Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease
The conventional classification of mature B cells overlooks the diversity within IgD+ CD27− naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and ti...
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| Veröffentlicht in: | Clinical immunology (Orlando, Fla.) Fla.), 2024-11, Vol.268, p.110378, Article 110378 |
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| container_title | Clinical immunology (Orlando, Fla.) |
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| creator | Boudigou, Marina Frutoso, Marie Hémon, Patrice Le Dantec, Christelle Chatzis, Loukas Devauchelle, Valérie Jamin, Christophe Cornec, Divi Pers, Jacques-Olivier Le Pottier, Laëtitia Hillion, Sophie |
| description | The conventional classification of mature B cells overlooks the diversity within IgD+ CD27− naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease.
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•.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures. |
| doi_str_mv | 10.1016/j.clim.2024.110378 |
| format | Article |
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•.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures.</description><identifier>ISSN: 1521-6616</identifier><identifier>ISSN: 1521-7035</identifier><identifier>EISSN: 1521-7035</identifier><identifier>DOI: 10.1016/j.clim.2024.110378</identifier><identifier>PMID: 39393568</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; B-Lymphocytes - immunology ; CD45RB+CD45RAhigh IgD+CD27− human B cells ; Cell Differentiation - immunology ; Female ; High parameter imaging mass cytometry ; Humans ; Immunoglobulin M - immunology ; Immunology ; Inflamed tissues ; Leukocyte Common Antigens - genetics ; Leukocyte Common Antigens - immunology ; Leukocyte Common Antigens - metabolism ; Life Sciences ; Male ; Middle Aged ; Phenotype ; Salivary Glands - immunology ; Sjogren's Syndrome - genetics ; Sjogren's Syndrome - immunology ; Sjögren's disease ; Transcriptome</subject><ispartof>Clinical immunology (Orlando, Fla.), 2024-11, Vol.268, p.110378, Article 110378</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c271t-ec0ebc7050ba054b62b5357304b4e1f531f9292dd81ef623ff698385d2ebabfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clim.2024.110378$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,782,786,887,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39393568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04735103$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Boudigou, Marina</creatorcontrib><creatorcontrib>Frutoso, Marie</creatorcontrib><creatorcontrib>Hémon, Patrice</creatorcontrib><creatorcontrib>Le Dantec, Christelle</creatorcontrib><creatorcontrib>Chatzis, Loukas</creatorcontrib><creatorcontrib>Devauchelle, Valérie</creatorcontrib><creatorcontrib>Jamin, Christophe</creatorcontrib><creatorcontrib>Cornec, Divi</creatorcontrib><creatorcontrib>Pers, Jacques-Olivier</creatorcontrib><creatorcontrib>Le Pottier, Laëtitia</creatorcontrib><creatorcontrib>Hillion, Sophie</creatorcontrib><title>Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease</title><title>Clinical immunology (Orlando, Fla.)</title><addtitle>Clin Immunol</addtitle><description>The conventional classification of mature B cells overlooks the diversity within IgD+ CD27− naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease.
[Display omitted]
•.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures.</description><subject>Adult</subject><subject>B-Lymphocytes - immunology</subject><subject>CD45RB+CD45RAhigh IgD+CD27− human B cells</subject><subject>Cell Differentiation - immunology</subject><subject>Female</subject><subject>High parameter imaging mass cytometry</subject><subject>Humans</subject><subject>Immunoglobulin M - immunology</subject><subject>Immunology</subject><subject>Inflamed tissues</subject><subject>Leukocyte Common Antigens - genetics</subject><subject>Leukocyte Common Antigens - immunology</subject><subject>Leukocyte Common Antigens - metabolism</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Phenotype</subject><subject>Salivary Glands - immunology</subject><subject>Sjogren's Syndrome - genetics</subject><subject>Sjogren's Syndrome - immunology</subject><subject>Sjögren's disease</subject><subject>Transcriptome</subject><issn>1521-6616</issn><issn>1521-7035</issn><issn>1521-7035</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhiMEohd4ARbIO0Awgy-xk0Fs2oFepJFAXNaWYx8zHiV2antGaiWeiV1foC9G0gxdIi9sH33nl875iuIFwXOCiXi_mevWdXOKaTknBLOqflQcEk7JrMKMP96_hSDioDhKaYMx5pSKp8UBWwyHi_qw-P11DT7k697pdyhH5ZOOrs-hG__KG5R6lZ1qkV6rqHSG6G6GQvAoWLT8VPJvp2-RV3d_doA6lbcR0CnS0LbpA7rs-tbpezoh59H3zd3trwj-VULGJVAJnhVPrGoTPN_fx8XPs88_lhez1Zfzy-XJaqZpRfIMNIZGV5jjRmFeNoI2nPGK4bIpgVjOiF3QBTWmJmAFZdaKRc1qbig0qrGGHRdvpty1amUfXafitQzKyYuTlRxruKwYH1a4IwP7emL7GK62kLLsXBonUh7CNklGCK8qjNmI0gnVMaQUwT5kEyxHRXIjR0VyVCQnRUPTy33-tunAPLT8czIAHycAho3sHESZtAOvwbgIOksT3P_y_wKfuaNF</recordid><startdate>20241101</startdate><enddate>20241101</enddate><creator>Boudigou, Marina</creator><creator>Frutoso, Marie</creator><creator>Hémon, Patrice</creator><creator>Le Dantec, Christelle</creator><creator>Chatzis, Loukas</creator><creator>Devauchelle, Valérie</creator><creator>Jamin, Christophe</creator><creator>Cornec, Divi</creator><creator>Pers, Jacques-Olivier</creator><creator>Le Pottier, Laëtitia</creator><creator>Hillion, Sophie</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20241101</creationdate><title>Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease</title><author>Boudigou, Marina ; Frutoso, Marie ; Hémon, Patrice ; Le Dantec, Christelle ; Chatzis, Loukas ; Devauchelle, Valérie ; Jamin, Christophe ; Cornec, Divi ; Pers, Jacques-Olivier ; Le Pottier, Laëtitia ; Hillion, Sophie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-ec0ebc7050ba054b62b5357304b4e1f531f9292dd81ef623ff698385d2ebabfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>B-Lymphocytes - immunology</topic><topic>CD45RB+CD45RAhigh IgD+CD27− human B cells</topic><topic>Cell Differentiation - immunology</topic><topic>Female</topic><topic>High parameter imaging mass cytometry</topic><topic>Humans</topic><topic>Immunoglobulin M - immunology</topic><topic>Immunology</topic><topic>Inflamed tissues</topic><topic>Leukocyte Common Antigens - genetics</topic><topic>Leukocyte Common Antigens - immunology</topic><topic>Leukocyte Common Antigens - metabolism</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Phenotype</topic><topic>Salivary Glands - immunology</topic><topic>Sjogren's Syndrome - genetics</topic><topic>Sjogren's Syndrome - immunology</topic><topic>Sjögren's disease</topic><topic>Transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boudigou, Marina</creatorcontrib><creatorcontrib>Frutoso, Marie</creatorcontrib><creatorcontrib>Hémon, Patrice</creatorcontrib><creatorcontrib>Le Dantec, Christelle</creatorcontrib><creatorcontrib>Chatzis, Loukas</creatorcontrib><creatorcontrib>Devauchelle, Valérie</creatorcontrib><creatorcontrib>Jamin, Christophe</creatorcontrib><creatorcontrib>Cornec, Divi</creatorcontrib><creatorcontrib>Pers, Jacques-Olivier</creatorcontrib><creatorcontrib>Le Pottier, Laëtitia</creatorcontrib><creatorcontrib>Hillion, Sophie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Clinical immunology (Orlando, Fla.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boudigou, Marina</au><au>Frutoso, Marie</au><au>Hémon, Patrice</au><au>Le Dantec, Christelle</au><au>Chatzis, Loukas</au><au>Devauchelle, Valérie</au><au>Jamin, Christophe</au><au>Cornec, Divi</au><au>Pers, Jacques-Olivier</au><au>Le Pottier, Laëtitia</au><au>Hillion, Sophie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease</atitle><jtitle>Clinical immunology (Orlando, Fla.)</jtitle><addtitle>Clin Immunol</addtitle><date>2024-11-01</date><risdate>2024</risdate><volume>268</volume><spage>110378</spage><pages>110378-</pages><artnum>110378</artnum><issn>1521-6616</issn><issn>1521-7035</issn><eissn>1521-7035</eissn><abstract>The conventional classification of mature B cells overlooks the diversity within IgD+ CD27− naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease.
[Display omitted]
•.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39393568</pmid><doi>10.1016/j.clim.2024.110378</doi></addata></record> |
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| subjects | Adult B-Lymphocytes - immunology CD45RB+CD45RAhigh IgD+CD27− human B cells Cell Differentiation - immunology Female High parameter imaging mass cytometry Humans Immunoglobulin M - immunology Immunology Inflamed tissues Leukocyte Common Antigens - genetics Leukocyte Common Antigens - immunology Leukocyte Common Antigens - metabolism Life Sciences Male Middle Aged Phenotype Salivary Glands - immunology Sjogren's Syndrome - genetics Sjogren's Syndrome - immunology Sjögren's disease Transcriptome |
| title | Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease |
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