothelial cells was assessed. Cellular processes implicated in angiogenesis (adhesion, migration and proliferation) and Western blot analyses were then investigated. Among the tested molecules, isocalolongic acid (IA) and 2-deprenylrheediaxanthone (DRX) induced an endothelium-dependent relaxation of the aorta associated with an increase of NO production in endothelial cells. Using
in vitro and
ex vivo angiogenesis assays, it was shown that IA treatment promoted the formation of capillary-like network. In contrast, DRX prevented the ability of vascular endothelial growth factor (VEGF) to increase the formation of capillary-like network. IA increased endothelial cell proliferation while DRX decreased all cellular processes of angiogenesis. Western blot analysis showed that IA increased VEGF expression whereas DRX decreased ICAM-1 expression. Altogether, these data allowed identifying isolated molecules from Clusiaceae that exhibit a potential activity towards the modulation of endothelium-dependent relaxation involving NO release. Interestingly, they also highlighted paradoxical effects of the two compounds on cellular angiogenic processes, IA being pro-angiogenic and DRX anti-angiogenic.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>22177987</pmid><doi>10.1016/j.bcp.2011.12.002</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5538-0934</orcidid><orcidid>https://orcid.org/0000-0003-2828-7755</orcidid><orcidid>https://orcid.org/0000-0001-7788-2556</orcidid></addata></record> |
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| subjects | 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid adhesion Angiogenesis Animals aorta Aorta - drug effects Biodiversity and Ecology Blotting, Western Cell Adhesion Cell Movement Cell Proliferation Cells, Cultured Clusiaceae Clusiaceae - chemistry coumarins electron paramagnetic resonance spectroscopy endothelial cells Endothelium Endothelium, Vascular - drug effects Environmental Sciences Humans Life Sciences Male Mice Molecular Structure Neovascularization, Physiologic - drug effects Nitric oxide Nitric Oxide - metabolism Pharmaceutical sciences Pharmacology polyphenols Polyphenols - chemistry Polyphenols - pharmacology Tissue Culture Techniques vascular endothelial growth factors Western blotting Xanthone xanthones |
| title | Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis |
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