Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease

The conventional classification of mature B cells overlooks the diversity within IgD+ CD27− naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and ti...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.) Fla.), 2024-11, Vol.268, p.110378, Article 110378
Hauptverfasser: Boudigou, Marina, Frutoso, Marie, Hémon, Patrice, Le Dantec, Christelle, Chatzis, Loukas, Devauchelle, Valérie, Jamin, Christophe, Cornec, Divi, Pers, Jacques-Olivier, Le Pottier, Laëtitia, Hillion, Sophie
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container_title Clinical immunology (Orlando, Fla.)
container_volume 268
creator Boudigou, Marina
Frutoso, Marie
Hémon, Patrice
Le Dantec, Christelle
Chatzis, Loukas
Devauchelle, Valérie
Jamin, Christophe
Cornec, Divi
Pers, Jacques-Olivier
Le Pottier, Laëtitia
Hillion, Sophie
description The conventional classification of mature B cells overlooks the diversity within IgD+ CD27− naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease. [Display omitted] •.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures.
doi_str_mv 10.1016/j.clim.2024.110378
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Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease. [Display omitted] •.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures.</description><identifier>ISSN: 1521-6616</identifier><identifier>ISSN: 1521-7035</identifier><identifier>EISSN: 1521-7035</identifier><identifier>DOI: 10.1016/j.clim.2024.110378</identifier><identifier>PMID: 39393568</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; B-Lymphocytes - immunology ; CD45RB+CD45RAhigh IgD+CD27− human B cells ; Cell Differentiation - immunology ; Female ; High parameter imaging mass cytometry ; Humans ; Immunoglobulin M - immunology ; Immunology ; Inflamed tissues ; Leukocyte Common Antigens - genetics ; Leukocyte Common Antigens - immunology ; Leukocyte Common Antigens - metabolism ; Life Sciences ; Male ; Middle Aged ; Phenotype ; Salivary Glands - immunology ; Sjogren's Syndrome - genetics ; Sjogren's Syndrome - immunology ; Sjögren's disease ; Transcriptome</subject><ispartof>Clinical immunology (Orlando, Fla.), 2024-11, Vol.268, p.110378, Article 110378</ispartof><rights>2024</rights><rights>Copyright © 2024. 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Here, to identify distinct mature naïve B cells, we categorized CD45RBMEM55- B cells (NA RB-) and CD45RBMEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease. [Display omitted] •.IgD+ CD45RBMEM55+ (NA RB+) and IgD+ CD45RBMEM55- B cells are phenotypically, transcriptionally, and functionally distinct.•NA RB+ B cells normally circulate between lymphoid organs but are recruited to inflamed salivary glands in Sjögren's Disease.•In Sjögren's Disease, NA RB+ B cells show an activated phenotype, altered migration, and are near ectopic lymphoid structures.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39393568</pmid><doi>10.1016/j.clim.2024.110378</doi></addata></record>
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subjects Adult
B-Lymphocytes - immunology
CD45RB+CD45RAhigh IgD+CD27− human B cells
Cell Differentiation - immunology
Female
High parameter imaging mass cytometry
Humans
Immunoglobulin M - immunology
Immunology
Inflamed tissues
Leukocyte Common Antigens - genetics
Leukocyte Common Antigens - immunology
Leukocyte Common Antigens - metabolism
Life Sciences
Male
Middle Aged
Phenotype
Salivary Glands - immunology
Sjogren's Syndrome - genetics
Sjogren's Syndrome - immunology
Sjögren's disease
Transcriptome
title Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease
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