Dct::lacZ ES Cells: A Novel Cellular Model to Study Melanocyte Determination and Differentiation
Embryonic stem (ES) cells differentiate into various cell lineages in vitro. A procedure was previously designed to promote the differentiation of ES cells towards the melanocyte lineage and to obtain large and reproducible amounts of melanocytes. To elucidate the main events that lead to the develo...
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| Veröffentlicht in: | Pigment cell research 2004-04, Vol.17 (2), p.142-149 |
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| creator | Pla, Patrick Solov'eva, Olga Moore, Robert Alberti, Christophe Kunisada, Takahiro Larue, Lionel |
| description | Embryonic stem (ES) cells differentiate into various cell lineages in vitro. A procedure was previously designed to promote the differentiation of ES cells towards the melanocyte lineage and to obtain large and reproducible amounts of melanocytes. To elucidate the main events that lead to the development of melanocytes in vitro, we used transgenic Dct::lacZ mouse blastocysts to establish ES cell lines expressing the lacZ reporter gene under the control of the Dct promoter. Dct, a melanoblast marker, is expressed just after melanoblast determination in vivo. We evaluated the importance of recruitment, proliferation and differentiation during melanocyte ontogeny after the in vitro differentiation of Dct::lacZ ES cells into melanocytes. We showed that bFGF and cholera toxin induce precocious melanoblast determination, associated with early melanocyte differentiation. Edn3 induced melanoblast proliferation and long‐term melanoblast recruitment, but not precocious determination. The lack of basic Fibroblast Growth Factor (bFGF) and cholera toxin can be partially compensated by Edn3. Thus, Dct::lacZ ES cells can be used as a model to study determination, proliferation and differentiation in the melanocyte lineage in vitro. |
| doi_str_mv | 10.1046/j.1600-0749.2003.00121.x |
| format | Article |
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A procedure was previously designed to promote the differentiation of ES cells towards the melanocyte lineage and to obtain large and reproducible amounts of melanocytes. To elucidate the main events that lead to the development of melanocytes in vitro, we used transgenic Dct::lacZ mouse blastocysts to establish ES cell lines expressing the lacZ reporter gene under the control of the Dct promoter. Dct, a melanoblast marker, is expressed just after melanoblast determination in vivo. We evaluated the importance of recruitment, proliferation and differentiation during melanocyte ontogeny after the in vitro differentiation of Dct::lacZ ES cells into melanocytes. We showed that bFGF and cholera toxin induce precocious melanoblast determination, associated with early melanocyte differentiation. Edn3 induced melanoblast proliferation and long‐term melanoblast recruitment, but not precocious determination. The lack of basic Fibroblast Growth Factor (bFGF) and cholera toxin can be partially compensated by Edn3. Thus, Dct::lacZ ES cells can be used as a model to study determination, proliferation and differentiation in the melanocyte lineage in vitro.</description><identifier>ISSN: 0893-5785</identifier><identifier>EISSN: 1600-0749</identifier><identifier>DOI: 10.1046/j.1600-0749.2003.00121.x</identifier><identifier>PMID: 15016303</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Animals ; Cell Differentiation ; Cell Division ; Cell Line ; Cell Lineage ; Cell Movement ; Chick Embryo ; Embryo, Mammalian ; Embryo, Mammalian - cytology ; Embryonic stem cells ; Endothelin ; Fibroblast Growth Factor 2 ; Fibroblast Growth Factor 2 - metabolism ; Genotype ; In vitro differentiation ; Life Sciences ; Melanoblast ; Melanocytes ; Melanocytes - cytology ; Melanocytes - metabolism ; Mice ; Mice, Transgenic ; Stem Cells ; Stem Cells - cytology ; Time Factors</subject><ispartof>Pigment cell research, 2004-04, Vol.17 (2), p.142-149</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5031-f22c95b55f6786e8ed5c36082b4da6b9997d24de0a71474ab097d2f27087d07e3</citedby><cites>FETCH-LOGICAL-c5031-f22c95b55f6786e8ed5c36082b4da6b9997d24de0a71474ab097d2f27087d07e3</cites><orcidid>0000-0002-5957-2340</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1600-0749.2003.00121.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1600-0749.2003.00121.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15016303$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04700571$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pla, Patrick</creatorcontrib><creatorcontrib>Solov'eva, Olga</creatorcontrib><creatorcontrib>Moore, Robert</creatorcontrib><creatorcontrib>Alberti, Christophe</creatorcontrib><creatorcontrib>Kunisada, Takahiro</creatorcontrib><creatorcontrib>Larue, Lionel</creatorcontrib><title>Dct::lacZ ES Cells: A Novel Cellular Model to Study Melanocyte Determination and Differentiation</title><title>Pigment cell research</title><addtitle>Pigment Cell Res</addtitle><description>Embryonic stem (ES) cells differentiate into various cell lineages in vitro. A procedure was previously designed to promote the differentiation of ES cells towards the melanocyte lineage and to obtain large and reproducible amounts of melanocytes. To elucidate the main events that lead to the development of melanocytes in vitro, we used transgenic Dct::lacZ mouse blastocysts to establish ES cell lines expressing the lacZ reporter gene under the control of the Dct promoter. Dct, a melanoblast marker, is expressed just after melanoblast determination in vivo. We evaluated the importance of recruitment, proliferation and differentiation during melanocyte ontogeny after the in vitro differentiation of Dct::lacZ ES cells into melanocytes. We showed that bFGF and cholera toxin induce precocious melanoblast determination, associated with early melanocyte differentiation. Edn3 induced melanoblast proliferation and long‐term melanoblast recruitment, but not precocious determination. The lack of basic Fibroblast Growth Factor (bFGF) and cholera toxin can be partially compensated by Edn3. Thus, Dct::lacZ ES cells can be used as a model to study determination, proliferation and differentiation in the melanocyte lineage in vitro.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cell Lineage</subject><subject>Cell Movement</subject><subject>Chick Embryo</subject><subject>Embryo, Mammalian</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryonic stem cells</subject><subject>Endothelin</subject><subject>Fibroblast Growth Factor 2</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Genotype</subject><subject>In vitro differentiation</subject><subject>Life Sciences</subject><subject>Melanoblast</subject><subject>Melanocytes</subject><subject>Melanocytes - cytology</subject><subject>Melanocytes - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Stem Cells</subject><subject>Stem Cells - cytology</subject><subject>Time Factors</subject><issn>0893-5785</issn><issn>1600-0749</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUGP0zAQhS0EYsvCX0A-IXFIGMexnVTiULVLF6ldVlvQor0YJ5mIlDReYmdp_z1JU5UrJ3vG33sjzyOEMggZxPLDNmQSIAAVp2EEwEMAFrFw_4xMzg_PyQSSlAdCJeKCvHJu20Mq5fIluWACmOTAJ-THIvfTaW3yB3q1oXOsazelM3pjn7A-ll1tWrq2RV96Sze-Kw50jbVpbH7wSBfosd1VjfGVbahpCrqoyhJbbHx17L0mL0pTO3xzOi_Jt09XX-fXwerL8vN8tgpyAZwFZRTlqciEKKVKJCZYiJxLSKIsLozM0jRVRRQXCEaxWMUmg6FRRgoSVYBCfknej74_Ta0f22pn2oO2ptLXs5UeehArAKHYE-vZdyP72NrfHTqvd5XL-8-aBm3ntGIqkgLiHkxGMG-tcy2WZ2cGekhCb_WwcD0sXA9J6GMSet9L355mdNkOi3_C0-p74OMI_KlqPPy3sb6dr-_6W68PRn3lPO7PetP-0lJxJfT9zVJ_v7-9W8pNoh_4Xxh8pD4</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Pla, Patrick</creator><creator>Solov'eva, Olga</creator><creator>Moore, Robert</creator><creator>Alberti, Christophe</creator><creator>Kunisada, Takahiro</creator><creator>Larue, Lionel</creator><general>Munksgaard International Publishers</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5957-2340</orcidid></search><sort><creationdate>200404</creationdate><title>Dct::lacZ ES Cells: A Novel Cellular Model to Study Melanocyte Determination and Differentiation</title><author>Pla, Patrick ; Solov'eva, Olga ; Moore, Robert ; Alberti, Christophe ; Kunisada, Takahiro ; Larue, Lionel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5031-f22c95b55f6786e8ed5c36082b4da6b9997d24de0a71474ab097d2f27087d07e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Cell Lineage</topic><topic>Cell Movement</topic><topic>Chick Embryo</topic><topic>Embryo, Mammalian</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryonic stem cells</topic><topic>Endothelin</topic><topic>Fibroblast Growth Factor 2</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Genotype</topic><topic>In vitro differentiation</topic><topic>Life Sciences</topic><topic>Melanoblast</topic><topic>Melanocytes</topic><topic>Melanocytes - cytology</topic><topic>Melanocytes - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Stem Cells</topic><topic>Stem Cells - cytology</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Pla, Patrick</creatorcontrib><creatorcontrib>Solov'eva, Olga</creatorcontrib><creatorcontrib>Moore, Robert</creatorcontrib><creatorcontrib>Alberti, Christophe</creatorcontrib><creatorcontrib>Kunisada, Takahiro</creatorcontrib><creatorcontrib>Larue, Lionel</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Pigment cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pla, Patrick</au><au>Solov'eva, Olga</au><au>Moore, Robert</au><au>Alberti, Christophe</au><au>Kunisada, Takahiro</au><au>Larue, Lionel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dct::lacZ ES Cells: A Novel Cellular Model to Study Melanocyte Determination and Differentiation</atitle><jtitle>Pigment cell research</jtitle><addtitle>Pigment Cell Res</addtitle><date>2004-04</date><risdate>2004</risdate><volume>17</volume><issue>2</issue><spage>142</spage><epage>149</epage><pages>142-149</pages><issn>0893-5785</issn><eissn>1600-0749</eissn><abstract>Embryonic stem (ES) cells differentiate into various cell lineages in vitro. A procedure was previously designed to promote the differentiation of ES cells towards the melanocyte lineage and to obtain large and reproducible amounts of melanocytes. To elucidate the main events that lead to the development of melanocytes in vitro, we used transgenic Dct::lacZ mouse blastocysts to establish ES cell lines expressing the lacZ reporter gene under the control of the Dct promoter. Dct, a melanoblast marker, is expressed just after melanoblast determination in vivo. We evaluated the importance of recruitment, proliferation and differentiation during melanocyte ontogeny after the in vitro differentiation of Dct::lacZ ES cells into melanocytes. We showed that bFGF and cholera toxin induce precocious melanoblast determination, associated with early melanocyte differentiation. Edn3 induced melanoblast proliferation and long‐term melanoblast recruitment, but not precocious determination. The lack of basic Fibroblast Growth Factor (bFGF) and cholera toxin can be partially compensated by Edn3. Thus, Dct::lacZ ES cells can be used as a model to study determination, proliferation and differentiation in the melanocyte lineage in vitro.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>15016303</pmid><doi>10.1046/j.1600-0749.2003.00121.x</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-5957-2340</orcidid></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cell Differentiation Cell Division Cell Line Cell Lineage Cell Movement Chick Embryo Embryo, Mammalian Embryo, Mammalian - cytology Embryonic stem cells Endothelin Fibroblast Growth Factor 2 Fibroblast Growth Factor 2 - metabolism Genotype In vitro differentiation Life Sciences Melanoblast Melanocytes Melanocytes - cytology Melanocytes - metabolism Mice Mice, Transgenic Stem Cells Stem Cells - cytology Time Factors |
| title | Dct::lacZ ES Cells: A Novel Cellular Model to Study Melanocyte Determination and Differentiation |
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