The Bacillus subtilis ywbD gene encodes RlmQ, the 23S rRNA methyltransferase forming m 7 G2574 in the A-site of the peptidyl transferase center
Ribosomal RNA contains many posttranscriptionally modified nucleosides, particularly in the functional parts of the ribosome. The distribution of these modifications varies from one organism to another. In , the model organism for Gram-positive bacteria, mass spectrometry experiments revealed the pr...
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| Veröffentlicht in: | RNA (Cambridge) 2024-02, Vol.30 (2), p.105-112 |
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| container_title | RNA (Cambridge) |
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| creator | Wolff, Philippe Labar, Geoffray Lechner, Antony Van Elder, Dany Soin, Romuald Gueydan, Cyril Kruys, Véronique Droogmans, Louis Roovers, Martine |
| description | Ribosomal RNA contains many posttranscriptionally modified nucleosides, particularly in the functional parts of the ribosome. The distribution of these modifications varies from one organism to another. In
, the model organism for Gram-positive bacteria, mass spectrometry experiments revealed the presence of 7-methylguanosine (m
G) at position 2574 of the 23S rRNA, which lies in the A-site of the peptidyl transferase center of the large ribosomal subunit. Testing several m
G methyltransferase candidates allowed us to identify the RlmQ enzyme, encoded by the
open reading frame, as the MTase responsible for this modification. The enzyme methylates free RNA and not ribosomal 50S or 70S particles, suggesting that modification occurs in the early steps of ribosome biogenesis. |
| doi_str_mv | 10.1261/rna.079853.123 |
| format | Article |
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, the model organism for Gram-positive bacteria, mass spectrometry experiments revealed the presence of 7-methylguanosine (m
G) at position 2574 of the 23S rRNA, which lies in the A-site of the peptidyl transferase center of the large ribosomal subunit. Testing several m
G methyltransferase candidates allowed us to identify the RlmQ enzyme, encoded by the
open reading frame, as the MTase responsible for this modification. The enzyme methylates free RNA and not ribosomal 50S or 70S particles, suggesting that modification occurs in the early steps of ribosome biogenesis.</description><identifier>ISSN: 1355-8382</identifier><identifier>EISSN: 1469-9001</identifier><identifier>DOI: 10.1261/rna.079853.123</identifier><identifier>PMID: 38071475</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Bacillus subtilis - genetics ; Bacteriology ; Biochemistry, Molecular Biology ; Genomics ; Life Sciences ; Methyltransferases - genetics ; Microbiology and Parasitology ; Peptidyl Transferases - genetics ; RNA - chemistry ; RNA, Ribosomal, 23S - chemistry ; RNA, Ribosomal, 23S - genetics</subject><ispartof>RNA (Cambridge), 2024-02, Vol.30 (2), p.105-112</ispartof><rights>2024 Wolff et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c965-2e9e103d0c7d036265784247a5e361aeba9ce9053bfb166234970120e9e43c813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38071475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04703238$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolff, Philippe</creatorcontrib><creatorcontrib>Labar, Geoffray</creatorcontrib><creatorcontrib>Lechner, Antony</creatorcontrib><creatorcontrib>Van Elder, Dany</creatorcontrib><creatorcontrib>Soin, Romuald</creatorcontrib><creatorcontrib>Gueydan, Cyril</creatorcontrib><creatorcontrib>Kruys, Véronique</creatorcontrib><creatorcontrib>Droogmans, Louis</creatorcontrib><creatorcontrib>Roovers, Martine</creatorcontrib><title>The Bacillus subtilis ywbD gene encodes RlmQ, the 23S rRNA methyltransferase forming m 7 G2574 in the A-site of the peptidyl transferase center</title><title>RNA (Cambridge)</title><addtitle>RNA</addtitle><description>Ribosomal RNA contains many posttranscriptionally modified nucleosides, particularly in the functional parts of the ribosome. The distribution of these modifications varies from one organism to another. In
, the model organism for Gram-positive bacteria, mass spectrometry experiments revealed the presence of 7-methylguanosine (m
G) at position 2574 of the 23S rRNA, which lies in the A-site of the peptidyl transferase center of the large ribosomal subunit. Testing several m
G methyltransferase candidates allowed us to identify the RlmQ enzyme, encoded by the
open reading frame, as the MTase responsible for this modification. The enzyme methylates free RNA and not ribosomal 50S or 70S particles, suggesting that modification occurs in the early steps of ribosome biogenesis.</description><subject>Bacillus subtilis - genetics</subject><subject>Bacteriology</subject><subject>Biochemistry, Molecular Biology</subject><subject>Genomics</subject><subject>Life Sciences</subject><subject>Methyltransferases - genetics</subject><subject>Microbiology and Parasitology</subject><subject>Peptidyl Transferases - genetics</subject><subject>RNA - chemistry</subject><subject>RNA, Ribosomal, 23S - chemistry</subject><subject>RNA, Ribosomal, 23S - genetics</subject><issn>1355-8382</issn><issn>1469-9001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU9PwzAMxSMEYjC4ckS5ItGRxE3THseADWkCMXav0tbdgvpnSjrQPgVfmWyDiZP97PfzwY-QK84GXET8zjZ6wFQSS_AajsgZD6MkSBjjx74HKYMYYtEj5859-CH49SnpQcwUD5U8I9_zJdJ7nZuqWjvq1llnKuPo5it7oAtskGKTtwU6Oqvqt1vaebeAd2pnL0NaY7fcVJ3VjSvRaoe0bG1tmgWtqaJjIVVITbNjhoEzHdK23KkVrjpTbCr6n82x6dBekJNSVw4vf2ufzJ8e56NJMH0dP4-G0yBPIhkITJAzKFiuCgaRiKSKQxEqLREirjHTSY4Jk5CVGY8iAWGiGBfMYyHkMYc-udmfXeoqXVlTa7tJW23SyXCabmcsVAwExJ9b72DvzW3rnMXyAHCWbkNIfQjpPgSvwQPXe2C1zmosDva_r8MPC1CAsg</recordid><startdate>202402</startdate><enddate>202402</enddate><creator>Wolff, Philippe</creator><creator>Labar, Geoffray</creator><creator>Lechner, Antony</creator><creator>Van Elder, Dany</creator><creator>Soin, Romuald</creator><creator>Gueydan, Cyril</creator><creator>Kruys, Véronique</creator><creator>Droogmans, Louis</creator><creator>Roovers, Martine</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>202402</creationdate><title>The Bacillus subtilis ywbD gene encodes RlmQ, the 23S rRNA methyltransferase forming m 7 G2574 in the A-site of the peptidyl transferase center</title><author>Wolff, Philippe ; Labar, Geoffray ; Lechner, Antony ; Van Elder, Dany ; Soin, Romuald ; Gueydan, Cyril ; Kruys, Véronique ; Droogmans, Louis ; Roovers, Martine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c965-2e9e103d0c7d036265784247a5e361aeba9ce9053bfb166234970120e9e43c813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bacillus subtilis - genetics</topic><topic>Bacteriology</topic><topic>Biochemistry, Molecular Biology</topic><topic>Genomics</topic><topic>Life Sciences</topic><topic>Methyltransferases - genetics</topic><topic>Microbiology and Parasitology</topic><topic>Peptidyl Transferases - genetics</topic><topic>RNA - chemistry</topic><topic>RNA, Ribosomal, 23S - chemistry</topic><topic>RNA, Ribosomal, 23S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolff, Philippe</creatorcontrib><creatorcontrib>Labar, Geoffray</creatorcontrib><creatorcontrib>Lechner, Antony</creatorcontrib><creatorcontrib>Van Elder, Dany</creatorcontrib><creatorcontrib>Soin, Romuald</creatorcontrib><creatorcontrib>Gueydan, Cyril</creatorcontrib><creatorcontrib>Kruys, Véronique</creatorcontrib><creatorcontrib>Droogmans, Louis</creatorcontrib><creatorcontrib>Roovers, Martine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>RNA (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolff, Philippe</au><au>Labar, Geoffray</au><au>Lechner, Antony</au><au>Van Elder, Dany</au><au>Soin, Romuald</au><au>Gueydan, Cyril</au><au>Kruys, Véronique</au><au>Droogmans, Louis</au><au>Roovers, Martine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Bacillus subtilis ywbD gene encodes RlmQ, the 23S rRNA methyltransferase forming m 7 G2574 in the A-site of the peptidyl transferase center</atitle><jtitle>RNA (Cambridge)</jtitle><addtitle>RNA</addtitle><date>2024-02</date><risdate>2024</risdate><volume>30</volume><issue>2</issue><spage>105</spage><epage>112</epage><pages>105-112</pages><issn>1355-8382</issn><eissn>1469-9001</eissn><abstract>Ribosomal RNA contains many posttranscriptionally modified nucleosides, particularly in the functional parts of the ribosome. The distribution of these modifications varies from one organism to another. In
, the model organism for Gram-positive bacteria, mass spectrometry experiments revealed the presence of 7-methylguanosine (m
G) at position 2574 of the 23S rRNA, which lies in the A-site of the peptidyl transferase center of the large ribosomal subunit. Testing several m
G methyltransferase candidates allowed us to identify the RlmQ enzyme, encoded by the
open reading frame, as the MTase responsible for this modification. The enzyme methylates free RNA and not ribosomal 50S or 70S particles, suggesting that modification occurs in the early steps of ribosome biogenesis.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>38071475</pmid><doi>10.1261/rna.079853.123</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | RNA (Cambridge), 2024-02, Vol.30 (2), p.105-112 |
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| language | eng |
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| source | MEDLINE; PubMed Central |
| subjects | Bacillus subtilis - genetics Bacteriology Biochemistry, Molecular Biology Genomics Life Sciences Methyltransferases - genetics Microbiology and Parasitology Peptidyl Transferases - genetics RNA - chemistry RNA, Ribosomal, 23S - chemistry RNA, Ribosomal, 23S - genetics |
| title | The Bacillus subtilis ywbD gene encodes RlmQ, the 23S rRNA methyltransferase forming m 7 G2574 in the A-site of the peptidyl transferase center |
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